AgrobacTransV1, Main, Exploration, bibRecord, 000855

[1.4 kb 5' flanking region of class I patatin directs tuber-specific gus expression in potato (Solanum tuberosum L.)].

Identifieur interne : 000855 ( Main/Exploration ); précédent : 000854; suivant : 000856

[1.4 kb 5' flanking region of class I patatin directs tuber-specific gus expression in potato (Solanum tuberosum L.)].

Auteurs : D G Song ; J. Zhou ; D Q Huang ; H. Ma ; J F Situ ; G Q Wang ; X M Wang

Source :

Shi yan sheng wu xue bao [ 0001-5334 ] ; 2001.

RBID : pubmed:12549004

Descripteurs français

English descriptors

KwdEn :
- Carboxylic Ester Hydrolases (genetics), Carboxylic Ester Hydrolases (metabolism), Gene Expression Regulation (MeSH), Glucuronidase (genetics), Glucuronidase (metabolism), Plant Proteins (genetics), Plant Proteins (metabolism), Plants, Genetically Modified (MeSH), Solanum tuberosum (genetics).
MESH :
- chemical , genetics : Carboxylic Ester Hydrolases, Glucuronidase, Plant Proteins.
- chemical , metabolism : Carboxylic Ester Hydrolases, Glucuronidase, Plant Proteins.
- genetics : Solanum tuberosum.
- Gene Expression Regulation, Plants, Genetically Modified.

Abstract

Binary vectors pPATIs (with partial signal sequence) and pPATI (without signal sequence) were constructed by fusing 1.4 kb 5' flanking regions of Class I patatin gene with GUS. Transient GUS expression was observed in in vitro tuber slices bombarded with pPATI. These constructs were then introduced into potato (cv. Desiree) via Agrobacterium tumefaciens transformation. Transgenic potato plants were confirmed by X-Gluc staining and PCR. Using in vitro tuberization system, GUS activities were assayed by fluorescence. It was shown that, in plants transformed with PATI-GUS, GUS specific activities were about 10-20 fold higher in tubers than in stems. Increased sucrose concentration could not induce PATI-GUS expression, but light enhanced PATI-GUS expression in cultured shoots.

PubMed: 12549004

Affiliations:

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Le document en format XML

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<author><name sortKey="Song, D G" sort="Song, D G" uniqKey="Song D" first="D G" last="Song">D G Song</name>
<affiliation><nlm:affiliation>State Key Laboratory of Genetics and Genetic Engineering, Institute of Genetics, Fudan University, Shanghai 200433.</nlm:affiliation>
<wicri:noCountry code="subField">Shanghai 200433</wicri:noCountry>
</affiliation>
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<author><name sortKey="Zhou, J" sort="Zhou, J" uniqKey="Zhou J" first="J" last="Zhou">J. Zhou</name>
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<author><name sortKey="Huang, D Q" sort="Huang, D Q" uniqKey="Huang D" first="D Q" last="Huang">D Q Huang</name>
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<author><name sortKey="Ma, H" sort="Ma, H" uniqKey="Ma H" first="H" last="Ma">H. Ma</name>
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<author><name sortKey="Situ, J F" sort="Situ, J F" uniqKey="Situ J" first="J F" last="Situ">J F Situ</name>
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<author><name sortKey="Wang, G Q" sort="Wang, G Q" uniqKey="Wang G" first="G Q" last="Wang">G Q Wang</name>
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<author><name sortKey="Wang, X M" sort="Wang, X M" uniqKey="Wang X" first="X M" last="Wang">X M Wang</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">[1.4 kb 5' flanking region of class I patatin directs tuber-specific gus expression in potato (Solanum tuberosum L.)].</title>
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<author><name sortKey="Wang, G Q" sort="Wang, G Q" uniqKey="Wang G" first="G Q" last="Wang">G Q Wang</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Carboxylic Ester Hydrolases (genetics)</term>
<term>Carboxylic Ester Hydrolases (metabolism)</term>
<term>Gene Expression Regulation (MeSH)</term>
<term>Glucuronidase (genetics)</term>
<term>Glucuronidase (metabolism)</term>
<term>Plant Proteins (genetics)</term>
<term>Plant Proteins (metabolism)</term>
<term>Plants, Genetically Modified (MeSH)</term>
<term>Solanum tuberosum (genetics)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Carboxylic ester hydrolases (génétique)</term>
<term>Carboxylic ester hydrolases (métabolisme)</term>
<term>Glucuronidase (génétique)</term>
<term>Glucuronidase (métabolisme)</term>
<term>Protéines végétales (génétique)</term>
<term>Protéines végétales (métabolisme)</term>
<term>Régulation de l'expression des gènes (MeSH)</term>
<term>Solanum tuberosum (génétique)</term>
<term>Végétaux génétiquement modifiés (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Carboxylic Ester Hydrolases</term>
<term>Glucuronidase</term>
<term>Plant Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Carboxylic Ester Hydrolases</term>
<term>Glucuronidase</term>
<term>Plant Proteins</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Solanum tuberosum</term>
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<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Carboxylic ester hydrolases</term>
<term>Glucuronidase</term>
<term>Protéines végétales</term>
<term>Solanum tuberosum</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Carboxylic ester hydrolases</term>
<term>Glucuronidase</term>
<term>Protéines végétales</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation</term>
<term>Plants, Genetically Modified</term>
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<front><div type="abstract" xml:lang="en">Binary vectors pPATIs (with partial signal sequence) and pPATI (without signal sequence) were constructed by fusing 1.4 kb 5' flanking regions of Class I patatin gene with GUS. Transient GUS expression was observed in in vitro tuber slices bombarded with pPATI. These constructs were then introduced into potato (cv. Desiree) via Agrobacterium tumefaciens transformation. Transgenic potato plants were confirmed by X-Gluc staining and PCR. Using in vitro tuberization system, GUS activities were assayed by fluorescence. It was shown that, in plants transformed with PATI-GUS, GUS specific activities were about 10-20 fold higher in tubers than in stems. Increased sucrose concentration could not induce PATI-GUS expression, but light enhanced PATI-GUS expression in cultured shoots.</div>
</front>
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<DateCompleted><Year>2003</Year>
<Month>05</Month>
<Day>06</Day>
</DateCompleted>
<DateRevised><Year>2006</Year>
<Month>11</Month>
<Day>15</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0001-5334</ISSN>
<JournalIssue CitedMedium="Print"><Volume>34</Volume>
<Issue>1</Issue>
<PubDate><Year>2001</Year>
<Month>Mar</Month>
</PubDate>
</JournalIssue>
<Title>Shi yan sheng wu xue bao</Title>
<ISOAbbreviation>Shi Yan Sheng Wu Xue Bao</ISOAbbreviation>
</Journal>
<ArticleTitle>[1.4 kb 5' flanking region of class I patatin directs tuber-specific gus expression in potato (Solanum tuberosum L.)].</ArticleTitle>
<Pagination><MedlinePgn>5-10</MedlinePgn>
</Pagination>
<Abstract><AbstractText>Binary vectors pPATIs (with partial signal sequence) and pPATI (without signal sequence) were constructed by fusing 1.4 kb 5' flanking regions of Class I patatin gene with GUS. Transient GUS expression was observed in in vitro tuber slices bombarded with pPATI. These constructs were then introduced into potato (cv. Desiree) via Agrobacterium tumefaciens transformation. Transgenic potato plants were confirmed by X-Gluc staining and PCR. Using in vitro tuberization system, GUS activities were assayed by fluorescence. It was shown that, in plants transformed with PATI-GUS, GUS specific activities were about 10-20 fold higher in tubers than in stems. Increased sucrose concentration could not induce PATI-GUS expression, but light enhanced PATI-GUS expression in cultured shoots.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Song</LastName>
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<AffiliationInfo><Affiliation>State Key Laboratory of Genetics and Genetic Engineering, Institute of Genetics, Fudan University, Shanghai 200433.</Affiliation>
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<Author ValidYN="Y"><LastName>Zhou</LastName>
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<Author ValidYN="Y"><LastName>Huang</LastName>
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<MedlineJournalInfo><Country>China</Country>
<MedlineTA>Shi Yan Sheng Wu Xue Bao</MedlineTA>
<NlmUniqueID>0413570</NlmUniqueID>
<ISSNLinking>0001-5334</ISSNLinking>
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<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance>
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<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C043222">patatin protein, Solanum tuberosum</NameOfSubstance>
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<Chemical><RegistryNumber>EC 3.1.1.-</RegistryNumber>
<NameOfSubstance UI="D002265">Carboxylic Ester Hydrolases</NameOfSubstance>
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<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
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<MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName>
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<MeshHeading><DescriptorName UI="D011198" MajorTopicYN="N">Solanum tuberosum</DescriptorName>
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<name sortKey="Wang, X M" sort="Wang, X M" uniqKey="Wang X" first="X M" last="Wang">X M Wang</name>
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	Serveur d'exploration sur l'agrobacterium et la transgénèse
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Serveur d'exploration sur l'agrobacterium et la transgénèse

[1.4 kb 5' flanking region of class I patatin directs tuber-specific gus expression in potato (Solanum tuberosum L.)].

[1.4 kb 5' flanking region of class I patatin directs tuber-specific gus expression in potato (Solanum tuberosum L.)].

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki