Metabolomic-Based Study of the Leafy Gall, the Ecological Niche of the Phytopathogen Rhodococcus Fascians, as a Potential Source of Bioactive Compounds
Identifieur interne : 000152 ( Main/Exploration ); précédent : 000151; suivant : 000153Metabolomic-Based Study of the Leafy Gall, the Ecological Niche of the Phytopathogen Rhodococcus Fascians, as a Potential Source of Bioactive Compounds
Auteurs : Aminata P. Nacoulma ; Olivier M. Vandeputte ; Manuella De Lorenzi ; Mondher El Jaziri ; Pierre DuezSource :
- International Journal of Molecular Sciences [ 1422-0067 ] ; 2013.
Abstract
Leafy gall is a plant hyperplasia induced upon
Url:
DOI: 10.3390/ijms140612533
PubMed: 23771021
PubMed Central: 3709798
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Metabolomic-Based Study of the Leafy Gall, the Ecological Niche of the Phytopathogen <italic>Rhodococcus Fascians</italic>, as a Potential Source of Bioactive Compounds</title><author><name sortKey="Nacoulma, Aminata P" sort="Nacoulma, Aminata P" uniqKey="Nacoulma A" first="Aminata P." last="Nacoulma">Aminata P. Nacoulma</name><affiliation><nlm:aff id="af1-ijms-14-12533">Laboratory of Toxicology, Faculty of Pharmacy, Université Libre de Bruxelles, CP 205/1, Boulevard du Triomphe, Brussels B-1050, Belgium; E-Mail:<email>manuela.de.lorenzi@ulb.ac.be</email></nlm:aff></affiliation></author><author><name sortKey="Vandeputte, Olivier M" sort="Vandeputte, Olivier M" uniqKey="Vandeputte O" first="Olivier M." last="Vandeputte">Olivier M. Vandeputte</name><affiliation><nlm:aff id="af2-ijms-14-12533">Laboratory of Plant Biotechnology, Faculty of Sciences, Université Libre de Bruxelles, 12 rue des Professeurs Jeener et Brachet, Gosselies B-6041, Belgium; E-Mails:<email>olivier.vandeputte@ulb.ac.be</email> (O.M.V.);<email>jaziri@ulb.ac.be</email> (M.E.J.)</nlm:aff></affiliation></author><author><name sortKey="De Lorenzi, Manuella" sort="De Lorenzi, Manuella" uniqKey="De Lorenzi M" first="Manuella" last="De Lorenzi">Manuella De Lorenzi</name><affiliation><nlm:aff id="af1-ijms-14-12533">Laboratory of Toxicology, Faculty of Pharmacy, Université Libre de Bruxelles, CP 205/1, Boulevard du Triomphe, Brussels B-1050, Belgium; E-Mail:<email>manuela.de.lorenzi@ulb.ac.be</email></nlm:aff></affiliation></author><author><name sortKey="El Jaziri, Mondher" sort="El Jaziri, Mondher" uniqKey="El Jaziri M" first="Mondher" last="El Jaziri">Mondher El Jaziri</name><affiliation><nlm:aff id="af2-ijms-14-12533">Laboratory of Plant Biotechnology, Faculty of Sciences, Université Libre de Bruxelles, 12 rue des Professeurs Jeener et Brachet, Gosselies B-6041, Belgium; E-Mails:<email>olivier.vandeputte@ulb.ac.be</email> (O.M.V.);<email>jaziri@ulb.ac.be</email> (M.E.J.)</nlm:aff></affiliation></author><author><name sortKey="Duez, Pierre" sort="Duez, Pierre" uniqKey="Duez P" first="Pierre" last="Duez">Pierre Duez</name><affiliation><nlm:aff id="af3-ijms-14-12533">Laboratory of Pharmacognosy, Bromatology and Human Nutrition, Faculty of Pharmacy, Université Libre de Bruxelles, CP 205/9, Boulevard du Triomphe, Brussels B-1050, Belgium; E-Mail:<email>pduez@ulb.ac.be</email></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">23771021</idno><idno type="pmc">3709798</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3709798</idno><idno type="RBID">PMC:3709798</idno><idno type="doi">10.3390/ijms140612533</idno><date when="2013">2013</date><idno type="wicri:Area/Pmc/Corpus">000185</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000185</idno><idno type="wicri:Area/Pmc/Curation">000185</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000185</idno><idno type="wicri:Area/Pmc/Checkpoint">000134</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000134</idno><idno type="wicri:Area/Ncbi/Merge">00167</idno><idno type="wicri:Area/Ncbi/Curation">000167</idno><idno type="wicri:Area/Ncbi/Checkpoint">000167</idno><idno type="wicri:Area/Main/Merge">000152</idno><idno type="wicri:Area/Main/Curation">000152</idno><idno type="wicri:Area/Main/Exploration">000152</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Metabolomic-Based Study of the Leafy Gall, the Ecological Niche of the Phytopathogen <italic>Rhodococcus Fascians</italic>, as a Potential Source of Bioactive Compounds</title><author><name sortKey="Nacoulma, Aminata P" sort="Nacoulma, Aminata P" uniqKey="Nacoulma A" first="Aminata P." last="Nacoulma">Aminata P. Nacoulma</name><affiliation><nlm:aff id="af1-ijms-14-12533">Laboratory of Toxicology, Faculty of Pharmacy, Université Libre de Bruxelles, CP 205/1, Boulevard du Triomphe, Brussels B-1050, Belgium; E-Mail:<email>manuela.de.lorenzi@ulb.ac.be</email></nlm:aff></affiliation></author><author><name sortKey="Vandeputte, Olivier M" sort="Vandeputte, Olivier M" uniqKey="Vandeputte O" first="Olivier M." last="Vandeputte">Olivier M. Vandeputte</name><affiliation><nlm:aff id="af2-ijms-14-12533">Laboratory of Plant Biotechnology, Faculty of Sciences, Université Libre de Bruxelles, 12 rue des Professeurs Jeener et Brachet, Gosselies B-6041, Belgium; E-Mails:<email>olivier.vandeputte@ulb.ac.be</email> (O.M.V.);<email>jaziri@ulb.ac.be</email> (M.E.J.)</nlm:aff></affiliation></author><author><name sortKey="De Lorenzi, Manuella" sort="De Lorenzi, Manuella" uniqKey="De Lorenzi M" first="Manuella" last="De Lorenzi">Manuella De Lorenzi</name><affiliation><nlm:aff id="af1-ijms-14-12533">Laboratory of Toxicology, Faculty of Pharmacy, Université Libre de Bruxelles, CP 205/1, Boulevard du Triomphe, Brussels B-1050, Belgium; E-Mail:<email>manuela.de.lorenzi@ulb.ac.be</email></nlm:aff></affiliation></author><author><name sortKey="El Jaziri, Mondher" sort="El Jaziri, Mondher" uniqKey="El Jaziri M" first="Mondher" last="El Jaziri">Mondher El Jaziri</name><affiliation><nlm:aff id="af2-ijms-14-12533">Laboratory of Plant Biotechnology, Faculty of Sciences, Université Libre de Bruxelles, 12 rue des Professeurs Jeener et Brachet, Gosselies B-6041, Belgium; E-Mails:<email>olivier.vandeputte@ulb.ac.be</email> (O.M.V.);<email>jaziri@ulb.ac.be</email> (M.E.J.)</nlm:aff></affiliation></author><author><name sortKey="Duez, Pierre" sort="Duez, Pierre" uniqKey="Duez P" first="Pierre" last="Duez">Pierre Duez</name><affiliation><nlm:aff id="af3-ijms-14-12533">Laboratory of Pharmacognosy, Bromatology and Human Nutrition, Faculty of Pharmacy, Université Libre de Bruxelles, CP 205/9, Boulevard du Triomphe, Brussels B-1050, Belgium; E-Mail:<email>pduez@ulb.ac.be</email></nlm:aff></affiliation></author></analytic><series><title level="j">International Journal of Molecular Sciences</title><idno type="eISSN">1422-0067</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Leafy gall is a plant hyperplasia induced upon <italic>Rhodococcus fascians</italic> infection. Previously, by genomic and transcriptomic analysis, it has been reported that, at the early stage of symptom development, both primary and secondary metabolisms are modified. The present study is based on the hypothesis that fully developed leafy gall, could represent a potential source of new bioactive compounds. Therefore, non-targeted metabolomic analysis of aqueous and chloroform extracts of leafy gall and non-infected tobacco was carried out by <sup>1</sup>H-NMR coupled to principal component analysis (PCA) and orthogonal projections to latent structures-discriminant analysis (OPLS-DA). Polar metabolite profiling reflects modifications mainly in the primary metabolites and in some polyphenolics. In contrast, main modifications occurring in non-polar metabolites concern secondary metabolites, and gas chromatography and mass spectrometry (GC-MS) evidenced alterations in diterpenoids family. Analysis of crude extracts of leafy galls and non-infected tobacco leaves exhibited a distinct antiproliferative activity against all four tested human cancer cell lines. A bio-guided fractionation of chloroformic crude extract yield to semi-purified fractions, which inhibited proliferation of glioblastoma U373 cells with IC<sub>50</sub> between 14.0 and 2.4 μg/mL. Discussion is focused on the consequence of these metabolic changes, with respect to plant defense mechanisms following infection. Considering the promising role of diterpenoid family as bioactive compounds, leafy gall may rather be a propitious source for drug discovery.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Agrios, G N" uniqKey="Agrios G">G.N. Agrios</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hayward, A" uniqKey="Hayward A">A. Hayward</name></author><author><name sortKey="Stone, G" uniqKey="Stone G">G. 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Bury</name></author><author><name sortKey="Girault, A" uniqKey="Girault A">A Girault</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list></list><tree><noCountry><name sortKey="De Lorenzi, Manuella" sort="De Lorenzi, Manuella" uniqKey="De Lorenzi M" first="Manuella" last="De Lorenzi">Manuella De Lorenzi</name><name sortKey="Duez, Pierre" sort="Duez, Pierre" uniqKey="Duez P" first="Pierre" last="Duez">Pierre Duez</name><name sortKey="El Jaziri, Mondher" sort="El Jaziri, Mondher" uniqKey="El Jaziri M" first="Mondher" last="El Jaziri">Mondher El Jaziri</name><name sortKey="Nacoulma, Aminata P" sort="Nacoulma, Aminata P" uniqKey="Nacoulma A" first="Aminata P." last="Nacoulma">Aminata P. Nacoulma</name><name sortKey="Vandeputte, Olivier M" sort="Vandeputte, Olivier M" uniqKey="Vandeputte O" first="Olivier M." last="Vandeputte">Olivier M. Vandeputte</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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