Reproductive isolation and hybridization in sympatric populations of three Dactylorhiza species (Orchidaceae) with different ploidy levels
Identifieur interne : 000415 ( Main/Exploration ); précédent : 000414; suivant : 000416Reproductive isolation and hybridization in sympatric populations of three Dactylorhiza species (Orchidaceae) with different ploidy levels
Auteurs : Koen De Hert [Belgique] ; Hans Jacquemyn [Belgique] ; Sabine Van Glabeke [Belgique] ; Isabel Roldán-Ruiz [Belgique] ; Katrien Vandepitte [Belgique] ; Leen Leus [Belgique] ; Olivier Honnay [Belgique]Source :
- Annals of Botany [ 0305-7364 ] ; 2011.
Abstract
The potential for gene exchange between species with different ploidy levels has long been recognized, but only a few studies have tested this hypothesis
To compare the strength of reproductive barriers between diploid species, and between diploid and tetraploid species, crossing experiments were combined with morphometric and molecular analyses using amplified fragment length polymorphism markers, whereas flow cytometric analyses were used to verify the hybrid origin of putative hybrids.
In both sites, extensive hybridization was observed, indicating that gene flow between species is possible within the investigated populations. Bayesian assignment analyses indicated that the majority of hybrids were
Strong post-mating reproductive barriers prevent local breakdown of species boundaries in
Url:
DOI: 10.1093/aob/mcr305
PubMed: 22186278
PubMed Central: 3286275
Affiliations:
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Reproductive isolation and hybridization in sympatric populations of three <italic>Dactylorhiza</italic>
species (Orchidaceae) with different ploidy levels</title>
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</affiliation>
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<author><name sortKey="Roldan Ruiz, Isabel" sort="Roldan Ruiz, Isabel" uniqKey="Roldan Ruiz I" first="Isabel" last="Roldán-Ruiz">Isabel Roldán-Ruiz</name>
<affiliation wicri:level="1"><nlm:aff id="af2"><addr-line>Institute for Agricultural and Fisheries Research (ILVO), Plant Sciences Unit, Caritasstraat 21, B-9090 Melle, Belgium</addr-line>
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<author><name sortKey="Vandepitte, Katrien" sort="Vandepitte, Katrien" uniqKey="Vandepitte K" first="Katrien" last="Vandepitte">Katrien Vandepitte</name>
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<author><name sortKey="Leus, Leen" sort="Leus, Leen" uniqKey="Leus L" first="Leen" last="Leus">Leen Leus</name>
<affiliation wicri:level="1"><nlm:aff id="af2"><addr-line>Institute for Agricultural and Fisheries Research (ILVO), Plant Sciences Unit, Caritasstraat 21, B-9090 Melle, Belgium</addr-line>
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</affiliation>
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<author><name sortKey="Honnay, Olivier" sort="Honnay, Olivier" uniqKey="Honnay O" first="Olivier" last="Honnay">Olivier Honnay</name>
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<series><title level="j">Annals of Botany</title>
<idno type="ISSN">0305-7364</idno>
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<imprint><date when="2011">2011</date>
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<front><div type="abstract" xml:lang="en"><sec><title>Background and Aims</title>
<p>The potential for gene exchange between species with different ploidy levels has long been recognized, but only a few studies have tested this hypothesis <italic>in situ</italic>
and most of them focused on not more than two co-occurring species. In this study, we examined hybridization patterns in two sites containing three species of the genus <italic>Dactylorhiza</italic>
(diploid <italic>D. incarnata</italic>
and <italic>D. fuchsii</italic>
and their allotetraploid derivative <italic>D. praetermissa</italic>
).</p>
</sec>
<sec><title>Methods</title>
<p>To compare the strength of reproductive barriers between diploid species, and between diploid and tetraploid species, crossing experiments were combined with morphometric and molecular analyses using amplified fragment length polymorphism markers, whereas flow cytometric analyses were used to verify the hybrid origin of putative hybrids.</p>
</sec>
<sec><title>Key Results</title>
<p>In both sites, extensive hybridization was observed, indicating that gene flow between species is possible within the investigated populations. Bayesian assignment analyses indicated that the majority of hybrids were <italic>F</italic>
<sub>1</sub>
hybrids, but in some cases triple hybrids (hybrids with three species as parents) were observed, suggesting secondary gene flow. Crossing experiments showed that only crosses between pure species yielded a high percentage of viable seeds. When hybrids were involved as either pollen-receptor or pollen-donor, almost no viable seeds were formed, indicating strong post-zygotic reproductive isolation and high sterility.</p>
</sec>
<sec><title>Conclusions</title>
<p>Strong post-mating reproductive barriers prevent local breakdown of species boundaries in <italic>Dactylorhiza</italic>
despite frequent hybridization between parental species. However, the presence of triple hybrids indicates that in some cases hybridization may extend the <italic>F</italic>
<sub>1</sub>
generation.</p>
</sec>
</div>
</front>
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<affiliations><list><country><li>Belgique</li>
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</list>
<tree><country name="Belgique"><noRegion><name sortKey="De Hert, Koen" sort="De Hert, Koen" uniqKey="De Hert K" first="Koen" last="De Hert">Koen De Hert</name>
</noRegion>
<name sortKey="Honnay, Olivier" sort="Honnay, Olivier" uniqKey="Honnay O" first="Olivier" last="Honnay">Olivier Honnay</name>
<name sortKey="Jacquemyn, Hans" sort="Jacquemyn, Hans" uniqKey="Jacquemyn H" first="Hans" last="Jacquemyn">Hans Jacquemyn</name>
<name sortKey="Leus, Leen" sort="Leus, Leen" uniqKey="Leus L" first="Leen" last="Leus">Leen Leus</name>
<name sortKey="Roldan Ruiz, Isabel" sort="Roldan Ruiz, Isabel" uniqKey="Roldan Ruiz I" first="Isabel" last="Roldán-Ruiz">Isabel Roldán-Ruiz</name>
<name sortKey="Van Glabeke, Sabine" sort="Van Glabeke, Sabine" uniqKey="Van Glabeke S" first="Sabine" last="Van Glabeke">Sabine Van Glabeke</name>
<name sortKey="Vandepitte, Katrien" sort="Vandepitte, Katrien" uniqKey="Vandepitte K" first="Katrien" last="Vandepitte">Katrien Vandepitte</name>
</country>
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</record>
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