CheneBelgiqueV1, PascalFrancis, Corpus, bibRecord, 000037

Studies of variability in Holm oak (Quercus ilex subsp. ballota [Desf.] Samp.) through acorn protein profile analysis

Identifieur interne : 000037 ( PascalFrancis/Corpus ); précédent : 000036; suivant : 000038

Studies of variability in Holm oak (Quercus ilex subsp. ballota [Desf.] Samp.) through acorn protein profile analysis

Auteurs : José Valero Galvan ; Luis Valledor ; Rafael Ma. Navarro Cerrillo ; Eustaquio Gil Pelegrin ; Jesus V. Jorrin-Novo

Source :

Journal of proteomics : (Print) [ 1874-3919 ] ; 2011.

RBID : Pascal:11-0407788

Descripteurs français

Pascal (Inist)
- Variabilité, Protéine, Profil, Analyse, Protéomique, Quercus ilex.

English descriptors

KwdEn :
- Analysis, Profile, Protein, Proteomics, Quercus ilex, Variability.

Abstract

Studies of variability in Holm oak (Quercus ilex subsp. ballota [Desf.] Samp.), the dominant tree species in the typical Mediterranean forest, have been carried out by using electrophoresis-based proteomic analysis of acorns. Ten populations distributed throughout the Andalusia region have been surveyed. Acorns were sampled from individual trees and proteins extracted from seed flour by using the TCA-acetone precipitation protocol. Extracts were subjected to SDS-PAGE and 2-DE for protein separation, gel images captured, spot or bands quantified, and subjected to statistical analysis (ANOVA, SOM and clustering). Variable bands or spots among populations were subjected to MALDI-TOF/TOF and LC-MS/MS for identification. The protein yield of the used protocol varied among populations, and it was in the 2.92-5.92 mg/g dry weight range. A total of 23 bands were resolved by SDS-PAGE in the 3-35 kDa Mr range, with 8 and 12, out of the total, showing respectively qualitative and quantitative statistically significant differences among populations. Data allowed grouping populations, with groups being correlated according to geographical location and climate conditions, to northern and southern, as well as the discrimination of both mesic and xeric groups. Acom flour extracts from the most distant populations were analyzed by 2-DE, and 56 differential spots were proposed as markers of variability. Identified proteins were classified into two principal categories; storage and stress/defense protein. Besides providing the first reference map of mature acorn seeds, the use of SDS-PAGE and proteomics in characterizing natural biodiversity in forest trees will be discussed.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

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A08	`01`	`1`	`ENG`	`@1 Studies of variability in Holm oak (Quercus ilex subsp. ballota [Desf.] Samp.) through acorn protein profile analysis`
A09	`01`	`1`	`ENG`	`@1 Plant Proteomics in Europe`
A11	`01`	`1`		`@1 VALERO GALVAN (José)`
A11	`02`	`1`		`@1 VALLEDOR (Luis)`
A11	`03`	`1`		`@1 NAVARRO CERRILLO (Rafael Ma.)`
A11	`04`	`1`		`@1 GIL PELEGRIN (Eustaquio)`
A11	`05`	`1`		`@1 JORRIN-NOVO (Jesus V.)`
A12	`01`	`1`		`@1 RENAUT (Jenny) @9 ed.`
A12	`02`	`1`		`@1 SVENSSON (Birte) @9 ed.`
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A12	`04`	`1`		`@1 PANIS (Bart) @9 ed.`
A14	`01`			`@1 Agricultural and Plant Biochemistry and Proteomics Research Group, Dept. of Biochemistry and Molecular Biology, University of Córdoba @2 Córdoba @3 ESP @Z 1 aut. @Z 5 aut.`
A14	`02`			`@1 Dept. of Molecular Systems Biology (MOSYS), University of Vienna @2 Vienna @3 AUT @Z 2 aut.`
A14	`03`			`@1 Department of Forestry Engineering, ETSIAM, University of Cordoba @2 Cordoba @3 ESP @Z 3 aut.`
A14	`04`			`@1 Forestry Resources Department, Agrifood Research and Technology, Centre of Aragon @2 Zaragoza @3 ESP @Z 4 aut.`
A15	`01`			`@1 Laboratory of Tropical Crop Improvement, Department of Biosystems, K. U. Leuven, Kasteelpark Arenberg 13 @2 3001 Heverlee @3 BEL @Z 4 aut.`
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Format Inist (serveur)

NO :	PASCAL 11-0407788 INIST
ET :	Studies of variability in Holm oak (Quercus ilex subsp. ballota [Desf.] Samp.) through acorn protein profile analysis
AU :	VALERO GALVAN (José); VALLEDOR (Luis); NAVARRO CERRILLO (Rafael Ma.); GIL PELEGRIN (Eustaquio); JORRIN-NOVO (Jesus V.); RENAUT (Jenny); SVENSSON (Birte); JORRIN-NOVO (Jesus); PANIS (Bart)
AF :	Agricultural and Plant Biochemistry and Proteomics Research Group, Dept. of Biochemistry and Molecular Biology, University of Córdoba/Córdoba/Espagne (1 aut., 5 aut.); Dept. of Molecular Systems Biology (MOSYS), University of Vienna/Vienna/Autriche (2 aut.); Department of Forestry Engineering, ETSIAM, University of Cordoba/Cordoba/Espagne (3 aut.); Forestry Resources Department, Agrifood Research and Technology, Centre of Aragon/Zaragoza/Espagne (4 aut.); Laboratory of Tropical Crop Improvement, Department of Biosystems, K. U. Leuven, Kasteelpark Arenberg 13/3001 Heverlee/Belgique (4 aut.)
DT :	Publication en série; Niveau analytique
SO :	Journal of proteomics : (Print); ISSN 1874-3919; Royaume-Uni; Da. 2011; Vol. 74; No. 8; Pp. 1244-1255; Bibl. 63 ref.
LA :	Anglais
EA :	Studies of variability in Holm oak (Quercus ilex subsp. ballota [Desf.] Samp.), the dominant tree species in the typical Mediterranean forest, have been carried out by using electrophoresis-based proteomic analysis of acorns. Ten populations distributed throughout the Andalusia region have been surveyed. Acorns were sampled from individual trees and proteins extracted from seed flour by using the TCA-acetone precipitation protocol. Extracts were subjected to SDS-PAGE and 2-DE for protein separation, gel images captured, spot or bands quantified, and subjected to statistical analysis (ANOVA, SOM and clustering). Variable bands or spots among populations were subjected to MALDI-TOF/TOF and LC-MS/MS for identification. The protein yield of the used protocol varied among populations, and it was in the 2.92-5.92 mg/g dry weight range. A total of 23 bands were resolved by SDS-PAGE in the 3-35 kDa Mr range, with 8 and 12, out of the total, showing respectively qualitative and quantitative statistically significant differences among populations. Data allowed grouping populations, with groups being correlated according to geographical location and climate conditions, to northern and southern, as well as the discrimination of both mesic and xeric groups. Acom flour extracts from the most distant populations were analyzed by 2-DE, and 56 differential spots were proposed as markers of variability. Identified proteins were classified into two principal categories; storage and stress/defense protein. Besides providing the first reference map of mature acorn seeds, the use of SDS-PAGE and proteomics in characterizing natural biodiversity in forest trees will be discussed.
CC :	002A04B
FD :	Variabilité; Protéine; Profil; Analyse; Protéomique; Quercus ilex
FG :	Fagaceae; Dicotyledones; Angiospermae; Spermatophyta
ED :	Variability; Protein; Profile; Analysis; Proteomics; Quercus ilex
EG :	Fagaceae; Dicotyledones; Angiospermae; Spermatophyta
SD :	Variabilidad; Proteína; Perfil; Análisis; Proteómica; Quercus ilex
LO :	INIST-18007.354000191153520070
ID :	11-0407788

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Pascal:11-0407788

Le document en format XML

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<front><div type="abstract" xml:lang="en">Studies of variability in Holm oak (Quercus ilex subsp. ballota [Desf.] Samp.), the dominant tree species in the typical Mediterranean forest, have been carried out by using electrophoresis-based proteomic analysis of acorns. Ten populations distributed throughout the Andalusia region have been surveyed. Acorns were sampled from individual trees and proteins extracted from seed flour by using the TCA-acetone precipitation protocol. Extracts were subjected to SDS-PAGE and 2-DE for protein separation, gel images captured, spot or bands quantified, and subjected to statistical analysis (ANOVA, SOM and clustering). Variable bands or spots among populations were subjected to MALDI-TOF/TOF and LC-MS/MS for identification. The protein yield of the used protocol varied among populations, and it was in the 2.92-5.92 mg/g dry weight range. A total of 23 bands were resolved by SDS-PAGE in the 3-35 kDa Mr range, with 8 and 12, out of the total, showing respectively qualitative and quantitative statistically significant differences among populations. Data allowed grouping populations, with groups being correlated according to geographical location and climate conditions, to northern and southern, as well as the discrimination of both mesic and xeric groups. Acom flour extracts from the most distant populations were analyzed by 2-DE, and 56 differential spots were proposed as markers of variability. Identified proteins were classified into two principal categories; storage and stress/defense protein. Besides providing the first reference map of mature acorn seeds, the use of SDS-PAGE and proteomics in characterizing natural biodiversity in forest trees will be discussed.</div>
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<fC03 i1="01" i2="X" l="SPA"><s0>Variabilidad</s0>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Protéine</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Protein</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Proteína</s0>
<s5>02</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Profil</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Profile</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Perfil</s0>
<s5>03</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Analyse</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Analysis</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Análisis</s0>
<s5>04</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Protéomique</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Proteomics</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Proteómica</s0>
<s5>05</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Quercus ilex</s0>
<s2>NS</s2>
<s5>16</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Quercus ilex</s0>
<s2>NS</s2>
<s5>16</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Quercus ilex</s0>
<s2>NS</s2>
<s5>16</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Fagaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Fagaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Fagaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fN21><s1>283</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
<server><NO>PASCAL 11-0407788 INIST</NO>
<ET>Studies of variability in Holm oak (Quercus ilex subsp. ballota [Desf.] Samp.) through acorn protein profile analysis</ET>
<AU>VALERO GALVAN (José); VALLEDOR (Luis); NAVARRO CERRILLO (Rafael Ma.); GIL PELEGRIN (Eustaquio); JORRIN-NOVO (Jesus V.); RENAUT (Jenny); SVENSSON (Birte); JORRIN-NOVO (Jesus); PANIS (Bart)</AU>
<AF>Agricultural and Plant Biochemistry and Proteomics Research Group, Dept. of Biochemistry and Molecular Biology, University of Córdoba/Córdoba/Espagne (1 aut., 5 aut.); Dept. of Molecular Systems Biology (MOSYS), University of Vienna/Vienna/Autriche (2 aut.); Department of Forestry Engineering, ETSIAM, University of Cordoba/Cordoba/Espagne (3 aut.); Forestry Resources Department, Agrifood Research and Technology, Centre of Aragon/Zaragoza/Espagne (4 aut.); Laboratory of Tropical Crop Improvement, Department of Biosystems, K. U. Leuven, Kasteelpark Arenberg 13/3001 Heverlee/Belgique (4 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Journal of proteomics : (Print); ISSN 1874-3919; Royaume-Uni; Da. 2011; Vol. 74; No. 8; Pp. 1244-1255; Bibl. 63 ref.</SO>
<LA>Anglais</LA>
<EA>Studies of variability in Holm oak (Quercus ilex subsp. ballota [Desf.] Samp.), the dominant tree species in the typical Mediterranean forest, have been carried out by using electrophoresis-based proteomic analysis of acorns. Ten populations distributed throughout the Andalusia region have been surveyed. Acorns were sampled from individual trees and proteins extracted from seed flour by using the TCA-acetone precipitation protocol. Extracts were subjected to SDS-PAGE and 2-DE for protein separation, gel images captured, spot or bands quantified, and subjected to statistical analysis (ANOVA, SOM and clustering). Variable bands or spots among populations were subjected to MALDI-TOF/TOF and LC-MS/MS for identification. The protein yield of the used protocol varied among populations, and it was in the 2.92-5.92 mg/g dry weight range. A total of 23 bands were resolved by SDS-PAGE in the 3-35 kDa Mr range, with 8 and 12, out of the total, showing respectively qualitative and quantitative statistically significant differences among populations. Data allowed grouping populations, with groups being correlated according to geographical location and climate conditions, to northern and southern, as well as the discrimination of both mesic and xeric groups. Acom flour extracts from the most distant populations were analyzed by 2-DE, and 56 differential spots were proposed as markers of variability. Identified proteins were classified into two principal categories; storage and stress/defense protein. Besides providing the first reference map of mature acorn seeds, the use of SDS-PAGE and proteomics in characterizing natural biodiversity in forest trees will be discussed.</EA>
<CC>002A04B</CC>
<FD>Variabilité; Protéine; Profil; Analyse; Protéomique; Quercus ilex</FD>
<FG>Fagaceae; Dicotyledones; Angiospermae; Spermatophyta</FG>
<ED>Variability; Protein; Profile; Analysis; Proteomics; Quercus ilex</ED>
<EG>Fagaceae; Dicotyledones; Angiospermae; Spermatophyta</EG>
<SD>Variabilidad; Proteína; Perfil; Análisis; Proteómica; Quercus ilex</SD>
<LO>INIST-18007.354000191153520070</LO>
<ID>11-0407788</ID>
</server>
</inist>
</record>

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Studies of variability in Holm oak (Quercus ilex subsp. ballota [Desf.] Samp.) through acorn protein profile analysis

Studies of variability in Holm oak (Quercus ilex subsp. ballota [Desf.] Samp.) through acorn protein profile analysis

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