Resistance analysis of cherry rootstock 'CDR-1' (Prunus mahaleb) to crown gall disease.
Identifieur interne : 000022 ( Main/Exploration ); précédent : 000021; suivant : 000023Resistance analysis of cherry rootstock 'CDR-1' (Prunus mahaleb) to crown gall disease.
Auteurs : Chenglin Liang [République populaire de Chine] ; Tian Wan [République populaire de Chine] ; Rendun Wu [République populaire de Chine] ; Mei Zhao [République populaire de Chine] ; Yue Zhao [République populaire de Chine] ; Yuliang Cai [République populaire de Chine]Source :
- BMC plant biology [ 1471-2229 ] ; 2020.
Abstract
BACKGROUND
Crown gall disease, caused by the pathogenic bacterium Agrobacterium tumefaciens, is responsible for extensive economic losses in orchards. Cherry rootstock 'CDR-1' (Prunus mahaleb) shows high resistance but the mechanism remains unclear. Here, we examined the morphology of pathogen-infected root neck surface, determined the activity of 10 defense-related enzymes and the content of salicylic acid (SA) and jasmonic acid (JA), and also applied transcriptome analysis, transient expression and transgenic verification to explore the crown gall resistance genes in 'CDR-1' plants.
RESULTS
In our study, peroxidase increased in the first 10 days, while phenylalanine ammonialyase and lipoxygenase increased in the first 15 days post-infection. Four key enzymes in the AsA-GSH cycle also responded, to a certain extent; although JA content increased significantly after the treatment, the SA content did not. In a follow-up transcriptome analysis, the differentially expressed genes Pm4CL2, PmCYP450, PmHCT1, PmHCT2, and PmCAD were up-regulated. Based on the above results, we focused on the lignin biosynthetic pathway, and further measured lignin content, and found it increased significantly. The Pm4CL2 gene was used to conduct transient expression and transgenic experiments to verify its function in crown gall disease resistance. It showed the relative expression of the treatment group was almost 14-fold that of the control group at 12 h post-treatment. After the infection treatment, clear signs of resistance were found in the transgenic lines; this indicated that under the higher expression level and earlier activation of Pm4CL2, plant resistance was enhanced.
CONCLUSIONS
The crown gall resistance of 'CDR-1' is likely related to the lignin biosynthetic pathway, in which Pm4CL2 functions crucially during the plant defense response to the pathogen A. tumefaciens. The results thus offer novel insights into the defense responses and resistance mechanism of cherry rootstock 'CDR-1' against crown gall disease.
DOI: 10.1186/s12870-020-02673-0
PubMed: 33183241
PubMed Central: PMC7661173
Affiliations:
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China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi Province</wicri:regionArea><wicri:noRegion>Shaanxi Province</wicri:noRegion></affiliation></author><author><name sortKey="Wu, Rendun" sort="Wu, Rendun" uniqKey="Wu R" first="Rendun" last="Wu">Rendun Wu</name><affiliation wicri:level="1"><nlm:affiliation>College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi Province, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi Province</wicri:regionArea><wicri:noRegion>Shaanxi Province</wicri:noRegion></affiliation></author><author><name sortKey="Zhao, Mei" sort="Zhao, Mei" uniqKey="Zhao M" first="Mei" last="Zhao">Mei Zhao</name><affiliation wicri:level="1"><nlm:affiliation>College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi Province, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi Province</wicri:regionArea><wicri:noRegion>Shaanxi Province</wicri:noRegion></affiliation></author><author><name sortKey="Zhao, Yue" sort="Zhao, Yue" uniqKey="Zhao Y" first="Yue" last="Zhao">Yue Zhao</name><affiliation wicri:level="1"><nlm:affiliation>College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi Province, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi Province</wicri:regionArea><wicri:noRegion>Shaanxi Province</wicri:noRegion></affiliation></author><author><name sortKey="Cai, Yuliang" sort="Cai, Yuliang" uniqKey="Cai Y" first="Yuliang" last="Cai">Yuliang Cai</name><affiliation wicri:level="1"><nlm:affiliation>College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi Province, China. yuanyicyl@nwsuaf.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi Province</wicri:regionArea><wicri:noRegion>Shaanxi Province</wicri:noRegion></affiliation></author></analytic><series><title level="j">BMC plant biology</title><idno type="eISSN">1471-2229</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>Crown gall disease, caused by the pathogenic bacterium Agrobacterium tumefaciens, is responsible for extensive economic losses in orchards. Cherry rootstock 'CDR-1' (Prunus mahaleb) shows high resistance but the mechanism remains unclear. Here, we examined the morphology of pathogen-infected root neck surface, determined the activity of 10 defense-related enzymes and the content of salicylic acid (SA) and jasmonic acid (JA), and also applied transcriptome analysis, transient expression and transgenic verification to explore the crown gall resistance genes in 'CDR-1' plants.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>In our study, peroxidase increased in the first 10 days, while phenylalanine ammonialyase and lipoxygenase increased in the first 15 days post-infection. Four key enzymes in the AsA-GSH cycle also responded, to a certain extent; although JA content increased significantly after the treatment, the SA content did not. In a follow-up transcriptome analysis, the differentially expressed genes Pm4CL2, PmCYP450, PmHCT1, PmHCT2, and PmCAD were up-regulated. Based on the above results, we focused on the lignin biosynthetic pathway, and further measured lignin content, and found it increased significantly. The Pm4CL2 gene was used to conduct transient expression and transgenic experiments to verify its function in crown gall disease resistance. It showed the relative expression of the treatment group was almost 14-fold that of the control group at 12 h post-treatment. After the infection treatment, clear signs of resistance were found in the transgenic lines; this indicated that under the higher expression level and earlier activation of Pm4CL2, plant resistance was enhanced.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>The crown gall resistance of 'CDR-1' is likely related to the lignin biosynthetic pathway, in which Pm4CL2 functions crucially during the plant defense response to the pathogen A. tumefaciens. The results thus offer novel insights into the defense responses and resistance mechanism of cherry rootstock 'CDR-1' against crown gall disease.</p></div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">33183241</PMID><DateRevised><Year>2020</Year><Month>11</Month><Day>19</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1471-2229</ISSN><JournalIssue CitedMedium="Internet"><Volume>20</Volume><Issue>1</Issue><PubDate><Year>2020</Year><Month>Nov</Month><Day>12</Day></PubDate></JournalIssue><Title>BMC plant biology</Title><ISOAbbreviation>BMC Plant Biol</ISOAbbreviation></Journal><ArticleTitle>Resistance analysis of cherry rootstock 'CDR-1' (Prunus mahaleb) to crown gall disease.</ArticleTitle><Pagination><MedlinePgn>516</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/s12870-020-02673-0</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Crown gall disease, caused by the pathogenic bacterium Agrobacterium tumefaciens, is responsible for extensive economic losses in orchards. Cherry rootstock 'CDR-1' (Prunus mahaleb) shows high resistance but the mechanism remains unclear. Here, we examined the morphology of pathogen-infected root neck surface, determined the activity of 10 defense-related enzymes and the content of salicylic acid (SA) and jasmonic acid (JA), and also applied transcriptome analysis, transient expression and transgenic verification to explore the crown gall resistance genes in 'CDR-1' plants.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">In our study, peroxidase increased in the first 10 days, while phenylalanine ammonialyase and lipoxygenase increased in the first 15 days post-infection. Four key enzymes in the AsA-GSH cycle also responded, to a certain extent; although JA content increased significantly after the treatment, the SA content did not. In a follow-up transcriptome analysis, the differentially expressed genes Pm4CL2, PmCYP450, PmHCT1, PmHCT2, and PmCAD were up-regulated. Based on the above results, we focused on the lignin biosynthetic pathway, and further measured lignin content, and found it increased significantly. The Pm4CL2 gene was used to conduct transient expression and transgenic experiments to verify its function in crown gall disease resistance. It showed the relative expression of the treatment group was almost 14-fold that of the control group at 12 h post-treatment. After the infection treatment, clear signs of resistance were found in the transgenic lines; this indicated that under the higher expression level and earlier activation of Pm4CL2, plant resistance was enhanced.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">The crown gall resistance of 'CDR-1' is likely related to the lignin biosynthetic pathway, in which Pm4CL2 functions crucially during the plant defense response to the pathogen A. tumefaciens. The results thus offer novel insights into the defense responses and resistance mechanism of cherry rootstock 'CDR-1' against crown gall disease.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Liang</LastName><ForeName>Chenglin</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi Province, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wan</LastName><ForeName>Tian</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi Province, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Rendun</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi Province, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Mei</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi Province, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Yue</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi Province, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cai</LastName><ForeName>Yuliang</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi Province, China. yuanyicyl@nwsuaf.edu.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>11</Month><Day>12</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>BMC Plant Biol</MedlineTA><NlmUniqueID>100967807</NlmUniqueID><ISSNLinking>1471-2229</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">A. tumefaciens</Keyword><Keyword MajorTopicYN="N">Crown gall disease</Keyword><Keyword MajorTopicYN="N">Gene function</Keyword><Keyword MajorTopicYN="N">Lignin biosynthetic pathway</Keyword><Keyword MajorTopicYN="N">Pm4CL2</Keyword><Keyword MajorTopicYN="N">Resistance</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>06</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>09</Month><Day>23</Day></PubMedPubDate><PubMedPubDate 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last="Liang">Chenglin Liang</name></noRegion><name sortKey="Cai, Yuliang" sort="Cai, Yuliang" uniqKey="Cai Y" first="Yuliang" last="Cai">Yuliang Cai</name><name sortKey="Wan, Tian" sort="Wan, Tian" uniqKey="Wan T" first="Tian" last="Wan">Tian Wan</name><name sortKey="Wu, Rendun" sort="Wu, Rendun" uniqKey="Wu R" first="Rendun" last="Wu">Rendun Wu</name><name sortKey="Zhao, Mei" sort="Zhao, Mei" uniqKey="Zhao M" first="Mei" last="Zhao">Mei Zhao</name><name sortKey="Zhao, Yue" sort="Zhao, Yue" uniqKey="Zhao Y" first="Yue" last="Zhao">Yue Zhao</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:33183241" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a AgrobacTransV1
| This area was generated with Dilib version V0.6.38. |  |