Agroinjection of tomato fruits. A tool for rapid functional analysis of transgenes directly in fruit.
Identifieur interne : 000766 ( Main/Exploration ); précédent : 000765; suivant : 000767Agroinjection of tomato fruits. A tool for rapid functional analysis of transgenes directly in fruit.
Auteurs : Diego Orzaez [Espagne] ; Sophie Mirabel ; Willemien H. Wieland ; Antonio GranellSource :
- Plant physiology [ 0032-0889 ] ; 2006.
Descripteurs français
- KwdFr :
- Anticorps hétérophiles (biosynthèse), Biotechnologie (méthodes), Caulimovirus (génétique), Extinction de l'expression des gènes (MeSH), Fruit (génétique), Fruit (microbiologie), Glucuronidase (génétique), Glucuronidase (métabolisme), Gènes rapporteurs (MeSH), Injections (MeSH), Lycopersicon esculentum (anatomie et histologie), Lycopersicon esculentum (génétique), Lycopersicon esculentum (microbiologie), Microscopie confocale (MeSH), Oxidoreductases (génétique), Oxidoreductases (métabolisme), Plasmides (génétique), Protéines bactériennes (génétique), Protéines bactériennes (métabolisme), Protéines luminescentes (génétique), Protéines luminescentes (métabolisme), Rhizobium (croissance et développement), Rhizobium (génétique), Transformation génétique (MeSH), Transgènes (physiologie), Végétaux génétiquement modifiés (génétique), Végétaux génétiquement modifiés (microbiologie).
- MESH :
- anatomie et histologie : Lycopersicon esculentum.
- biosynthèse : Anticorps hétérophiles.
- croissance et développement : Rhizobium.
- génétique : Caulimovirus, Fruit, Glucuronidase, Lycopersicon esculentum, Oxidoreductases, Plasmides, Protéines bactériennes, Protéines luminescentes, Rhizobium, Végétaux génétiquement modifiés.
- microbiologie : Fruit, Lycopersicon esculentum, Végétaux génétiquement modifiés.
- métabolisme : Glucuronidase, Oxidoreductases, Protéines bactériennes, Protéines luminescentes.
- méthodes : Biotechnologie.
- physiologie : Transgènes.
- Extinction de l'expression des gènes, Gènes rapporteurs, Injections, Microscopie confocale, Transformation génétique.
English descriptors
- KwdEn :
- Antibodies, Heterophile (biosynthesis), Bacterial Proteins (genetics), Bacterial Proteins (metabolism), Biotechnology (methods), Caulimovirus (genetics), Fruit (genetics), Fruit (microbiology), Gene Silencing (MeSH), Genes, Reporter (MeSH), Glucuronidase (genetics), Glucuronidase (metabolism), Injections (MeSH), Luminescent Proteins (genetics), Luminescent Proteins (metabolism), Lycopersicon esculentum (anatomy & histology), Lycopersicon esculentum (genetics), Lycopersicon esculentum (microbiology), Microscopy, Confocal (MeSH), Oxidoreductases (genetics), Oxidoreductases (metabolism), Plants, Genetically Modified (genetics), Plants, Genetically Modified (microbiology), Plasmids (genetics), Rhizobium (genetics), Rhizobium (growth & development), Transformation, Genetic (MeSH), Transgenes (physiology).
- MESH :
- chemical , biosynthesis : Antibodies, Heterophile.
- chemical , genetics : Bacterial Proteins, Glucuronidase, Luminescent Proteins, Oxidoreductases.
- chemical , metabolism : Bacterial Proteins, Glucuronidase, Luminescent Proteins, Oxidoreductases.
- anatomy & histology : Lycopersicon esculentum.
- genetics : Caulimovirus, Fruit, Lycopersicon esculentum, Plants, Genetically Modified, Plasmids, Rhizobium.
- growth & development : Rhizobium.
- methods : Biotechnology.
- microbiology : Fruit, Lycopersicon esculentum, Plants, Genetically Modified.
- physiology : Transgenes.
- Gene Silencing, Genes, Reporter, Injections, Microscopy, Confocal, Transformation, Genetic.
Abstract
Transient expression of foreign genes in plant tissues is a valuable tool for plant biotechnology. To shorten the time for gene functional analysis in fruits, we developed a transient methodology that could be applied to tomato (Solanum lycopersicum cv Micro Tom) fruits. It was found that injection of Agrobacterium cultures through the fruit stylar apex resulted in complete fruit infiltration. This infiltration method, named fruit agroinjection, rendered high levels of 35S Cauliflower mosaic virus-driven beta-glucuronidase and yellow fluorescence protein transient expression in the fruit, with higher expression levels around the placenta and moderate levels in the pericarp. Usefulness of fruit agroinjection was assayed in three case studies: (1) the heat shock regulation of an Arabidopsis (Arabidopsis thaliana) promoter, (2) the production of recombinant IgA antibodies as an example of molecular farming, and (3) the virus-induced gene silencing of the carotene biosynthesis pathway. In all three instances, this technology was shown to be efficient as a tool for fast transgene expression in fruits.
DOI: 10.1104/pp.105.068221
PubMed: 16403736
PubMed Central: PMC1326026
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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A tool for rapid functional analysis of transgenes directly in fruit.</title><author><name sortKey="Orzaez, Diego" sort="Orzaez, Diego" uniqKey="Orzaez D" first="Diego" last="Orzaez">Diego Orzaez</name><affiliation wicri:level="2"><nlm:affiliation>Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas, Universidad Politécnica de Valencia, Camino de Vera s/n, 46022 Valencia, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas, Universidad Politécnica de Valencia, Camino de Vera s/n, 46022 Valencia</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté valencienne</region></placeName></affiliation></author><author><name sortKey="Mirabel, Sophie" sort="Mirabel, Sophie" uniqKey="Mirabel S" first="Sophie" last="Mirabel">Sophie Mirabel</name></author><author><name sortKey="Wieland, Willemien H" sort="Wieland, Willemien H" uniqKey="Wieland W" first="Willemien H" last="Wieland">Willemien H. 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esculentum (anatomy & histology)</term><term>Lycopersicon esculentum (genetics)</term><term>Lycopersicon esculentum (microbiology)</term><term>Microscopy, Confocal (MeSH)</term><term>Oxidoreductases (genetics)</term><term>Oxidoreductases (metabolism)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (microbiology)</term><term>Plasmids (genetics)</term><term>Rhizobium (genetics)</term><term>Rhizobium (growth & development)</term><term>Transformation, Genetic (MeSH)</term><term>Transgenes (physiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Anticorps hétérophiles (biosynthèse)</term><term>Biotechnologie (méthodes)</term><term>Caulimovirus (génétique)</term><term>Extinction de l'expression des gènes (MeSH)</term><term>Fruit (génétique)</term><term>Fruit (microbiologie)</term><term>Glucuronidase (génétique)</term><term>Glucuronidase (métabolisme)</term><term>Gènes rapporteurs (MeSH)</term><term>Injections (MeSH)</term><term>Lycopersicon esculentum (anatomie et histologie)</term><term>Lycopersicon esculentum (génétique)</term><term>Lycopersicon esculentum (microbiologie)</term><term>Microscopie confocale (MeSH)</term><term>Oxidoreductases (génétique)</term><term>Oxidoreductases (métabolisme)</term><term>Plasmides (génétique)</term><term>Protéines bactériennes (génétique)</term><term>Protéines bactériennes (métabolisme)</term><term>Protéines luminescentes (génétique)</term><term>Protéines luminescentes (métabolisme)</term><term>Rhizobium (croissance et développement)</term><term>Rhizobium (génétique)</term><term>Transformation génétique (MeSH)</term><term>Transgènes (physiologie)</term><term>Végétaux génétiquement modifiés (génétique)</term><term>Végétaux génétiquement modifiés (microbiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Antibodies, Heterophile</term></keywords><keywords scheme="MESH" type="chemical" 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qualifier="métabolisme" xml:lang="fr"><term>Glucuronidase</term><term>Oxidoreductases</term><term>Protéines bactériennes</term><term>Protéines luminescentes</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Biotechnologie</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Transgènes</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Transgenes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Silencing</term><term>Genes, Reporter</term><term>Injections</term><term>Microscopy, Confocal</term><term>Transformation, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Extinction de l'expression des gènes</term><term>Gènes rapporteurs</term><term>Injections</term><term>Microscopie confocale</term><term>Transformation génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Transient expression of foreign genes in plant tissues is a valuable tool for plant biotechnology. To shorten the time for gene functional analysis in fruits, we developed a transient methodology that could be applied to tomato (Solanum lycopersicum cv Micro Tom) fruits. It was found that injection of Agrobacterium cultures through the fruit stylar apex resulted in complete fruit infiltration. This infiltration method, named fruit agroinjection, rendered high levels of 35S Cauliflower mosaic virus-driven beta-glucuronidase and yellow fluorescence protein transient expression in the fruit, with higher expression levels around the placenta and moderate levels in the pericarp. Usefulness of fruit agroinjection was assayed in three case studies: (1) the heat shock regulation of an Arabidopsis (Arabidopsis thaliana) promoter, (2) the production of recombinant IgA antibodies as an example of molecular farming, and (3) the virus-induced gene silencing of the carotene biosynthesis pathway. In all three instances, this technology was shown to be efficient as a tool for fast transgene expression in fruits.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16403736</PMID><DateCompleted><Year>2006</Year><Month>04</Month><Day>20</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0032-0889</ISSN><JournalIssue CitedMedium="Print"><Volume>140</Volume><Issue>1</Issue><PubDate><Year>2006</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Plant physiology</Title><ISOAbbreviation>Plant Physiol</ISOAbbreviation></Journal><ArticleTitle>Agroinjection of tomato fruits. A tool for rapid functional analysis of transgenes directly in fruit.</ArticleTitle><Pagination><MedlinePgn>3-11</MedlinePgn></Pagination><Abstract><AbstractText>Transient expression of foreign genes in plant tissues is a valuable tool for plant biotechnology. To shorten the time for gene functional analysis in fruits, we developed a transient methodology that could be applied to tomato (Solanum lycopersicum cv Micro Tom) fruits. It was found that injection of Agrobacterium cultures through the fruit stylar apex resulted in complete fruit infiltration. This infiltration method, named fruit agroinjection, rendered high levels of 35S Cauliflower mosaic virus-driven beta-glucuronidase and yellow fluorescence protein transient expression in the fruit, with higher expression levels around the placenta and moderate levels in the pericarp. Usefulness of fruit agroinjection was assayed in three case studies: (1) the heat shock regulation of an Arabidopsis (Arabidopsis thaliana) promoter, (2) the production of recombinant IgA antibodies as an example of molecular farming, and (3) the virus-induced gene silencing of the carotene biosynthesis pathway. In all three instances, this technology was shown to be efficient as a tool for fast transgene expression in fruits.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Orzaez</LastName><ForeName>Diego</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas, Universidad Politécnica de Valencia, Camino de Vera s/n, 46022 Valencia, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mirabel</LastName><ForeName>Sophie</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Wieland</LastName><ForeName>Willemien H</ForeName><Initials>WH</Initials></Author><Author ValidYN="Y"><LastName>Granell</LastName><ForeName>Antonio</ForeName><Initials>A</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D023362">Evaluation Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plant Physiol</MedlineTA><NlmUniqueID>0401224</NlmUniqueID><ISSNLinking>0032-0889</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000910">Antibodies, Heterophile</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001426">Bacterial Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008164">Luminescent Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C054562">yellow fluorescent protein, Bacteria</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.-</RegistryNumber><NameOfSubstance UI="D010088">Oxidoreductases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.14.99.-</RegistryNumber><NameOfSubstance UI="C022398">phytoene dehydrogenase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.31</RegistryNumber><NameOfSubstance UI="D005966">Glucuronidase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000910" MajorTopicYN="N">Antibodies, Heterophile</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001426" MajorTopicYN="N">Bacterial Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001709" MajorTopicYN="N">Biotechnology</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017796" MajorTopicYN="N">Caulimovirus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005638" MajorTopicYN="N">Fruit</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020868" MajorTopicYN="N">Gene Silencing</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017930" MajorTopicYN="N">Genes, Reporter</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005966" MajorTopicYN="N">Glucuronidase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007267" MajorTopicYN="N">Injections</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008164" MajorTopicYN="N">Luminescent Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018551" MajorTopicYN="N">Lycopersicon esculentum</DescriptorName><QualifierName UI="Q000033" MajorTopicYN="N">anatomy & histology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018613" MajorTopicYN="N">Microscopy, Confocal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010088" MajorTopicYN="N">Oxidoreductases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010957" MajorTopicYN="N">Plasmids</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012231" MajorTopicYN="N">Rhizobium</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019076" MajorTopicYN="N">Transgenes</DescriptorName><QualifierName UI="Q000502" 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Granell</name><name sortKey="Mirabel, Sophie" sort="Mirabel, Sophie" uniqKey="Mirabel S" first="Sophie" last="Mirabel">Sophie Mirabel</name><name sortKey="Wieland, Willemien H" sort="Wieland, Willemien H" uniqKey="Wieland W" first="Willemien H" last="Wieland">Willemien H. Wieland</name></noCountry><country name="Espagne"><region name="Communauté valencienne"><name sortKey="Orzaez, Diego" sort="Orzaez, Diego" uniqKey="Orzaez D" first="Diego" last="Orzaez">Diego Orzaez</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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