Flavopereirine-An Alkaloid Derived from Geissospermum vellosii-Presents Leishmanicidal Activity In Vitro.
Identifieur interne : 000468 ( Main/Corpus ); précédent : 000467; suivant : 000469Flavopereirine-An Alkaloid Derived from Geissospermum vellosii-Presents Leishmanicidal Activity In Vitro.
Auteurs : João Victor Da Silva E Silva ; Heliton Patrick Cordovil Brigido ; Kelly Cristina Oliveira De Albuquerque ; Josiwander Miranda Carvalho ; Jordano Ferreira Reis ; Lara Vinhal Faria ; Márlia Coelho-Ferreira ; Fernando Tobias Silveira ; Agnaldo Da Silva Carneiro ; Sandro Percário ; Andrey Moacir Do Rosário Marinho ; Maria Fâni DolabelaSource :
- Molecules (Basel, Switzerland) [ 1420-3049 ] ; 2019.
English descriptors
- KwdEn :
- Antipain (chemistry), Antipain (pharmacology), Antiprotozoal Agents (chemistry), Antiprotozoal Agents (isolation & purification), Antiprotozoal Agents (pharmacology), Apocynaceae (chemistry), Carbolines (chemistry), Carbolines (isolation & purification), Carbolines (pharmacology), Cell Survival (drug effects), Humans (MeSH), Indole Alkaloids (chemistry), Indole Alkaloids (classification), Indole Alkaloids (isolation & purification), Inhibitory Concentration 50 (MeSH), Leishmania mexicana (drug effects), Leishmania mexicana (growth & development), Life Cycle Stages (drug effects), Life Cycle Stages (physiology), Molecular Docking Simulation (MeSH), Molecular Dynamics Simulation (MeSH), Plant Bark (chemistry), Plant Extracts (chemistry), Protozoan Proteins (antagonists & inhibitors), Protozoan Proteins (chemistry), Serine Endopeptidases (chemistry), THP-1 Cells (MeSH).
- MESH :
- chemical , antagonists & inhibitors : Protozoan Proteins.
- chemical , chemistry : Antipain, Antiprotozoal Agents, Carbolines, Indole Alkaloids, Plant Extracts, Protozoan Proteins, Serine Endopeptidases.
- chemical , classification : Indole Alkaloids.
- chemical , isolation & purification : Antiprotozoal Agents, Carbolines, Indole Alkaloids.
- chemical , pharmacology : Antipain, Antiprotozoal Agents, Carbolines.
- chemistry : Apocynaceae, Plant Bark.
- drug effects : Cell Survival, Leishmania mexicana, Life Cycle Stages.
- growth & development : Leishmania mexicana.
- physiology : Life Cycle Stages.
- Humans, Inhibitory Concentration 50, Molecular Docking Simulation, Molecular Dynamics Simulation, THP-1 Cells.
Abstract
Chemotherapy is limited in the treatment of leishmaniasis due to the toxic effects of drugs, low efficacy of alternative treatments, and resistance of the parasite. This work assesses the in vitro activity of flavopereirine on promastigote cultures of Leishmania amazonensis. In addition, an in silico evaluation of the physicochemical characteristics of this alkaloid is performed. The extract and fractions were characterized by thin-layer chromatography and HPLC-DAD, yielding an alkaloid identified by NMR. The antileishmanial activity and cytotoxicity were assayed by cell viability test (MTT). The theoretical molecular properties were calculated on the Molinspiration website. The fractionation made it possible to isolate a beta-carboline alkaloid (flavopereirine) in the alkaloid fraction. Moreover, it led to obtaining a fraction with greater antileishmanial activity, since flavopereirine is very active. Regarding the exposure time, a greater inhibitory effect of flavopereirine was observed at 24 h and 72 h (IC50 of 0.23 and 0.15 μg/mL, respectively). The extract, fractions, and flavopereirine presented low toxicity, with high selectivity for the alkaloid. Furthermore, flavopereirine showed no violation of Lipinski's rule of five, showing even better results than the known inhibitor of oligopeptidase B, antipain, with three violations. Flavopereirine also interacted with residue Tyr-499 of oligopeptidase B during the molecular dynamics simulations, giving a few insights of a possible favorable mechanism of interaction and a possible inhibitory pathway. Flavopereirine proved to be a promising molecule for its antileishmanial activity.
DOI: 10.3390/molecules24040785
PubMed: 30795632
PubMed Central: PMC6412932
Links to Exploration step
pubmed:30795632Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Flavopereirine-An Alkaloid Derived from <i>Geissospermum vellosii</i>-Presents Leishmanicidal Activity In Vitro.</title><author><name sortKey="Da Silva E Silva, Joao Victor" sort="Da Silva E Silva, Joao Victor" uniqKey="Da Silva E Silva J" first="João Victor" last="Da Silva E Silva">João Victor Da Silva E Silva</name><affiliation><nlm:affiliation>Post-Graduate Program in Pharmaceutical Sciences, Federal University of Pará, Belém, 66075-110 PA, Brazil. jvssilva89@gmail.com.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Laboratory of Immunomodulation and Protozoology, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro 21040-900, Brazil. jvssilva89@gmail.com.</nlm:affiliation></affiliation></author><author><name sortKey="Cordovil Brigido, Heliton Patrick" sort="Cordovil Brigido, Heliton Patrick" uniqKey="Cordovil Brigido H" first="Heliton Patrick" last="Cordovil Brigido">Heliton Patrick Cordovil Brigido</name><affiliation><nlm:affiliation>Post-Graduate Program in Pharmaceutical Innovation, Federal University of Pará, Belém, 66075-110 PA, Brazil. helitom2009@hotmail.com.</nlm:affiliation></affiliation></author><author><name sortKey="Oliveira De Albuquerque, Kelly Cristina" sort="Oliveira De Albuquerque, Kelly Cristina" uniqKey="Oliveira De Albuquerque K" first="Kelly Cristina" last="Oliveira De Albuquerque">Kelly Cristina Oliveira De Albuquerque</name><affiliation><nlm:affiliation>Post-Graduate Program in Pharmaceutical Sciences, Federal University of Pará, Belém, 66075-110 PA, Brazil. kellyoalbuquerque@hotmail.com.</nlm:affiliation></affiliation></author><author><name sortKey="Miranda Carvalho, Josiwander" sort="Miranda Carvalho, Josiwander" uniqKey="Miranda Carvalho J" first="Josiwander" last="Miranda Carvalho">Josiwander Miranda Carvalho</name><affiliation><nlm:affiliation>College of Chemistry, Federal University of Pará, Belém, 66075-110 PA, Brazil. mcwander@hotmail.com.</nlm:affiliation></affiliation></author><author><name sortKey="Ferreira Reis, Jordano" sort="Ferreira Reis, Jordano" uniqKey="Ferreira Reis J" first="Jordano" last="Ferreira Reis">Jordano Ferreira Reis</name><affiliation><nlm:affiliation>Faculty of Pharmacy, Federal University of Pará, Belém, 66075-110 PA, Brazil. jordanoreis@outlook.com.</nlm:affiliation></affiliation></author><author><name sortKey="Vinhal Faria, Lara" sort="Vinhal Faria, Lara" uniqKey="Vinhal Faria L" first="Lara" last="Vinhal Faria">Lara Vinhal Faria</name><affiliation><nlm:affiliation>Faculty of Pharmacy, Federal University of Pará, Belém, 66075-110 PA, Brazil. laravf24@gmail.com.</nlm:affiliation></affiliation></author><author><name sortKey="Coelho Ferreira, Marlia" sort="Coelho Ferreira, Marlia" uniqKey="Coelho Ferreira M" first="Márlia" last="Coelho-Ferreira">Márlia Coelho-Ferreira</name><affiliation><nlm:affiliation>Emílio Goeldi Paraense Museum, Coordination of Botany, Ministry of Science, Technology, Innovation and Communications. Belém, 66077-530 PA, Brazil. mcoelho@museu-goeldi.br.</nlm:affiliation></affiliation></author><author><name sortKey="Silveira, Fernando Tobias" sort="Silveira, Fernando Tobias" uniqKey="Silveira F" first="Fernando Tobias" last="Silveira">Fernando Tobias Silveira</name><affiliation><nlm:affiliation>Evandro Chagas Institute, National Health Foundation, BR-316 Highway km 7, Ananindeua, 67030-000 PA, Brazil. fernandotobias@iec.pa.gov.br.</nlm:affiliation></affiliation></author><author><name sortKey="Da Silva Carneiro, Agnaldo" sort="Da Silva Carneiro, Agnaldo" uniqKey="Da Silva Carneiro A" first="Agnaldo" last="Da Silva Carneiro">Agnaldo Da Silva Carneiro</name><affiliation><nlm:affiliation>Faculty of Pharmacy, Federal University of Pará, Belém, 66075-110 PA, Brazil. agsicar@yahoo.com.br.</nlm:affiliation></affiliation></author><author><name sortKey="Percario, Sandro" sort="Percario, Sandro" uniqKey="Percario S" first="Sandro" last="Percário">Sandro Percário</name><affiliation><nlm:affiliation>US Centers for Disease Control and Prevention (CDC), Atlanta, 30329 GA, USA. percario@ufpa.br.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Oxidative Stress Research Lab, Institute of Biological Sciences (ICB), Federal University of Pará, Belém, 66075-110 PA, Brazil. percario@ufpa.br.</nlm:affiliation></affiliation></author><author><name sortKey="Do Rosario Marinho, Andrey Moacir" sort="Do Rosario Marinho, Andrey Moacir" uniqKey="Do Rosario Marinho A" first="Andrey Moacir" last="Do Rosário Marinho">Andrey Moacir Do Rosário Marinho</name><affiliation><nlm:affiliation>College of Chemistry, Federal University of Pará, Belém, 66075-110 PA, Brazil. andrey@ufpa.br.</nlm:affiliation></affiliation></author><author><name sortKey="Dolabela, Maria Fani" sort="Dolabela, Maria Fani" uniqKey="Dolabela M" first="Maria Fâni" last="Dolabela">Maria Fâni Dolabela</name><affiliation><nlm:affiliation>Post-Graduate Program in Pharmaceutical Sciences, Federal University of Pará, Belém, 66075-110 PA, Brazil. fanidolabela20@gmail.com.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Post-Graduate Program in Pharmaceutical Innovation, Federal University of Pará, Belém, 66075-110 PA, Brazil. fanidolabela20@gmail.com.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:30795632</idno><idno type="pmid">30795632</idno><idno type="doi">10.3390/molecules24040785</idno><idno type="pmc">PMC6412932</idno><idno type="wicri:Area/Main/Corpus">000468</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000468</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Flavopereirine-An Alkaloid Derived from <i>Geissospermum vellosii</i>-Presents Leishmanicidal Activity In Vitro.</title><author><name sortKey="Da Silva E Silva, Joao Victor" sort="Da Silva E Silva, Joao Victor" uniqKey="Da Silva E Silva J" first="João Victor" last="Da Silva E Silva">João Victor Da Silva E Silva</name><affiliation><nlm:affiliation>Post-Graduate Program in Pharmaceutical Sciences, Federal University of Pará, Belém, 66075-110 PA, Brazil. jvssilva89@gmail.com.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Laboratory of Immunomodulation and Protozoology, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro 21040-900, Brazil. jvssilva89@gmail.com.</nlm:affiliation></affiliation></author><author><name sortKey="Cordovil Brigido, Heliton Patrick" sort="Cordovil Brigido, Heliton Patrick" uniqKey="Cordovil Brigido H" first="Heliton Patrick" last="Cordovil Brigido">Heliton Patrick Cordovil Brigido</name><affiliation><nlm:affiliation>Post-Graduate Program in Pharmaceutical Innovation, Federal University of Pará, Belém, 66075-110 PA, Brazil. helitom2009@hotmail.com.</nlm:affiliation></affiliation></author><author><name sortKey="Oliveira De Albuquerque, Kelly Cristina" sort="Oliveira De Albuquerque, Kelly Cristina" uniqKey="Oliveira De Albuquerque K" first="Kelly Cristina" last="Oliveira De Albuquerque">Kelly Cristina Oliveira De Albuquerque</name><affiliation><nlm:affiliation>Post-Graduate Program in Pharmaceutical Sciences, Federal University of Pará, Belém, 66075-110 PA, Brazil. kellyoalbuquerque@hotmail.com.</nlm:affiliation></affiliation></author><author><name sortKey="Miranda Carvalho, Josiwander" sort="Miranda Carvalho, Josiwander" uniqKey="Miranda Carvalho J" first="Josiwander" last="Miranda Carvalho">Josiwander Miranda Carvalho</name><affiliation><nlm:affiliation>College of Chemistry, Federal University of Pará, Belém, 66075-110 PA, Brazil. mcwander@hotmail.com.</nlm:affiliation></affiliation></author><author><name sortKey="Ferreira Reis, Jordano" sort="Ferreira Reis, Jordano" uniqKey="Ferreira Reis J" first="Jordano" last="Ferreira Reis">Jordano Ferreira Reis</name><affiliation><nlm:affiliation>Faculty of Pharmacy, Federal University of Pará, Belém, 66075-110 PA, Brazil. jordanoreis@outlook.com.</nlm:affiliation></affiliation></author><author><name sortKey="Vinhal Faria, Lara" sort="Vinhal Faria, Lara" uniqKey="Vinhal Faria L" first="Lara" last="Vinhal Faria">Lara Vinhal Faria</name><affiliation><nlm:affiliation>Faculty of Pharmacy, Federal University of Pará, Belém, 66075-110 PA, Brazil. laravf24@gmail.com.</nlm:affiliation></affiliation></author><author><name sortKey="Coelho Ferreira, Marlia" sort="Coelho Ferreira, Marlia" uniqKey="Coelho Ferreira M" first="Márlia" last="Coelho-Ferreira">Márlia Coelho-Ferreira</name><affiliation><nlm:affiliation>Emílio Goeldi Paraense Museum, Coordination of Botany, Ministry of Science, Technology, Innovation and Communications. Belém, 66077-530 PA, Brazil. mcoelho@museu-goeldi.br.</nlm:affiliation></affiliation></author><author><name sortKey="Silveira, Fernando Tobias" sort="Silveira, Fernando Tobias" uniqKey="Silveira F" first="Fernando Tobias" last="Silveira">Fernando Tobias Silveira</name><affiliation><nlm:affiliation>Evandro Chagas Institute, National Health Foundation, BR-316 Highway km 7, Ananindeua, 67030-000 PA, Brazil. fernandotobias@iec.pa.gov.br.</nlm:affiliation></affiliation></author><author><name sortKey="Da Silva Carneiro, Agnaldo" sort="Da Silva Carneiro, Agnaldo" uniqKey="Da Silva Carneiro A" first="Agnaldo" last="Da Silva Carneiro">Agnaldo Da Silva Carneiro</name><affiliation><nlm:affiliation>Faculty of Pharmacy, Federal University of Pará, Belém, 66075-110 PA, Brazil. agsicar@yahoo.com.br.</nlm:affiliation></affiliation></author><author><name sortKey="Percario, Sandro" sort="Percario, Sandro" uniqKey="Percario S" first="Sandro" last="Percário">Sandro Percário</name><affiliation><nlm:affiliation>US Centers for Disease Control and Prevention (CDC), Atlanta, 30329 GA, USA. percario@ufpa.br.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Oxidative Stress Research Lab, Institute of Biological Sciences (ICB), Federal University of Pará, Belém, 66075-110 PA, Brazil. percario@ufpa.br.</nlm:affiliation></affiliation></author><author><name sortKey="Do Rosario Marinho, Andrey Moacir" sort="Do Rosario Marinho, Andrey Moacir" uniqKey="Do Rosario Marinho A" first="Andrey Moacir" last="Do Rosário Marinho">Andrey Moacir Do Rosário Marinho</name><affiliation><nlm:affiliation>College of Chemistry, Federal University of Pará, Belém, 66075-110 PA, Brazil. andrey@ufpa.br.</nlm:affiliation></affiliation></author><author><name sortKey="Dolabela, Maria Fani" sort="Dolabela, Maria Fani" uniqKey="Dolabela M" first="Maria Fâni" last="Dolabela">Maria Fâni Dolabela</name><affiliation><nlm:affiliation>Post-Graduate Program in Pharmaceutical Sciences, Federal University of Pará, Belém, 66075-110 PA, Brazil. fanidolabela20@gmail.com.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Post-Graduate Program in Pharmaceutical Innovation, Federal University of Pará, Belém, 66075-110 PA, Brazil. fanidolabela20@gmail.com.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Molecules (Basel, Switzerland)</title><idno type="eISSN">1420-3049</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antipain (chemistry)</term><term>Antipain (pharmacology)</term><term>Antiprotozoal Agents (chemistry)</term><term>Antiprotozoal Agents (isolation & purification)</term><term>Antiprotozoal Agents (pharmacology)</term><term>Apocynaceae (chemistry)</term><term>Carbolines (chemistry)</term><term>Carbolines (isolation & purification)</term><term>Carbolines (pharmacology)</term><term>Cell Survival (drug effects)</term><term>Humans (MeSH)</term><term>Indole Alkaloids (chemistry)</term><term>Indole Alkaloids (classification)</term><term>Indole Alkaloids (isolation & purification)</term><term>Inhibitory Concentration 50 (MeSH)</term><term>Leishmania mexicana (drug effects)</term><term>Leishmania mexicana (growth & development)</term><term>Life Cycle Stages (drug effects)</term><term>Life Cycle Stages (physiology)</term><term>Molecular Docking Simulation (MeSH)</term><term>Molecular Dynamics Simulation (MeSH)</term><term>Plant Bark (chemistry)</term><term>Plant Extracts (chemistry)</term><term>Protozoan Proteins (antagonists & inhibitors)</term><term>Protozoan Proteins (chemistry)</term><term>Serine Endopeptidases (chemistry)</term><term>THP-1 Cells (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Protozoan Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Antipain</term><term>Antiprotozoal Agents</term><term>Carbolines</term><term>Indole Alkaloids</term><term>Plant Extracts</term><term>Protozoan Proteins</term><term>Serine Endopeptidases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="classification" xml:lang="en"><term>Indole Alkaloids</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Antiprotozoal Agents</term><term>Carbolines</term><term>Indole Alkaloids</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Antipain</term><term>Antiprotozoal Agents</term><term>Carbolines</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Apocynaceae</term><term>Plant Bark</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cell Survival</term><term>Leishmania mexicana</term><term>Life Cycle Stages</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Leishmania mexicana</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Life Cycle Stages</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Inhibitory Concentration 50</term><term>Molecular Docking Simulation</term><term>Molecular Dynamics Simulation</term><term>THP-1 Cells</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Chemotherapy is limited in the treatment of leishmaniasis due to the toxic effects of drugs, low efficacy of alternative treatments, and resistance of the parasite. This work assesses the in vitro activity of flavopereirine on promastigote cultures of <i>Leishmania amazonensis</i>. In addition, an in silico evaluation of the physicochemical characteristics of this alkaloid is performed. The extract and fractions were characterized by thin-layer chromatography and HPLC-DAD, yielding an alkaloid identified by NMR. The antileishmanial activity and cytotoxicity were assayed by cell viability test (MTT). The theoretical molecular properties were calculated on the Molinspiration website. The fractionation made it possible to isolate a beta-carboline alkaloid (flavopereirine) in the alkaloid fraction. Moreover, it led to obtaining a fraction with greater antileishmanial activity, since flavopereirine is very active. Regarding the exposure time, a greater inhibitory effect of flavopereirine was observed at 24 h and 72 h (IC<sub>50</sub> of 0.23 and 0.15 μg/mL, respectively). The extract, fractions, and flavopereirine presented low toxicity, with high selectivity for the alkaloid. Furthermore, flavopereirine showed no violation of Lipinski's rule of five, showing even better results than the known inhibitor of oligopeptidase B, antipain, with three violations. Flavopereirine also interacted with residue Tyr-499 of oligopeptidase B during the molecular dynamics simulations, giving a few insights of a possible favorable mechanism of interaction and a possible inhibitory pathway. Flavopereirine proved to be a promising molecule for its antileishmanial activity.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30795632</PMID><DateCompleted><Year>2019</Year><Month>06</Month><Day>10</Day></DateCompleted><DateRevised><Year>2020</Year><Month>02</Month><Day>25</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1420-3049</ISSN><JournalIssue CitedMedium="Internet"><Volume>24</Volume><Issue>4</Issue><PubDate><Year>2019</Year><Month>Feb</Month><Day>21</Day></PubDate></JournalIssue><Title>Molecules (Basel, Switzerland)</Title><ISOAbbreviation>Molecules</ISOAbbreviation></Journal><ArticleTitle>Flavopereirine-An Alkaloid Derived from <i>Geissospermum vellosii</i>-Presents Leishmanicidal Activity In Vitro.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E785</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/molecules24040785</ELocationID><Abstract><AbstractText>Chemotherapy is limited in the treatment of leishmaniasis due to the toxic effects of drugs, low efficacy of alternative treatments, and resistance of the parasite. This work assesses the in vitro activity of flavopereirine on promastigote cultures of <i>Leishmania amazonensis</i>. In addition, an in silico evaluation of the physicochemical characteristics of this alkaloid is performed. The extract and fractions were characterized by thin-layer chromatography and HPLC-DAD, yielding an alkaloid identified by NMR. The antileishmanial activity and cytotoxicity were assayed by cell viability test (MTT). The theoretical molecular properties were calculated on the Molinspiration website. The fractionation made it possible to isolate a beta-carboline alkaloid (flavopereirine) in the alkaloid fraction. Moreover, it led to obtaining a fraction with greater antileishmanial activity, since flavopereirine is very active. Regarding the exposure time, a greater inhibitory effect of flavopereirine was observed at 24 h and 72 h (IC<sub>50</sub> of 0.23 and 0.15 μg/mL, respectively). The extract, fractions, and flavopereirine presented low toxicity, with high selectivity for the alkaloid. Furthermore, flavopereirine showed no violation of Lipinski's rule of five, showing even better results than the known inhibitor of oligopeptidase B, antipain, with three violations. Flavopereirine also interacted with residue Tyr-499 of oligopeptidase B during the molecular dynamics simulations, giving a few insights of a possible favorable mechanism of interaction and a possible inhibitory pathway. Flavopereirine proved to be a promising molecule for its antileishmanial activity.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>da Silva E Silva</LastName><ForeName>João Victor</ForeName><Initials>JV</Initials><AffiliationInfo><Affiliation>Post-Graduate Program in Pharmaceutical Sciences, Federal University of Pará, Belém, 66075-110 PA, Brazil. jvssilva89@gmail.com.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Laboratory of Immunomodulation and Protozoology, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro 21040-900, Brazil. jvssilva89@gmail.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cordovil Brigido</LastName><ForeName>Heliton Patrick</ForeName><Initials>HP</Initials><AffiliationInfo><Affiliation>Post-Graduate Program in Pharmaceutical Innovation, Federal University of Pará, Belém, 66075-110 PA, Brazil. helitom2009@hotmail.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Oliveira de Albuquerque</LastName><ForeName>Kelly Cristina</ForeName><Initials>KC</Initials><AffiliationInfo><Affiliation>Post-Graduate Program in Pharmaceutical Sciences, Federal University of Pará, Belém, 66075-110 PA, Brazil. kellyoalbuquerque@hotmail.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Miranda Carvalho</LastName><ForeName>Josiwander</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>College of Chemistry, Federal University of Pará, Belém, 66075-110 PA, Brazil. mcwander@hotmail.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ferreira Reis</LastName><ForeName>Jordano</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Faculty of Pharmacy, Federal University of Pará, Belém, 66075-110 PA, Brazil. jordanoreis@outlook.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vinhal Faria</LastName><ForeName>Lara</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Faculty of Pharmacy, Federal University of Pará, Belém, 66075-110 PA, Brazil. laravf24@gmail.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Coelho-Ferreira</LastName><ForeName>Márlia</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Emílio Goeldi Paraense Museum, Coordination of Botany, Ministry of Science, Technology, Innovation and Communications. Belém, 66077-530 PA, Brazil. mcoelho@museu-goeldi.br.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Silveira</LastName><ForeName>Fernando Tobias</ForeName><Initials>FT</Initials><AffiliationInfo><Affiliation>Evandro Chagas Institute, National Health Foundation, BR-316 Highway km 7, Ananindeua, 67030-000 PA, Brazil. fernandotobias@iec.pa.gov.br.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>da Silva Carneiro</LastName><ForeName>Agnaldo</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Faculty of Pharmacy, Federal University of Pará, Belém, 66075-110 PA, Brazil. agsicar@yahoo.com.br.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Percário</LastName><ForeName>Sandro</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>US Centers for Disease Control and Prevention (CDC), Atlanta, 30329 GA, USA. percario@ufpa.br.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Oxidative Stress Research Lab, Institute of Biological Sciences (ICB), Federal University of Pará, Belém, 66075-110 PA, Brazil. percario@ufpa.br.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>do Rosário Marinho</LastName><ForeName>Andrey Moacir</ForeName><Initials>AM</Initials><AffiliationInfo><Affiliation>College of Chemistry, Federal University of Pará, Belém, 66075-110 PA, Brazil. andrey@ufpa.br.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dolabela</LastName><ForeName>Maria Fâni</ForeName><Initials>MF</Initials><AffiliationInfo><Affiliation>Post-Graduate Program in Pharmaceutical Sciences, Federal University of Pará, Belém, 66075-110 PA, Brazil. fanidolabela20@gmail.com.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Post-Graduate Program in Pharmaceutical Innovation, Federal University of Pará, Belém, 66075-110 PA, Brazil. fanidolabela20@gmail.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>310608/2013-9</GrantID><Agency>Conselho Nacional de Desenvolvimento Científico e Tecnológico</Agency><Country></Country></Grant><Grant><GrantID>Universal; 483894/2012-5</GrantID><Agency>Conselho Nacional de Desenvolvimento Científico e Tecnológico</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>02</Month><Day>21</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Molecules</MedlineTA><NlmUniqueID>100964009</NlmUniqueID><ISSNLinking>1420-3049</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000981">Antiprotozoal Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002243">Carbolines</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D026121">Indole Alkaloids</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010936">Plant Extracts</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015800">Protozoan Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>37691-11-5</RegistryNumber><NameOfSubstance UI="D000976">Antipain</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.4.21.-</RegistryNumber><NameOfSubstance UI="D012697">Serine Endopeptidases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.4.21.83</RegistryNumber><NameOfSubstance UI="C090703">oligopeptidase B</NameOfSubstance></Chemical><Chemical><RegistryNumber>JIG1Q7Z9UH</RegistryNumber><NameOfSubstance UI="C034262">flavopereirine</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000976" MajorTopicYN="N">Antipain</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000981" MajorTopicYN="N">Antiprotozoal Agents</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D029062" MajorTopicYN="N">Apocynaceae</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002243" MajorTopicYN="N">Carbolines</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002470" MajorTopicYN="N">Cell Survival</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D026121" MajorTopicYN="N">Indole Alkaloids</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020128" MajorTopicYN="N">Inhibitory Concentration 50</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007894" MajorTopicYN="N">Leishmania mexicana</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008018" MajorTopicYN="N">Life Cycle Stages</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D062105" MajorTopicYN="N">Molecular Docking Simulation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D056004" MajorTopicYN="N">Molecular Dynamics Simulation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D024301" MajorTopicYN="N">Plant Bark</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010936" MajorTopicYN="N">Plant Extracts</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015800" MajorTopicYN="N">Protozoan Proteins</DescriptorName><QualifierName UI="Q000037" MajorTopicYN="Y">antagonists & inhibitors</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012697" MajorTopicYN="N">Serine Endopeptidases</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000074084" MajorTopicYN="N">THP-1 Cells</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Geissospermum vellosii</Keyword><Keyword MajorTopicYN="N">apocynaceae</Keyword><Keyword MajorTopicYN="N">flavopereirine</Keyword><Keyword MajorTopicYN="N">in silico modeling</Keyword><Keyword MajorTopicYN="N">leishmania</Keyword><Keyword MajorTopicYN="N">medicinal chemistry</Keyword><Keyword MajorTopicYN="N">oligopeptidase B</Keyword></KeywordList><CoiStatement>The authors declare no conflict of interest.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>12</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>01</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>01</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>2</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>2</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>6</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30795632</ArticleId><ArticleId IdType="pii">molecules24040785</ArticleId><ArticleId IdType="doi">10.3390/molecules24040785</ArticleId><ArticleId IdType="pmc">PMC6412932</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Acc Chem Res. 2000 Dec;33(12):889-97</Citation><ArticleIdList><ArticleId IdType="pubmed">11123888</ArticleId></ArticleIdList></Reference><Reference><Citation>Infect Immun. 2001 Apr;69(4):2757-61</Citation><ArticleIdList><ArticleId IdType="pubmed">11254649</ArticleId></ArticleIdList></Reference><Reference><Citation>Adv Drug Deliv Rev. 2001 Mar 1;46(1-3):3-26</Citation><ArticleIdList><ArticleId IdType="pubmed">11259830</ArticleId></ArticleIdList></Reference><Reference><Citation>J Nat Prod. 2002 Jan;65(1):85-8</Citation><ArticleIdList><ArticleId IdType="pubmed">11809075</ArticleId></ArticleIdList></Reference><Reference><Citation>J Comput Chem. 2004 Jul 15;25(9):1157-74</Citation><ArticleIdList><ArticleId IdType="pubmed">15116359</ArticleId></ArticleIdList></Reference><Reference><Citation>Mem Inst Oswaldo Cruz. 2004 May;99(3):325-7</Citation><ArticleIdList><ArticleId IdType="pubmed">15273808</ArticleId></ArticleIdList></Reference><Reference><Citation>NeuroRx. 2005 Oct;2(4):541-53</Citation><ArticleIdList><ArticleId IdType="pubmed">16489364</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2006 Jul 15;22(14):1710-6</Citation><ArticleIdList><ArticleId IdType="pubmed">16632493</ArticleId></ArticleIdList></Reference><Reference><Citation>J Med Chem. 2006 Jun 1;49(11):3315-21</Citation><ArticleIdList><ArticleId IdType="pubmed">16722650</ArticleId></ArticleIdList></Reference><Reference><Citation>Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Aug 1;62(Pt 8):808-10</Citation><ArticleIdList><ArticleId IdType="pubmed">16880564</ArticleId></ArticleIdList></Reference><Reference><Citation>Phytomedicine. 2007 Jun;14(6):377-80</Citation><ArticleIdList><ArticleId IdType="pubmed">17140782</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochimie. 2008 Feb;90(2):336-44</Citation><ArticleIdList><ArticleId IdType="pubmed">18029266</ArticleId></ArticleIdList></Reference><Reference><Citation>J Phys Chem B. 2008 Jul 31;112(30):9020-41</Citation><ArticleIdList><ArticleId IdType="pubmed">18593145</ArticleId></ArticleIdList></Reference><Reference><Citation>J Ethnopharmacol. 2009 Aug 17;125(1):163-9</Citation><ArticleIdList><ArticleId IdType="pubmed">19497357</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Biochem Parasitol. 2011 Jan;175(1):49-57</Citation><ArticleIdList><ArticleId IdType="pubmed">20883728</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2010 Dec 10;285(50):39249-59</Citation><ArticleIdList><ArticleId IdType="pubmed">20926390</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2011 Jan 7;286(1):429-40</Citation><ArticleIdList><ArticleId IdType="pubmed">20961853</ArticleId></ArticleIdList></Reference><Reference><Citation>J Ethnopharmacol. 2012 Jan 31;139(2):471-7</Citation><ArticleIdList><ArticleId IdType="pubmed">22143154</ArticleId></ArticleIdList></Reference><Reference><Citation>J Nat Prod. 2012 May 25;75(5):928-34</Citation><ArticleIdList><ArticleId IdType="pubmed">22551062</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Prod Commun. 2013 Nov;8(11):1541-4</Citation><ArticleIdList><ArticleId IdType="pubmed">24427936</ArticleId></ArticleIdList></Reference><Reference><Citation>Drug Discov Today Technol. 2004 Dec;1(4):337-41</Citation><ArticleIdList><ArticleId IdType="pubmed">24981612</ArticleId></ArticleIdList></Reference><Reference><Citation>Expert Opin Drug Discov. 2015 May;10(5):449-61</Citation><ArticleIdList><ArticleId IdType="pubmed">25835573</ArticleId></ArticleIdList></Reference><Reference><Citation>Fitoterapia. 2015 Jun;103:192-6</Citation><ArticleIdList><ArticleId IdType="pubmed">25863351</ArticleId></ArticleIdList></Reference><Reference><Citation>Malar J. 2015 Mar 27;14:132</Citation><ArticleIdList><ArticleId IdType="pubmed">25888719</ArticleId></ArticleIdList></Reference><Reference><Citation>J Comput Chem. 2015 Sep 30;36(25):1859-73</Citation><ArticleIdList><ArticleId IdType="pubmed">26216222</ArticleId></ArticleIdList></Reference><Reference><Citation>J Chem Theory Comput. 2015 Oct 13;11(10):4770-9</Citation><ArticleIdList><ArticleId IdType="pubmed">26574266</ArticleId></ArticleIdList></Reference><Reference><Citation>J Chem Theory Comput. 2013 Jul 9;9(7):3084-95</Citation><ArticleIdList><ArticleId IdType="pubmed">26583988</ArticleId></ArticleIdList></Reference><Reference><Citation>J Chem Theory Comput. 2012 Sep 11;8(9):3314-21</Citation><ArticleIdList><ArticleId IdType="pubmed">26605738</ArticleId></ArticleIdList></Reference><Reference><Citation>Pharmacogn Mag. 2016 Jan;12(Suppl 1):S96-S101</Citation><ArticleIdList><ArticleId IdType="pubmed">27041867</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Inform. 2012 Feb;31(2):114-22</Citation><ArticleIdList><ArticleId IdType="pubmed">27476956</ArticleId></ArticleIdList></Reference><Reference><Citation>Braz J Med Biol Res. 2016 Oct 24;49(11):e5644</Citation><ArticleIdList><ArticleId IdType="pubmed">27783811</ArticleId></ArticleIdList></Reference><Reference><Citation>Molecules. 2017 Jul 14;22(7):null</Citation><ArticleIdList><ArticleId IdType="pubmed">28708094</ArticleId></ArticleIdList></Reference><Reference><Citation>Arch Pharm Res. 2018 Feb;41(2):208-218</Citation><ArticleIdList><ArticleId IdType="pubmed">29243039</ArticleId></ArticleIdList></Reference><Reference><Citation>J Immunol Methods. 1983 Dec 16;65(1-2):55-63</Citation><ArticleIdList><ArticleId IdType="pubmed">6606682</ArticleId></ArticleIdList></Reference><Reference><Citation>Arch Biochem Biophys. 1995 Apr 20;318(2):247-50</Citation><ArticleIdList><ArticleId IdType="pubmed">7733651</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1999 Jan 1;27(1):325-31</Citation><ArticleIdList><ArticleId IdType="pubmed">9847218</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/BiopestPeptidV1/Data/Main/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000468 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd -nk 000468 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= BiopestPeptidV1
|flux= Main
|étape= Corpus
|type= RBID
|clé= pubmed:30795632
|texte= Flavopereirine-An Alkaloid Derived from Geissospermum vellosii-Presents Leishmanicidal Activity In Vitro.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Corpus/RBID.i -Sk "pubmed:30795632" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a BiopestPeptidV1
| This area was generated with Dilib version V0.6.38. |  |