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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Biochemical and Molecular Characterization of a Laccase from <italic>Marasmius quercophilus</italic></title><author><name sortKey="Dedeyan, Boghos" sort="Dedeyan, Boghos" uniqKey="Dedeyan B" first="Boghos" last="Dedeyan">Boghos Dedeyan</name><affiliation><nlm:aff id="N0x98c5188.0x9c9d3b0"></nlm:aff></affiliation></author><author><name sortKey="Klonowska, Agnieszka" sort="Klonowska, Agnieszka" uniqKey="Klonowska A" first="Agnieszka" last="Klonowska">Agnieszka Klonowska</name><affiliation><nlm:aff id="N0x98c5188.0x9c9d3b0"></nlm:aff></affiliation></author><author><name sortKey="Tagger, Simone" sort="Tagger, Simone" uniqKey="Tagger S" first="Simone" last="Tagger">Simone Tagger</name><affiliation><nlm:aff id="N0x98c5188.0x9c9d3b0"></nlm:aff></affiliation></author><author><name sortKey="Tron, Thierry" sort="Tron, Thierry" uniqKey="Tron T" first="Thierry" last="Tron">Thierry Tron</name><affiliation><nlm:aff id="N0x98c5188.0x9c9d3b0"></nlm:aff></affiliation></author><author><name sortKey="Iacazio, Gilles" sort="Iacazio, Gilles" uniqKey="Iacazio G" first="Gilles" last="Iacazio">Gilles Iacazio</name><affiliation><nlm:aff id="N0x98c5188.0x9c9d3b0"></nlm:aff></affiliation></author><author><name sortKey="Gil, Gerard" sort="Gil, Gerard" uniqKey="Gil G" first="Gérard" last="Gil">Gérard Gil</name><affiliation><nlm:aff id="N0x98c5188.0x9c9d3b0"></nlm:aff></affiliation></author><author><name sortKey="Le Petit, Jean" sort="Le Petit, Jean" uniqKey="Le Petit J" first="Jean" last="Le Petit">Jean Le Petit</name><affiliation><nlm:aff id="N0x98c5188.0x9c9d3b0"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">10698753</idno><idno type="pmc">91924</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC91924</idno><idno type="RBID">PMC:91924</idno><date when="2000">2000</date><idno type="wicri:Area/Pmc/Corpus">000059</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000059</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Biochemical and Molecular Characterization of a Laccase from <italic>Marasmius quercophilus</italic></title><author><name sortKey="Dedeyan, Boghos" sort="Dedeyan, Boghos" uniqKey="Dedeyan B" first="Boghos" last="Dedeyan">Boghos Dedeyan</name><affiliation><nlm:aff id="N0x98c5188.0x9c9d3b0"></nlm:aff></affiliation></author><author><name sortKey="Klonowska, Agnieszka" sort="Klonowska, Agnieszka" uniqKey="Klonowska A" first="Agnieszka" last="Klonowska">Agnieszka Klonowska</name><affiliation><nlm:aff id="N0x98c5188.0x9c9d3b0"></nlm:aff></affiliation></author><author><name sortKey="Tagger, Simone" sort="Tagger, Simone" uniqKey="Tagger S" first="Simone" last="Tagger">Simone Tagger</name><affiliation><nlm:aff id="N0x98c5188.0x9c9d3b0"></nlm:aff></affiliation></author><author><name sortKey="Tron, Thierry" sort="Tron, Thierry" uniqKey="Tron T" first="Thierry" last="Tron">Thierry Tron</name><affiliation><nlm:aff id="N0x98c5188.0x9c9d3b0"></nlm:aff></affiliation></author><author><name sortKey="Iacazio, Gilles" sort="Iacazio, Gilles" uniqKey="Iacazio G" first="Gilles" last="Iacazio">Gilles Iacazio</name><affiliation><nlm:aff id="N0x98c5188.0x9c9d3b0"></nlm:aff></affiliation></author><author><name sortKey="Gil, Gerard" sort="Gil, Gerard" uniqKey="Gil G" first="Gérard" last="Gil">Gérard Gil</name><affiliation><nlm:aff id="N0x98c5188.0x9c9d3b0"></nlm:aff></affiliation></author><author><name sortKey="Le Petit, Jean" sort="Le Petit, Jean" uniqKey="Le Petit J" first="Jean" last="Le Petit">Jean Le Petit</name><affiliation><nlm:aff id="N0x98c5188.0x9c9d3b0"></nlm:aff></affiliation></author></analytic><series><title level="j">Applied and Environmental Microbiology</title><idno type="ISSN">0099-2240</idno><idno type="eISSN">1098-5336</idno><imprint><date when="2000">2000</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The basidiomycete <italic>Marasmius quercophilus</italic> is commonly found during autumn on the decaying litter of the evergreen oak (<italic>Quercus ilex</italic> L.), a plant characteristic of Mediterranean forest. This white-rot fungus colonizes the leaf surface with rhizomorphs, causing a total bleaching of the leaf. In synthetic liquid media, this white-rot fungus has strong laccase activity. From a three-step chromatographic procedure, we purified a major isoform to homogeneity. The gene encodes a monomeric glycoprotein of approximately 63 kDa, with a 3.6 isoelectric point, that contains 12% carbohydrate. Spectroscopic analysis of the purified enzyme (UV/visible and electron paramagnetic resonance, atomic absorption) confirmed that it belongs to the “blue copper oxidase” family. With syringaldazine as the substrate, the enzyme's pH optimum was 4.5, the optimal temperature was 75°C, and the <italic>K<sub>m</sub></italic> was 7.1 μM. The structural gene, <italic>lac1</italic>, was cloned and sequenced. This gene encodes a 517-amino-acid protein 99% identical to a laccase produced by PM1, an unidentified basidiomycete previously isolated from wastewater from a paper factory in Spain. This similarity may be explained by the ecological distribution of the evergreen oak in Mediterranean forest.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Appl Environ Microbiol</journal-id><journal-id journal-id-type="publisher-id">APPL ENVIRON MICROBIOL</journal-id><journal-title>Applied and Environmental Microbiology</journal-title><issn pub-type="ppub">0099-2240</issn><issn pub-type="epub">1098-5336</issn><publisher><publisher-name>American Society for Microbiology</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">10698753</article-id><article-id pub-id-type="pmc">91924</article-id><article-id pub-id-type="publisher-id">1177</article-id><article-categories><subj-group subj-group-type="heading"><subject>Mycology</subject></subj-group></article-categories><title-group><article-title>Biochemical and Molecular Characterization of a Laccase from <italic>Marasmius quercophilus</italic></article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Dedeyan</surname><given-names>Boghos</given-names></name><xref ref-type="aff" rid="N0x98c5188.0x9c9d3b0">1</xref></contrib><contrib contrib-type="author"><name><surname>Klonowska</surname><given-names>Agnieszka</given-names></name><xref ref-type="aff" rid="N0x98c5188.0x9c9d3b0">1</xref></contrib><contrib contrib-type="author"><name><surname>Tagger</surname><given-names>Simone</given-names></name><xref ref-type="aff" rid="N0x98c5188.0x9c9d3b0">2</xref></contrib><contrib contrib-type="author"><name><surname>Tron</surname><given-names>Thierry</given-names></name><xref ref-type="aff" rid="N0x98c5188.0x9c9d3b0">1</xref></contrib><contrib contrib-type="author"><name><surname>Iacazio</surname><given-names>Gilles</given-names></name><xref ref-type="aff" rid="N0x98c5188.0x9c9d3b0">2</xref></contrib><contrib contrib-type="author"><name><surname>Gil</surname><given-names>Gérard</given-names></name><xref ref-type="aff" rid="N0x98c5188.0x9c9d3b0">1</xref></contrib><contrib contrib-type="author"><name><surname>Le Petit</surname><given-names>Jean</given-names></name><xref ref-type="aff" rid="N0x98c5188.0x9c9d3b0">2</xref><xref ref-type="author-notes" rid="FN150">*</xref></contrib></contrib-group><aff id="N0x98c5188.0x9c9d3b0"> Laboratoire de Bioinorganique Structurale, CNRS UMR 6517,<sup>1</sup> and Laboratoire de Microbiologie, CNRS UPRESA 6116,<sup>2</sup> Faculté des Sciences de St Jérôme, 13397 Marseille, Cedex 20, France</aff><author-notes><fn id="FN150"><label>*</label><p>Corresponding author. Mailing address: Laboratoire de Microbiologie, case 452, Faculté des Sciences de St Jérôme, 13397 Marseille, Cedex 20, France. Phone: 33 4 91 28 85 29. Fax: 33 4 91 28 80 30. E-mail: <email>Jean.Le-Petit@microbio.u-3mrs.fr</email>.</p></fn></author-notes><pub-date pub-type="ppub"><month>3</month><year>2000</year></pub-date><volume>66</volume><issue>3</issue><fpage>925</fpage><lpage>929</lpage><history><date date-type="received"><day>27</day><month>7</month><year>1999</year></date><date date-type="accepted"><day>26</day><month>11</month><year>1999</year></date></history><copyright-statement>Copyright © 2000, American Society for Microbiology</copyright-statement><copyright-year>2000</copyright-year><abstract><p>The basidiomycete <italic>Marasmius quercophilus</italic> is commonly found during autumn on the decaying litter of the evergreen oak (<italic>Quercus ilex</italic> L.), a plant characteristic of Mediterranean forest. This white-rot fungus colonizes the leaf surface with rhizomorphs, causing a total bleaching of the leaf. In synthetic liquid media, this white-rot fungus has strong laccase activity. From a three-step chromatographic procedure, we purified a major isoform to homogeneity. The gene encodes a monomeric glycoprotein of approximately 63 kDa, with a 3.6 isoelectric point, that contains 12% carbohydrate. Spectroscopic analysis of the purified enzyme (UV/visible and electron paramagnetic resonance, atomic absorption) confirmed that it belongs to the “blue copper oxidase” family. With syringaldazine as the substrate, the enzyme's pH optimum was 4.5, the optimal temperature was 75°C, and the <italic>K<sub>m</sub></italic> was 7.1 μM. The structural gene, <italic>lac1</italic>, was cloned and sequenced. This gene encodes a 517-amino-acid protein 99% identical to a laccase produced by PM1, an unidentified basidiomycete previously isolated from wastewater from a paper factory in Spain. This similarity may be explained by the ecological distribution of the evergreen oak in Mediterranean forest.</p></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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