Nanocarrier Mediated siRNA Delivery Targeting Stem Cell Differentiation.
Identifieur interne : 000057 ( Main/Exploration ); précédent : 000056; suivant : 000058Nanocarrier Mediated siRNA Delivery Targeting Stem Cell Differentiation.
Auteurs : Fiona Fernandes [Inde] ; Pooja Kotharkar [Inde] ; Adrija Chakravorty [Inde] ; Meenal Kowshik [Inde] ; Indrani Talukdar [Inde]Source :
- Current stem cell research & therapy [ 2212-3946 ] ; 2020.
Abstract
Stem cell-based regenerative medicine holds exceptional therapeutic potential and hence the development of efficient techniques to enhance control over the rate of differentiation has been the focus of active research. One of the strategies to achieve this involves delivering siRNA into stem cells and exploiting the RNA interference (RNAi) mechanism. Transport of siRNA across the cell membrane is a challenge due to its anionic property, especially in primary human cells and stem cells. Moreover, naked siRNA incites immune responses, may cause off-target effects, exhibits low stability and is easily degraded by endonucleases in the bloodstream. Although siRNA delivery using viral vectors and electroporation has been used in stem cells, these methods demonstrate low transfection efficiency, cytotoxicity, immunogenicity, events of integration and may involve laborious customization. With the advent of nanotechnology, nanocarriers which act as novel gene delivery vehicles designed to overcome the problems associated with safety and practicality are being developed. The various nanomaterials that are currently being explored and discussed in this review include liposomes, carbon nanotubes, quantum dots, protein and peptide nanocarriers, magnetic nanoparticles, polymeric nanoparticles, etc. These nanodelivery agents exhibit advantages such as low immunogenic response, biocompatibility, design flexibility allowing for surface modification and functionalization, and control over the surface topography for achieving the desired rate of siRNA delivery and improved gene knockdown efficiency. This review also includes discussion on siRNA co-delivery with imaging agents, plasmid DNA, drugs etc. to achieve combined diagnostic and enhanced therapeutic functionality, both for in vitro and in vivo applications.
DOI: 10.2174/1574888X14666191202095041
PubMed: 31789134
Affiliations:
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K. Birla Goa campus, Zuarinagar, Goa-403726, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biological Sciences, BITS Pilani, K. K. Birla Goa campus, Zuarinagar, Goa-403726</wicri:regionArea><wicri:noRegion>Goa-403726</wicri:noRegion></affiliation></author><author><name sortKey="Kotharkar, Pooja" sort="Kotharkar, Pooja" uniqKey="Kotharkar P" first="Pooja" last="Kotharkar">Pooja Kotharkar</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biological Sciences, BITS Pilani, K. K. Birla Goa campus, Zuarinagar, Goa-403726, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biological Sciences, BITS Pilani, K. K. Birla Goa campus, Zuarinagar, Goa-403726</wicri:regionArea><wicri:noRegion>Goa-403726</wicri:noRegion></affiliation></author><author><name sortKey="Chakravorty, Adrija" sort="Chakravorty, Adrija" uniqKey="Chakravorty A" first="Adrija" last="Chakravorty">Adrija Chakravorty</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biological Sciences, BITS Pilani, K. K. Birla Goa campus, Zuarinagar, Goa-403726, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biological Sciences, BITS Pilani, K. K. Birla Goa campus, Zuarinagar, Goa-403726</wicri:regionArea><wicri:noRegion>Goa-403726</wicri:noRegion></affiliation></author><author><name sortKey="Kowshik, Meenal" sort="Kowshik, Meenal" uniqKey="Kowshik M" first="Meenal" last="Kowshik">Meenal Kowshik</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biological Sciences, BITS Pilani, K. K. Birla Goa campus, Zuarinagar, Goa-403726, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biological Sciences, BITS Pilani, K. K. Birla Goa campus, Zuarinagar, Goa-403726</wicri:regionArea><wicri:noRegion>Goa-403726</wicri:noRegion></affiliation></author><author><name sortKey="Talukdar, Indrani" sort="Talukdar, Indrani" uniqKey="Talukdar I" first="Indrani" last="Talukdar">Indrani Talukdar</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biological Sciences, BITS Pilani, K. K. Birla Goa campus, Zuarinagar, Goa-403726, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biological Sciences, BITS Pilani, K. K. Birla Goa campus, Zuarinagar, Goa-403726</wicri:regionArea><wicri:noRegion>Goa-403726</wicri:noRegion></affiliation></author></analytic><series><title level="j">Current stem cell research & therapy</title><idno type="eISSN">2212-3946</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Stem cell-based regenerative medicine holds exceptional therapeutic potential and hence the development of efficient techniques to enhance control over the rate of differentiation has been the focus of active research. One of the strategies to achieve this involves delivering siRNA into stem cells and exploiting the RNA interference (RNAi) mechanism. Transport of siRNA across the cell membrane is a challenge due to its anionic property, especially in primary human cells and stem cells. Moreover, naked siRNA incites immune responses, may cause off-target effects, exhibits low stability and is easily degraded by endonucleases in the bloodstream. Although siRNA delivery using viral vectors and electroporation has been used in stem cells, these methods demonstrate low transfection efficiency, cytotoxicity, immunogenicity, events of integration and may involve laborious customization. With the advent of nanotechnology, nanocarriers which act as novel gene delivery vehicles designed to overcome the problems associated with safety and practicality are being developed. The various nanomaterials that are currently being explored and discussed in this review include liposomes, carbon nanotubes, quantum dots, protein and peptide nanocarriers, magnetic nanoparticles, polymeric nanoparticles, etc. These nanodelivery agents exhibit advantages such as low immunogenic response, biocompatibility, design flexibility allowing for surface modification and functionalization, and control over the surface topography for achieving the desired rate of siRNA delivery and improved gene knockdown efficiency. This review also includes discussion on siRNA co-delivery with imaging agents, plasmid DNA, drugs etc. to achieve combined diagnostic and enhanced therapeutic functionality, both for in vitro and in vivo applications.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">31789134</PMID><DateRevised><Year>2020</Year><Month>04</Month><Day>02</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">2212-3946</ISSN><JournalIssue CitedMedium="Internet"><Volume>15</Volume><Issue>2</Issue><PubDate><Year>2020</Year></PubDate></JournalIssue><Title>Current stem cell research & therapy</Title><ISOAbbreviation>Curr Stem Cell Res Ther</ISOAbbreviation></Journal><ArticleTitle>Nanocarrier Mediated siRNA Delivery Targeting Stem Cell Differentiation.</ArticleTitle><Pagination><MedlinePgn>155-172</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.2174/1574888X14666191202095041</ELocationID><Abstract><AbstractText>Stem cell-based regenerative medicine holds exceptional therapeutic potential and hence the development of efficient techniques to enhance control over the rate of differentiation has been the focus of active research. One of the strategies to achieve this involves delivering siRNA into stem cells and exploiting the RNA interference (RNAi) mechanism. Transport of siRNA across the cell membrane is a challenge due to its anionic property, especially in primary human cells and stem cells. Moreover, naked siRNA incites immune responses, may cause off-target effects, exhibits low stability and is easily degraded by endonucleases in the bloodstream. Although siRNA delivery using viral vectors and electroporation has been used in stem cells, these methods demonstrate low transfection efficiency, cytotoxicity, immunogenicity, events of integration and may involve laborious customization. With the advent of nanotechnology, nanocarriers which act as novel gene delivery vehicles designed to overcome the problems associated with safety and practicality are being developed. The various nanomaterials that are currently being explored and discussed in this review include liposomes, carbon nanotubes, quantum dots, protein and peptide nanocarriers, magnetic nanoparticles, polymeric nanoparticles, etc. These nanodelivery agents exhibit advantages such as low immunogenic response, biocompatibility, design flexibility allowing for surface modification and functionalization, and control over the surface topography for achieving the desired rate of siRNA delivery and improved gene knockdown efficiency. This review also includes discussion on siRNA co-delivery with imaging agents, plasmid DNA, drugs etc. to achieve combined diagnostic and enhanced therapeutic functionality, both for in vitro and in vivo applications.</AbstractText><CopyrightInformation>Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Fernandes</LastName><ForeName>Fiona</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Department of Biological Sciences, BITS Pilani, K. K. Birla Goa campus, Zuarinagar, Goa-403726, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kotharkar</LastName><ForeName>Pooja</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Department of Biological Sciences, BITS Pilani, K. K. Birla Goa campus, Zuarinagar, Goa-403726, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chakravorty</LastName><ForeName>Adrija</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Biological Sciences, BITS Pilani, K. K. Birla Goa campus, Zuarinagar, Goa-403726, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kowshik</LastName><ForeName>Meenal</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Biological Sciences, BITS Pilani, K. K. Birla Goa campus, Zuarinagar, Goa-403726, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Talukdar</LastName><ForeName>Indrani</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>Department of Biological Sciences, BITS Pilani, K. K. Birla Goa campus, Zuarinagar, Goa-403726, India.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United Arab Emirates</Country><MedlineTA>Curr Stem Cell Res Ther</MedlineTA><NlmUniqueID>101272517</NlmUniqueID><ISSNLinking>1574-888X</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Stem cell</Keyword><Keyword MajorTopicYN="N">differentiation</Keyword><Keyword MajorTopicYN="N">nanocarriers</Keyword><Keyword MajorTopicYN="N">nanoparticles</Keyword><Keyword MajorTopicYN="N">regenerative medicine</Keyword><Keyword MajorTopicYN="N">siRNA</Keyword><Keyword MajorTopicYN="N">transfection.</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>08</Month><Day>06</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>09</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>11</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>12</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>12</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>12</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31789134</ArticleId><ArticleId IdType="pii">CSCR-EPUB-102679</ArticleId><ArticleId IdType="doi">10.2174/1574888X14666191202095041</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Inde</li></country></list><tree><country name="Inde"><noRegion><name sortKey="Fernandes, Fiona" sort="Fernandes, Fiona" uniqKey="Fernandes F" first="Fiona" last="Fernandes">Fiona Fernandes</name></noRegion><name sortKey="Chakravorty, Adrija" sort="Chakravorty, Adrija" uniqKey="Chakravorty A" first="Adrija" last="Chakravorty">Adrija Chakravorty</name><name sortKey="Kotharkar, Pooja" sort="Kotharkar, Pooja" uniqKey="Kotharkar P" first="Pooja" last="Kotharkar">Pooja Kotharkar</name><name sortKey="Kowshik, Meenal" sort="Kowshik, Meenal" uniqKey="Kowshik M" first="Meenal" last="Kowshik">Meenal Kowshik</name><name sortKey="Talukdar, Indrani" sort="Talukdar, Indrani" uniqKey="Talukdar I" first="Indrani" last="Talukdar">Indrani Talukdar</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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