Analysis of Golgi-Mediated Protein Traffic in Plant Cells.
Identifieur interne : 000253 ( Main/Exploration ); précédent : 000252; suivant : 000254Analysis of Golgi-Mediated Protein Traffic in Plant Cells.
Auteurs : Wenjin Shen [République populaire de Chine] ; Zhidan Xiao [République populaire de Chine] ; Jinbo Shen [République populaire de Chine] ; Caiji Gao [République populaire de Chine]Source :
- Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2017.
Descripteurs français
- KwdFr :
- Agrobacterium tumefaciens (génétique), Agrobacterium tumefaciens (métabolisme), Appareil de Golgi (effets des médicaments et des substances chimiques), Appareil de Golgi (métabolisme), Appareil de Golgi (ultrastructure), Arabidopsis (effets des médicaments et des substances chimiques), Arabidopsis (génétique), Arabidopsis (métabolisme), Bréfeldine A (pharmacologie), Cellules cultivées (MeSH), Cellules végétales (effets des médicaments et des substances chimiques), Cellules végétales (métabolisme), Cellules végétales (ultrastructure), Dexaméthasone (pharmacologie), Glycosylation (effets des médicaments et des substances chimiques), Mannosyl-glycoprotéine-endo-bêta-N-acétylgluco saminidase (composition chimique), Microscopie de fluorescence (méthodes), Peptide-N4-(N-acetyl-beta-glucosaminyl) asparagine amidase (composition chimique), Plasmides (composition chimique), Plasmides (métabolisme), Protoplastes (effets des médicaments et des substances chimiques), Protoplastes (métabolisme), Protoplastes (ultrastructure), Réticulum endoplasmique (effets des médicaments et des substances chimiques), Réticulum endoplasmique (métabolisme), Réticulum endoplasmique (ultrastructure), Transfection (méthodes), Transport des protéines (effets des médicaments et des substances chimiques), Voie de sécrétion (effets des médicaments et des substances chimiques), Voie de sécrétion (génétique), Végétaux génétiquement modifiés (MeSH), Vésicules COP (effets des médicaments et des substances chimiques), Vésicules COP (métabolisme), Vésicules COP (ultrastructure), Électroporation (méthodes).
- MESH :
- composition chimique : Mannosyl-glycoprotéine-endo-bêta-N-acétylgluco saminidase, Peptide-N4-(N-acetyl-beta-glucosaminyl) asparagine amidase, Plasmides.
- effets des médicaments et des substances chimiques : Appareil de Golgi, Arabidopsis, Cellules végétales, Glycosylation, Protoplastes, Réticulum endoplasmique, Transport des protéines, Voie de sécrétion, Vésicules COP.
- génétique : Agrobacterium tumefaciens, Arabidopsis, Voie de sécrétion.
- métabolisme : Agrobacterium tumefaciens, Appareil de Golgi, Arabidopsis, Cellules végétales, Plasmides, Protoplastes, Réticulum endoplasmique, Vésicules COP.
- méthodes : Microscopie de fluorescence, Transfection, Électroporation.
- pharmacologie : Bréfeldine A, Dexaméthasone.
- ultrastructure : Appareil de Golgi, Cellules cultivées, Cellules végétales, Protoplastes, Réticulum endoplasmique, Végétaux génétiquement modifiés, Vésicules COP.
English descriptors
- KwdEn :
- Agrobacterium tumefaciens (genetics), Agrobacterium tumefaciens (metabolism), Arabidopsis (drug effects), Arabidopsis (genetics), Arabidopsis (metabolism), Brefeldin A (pharmacology), COP-Coated Vesicles (drug effects), COP-Coated Vesicles (metabolism), COP-Coated Vesicles (ultrastructure), Cells, Cultured (MeSH), Dexamethasone (pharmacology), Electroporation (methods), Endoplasmic Reticulum (drug effects), Endoplasmic Reticulum (metabolism), Endoplasmic Reticulum (ultrastructure), Glycosylation (drug effects), Golgi Apparatus (drug effects), Golgi Apparatus (metabolism), Golgi Apparatus (ultrastructure), Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase (chemistry), Microscopy, Fluorescence (methods), Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase (chemistry), Plant Cells (drug effects), Plant Cells (metabolism), Plant Cells (ultrastructure), Plants, Genetically Modified (MeSH), Plasmids (chemistry), Plasmids (metabolism), Protein Transport (drug effects), Protoplasts (drug effects), Protoplasts (metabolism), Protoplasts (ultrastructure), Secretory Pathway (drug effects), Secretory Pathway (genetics), Transfection (methods).
- MESH :
- chemical , chemistry : Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase, Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase.
- chemical , pharmacology : Brefeldin A, Dexamethasone.
- chemistry : Plasmids.
- drug effects : Arabidopsis, COP-Coated Vesicles, Endoplasmic Reticulum, Glycosylation, Golgi Apparatus, Plant Cells, Protein Transport, Protoplasts, Secretory Pathway.
- genetics : Agrobacterium tumefaciens, Arabidopsis, Secretory Pathway.
- metabolism : Agrobacterium tumefaciens, Arabidopsis, COP-Coated Vesicles, Endoplasmic Reticulum, Golgi Apparatus, Plant Cells, Plasmids, Protoplasts.
- methods : Electroporation, Microscopy, Fluorescence, Transfection.
- ultrastructure : COP-Coated Vesicles, Endoplasmic Reticulum, Golgi Apparatus, Plant Cells, Protoplasts.
- Cells, Cultured, Plants, Genetically Modified.
Abstract
In plant secretory pathways, the Golgi apparatus serves as the major sorting hub to receive de novo synthesized protein from the endoplasmic reticulum for further sorting to post-Golgi compartments or for residence in the cisternae of Golgi stacks. Meanwhile, Golgi functions as a pivotal biochemical factory to make modifications of N-glycans and to produce mature glycoproteins. Fluorescent tag-based confocal microscopy in combination with the brefeldin A drug or the genetic tools to disturb Golgi function have been shown as powerful approaches to analyze Golgi-mediated protein traffic in transiently expressed plant protoplasts or in stably expressed transgenic plants. Various endoglycosidases like Endo H and PNGase F have been widely used to monitor Golgi-mediated glycosylation of secretory proteins. Here, using fluorescently tagged Golgi-resident proteins and known glycosylated proteins as examples, we describe detailed protocols to analyze Golgi-mediated protein traffic and glycosylation in transiently expressed protoplasts derived from Arabidopsis suspension culture cells and in stably expressed transgenic plants.
DOI: 10.1007/978-1-4939-7262-3_6
PubMed: 28861818
Affiliations:
Links toward previous steps (curation, corpus...)
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of Life Sciences, South China Normal University, Guangzhou, 510631, China. gaocaiji@163.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou, 510631</wicri:regionArea><wicri:noRegion>510631</wicri:noRegion></affiliation></author></analytic><series><title level="j">Methods in molecular biology (Clifton, N.J.)</title><idno type="eISSN">1940-6029</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium tumefaciens (genetics)</term><term>Agrobacterium tumefaciens (metabolism)</term><term>Arabidopsis (drug effects)</term><term>Arabidopsis (genetics)</term><term>Arabidopsis (metabolism)</term><term>Brefeldin A (pharmacology)</term><term>COP-Coated Vesicles (drug effects)</term><term>COP-Coated Vesicles (metabolism)</term><term>COP-Coated Vesicles (ultrastructure)</term><term>Cells, Cultured (MeSH)</term><term>Dexamethasone (pharmacology)</term><term>Electroporation (methods)</term><term>Endoplasmic Reticulum (drug effects)</term><term>Endoplasmic Reticulum (metabolism)</term><term>Endoplasmic Reticulum (ultrastructure)</term><term>Glycosylation (drug effects)</term><term>Golgi Apparatus (drug effects)</term><term>Golgi Apparatus (metabolism)</term><term>Golgi Apparatus (ultrastructure)</term><term>Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase (chemistry)</term><term>Microscopy, Fluorescence (methods)</term><term>Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase (chemistry)</term><term>Plant Cells (drug effects)</term><term>Plant Cells (metabolism)</term><term>Plant Cells (ultrastructure)</term><term>Plants, Genetically Modified (MeSH)</term><term>Plasmids (chemistry)</term><term>Plasmids (metabolism)</term><term>Protein Transport (drug effects)</term><term>Protoplasts (drug effects)</term><term>Protoplasts (metabolism)</term><term>Protoplasts (ultrastructure)</term><term>Secretory Pathway (drug effects)</term><term>Secretory Pathway (genetics)</term><term>Transfection (methods)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Agrobacterium tumefaciens (génétique)</term><term>Agrobacterium tumefaciens (métabolisme)</term><term>Appareil de Golgi (effets des médicaments et des substances chimiques)</term><term>Appareil de Golgi (métabolisme)</term><term>Appareil de Golgi (ultrastructure)</term><term>Arabidopsis (effets des médicaments et des substances chimiques)</term><term>Arabidopsis (génétique)</term><term>Arabidopsis (métabolisme)</term><term>Bréfeldine A (pharmacologie)</term><term>Cellules cultivées (MeSH)</term><term>Cellules végétales (effets des médicaments et des substances chimiques)</term><term>Cellules végétales (métabolisme)</term><term>Cellules végétales (ultrastructure)</term><term>Dexaméthasone (pharmacologie)</term><term>Glycosylation (effets des médicaments et des substances chimiques)</term><term>Mannosyl-glycoprotéine-endo-bêta-N-acétylgluco saminidase (composition chimique)</term><term>Microscopie de fluorescence (méthodes)</term><term>Peptide-N4-(N-acetyl-beta-glucosaminyl) asparagine amidase (composition chimique)</term><term>Plasmides (composition chimique)</term><term>Plasmides (métabolisme)</term><term>Protoplastes (effets des médicaments et des substances chimiques)</term><term>Protoplastes (métabolisme)</term><term>Protoplastes (ultrastructure)</term><term>Réticulum endoplasmique (effets des médicaments et des substances chimiques)</term><term>Réticulum endoplasmique (métabolisme)</term><term>Réticulum endoplasmique (ultrastructure)</term><term>Transfection (méthodes)</term><term>Transport des protéines (effets des médicaments et des substances chimiques)</term><term>Voie de sécrétion (effets des médicaments et des substances chimiques)</term><term>Voie de sécrétion (génétique)</term><term>Végétaux génétiquement modifiés (MeSH)</term><term>Vésicules COP (effets des médicaments et des substances chimiques)</term><term>Vésicules COP (métabolisme)</term><term>Vésicules COP (ultrastructure)</term><term>Électroporation (méthodes)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase</term><term>Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Brefeldin A</term><term>Dexamethasone</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Plasmids</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Mannosyl-glycoprotéine-endo-bêta-N-acétylgluco saminidase</term><term>Peptide-N4-(N-acetyl-beta-glucosaminyl) asparagine 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tumefaciens</term><term>Arabidopsis</term><term>Voie de sécrétion</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Agrobacterium tumefaciens</term><term>Arabidopsis</term><term>COP-Coated Vesicles</term><term>Endoplasmic Reticulum</term><term>Golgi Apparatus</term><term>Plant Cells</term><term>Plasmids</term><term>Protoplasts</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Electroporation</term><term>Microscopy, Fluorescence</term><term>Transfection</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Agrobacterium tumefaciens</term><term>Appareil de Golgi</term><term>Arabidopsis</term><term>Cellules végétales</term><term>Plasmides</term><term>Protoplastes</term><term>Réticulum endoplasmique</term><term>Vésicules COP</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Microscopie de fluorescence</term><term>Transfection</term><term>Électroporation</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Bréfeldine A</term><term>Dexaméthasone</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>COP-Coated Vesicles</term><term>Endoplasmic Reticulum</term><term>Golgi Apparatus</term><term>Plant Cells</term><term>Protoplasts</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cells, Cultured</term><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="fr"><term>Appareil de Golgi</term><term>Cellules cultivées</term><term>Cellules végétales</term><term>Protoplastes</term><term>Réticulum endoplasmique</term><term>Végétaux génétiquement modifiés</term><term>Vésicules COP</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In plant secretory pathways, the Golgi apparatus serves as the major sorting hub to receive de novo synthesized protein from the endoplasmic reticulum for further sorting to post-Golgi compartments or for residence in the cisternae of Golgi stacks. Meanwhile, Golgi functions as a pivotal biochemical factory to make modifications of N-glycans and to produce mature glycoproteins. Fluorescent tag-based confocal microscopy in combination with the brefeldin A drug or the genetic tools to disturb Golgi function have been shown as powerful approaches to analyze Golgi-mediated protein traffic in transiently expressed plant protoplasts or in stably expressed transgenic plants. Various endoglycosidases like Endo H and PNGase F have been widely used to monitor Golgi-mediated glycosylation of secretory proteins. Here, using fluorescently tagged Golgi-resident proteins and known glycosylated proteins as examples, we describe detailed protocols to analyze Golgi-mediated protein traffic and glycosylation in transiently expressed protoplasts derived from Arabidopsis suspension culture cells and in stably expressed transgenic plants.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28861818</PMID><DateCompleted><Year>2018</Year><Month>05</Month><Day>15</Day></DateCompleted><DateRevised><Year>2018</Year><Month>08</Month><Day>10</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1940-6029</ISSN><JournalIssue CitedMedium="Internet"><Volume>1662</Volume><PubDate><Year>2017</Year></PubDate></JournalIssue><Title>Methods in molecular biology (Clifton, N.J.)</Title><ISOAbbreviation>Methods Mol Biol</ISOAbbreviation></Journal><ArticleTitle>Analysis of Golgi-Mediated Protein Traffic in Plant Cells.</ArticleTitle><Pagination><MedlinePgn>75-86</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/978-1-4939-7262-3_6</ELocationID><Abstract><AbstractText>In plant secretory pathways, the Golgi apparatus serves as the major sorting hub to receive de novo synthesized protein from the endoplasmic reticulum for further sorting to post-Golgi compartments or for residence in the cisternae of Golgi stacks. Meanwhile, Golgi functions as a pivotal biochemical factory to make modifications of N-glycans and to produce mature glycoproteins. Fluorescent tag-based confocal microscopy in combination with the brefeldin A drug or the genetic tools to disturb Golgi function have been shown as powerful approaches to analyze Golgi-mediated protein traffic in transiently expressed plant protoplasts or in stably expressed transgenic plants. Various endoglycosidases like Endo H and PNGase F have been widely used to monitor Golgi-mediated glycosylation of secretory proteins. Here, using fluorescently tagged Golgi-resident proteins and known glycosylated proteins as examples, we describe detailed protocols to analyze Golgi-mediated protein traffic and glycosylation in transiently expressed protoplasts derived from Arabidopsis suspension culture cells and in stably expressed transgenic plants.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Shen</LastName><ForeName>Wenjin</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou, 510631, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xiao</LastName><ForeName>Zhidan</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou, 510631, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shen</LastName><ForeName>Jinbo</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Agrobiotechnology, Centre for Cell and Developmental Biology, School of Life Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gao</LastName><ForeName>Caiji</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou, 510631, China. gaocaiji@163.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Methods Mol Biol</MedlineTA><NlmUniqueID>9214969</NlmUniqueID><ISSNLinking>1064-3745</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>20350-15-6</RegistryNumber><NameOfSubstance UI="D020126">Brefeldin A</NameOfSubstance></Chemical><Chemical><RegistryNumber>7S5I7G3JQL</RegistryNumber><NameOfSubstance UI="D003907">Dexamethasone</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.96</RegistryNumber><NameOfSubstance UI="D017038">Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.5.1.52</RegistryNumber><NameOfSubstance UI="D043524">Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D016960" MajorTopicYN="N">Agrobacterium tumefaciens</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017360" MajorTopicYN="N">Arabidopsis</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020126" MajorTopicYN="N">Brefeldin A</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D022181" MajorTopicYN="N">COP-Coated Vesicles</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002478" MajorTopicYN="N">Cells, Cultured</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003907" MajorTopicYN="N">Dexamethasone</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018274" MajorTopicYN="N">Electroporation</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004721" MajorTopicYN="N">Endoplasmic Reticulum</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006031" MajorTopicYN="N">Glycosylation</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006056" MajorTopicYN="N">Golgi Apparatus</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017038" MajorTopicYN="N">Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008856" MajorTopicYN="N">Microscopy, Fluorescence</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D043524" MajorTopicYN="N">Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D059828" MajorTopicYN="N">Plant Cells</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010957" MajorTopicYN="N">Plasmids</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D021381" MajorTopicYN="N">Protein Transport</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011523" MajorTopicYN="N">Protoplasts</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D055571" MajorTopicYN="N">Secretory Pathway</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014162" MajorTopicYN="N">Transfection</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Arabidopsis</Keyword><Keyword MajorTopicYN="Y">COPI vesicle</Keyword><Keyword MajorTopicYN="Y">Confocal microscopy</Keyword><Keyword MajorTopicYN="Y">Endoglycosidase</Keyword><Keyword MajorTopicYN="Y">Golgi</Keyword><Keyword MajorTopicYN="Y">N-glycosylation</Keyword><Keyword MajorTopicYN="Y">Protoplast</Keyword><Keyword MajorTopicYN="Y">Transient expression</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>9</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>9</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>5</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">28861818</ArticleId><ArticleId IdType="doi">10.1007/978-1-4939-7262-3_6</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Sha Tin</li></settlement><orgName><li>Université chinoise de Hong Kong</li></orgName></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Shen, Wenjin" sort="Shen, Wenjin" uniqKey="Shen W" first="Wenjin" last="Shen">Wenjin Shen</name></noRegion><name sortKey="Gao, Caiji" sort="Gao, Caiji" uniqKey="Gao C" first="Caiji" last="Gao">Caiji Gao</name><name sortKey="Shen, Jinbo" sort="Shen, Jinbo" uniqKey="Shen J" first="Jinbo" last="Shen">Jinbo Shen</name><name sortKey="Xiao, Zhidan" sort="Xiao, Zhidan" uniqKey="Xiao Z" first="Zhidan" last="Xiao">Zhidan Xiao</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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{{Explor lien
|wiki= Bois
|area= AgrobacTransV1
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|texte= Analysis of Golgi-Mediated Protein Traffic in Plant Cells.
}}
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