Stable genetic transformation of intact Nicotiana cells by the particle bombardment process.
Identifieur interne : 000966 ( Main/Exploration ); précédent : 000965; suivant : 000967Stable genetic transformation of intact Nicotiana cells by the particle bombardment process.
Auteurs : T M Klein [États-Unis] ; E C Harper ; Z. Svab ; J C Sanford ; M E Fromm ; P. MaligaSource :
- Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 1988.
Abstract
We show that the genetic transformation of Nicotiana tabacum can be achieved by bombarding intact cells and tissues with DNA-coated particles. Leaves or suspension culture cells were treated with tungsten microprojectiles carrying plasmid DNA containing a neomycin phosphotransferase gene. Callus harboring the foreign gene was recovered from the bombarded tissue by selection on medium containing kanamycin. Kanamycin-resistant plants have subsequently been regenerated from the callus derived from leaves. Transient expression of an introduced beta-glucuronidase gene was used to assess the efficiency of DNA delivery by microprojectiles. The frequency of cells that were stably transformed with the neomycin phosphotransferase gene was a few percent of the cells that transiently expressed the beta-glucuronidase gene. These results show that gene transfer by high-velocity microprojectiles is a rapid and direct means for transforming intact plant cells and tissues that eliminates the need for production of protoplasts or infection by Agrobacterium.
DOI: 10.1073/pnas.85.22.8502
PubMed: 16593993
PubMed Central: PMC282486
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Stable genetic transformation of intact Nicotiana cells by the particle bombardment process.</title>
<author><name sortKey="Klein, T M" sort="Klein, T M" uniqKey="Klein T" first="T M" last="Klein">T M Klein</name>
<affiliation wicri:level="2"><nlm:affiliation>Plant Gene Expression Center, U.S. Department of Agriculture, Agricultural Research Service, 800 Buchanan Street, Albany, CA 94710.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Californie</region>
</placeName>
<wicri:cityArea>Plant Gene Expression Center, U.S. Department of Agriculture, Agricultural Research Service, 800 Buchanan Street, Albany</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Harper, E C" sort="Harper, E C" uniqKey="Harper E" first="E C" last="Harper">E C Harper</name>
</author>
<author><name sortKey="Svab, Z" sort="Svab, Z" uniqKey="Svab Z" first="Z" last="Svab">Z. Svab</name>
</author>
<author><name sortKey="Sanford, J C" sort="Sanford, J C" uniqKey="Sanford J" first="J C" last="Sanford">J C Sanford</name>
</author>
<author><name sortKey="Fromm, M E" sort="Fromm, M E" uniqKey="Fromm M" first="M E" last="Fromm">M E Fromm</name>
</author>
<author><name sortKey="Maliga, P" sort="Maliga, P" uniqKey="Maliga P" first="P" last="Maliga">P. Maliga</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="1988">1988</date>
<idno type="RBID">pubmed:16593993</idno>
<idno type="pmid">16593993</idno>
<idno type="pmc">PMC282486</idno>
<idno type="doi">10.1073/pnas.85.22.8502</idno>
<idno type="wicri:Area/Main/Corpus">000965</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000965</idno>
<idno type="wicri:Area/Main/Curation">000965</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000965</idno>
<idno type="wicri:Area/Main/Exploration">000965</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Stable genetic transformation of intact Nicotiana cells by the particle bombardment process.</title>
<author><name sortKey="Klein, T M" sort="Klein, T M" uniqKey="Klein T" first="T M" last="Klein">T M Klein</name>
<affiliation wicri:level="2"><nlm:affiliation>Plant Gene Expression Center, U.S. Department of Agriculture, Agricultural Research Service, 800 Buchanan Street, Albany, CA 94710.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Californie</region>
</placeName>
<wicri:cityArea>Plant Gene Expression Center, U.S. Department of Agriculture, Agricultural Research Service, 800 Buchanan Street, Albany</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Harper, E C" sort="Harper, E C" uniqKey="Harper E" first="E C" last="Harper">E C Harper</name>
</author>
<author><name sortKey="Svab, Z" sort="Svab, Z" uniqKey="Svab Z" first="Z" last="Svab">Z. Svab</name>
</author>
<author><name sortKey="Sanford, J C" sort="Sanford, J C" uniqKey="Sanford J" first="J C" last="Sanford">J C Sanford</name>
</author>
<author><name sortKey="Fromm, M E" sort="Fromm, M E" uniqKey="Fromm M" first="M E" last="Fromm">M E Fromm</name>
</author>
<author><name sortKey="Maliga, P" sort="Maliga, P" uniqKey="Maliga P" first="P" last="Maliga">P. Maliga</name>
</author>
</analytic>
<series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title>
<idno type="ISSN">0027-8424</idno>
<imprint><date when="1988" type="published">1988</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">We show that the genetic transformation of Nicotiana tabacum can be achieved by bombarding intact cells and tissues with DNA-coated particles. Leaves or suspension culture cells were treated with tungsten microprojectiles carrying plasmid DNA containing a neomycin phosphotransferase gene. Callus harboring the foreign gene was recovered from the bombarded tissue by selection on medium containing kanamycin. Kanamycin-resistant plants have subsequently been regenerated from the callus derived from leaves. Transient expression of an introduced beta-glucuronidase gene was used to assess the efficiency of DNA delivery by microprojectiles. The frequency of cells that were stably transformed with the neomycin phosphotransferase gene was a few percent of the cells that transiently expressed the beta-glucuronidase gene. These results show that gene transfer by high-velocity microprojectiles is a rapid and direct means for transforming intact plant cells and tissues that eliminates the need for production of protoplasts or infection by Agrobacterium.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">16593993</PMID>
<DateCompleted><Year>2010</Year>
<Month>06</Month>
<Day>30</Day>
</DateCompleted>
<DateRevised><Year>2019</Year>
<Month>05</Month>
<Day>01</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0027-8424</ISSN>
<JournalIssue CitedMedium="Print"><Volume>85</Volume>
<Issue>22</Issue>
<PubDate><Year>1988</Year>
<Month>Nov</Month>
</PubDate>
</JournalIssue>
<Title>Proceedings of the National Academy of Sciences of the United States of America</Title>
<ISOAbbreviation>Proc Natl Acad Sci U S A</ISOAbbreviation>
</Journal>
<ArticleTitle>Stable genetic transformation of intact Nicotiana cells by the particle bombardment process.</ArticleTitle>
<Pagination><MedlinePgn>8502-5</MedlinePgn>
</Pagination>
<Abstract><AbstractText>We show that the genetic transformation of Nicotiana tabacum can be achieved by bombarding intact cells and tissues with DNA-coated particles. Leaves or suspension culture cells were treated with tungsten microprojectiles carrying plasmid DNA containing a neomycin phosphotransferase gene. Callus harboring the foreign gene was recovered from the bombarded tissue by selection on medium containing kanamycin. Kanamycin-resistant plants have subsequently been regenerated from the callus derived from leaves. Transient expression of an introduced beta-glucuronidase gene was used to assess the efficiency of DNA delivery by microprojectiles. The frequency of cells that were stably transformed with the neomycin phosphotransferase gene was a few percent of the cells that transiently expressed the beta-glucuronidase gene. These results show that gene transfer by high-velocity microprojectiles is a rapid and direct means for transforming intact plant cells and tissues that eliminates the need for production of protoplasts or infection by Agrobacterium.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Klein</LastName>
<ForeName>T M</ForeName>
<Initials>TM</Initials>
<AffiliationInfo><Affiliation>Plant Gene Expression Center, U.S. Department of Agriculture, Agricultural Research Service, 800 Buchanan Street, Albany, CA 94710.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Harper</LastName>
<ForeName>E C</ForeName>
<Initials>EC</Initials>
</Author>
<Author ValidYN="Y"><LastName>Svab</LastName>
<ForeName>Z</ForeName>
<Initials>Z</Initials>
</Author>
<Author ValidYN="Y"><LastName>Sanford</LastName>
<ForeName>J C</ForeName>
<Initials>JC</Initials>
</Author>
<Author ValidYN="Y"><LastName>Fromm</LastName>
<ForeName>M E</ForeName>
<Initials>ME</Initials>
</Author>
<Author ValidYN="Y"><LastName>Maliga</LastName>
<ForeName>P</ForeName>
<Initials>P</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>Proc Natl Acad Sci U S A</MedlineTA>
<NlmUniqueID>7505876</NlmUniqueID>
<ISSNLinking>0027-8424</ISSNLinking>
</MedlineJournalInfo>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1988</Year>
<Month>11</Month>
<Day>1</Day>
<Hour>0</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>1988</Year>
<Month>11</Month>
<Day>1</Day>
<Hour>0</Hour>
<Minute>1</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>1988</Year>
<Month>11</Month>
<Day>1</Day>
<Hour>0</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">16593993</ArticleId>
<ArticleId IdType="pmc">PMC282486</ArticleId>
<ArticleId IdType="doi">10.1073/pnas.85.22.8502</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>J Pediatr. 1988 Sep;113(3):559-63</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3411404</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 1988 Apr 8;240(4849):204-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2832947</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 1987 Jun 5;236(4806):1259-62</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17770329</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 1986 Feb 27-Mar 5;319(6056):791-3</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3005872</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 1986 Aug;83(15):5602-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3016708</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Gen Genet. 1988 Apr;212(1):182-90</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3163765</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Methods Enzymol. 1987;153:3-11</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3323803</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>EMBO J. 1987 Dec 20;6(13):3901-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3327686</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 1987 Apr 3;236(4797):48-54</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17759205</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Exp Cell Res. 1968 Apr;50(1):151-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">5650857</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 1987 Sep 4;237(4819):1176-83</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17801640</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Mol Appl Genet. 1982;1(6):499-511</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7153687</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell. 1980 Mar;19(3):729-39</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7363328</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>EMBO J. 1984 Aug;3(8):1681-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16453538</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>EMBO J. 1984 Dec 1;3(12):2717-22</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16453573</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 1988 Jun;85(12):4305-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16593942</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>DNA. 1986 Apr;5(2):101-13</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3519133</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>Californie</li>
</region>
</list>
<tree><noCountry><name sortKey="Fromm, M E" sort="Fromm, M E" uniqKey="Fromm M" first="M E" last="Fromm">M E Fromm</name>
<name sortKey="Harper, E C" sort="Harper, E C" uniqKey="Harper E" first="E C" last="Harper">E C Harper</name>
<name sortKey="Maliga, P" sort="Maliga, P" uniqKey="Maliga P" first="P" last="Maliga">P. Maliga</name>
<name sortKey="Sanford, J C" sort="Sanford, J C" uniqKey="Sanford J" first="J C" last="Sanford">J C Sanford</name>
<name sortKey="Svab, Z" sort="Svab, Z" uniqKey="Svab Z" first="Z" last="Svab">Z. Svab</name>
</noCountry>
<country name="États-Unis"><region name="Californie"><name sortKey="Klein, T M" sort="Klein, T M" uniqKey="Klein T" first="T M" last="Klein">T M Klein</name>
</region>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/AgrobacTransV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000966 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000966 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Bois |area= AgrobacTransV1 |flux= Main |étape= Exploration |type= RBID |clé= pubmed:16593993 |texte= Stable genetic transformation of intact Nicotiana cells by the particle bombardment process. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:16593993" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \ | NlmPubMed2Wicri -a AgrobacTransV1
This area was generated with Dilib version V0.6.38. |