AgrobacTransV1, Main, Exploration, bibRecord, 000326

Transient expression using agroinfiltration to study polyadenylation in plants.

Identifieur interne : 000326 ( Main/Exploration ); précédent : 000325; suivant : 000327

Transient expression using agroinfiltration to study polyadenylation in plants.

Auteurs : Carol Von Lanken [États-Unis] ; Arthur G. Hunt

Source :

Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2015.

RBID : pubmed:25487209

Descripteurs français

KwdFr :
- ARN des plantes (métabolisme), Agrobacterium tumefaciens (croissance et développement), Agrobacterium tumefaciens (génétique), Feuilles de plante (génétique), Feuilles de plante (métabolisme), Polyadénylation (MeSH), Tabac (génétique), Tabac (microbiologie), Tabac (métabolisme), Transfection (MeSH), Transformation génétique (MeSH), Vecteurs génétiques (MeSH).
MESH :
- croissance et développement : Agrobacterium tumefaciens.
- génétique : Agrobacterium tumefaciens, Feuilles de plante, Tabac.
- microbiologie : Tabac.
- métabolisme : ARN des plantes, Feuilles de plante, Tabac.
- Polyadénylation, Transfection, Transformation génétique, Vecteurs génétiques.

English descriptors

KwdEn :
- Agrobacterium tumefaciens (genetics), Agrobacterium tumefaciens (growth & development), Genetic Vectors (MeSH), Plant Leaves (genetics), Plant Leaves (metabolism), Polyadenylation (MeSH), RNA, Plant (metabolism), Tobacco (genetics), Tobacco (metabolism), Tobacco (microbiology), Transfection (MeSH), Transformation, Genetic (MeSH).
MESH :
- chemical , metabolism : RNA, Plant.
- genetics : Agrobacterium tumefaciens, Plant Leaves, Tobacco.
- growth & development : Agrobacterium tumefaciens.
- metabolism : Plant Leaves, Tobacco.
- microbiology : Tobacco.
- Genetic Vectors, Polyadenylation, Transfection, Transformation, Genetic.

Abstract

An important facet of the study of polyadenylation in plants entails the use of transient expression to characterize poly(A) sites and the subunits of the polyadenylation complex. In this report, a simple and adaptable approach to transient expression in Nicotiana benthamiana leaves is described. This approach has been used to evaluate protein-protein interactions amongst different plant polyadenylation factor subunits and to study the usage and behavior of individual poly(A) sites from plant genes.

DOI: 10.1007/978-1-4939-2175-1_11
PubMed: 25487209

Affiliations:

États-Unis

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000368
to stream Main, to step Curation: 000368

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Transient expression using agroinfiltration to study polyadenylation in plants.</title>
<author><name sortKey="Von Lanken, Carol" sort="Von Lanken, Carol" uniqKey="Von Lanken C" first="Carol" last="Von Lanken">Carol Von Lanken</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Plant and Soil Sciences, University of Kentucky, 105 Plant Science Building, 1405 Veterans Drive, Lexington, KY, 40546, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Plant and Soil Sciences, University of Kentucky, 105 Plant Science Building, 1405 Veterans Drive, Lexington, KY, 40546</wicri:regionArea>
<wicri:noRegion>40546</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hunt, Arthur G" sort="Hunt, Arthur G" uniqKey="Hunt A" first="Arthur G" last="Hunt">Arthur G. Hunt</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2015">2015</date>
<idno type="RBID">pubmed:25487209</idno>
<idno type="pmid">25487209</idno>
<idno type="doi">10.1007/978-1-4939-2175-1_11</idno>
<idno type="wicri:Area/Main/Corpus">000368</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000368</idno>
<idno type="wicri:Area/Main/Curation">000368</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000368</idno>
<idno type="wicri:Area/Main/Exploration">000368</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Transient expression using agroinfiltration to study polyadenylation in plants.</title>
<author><name sortKey="Von Lanken, Carol" sort="Von Lanken, Carol" uniqKey="Von Lanken C" first="Carol" last="Von Lanken">Carol Von Lanken</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Plant and Soil Sciences, University of Kentucky, 105 Plant Science Building, 1405 Veterans Drive, Lexington, KY, 40546, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Plant and Soil Sciences, University of Kentucky, 105 Plant Science Building, 1405 Veterans Drive, Lexington, KY, 40546</wicri:regionArea>
<wicri:noRegion>40546</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hunt, Arthur G" sort="Hunt, Arthur G" uniqKey="Hunt A" first="Arthur G" last="Hunt">Arthur G. Hunt</name>
</author>
</analytic>
<series><title level="j">Methods in molecular biology (Clifton, N.J.)</title>
<idno type="eISSN">1940-6029</idno>
<imprint><date when="2015" type="published">2015</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium tumefaciens (genetics)</term>
<term>Agrobacterium tumefaciens (growth & development)</term>
<term>Genetic Vectors (MeSH)</term>
<term>Plant Leaves (genetics)</term>
<term>Plant Leaves (metabolism)</term>
<term>Polyadenylation (MeSH)</term>
<term>RNA, Plant (metabolism)</term>
<term>Tobacco (genetics)</term>
<term>Tobacco (metabolism)</term>
<term>Tobacco (microbiology)</term>
<term>Transfection (MeSH)</term>
<term>Transformation, Genetic (MeSH)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ARN des plantes (métabolisme)</term>
<term>Agrobacterium tumefaciens (croissance et développement)</term>
<term>Agrobacterium tumefaciens (génétique)</term>
<term>Feuilles de plante (génétique)</term>
<term>Feuilles de plante (métabolisme)</term>
<term>Polyadénylation (MeSH)</term>
<term>Tabac (génétique)</term>
<term>Tabac (microbiologie)</term>
<term>Tabac (métabolisme)</term>
<term>Transfection (MeSH)</term>
<term>Transformation génétique (MeSH)</term>
<term>Vecteurs génétiques (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>RNA, Plant</term>
</keywords>
<keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Agrobacterium tumefaciens</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agrobacterium tumefaciens</term>
<term>Plant Leaves</term>
<term>Tobacco</term>
</keywords>
<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Agrobacterium tumefaciens</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Agrobacterium tumefaciens</term>
<term>Feuilles de plante</term>
<term>Tabac</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plant Leaves</term>
<term>Tobacco</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Tabac</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Tobacco</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN des plantes</term>
<term>Feuilles de plante</term>
<term>Tabac</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Genetic Vectors</term>
<term>Polyadenylation</term>
<term>Transfection</term>
<term>Transformation, Genetic</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Polyadénylation</term>
<term>Transfection</term>
<term>Transformation génétique</term>
<term>Vecteurs génétiques</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">An important facet of the study of polyadenylation in plants entails the use of transient expression to characterize poly(A) sites and the subunits of the polyadenylation complex. In this report, a simple and adaptable approach to transient expression in Nicotiana benthamiana leaves is described. This approach has been used to evaluate protein-protein interactions amongst different plant polyadenylation factor subunits and to study the usage and behavior of individual poly(A) sites from plant genes. </div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25487209</PMID>
<DateCompleted><Year>2015</Year>
<Month>11</Month>
<Day>02</Day>
</DateCompleted>
<DateRevised><Year>2014</Year>
<Month>12</Month>
<Day>09</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1940-6029</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>1255</Volume>
<PubDate><Year>2015</Year>
</PubDate>
</JournalIssue>
<Title>Methods in molecular biology (Clifton, N.J.)</Title>
<ISOAbbreviation>Methods Mol Biol</ISOAbbreviation>
</Journal>
<ArticleTitle>Transient expression using agroinfiltration to study polyadenylation in plants.</ArticleTitle>
<Pagination><MedlinePgn>127-33</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1007/978-1-4939-2175-1_11</ELocationID>
<Abstract><AbstractText>An important facet of the study of polyadenylation in plants entails the use of transient expression to characterize poly(A) sites and the subunits of the polyadenylation complex. In this report, a simple and adaptable approach to transient expression in Nicotiana benthamiana leaves is described. This approach has been used to evaluate protein-protein interactions amongst different plant polyadenylation factor subunits and to study the usage and behavior of individual poly(A) sites from plant genes. </AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Von Lanken</LastName>
<ForeName>Carol</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>Department of Plant and Soil Sciences, University of Kentucky, 105 Plant Science Building, 1405 Veterans Drive, Lexington, KY, 40546, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hunt</LastName>
<ForeName>Arthur G</ForeName>
<Initials>AG</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>Methods Mol Biol</MedlineTA>
<NlmUniqueID>9214969</NlmUniqueID>
<ISSNLinking>1064-3745</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D018749">RNA, Plant</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D016960" MajorTopicYN="Y">Agrobacterium tumefaciens</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005822" MajorTopicYN="N">Genetic Vectors</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D026723" MajorTopicYN="N">Polyadenylation</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018749" MajorTopicYN="N">RNA, Plant</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
<QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014162" MajorTopicYN="N">Transfection</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2014</Year>
<Month>12</Month>
<Day>10</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2014</Year>
<Month>12</Month>
<Day>10</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2015</Year>
<Month>11</Month>
<Day>3</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">25487209</ArticleId>
<ArticleId IdType="doi">10.1007/978-1-4939-2175-1_11</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>États-Unis</li>
</country>
</list>
<tree><noCountry><name sortKey="Hunt, Arthur G" sort="Hunt, Arthur G" uniqKey="Hunt A" first="Arthur G" last="Hunt">Arthur G. Hunt</name>
</noCountry>
<country name="États-Unis"><noRegion><name sortKey="Von Lanken, Carol" sort="Von Lanken, Carol" uniqKey="Von Lanken C" first="Carol" last="Von Lanken">Carol Von Lanken</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Bois/explor/AgrobacTransV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000326 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000326 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Bois
   |area=    AgrobacTransV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:25487209
   |texte=   Transient expression using agroinfiltration to study polyadenylation in plants.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:25487209" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a AgrobacTransV1

This area was generated with Dilib version V0.6.38.
Data generation: Fri Nov 20 15:45:55 2020. Site generation: Wed Mar 6 15:24:41 2024

	Serveur d'exploration sur l'agrobacterium et la transgénèse
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur l'agrobacterium et la transgénèse

Transient expression using agroinfiltration to study polyadenylation in plants.

Transient expression using agroinfiltration to study polyadenylation in plants.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki