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Taxonomic and ecological discrimination of Fagaceae species based on internal transcribed spacer polymerase chain reaction–restriction fragment length polymorphism

Identifieur interne : 000252 ( Ncbi/Curation ); précédent : 000251; suivant : 000253

Taxonomic and ecological discrimination of Fagaceae species based on internal transcribed spacer polymerase chain reaction–restriction fragment length polymorphism

Auteurs : João Paulo Coutinho ; Ana Carvalho ; José Lima-Brito

Source :

RBID : PMC:4294445

Abstract

In 30 Fagaceae individuals from the Castanea, Fagus and Quercus genera, the internal transcribed spacer region (ITS) of the ribosomal DNA (rDNA) was amplified and digested with four restriction enzymes (HaeIII, HpaII, Sau96I, and TaqI), producing ITS PCR-RFLP markers. This technique allowed the discrimination of the Fagaceae species according to genus, infrageneric group and ecological area. This study constitutes the first application of the alternative co-dominant marker system ITS PCR-RFLP in Fagaceae species and proved to be highly suitable for taxonomic and ecological discrimination, constituting a useful molecular tool for taxonomists and forestry researchers.


Url:
DOI: 10.1093/aobpla/plu079
PubMed: 25429047
PubMed Central: 4294445

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PMC:4294445

Le document en format XML

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<name sortKey="Coutinho, Joao Paulo" sort="Coutinho, Joao Paulo" uniqKey="Coutinho J" first="João Paulo" last="Coutinho">João Paulo Coutinho</name>
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<name sortKey="Carvalho, Ana" sort="Carvalho, Ana" uniqKey="Carvalho A" first="Ana" last="Carvalho">Ana Carvalho</name>
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<name sortKey="Lima Brito, Jose" sort="Lima Brito, Jose" uniqKey="Lima Brito J" first="José" last="Lima-Brito">José Lima-Brito</name>
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<p>In 30 Fagaceae individuals from the
<italic>Castanea</italic>
,
<italic>Fagus</italic>
and
<italic>Quercus</italic>
genera, the internal transcribed spacer region (ITS) of the ribosomal DNA (rDNA) was amplified and digested with four restriction enzymes (
<italic>Hae</italic>
III,
<italic>Hpa</italic>
II,
<italic>Sau</italic>
96I, and
<italic>Taq</italic>
I), producing ITS PCR-RFLP markers. This technique allowed the discrimination of the Fagaceae species according to genus, infrageneric group and ecological area. This study constitutes the first application of the alternative co-dominant marker system ITS PCR-RFLP in Fagaceae species and proved to be highly suitable for taxonomic and ecological discrimination, constituting a useful molecular tool for taxonomists and forestry researchers.</p>
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