Transient beta-glucuronidase expression in lily (Lilium longflorum L.) pollen via wounding-assisted Agrobacterium-mediated transformation.
Identifieur interne : 000700 ( Main/Exploration ); précédent : 000699; suivant : 000701Transient beta-glucuronidase expression in lily (Lilium longflorum L.) pollen via wounding-assisted Agrobacterium-mediated transformation.
Auteurs : Su-Sung Kim [Corée du Sud] ; Dong-Il Shin ; Hee-Sung ParkSource :
- Biotechnology letters [ 0141-5492 ] ; 2007.
Descripteurs français
- KwdFr :
- ARN messager (génétique), ARN messager (métabolisme), Agrobacterium tumefaciens (croissance et développement), Agrobacterium tumefaciens (génétique), Contrainte mécanique (MeSH), Glucuronidase (génétique), Glucuronidase (métabolisme), Histocytochimie (MeSH), Lilium (enzymologie), Lilium (génétique), Lilium (microbiologie), Pollen (enzymologie), Pollen (génétique), Pollen (microbiologie), RT-PCR (MeSH), Régulation de l'expression des gènes codant pour des enzymes (MeSH), Transformation génétique (MeSH), Végétaux génétiquement modifiés (MeSH).
- MESH :
- croissance et développement : Agrobacterium tumefaciens.
- enzymologie : Lilium, Pollen.
- génétique : ARN messager, Agrobacterium tumefaciens, Glucuronidase, Lilium, Pollen.
- microbiologie : Lilium, Pollen.
- métabolisme : ARN messager, Glucuronidase.
- Contrainte mécanique, Histocytochimie, RT-PCR, Régulation de l'expression des gènes codant pour des enzymes, Transformation génétique, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Agrobacterium tumefaciens (genetics), Agrobacterium tumefaciens (growth & development), Gene Expression Regulation, Enzymologic (MeSH), Glucuronidase (genetics), Glucuronidase (metabolism), Histocytochemistry (MeSH), Lilium (enzymology), Lilium (genetics), Lilium (microbiology), Plants, Genetically Modified (MeSH), Pollen (enzymology), Pollen (genetics), Pollen (microbiology), RNA, Messenger (genetics), RNA, Messenger (metabolism), Reverse Transcriptase Polymerase Chain Reaction (MeSH), Stress, Mechanical (MeSH), Transformation, Genetic (MeSH).
- MESH :
- chemical , genetics : Glucuronidase, RNA, Messenger.
- enzymology : Lilium, Pollen.
- genetics : Agrobacterium tumefaciens, Lilium, Pollen.
- growth & development : Agrobacterium tumefaciens.
- chemical , metabolism : Glucuronidase, RNA, Messenger.
- microbiology : Lilium, Pollen.
- Gene Expression Regulation, Enzymologic, Histocytochemistry, Plants, Genetically Modified, Reverse Transcriptase Polymerase Chain Reaction, Stress, Mechanical, Transformation, Genetic.
Abstract
A pollen-based transient expression system has been developed. Lily pollen grains, wounded by vigorous shaking in the presence of aluminum oxide particles, were transformed by infiltration with Agrobacterium tumefaciens LBA4404 cells harboring the beta-glucuronidase (GUS) gene construct, pBI121. In histochemical and fluorometric GUS analysis, the wounding processes allowed efficient transformation and, in cDNA blot hybridization, GUS mRNA synthesis was clearly detected. Lily pollen with appropriate wounds, therefore, can be used conveniently for the rapid production of recombinant proteins.
DOI: 10.1007/s10529-007-9326-5
PubMed: 17310322
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Park</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2007">2007</date><idno type="RBID">pubmed:17310322</idno><idno type="pmid">17310322</idno><idno type="doi">10.1007/s10529-007-9326-5</idno><idno type="wicri:Area/Main/Corpus">000719</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000719</idno><idno type="wicri:Area/Main/Curation">000719</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000719</idno><idno type="wicri:Area/Main/Exploration">000719</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Transient beta-glucuronidase expression in lily (Lilium longflorum L.) pollen via wounding-assisted Agrobacterium-mediated transformation.</title><author><name sortKey="Kim, Su Sung" sort="Kim, Su Sung" uniqKey="Kim S" first="Su-Sung" last="Kim">Su-Sung Kim</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, Catholic University of Daegu, Kyungsan, Kyungbuk, Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Biotechnology, Catholic University of Daegu, Kyungsan, Kyungbuk</wicri:regionArea><wicri:noRegion>Kyungbuk</wicri:noRegion></affiliation></author><author><name sortKey="Shin, Dong Il" sort="Shin, Dong Il" uniqKey="Shin D" first="Dong-Il" last="Shin">Dong-Il Shin</name></author><author><name sortKey="Park, Hee Sung" sort="Park, Hee Sung" uniqKey="Park H" first="Hee-Sung" last="Park">Hee-Sung Park</name></author></analytic><series><title level="j">Biotechnology letters</title><idno type="ISSN">0141-5492</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium tumefaciens (genetics)</term><term>Agrobacterium tumefaciens (growth & development)</term><term>Gene Expression Regulation, Enzymologic (MeSH)</term><term>Glucuronidase (genetics)</term><term>Glucuronidase (metabolism)</term><term>Histocytochemistry (MeSH)</term><term>Lilium (enzymology)</term><term>Lilium (genetics)</term><term>Lilium (microbiology)</term><term>Plants, Genetically Modified (MeSH)</term><term>Pollen (enzymology)</term><term>Pollen (genetics)</term><term>Pollen (microbiology)</term><term>RNA, Messenger (genetics)</term><term>RNA, Messenger (metabolism)</term><term>Reverse Transcriptase Polymerase Chain Reaction (MeSH)</term><term>Stress, Mechanical (MeSH)</term><term>Transformation, Genetic (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN messager (génétique)</term><term>ARN messager (métabolisme)</term><term>Agrobacterium tumefaciens (croissance et développement)</term><term>Agrobacterium tumefaciens (génétique)</term><term>Contrainte mécanique (MeSH)</term><term>Glucuronidase (génétique)</term><term>Glucuronidase (métabolisme)</term><term>Histocytochimie (MeSH)</term><term>Lilium (enzymologie)</term><term>Lilium (génétique)</term><term>Lilium (microbiologie)</term><term>Pollen (enzymologie)</term><term>Pollen (génétique)</term><term>Pollen (microbiologie)</term><term>RT-PCR (MeSH)</term><term>Régulation de l'expression des gènes codant pour des enzymes (MeSH)</term><term>Transformation génétique (MeSH)</term><term>Végétaux génétiquement modifiés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Glucuronidase</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Agrobacterium tumefaciens</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Lilium</term><term>Pollen</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Lilium</term><term>Pollen</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agrobacterium tumefaciens</term><term>Lilium</term><term>Pollen</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Agrobacterium tumefaciens</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN messager</term><term>Agrobacterium tumefaciens</term><term>Glucuronidase</term><term>Lilium</term><term>Pollen</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Glucuronidase</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Lilium</term><term>Pollen</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Lilium</term><term>Pollen</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN messager</term><term>Glucuronidase</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Enzymologic</term><term>Histocytochemistry</term><term>Plants, Genetically Modified</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Stress, Mechanical</term><term>Transformation, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Contrainte mécanique</term><term>Histocytochimie</term><term>RT-PCR</term><term>Régulation de l'expression des gènes codant pour des enzymes</term><term>Transformation génétique</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A pollen-based transient expression system has been developed. Lily pollen grains, wounded by vigorous shaking in the presence of aluminum oxide particles, were transformed by infiltration with Agrobacterium tumefaciens LBA4404 cells harboring the beta-glucuronidase (GUS) gene construct, pBI121. In histochemical and fluorometric GUS analysis, the wounding processes allowed efficient transformation and, in cDNA blot hybridization, GUS mRNA synthesis was clearly detected. Lily pollen with appropriate wounds, therefore, can be used conveniently for the rapid production of recombinant proteins.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17310322</PMID><DateCompleted><Year>2007</Year><Month>08</Month><Day>27</Day></DateCompleted><DateRevised><Year>2011</Year><Month>11</Month><Day>17</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0141-5492</ISSN><JournalIssue CitedMedium="Print"><Volume>29</Volume><Issue>6</Issue><PubDate><Year>2007</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Biotechnology letters</Title><ISOAbbreviation>Biotechnol Lett</ISOAbbreviation></Journal><ArticleTitle>Transient beta-glucuronidase expression in lily (Lilium longflorum L.) pollen via wounding-assisted Agrobacterium-mediated transformation.</ArticleTitle><Pagination><MedlinePgn>965-9</MedlinePgn></Pagination><Abstract><AbstractText>A pollen-based transient expression system has been developed. Lily pollen grains, wounded by vigorous shaking in the presence of aluminum oxide particles, were transformed by infiltration with Agrobacterium tumefaciens LBA4404 cells harboring the beta-glucuronidase (GUS) gene construct, pBI121. In histochemical and fluorometric GUS analysis, the wounding processes allowed efficient transformation and, in cDNA blot hybridization, GUS mRNA synthesis was clearly detected. Lily pollen with appropriate wounds, therefore, can be used conveniently for the rapid production of recombinant proteins.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Su-Sung</ForeName><Initials>SS</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, Catholic University of Daegu, Kyungsan, Kyungbuk, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shin</LastName><ForeName>Dong-Il</ForeName><Initials>DI</Initials></Author><Author ValidYN="Y"><LastName>Park</LastName><ForeName>Hee-Sung</ForeName><Initials>HS</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2007</Year><Month>02</Month><Day>20</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Biotechnol Lett</MedlineTA><NlmUniqueID>8008051</NlmUniqueID><ISSNLinking>0141-5492</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012333">RNA, Messenger</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.31</RegistryNumber><NameOfSubstance UI="D005966">Glucuronidase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D016960" MajorTopicYN="N">Agrobacterium tumefaciens</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015971" MajorTopicYN="N">Gene Expression Regulation, Enzymologic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005966" MajorTopicYN="N">Glucuronidase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006651" MajorTopicYN="N">Histocytochemistry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D027762" MajorTopicYN="N">Lilium</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011058" MajorTopicYN="N">Pollen</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012333" MajorTopicYN="N">RNA, Messenger</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013314" MajorTopicYN="N">Stress, Mechanical</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2006</Year><Month>10</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2007</Year><Month>01</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2007</Year><Month>01</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2007</Year><Month>2</Month><Day>21</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2007</Year><Month>8</Month><Day>28</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2007</Year><Month>2</Month><Day>21</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">17310322</ArticleId><ArticleId IdType="doi">10.1007/s10529-007-9326-5</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Corée du Sud</li></country></list><tree><noCountry><name sortKey="Park, Hee Sung" sort="Park, Hee Sung" uniqKey="Park H" first="Hee-Sung" last="Park">Hee-Sung Park</name><name sortKey="Shin, Dong Il" sort="Shin, Dong Il" uniqKey="Shin D" first="Dong-Il" last="Shin">Dong-Il Shin</name></noCountry><country name="Corée du Sud"><noRegion><name sortKey="Kim, Su Sung" sort="Kim, Su Sung" uniqKey="Kim S" first="Su-Sung" last="Kim">Su-Sung Kim</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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