AgrobacTransV1, Main, Exploration, bibRecord, 000121

Efficient transient protein expression in tomato cultivars and wild species using agroinfiltration-mediated high expression system.

Identifieur interne : 000121 ( Main/Exploration ); précédent : 000120; suivant : 000122

Efficient transient protein expression in tomato cultivars and wild species using agroinfiltration-mediated high expression system.

Auteurs : Ken Hoshikawa [Japon] ; Satoshi Fujita [Japon] ; Na Renhu [Japon] ; Kentaro Ezura [Japon] ; Tsuyoshi Yamamoto [Japon] ; Satoko Nonaka [Japon] ; Hiroshi Ezura [Japon] ; Kenji Miura [Japon]

Source :

Plant cell reports [ 1432-203X ] ; 2019.

RBID : pubmed:30328507

Descripteurs français

English descriptors

KwdEn :
- 4-Aminobutyrate Transaminase (genetics), 4-Aminobutyrate Transaminase (metabolism), Agrobacterium (genetics), Agrobacterium (metabolism), Gene Expression Regulation, Plant (MeSH), Lycopersicon esculentum (genetics), Lycopersicon esculentum (metabolism), Lycopersicon esculentum (microbiology), Plant Proteins (genetics), Plant Proteins (metabolism), Plants, Genetically Modified (genetics), Plants, Genetically Modified (metabolism).
MESH :
- chemical , genetics : 4-Aminobutyrate Transaminase, Plant Proteins.
- chemical , metabolism : 4-Aminobutyrate Transaminase, Plant Proteins.
- genetics : Agrobacterium, Lycopersicon esculentum, Plants, Genetically Modified.
- metabolism : Agrobacterium, Lycopersicon esculentum, Plants, Genetically Modified.
- microbiology : Lycopersicon esculentum.
- Gene Expression Regulation, Plant.

Abstract

KEY MESSAGE

The new transient protein expression system using the pBYR2HS vector is applicable to several tomato cultivars and wild species with high level of protein expression. Innovation and improvement of effective tools for transient protein expression in plant cells is critical for the development of plant biotechnology. We have created the new transient protein expression system using the pBYR2HS vector that led to about 4 mg/g fresh weight of protein expression in Nicotiana benthamiana. In this study, we validated the adaptability of this transient protein expression system by agroinfiltration to leaves and fruits of several tomato cultivars and wild species. Although the GFP protein was transiently expressed in the leaves and fruits of all tomato cultivars and wild species, we observed species-specific differences in protein expression. In particular, GFP protein expression was higher in the leaves and fruits of Micro-Tom, Solanum pimpinellifolium (0043) and S. pimpinellifolium (0049-w1) than in those of cultivars and wild species. Furthermore, Agrobacterium with GABA transaminase enhanced transient expression in tomato fruits of Micro-Tom. Taken together with these results, our system is applicable to several tomato cultivars and species as well as a model tomato, even though characteristics are often different among tomato cultivars or species. Thus, the system is an effective, simple, and valuable tool to achieve rapid transgene expression to examine gene function in tomato plant cells.

DOI: 10.1007/s00299-018-2350-1
PubMed: 30328507

Affiliations:

Japon

Links toward previous steps (curation, corpus...)

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Le document en format XML

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<term>Agrobacterium (genetics)</term>
<term>Agrobacterium (metabolism)</term>
<term>Gene Expression Regulation, Plant (MeSH)</term>
<term>Lycopersicon esculentum (genetics)</term>
<term>Lycopersicon esculentum (metabolism)</term>
<term>Lycopersicon esculentum (microbiology)</term>
<term>Plant Proteins (genetics)</term>
<term>Plant Proteins (metabolism)</term>
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<term>Plants, Genetically Modified (metabolism)</term>
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<term>Agrobacterium (génétique)</term>
<term>Agrobacterium (métabolisme)</term>
<term>Lycopersicon esculentum (génétique)</term>
<term>Lycopersicon esculentum (microbiologie)</term>
<term>Lycopersicon esculentum (métabolisme)</term>
<term>Protéines végétales (génétique)</term>
<term>Protéines végétales (métabolisme)</term>
<term>Régulation de l'expression des gènes végétaux (MeSH)</term>
<term>Végétaux génétiquement modifiés (génétique)</term>
<term>Végétaux génétiquement modifiés (métabolisme)</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>4-Aminobutyrate Transaminase</term>
<term>Plant Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>4-Aminobutyrate Transaminase</term>
<term>Plant Proteins</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agrobacterium</term>
<term>Lycopersicon esculentum</term>
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<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>4-Aminobutyrate transaminase</term>
<term>Agrobacterium</term>
<term>Lycopersicon esculentum</term>
<term>Protéines végétales</term>
<term>Végétaux génétiquement modifiés</term>
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<term>Lycopersicon esculentum</term>
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<term>Agrobacterium</term>
<term>Lycopersicon esculentum</term>
<term>Protéines végétales</term>
<term>Végétaux génétiquement modifiés</term>
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<p>The new transient protein expression system using the pBYR2HS vector is applicable to several tomato cultivars and wild species with high level of protein expression. Innovation and improvement of effective tools for transient protein expression in plant cells is critical for the development of plant biotechnology. We have created the new transient protein expression system using the pBYR2HS vector that led to about 4 mg/g fresh weight of protein expression in Nicotiana benthamiana. In this study, we validated the adaptability of this transient protein expression system by agroinfiltration to leaves and fruits of several tomato cultivars and wild species. Although the GFP protein was transiently expressed in the leaves and fruits of all tomato cultivars and wild species, we observed species-specific differences in protein expression. In particular, GFP protein expression was higher in the leaves and fruits of Micro-Tom, Solanum pimpinellifolium (0043) and S. pimpinellifolium (0049-w1) than in those of cultivars and wild species. Furthermore, Agrobacterium with GABA transaminase enhanced transient expression in tomato fruits of Micro-Tom. Taken together with these results, our system is applicable to several tomato cultivars and species as well as a model tomato, even though characteristics are often different among tomato cultivars or species. Thus, the system is an effective, simple, and valuable tool to achieve rapid transgene expression to examine gene function in tomato plant cells.</p>
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<Abstract><AbstractText Label="KEY MESSAGE" NlmCategory="UNASSIGNED">The new transient protein expression system using the pBYR2HS vector is applicable to several tomato cultivars and wild species with high level of protein expression. Innovation and improvement of effective tools for transient protein expression in plant cells is critical for the development of plant biotechnology. We have created the new transient protein expression system using the pBYR2HS vector that led to about 4 mg/g fresh weight of protein expression in Nicotiana benthamiana. In this study, we validated the adaptability of this transient protein expression system by agroinfiltration to leaves and fruits of several tomato cultivars and wild species. Although the GFP protein was transiently expressed in the leaves and fruits of all tomato cultivars and wild species, we observed species-specific differences in protein expression. In particular, GFP protein expression was higher in the leaves and fruits of Micro-Tom, Solanum pimpinellifolium (0043) and S. pimpinellifolium (0049-w1) than in those of cultivars and wild species. Furthermore, Agrobacterium with GABA transaminase enhanced transient expression in tomato fruits of Micro-Tom. Taken together with these results, our system is applicable to several tomato cultivars and species as well as a model tomato, even though characteristics are often different among tomato cultivars or species. Thus, the system is an effective, simple, and valuable tool to achieve rapid transgene expression to examine gene function in tomato plant cells.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hoshikawa</LastName>
<ForeName>Ken</ForeName>
<Initials>K</Initials>
<AffiliationInfo><Affiliation>Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8572, Japan.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Japan International Research Center for Agricultural Sciences, 1-1 Ohwashi, Tsukuba, Ibaraki, 305-8686, Japan.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Fujita</LastName>
<ForeName>Satoshi</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8572, Japan.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Renhu</LastName>
<ForeName>Na</ForeName>
<Initials>N</Initials>
<AffiliationInfo><Affiliation>Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8572, Japan.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ezura</LastName>
<ForeName>Kentaro</ForeName>
<Initials>K</Initials>
<AffiliationInfo><Affiliation>Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8572, Japan.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Yamamoto</LastName>
<ForeName>Tsuyoshi</ForeName>
<Initials>T</Initials>
<AffiliationInfo><Affiliation>Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8572, Japan.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Nonaka</LastName>
<ForeName>Satoko</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8572, Japan.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Tsukuba-Plant Innovation Research Center, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8572, Japan.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ezura</LastName>
<ForeName>Hiroshi</ForeName>
<Initials>H</Initials>
<AffiliationInfo><Affiliation>Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8572, Japan. ezura.hiroshi.fa@u.tsukuba.ac.jp.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Tsukuba-Plant Innovation Research Center, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8572, Japan. ezura.hiroshi.fa@u.tsukuba.ac.jp.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Miura</LastName>
<ForeName>Kenji</ForeName>
<Initials>K</Initials>
<Identifier Source="ORCID">http://orcid.org/0000-0003-1262-2176</Identifier>
<AffiliationInfo><Affiliation>Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8572, Japan. miura.kenji.ga@u.tsukuba.ac.jp.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Tsukuba-Plant Innovation Research Center, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8572, Japan. miura.kenji.ga@u.tsukuba.ac.jp.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<GrantList CompleteYN="Y"><Grant><GrantID>JP16K07390</GrantID>
<Agency>Japan Society for the Promotion of Science</Agency>
<Country></Country>
</Grant>
</GrantList>
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</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2018</Year>
<Month>10</Month>
<Day>17</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>Germany</Country>
<MedlineTA>Plant Cell Rep</MedlineTA>
<NlmUniqueID>9880970</NlmUniqueID>
<ISSNLinking>0721-7714</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 2.6.1.19</RegistryNumber>
<NameOfSubstance UI="D000612">4-Aminobutyrate Transaminase</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000612" MajorTopicYN="N">4-Aminobutyrate Transaminase</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D060054" MajorTopicYN="N">Agrobacterium</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018551" MajorTopicYN="N">Lycopersicon esculentum</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
<QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName>
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<MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Agroinfiltration</Keyword>
<Keyword MajorTopicYN="N">Tomato</Keyword>
<Keyword MajorTopicYN="N">Transient protein expression</Keyword>
</KeywordList>
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<Month>09</Month>
<Day>19</Day>
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<Day>07</Day>
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<Day>18</Day>
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<tree><country name="Japon"><noRegion><name sortKey="Hoshikawa, Ken" sort="Hoshikawa, Ken" uniqKey="Hoshikawa K" first="Ken" last="Hoshikawa">Ken Hoshikawa</name>
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<name sortKey="Ezura, Hiroshi" sort="Ezura, Hiroshi" uniqKey="Ezura H" first="Hiroshi" last="Ezura">Hiroshi Ezura</name>
<name sortKey="Ezura, Hiroshi" sort="Ezura, Hiroshi" uniqKey="Ezura H" first="Hiroshi" last="Ezura">Hiroshi Ezura</name>
<name sortKey="Ezura, Kentaro" sort="Ezura, Kentaro" uniqKey="Ezura K" first="Kentaro" last="Ezura">Kentaro Ezura</name>
<name sortKey="Fujita, Satoshi" sort="Fujita, Satoshi" uniqKey="Fujita S" first="Satoshi" last="Fujita">Satoshi Fujita</name>
<name sortKey="Hoshikawa, Ken" sort="Hoshikawa, Ken" uniqKey="Hoshikawa K" first="Ken" last="Hoshikawa">Ken Hoshikawa</name>
<name sortKey="Miura, Kenji" sort="Miura, Kenji" uniqKey="Miura K" first="Kenji" last="Miura">Kenji Miura</name>
<name sortKey="Miura, Kenji" sort="Miura, Kenji" uniqKey="Miura K" first="Kenji" last="Miura">Kenji Miura</name>
<name sortKey="Nonaka, Satoko" sort="Nonaka, Satoko" uniqKey="Nonaka S" first="Satoko" last="Nonaka">Satoko Nonaka</name>
<name sortKey="Nonaka, Satoko" sort="Nonaka, Satoko" uniqKey="Nonaka S" first="Satoko" last="Nonaka">Satoko Nonaka</name>
<name sortKey="Renhu, Na" sort="Renhu, Na" uniqKey="Renhu N" first="Na" last="Renhu">Na Renhu</name>
<name sortKey="Yamamoto, Tsuyoshi" sort="Yamamoto, Tsuyoshi" uniqKey="Yamamoto T" first="Tsuyoshi" last="Yamamoto">Tsuyoshi Yamamoto</name>
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	Serveur d'exploration sur l'agrobacterium et la transgénèse
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Efficient transient protein expression in tomato cultivars and wild species using agroinfiltration-mediated high expression system.

Efficient transient protein expression in tomato cultivars and wild species using agroinfiltration-mediated high expression system.

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