Transformation improvement with the Standardized Pressure Agrobacterium Infiltration Device (SPAID).
Identifieur interne : 000117 ( Main/Curation ); précédent : 000116; suivant : 000118Transformation improvement with the Standardized Pressure Agrobacterium Infiltration Device (SPAID).
Auteurs : Mohamad Fadhli Bin Mad' Atari [États-Unis, Malaisie] ; Kevin M. Folta [États-Unis]Source :
- BMC research notes [ 1756-0500 ] ; 2019.
Descripteurs français
- KwdFr :
- Agrobacterium tumefaciens (génétique), Arabidopsis (génétique), Feuilles de plante (génétique), Fragaria (génétique), Génie génétique (instrumentation), Génie génétique (méthodes), Reproductibilité des résultats (MeSH), Tabac (génétique), Transformation génétique (MeSH), Végétaux génétiquement modifiés (MeSH).
- MESH :
- génétique : Agrobacterium tumefaciens, Arabidopsis, Feuilles de plante, Fragaria, Tabac.
- méthodes : Génie génétique.
- instrumentation : Génie génétique, Reproductibilité des résultats, Transformation génétique, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Agrobacterium tumefaciens (genetics), Arabidopsis (genetics), Fragaria (genetics), Genetic Engineering (instrumentation), Genetic Engineering (methods), Plant Leaves (genetics), Plants, Genetically Modified (MeSH), Reproducibility of Results (MeSH), Tobacco (genetics), Transformation, Genetic (MeSH).
- MESH :
- genetics : Agrobacterium tumefaciens, Arabidopsis, Fragaria, Plant Leaves, Tobacco.
- instrumentation : Genetic Engineering.
- methods : Genetic Engineering.
- Plants, Genetically Modified, Reproducibility of Results, Transformation, Genetic.
Abstract
OBJECTIVE
The treatment of plant tissue with Agrobacterium tumefaciens is often a critical first step to both stable and transient plant transformation. In both applications bacterial suspensions are oftentimes physically introduced into plant tissues using hand-driven pressure from a needleless syringe. While effective, this approach has several drawbacks that limit reproducibility. Pressure must be provided with the syringe perfectly perpendicular to the tissue surface. The researcher must also attempt to provide even and consistent pressure, both within and between experimental replicates. These factors mean that the procedures do not always translate well between research groups or biological replicates.
RESULTS
We have devised a method to introduce Agrobacterium suspensions into plant leaves with greater reproducibility. Using a decommissioned dissecting microscope as an armature, a syringe body with the bacterial suspension is mounted to the nosepiece. Gentle, even pressure is applied by rotating the focus knob. The treatment force is measured using a basic kitchen scale. The development of the Standardized Pressure Agrobacterium Infiltration Device (SPAID) provides a means to deliver consistent amounts of bacterial suspensions into plant tissues with the goal of increasing reproducibility between replicates and laboratories.
DOI: 10.1186/s13104-019-4117-3
PubMed: 30876440
PubMed Central: PMC6419847
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pubmed:30876440Le document en format XML
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<author><name sortKey="Mad Atari, Mohamad Fadhli Bin" sort="Mad Atari, Mohamad Fadhli Bin" uniqKey="Mad Atari M" first="Mohamad Fadhli Bin" last="Mad' Atari">Mohamad Fadhli Bin Mad' Atari</name>
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<term>Arabidopsis (genetics)</term>
<term>Fragaria (genetics)</term>
<term>Genetic Engineering (instrumentation)</term>
<term>Genetic Engineering (methods)</term>
<term>Plant Leaves (genetics)</term>
<term>Plants, Genetically Modified (MeSH)</term>
<term>Reproducibility of Results (MeSH)</term>
<term>Tobacco (genetics)</term>
<term>Transformation, Genetic (MeSH)</term>
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<term>Fragaria (génétique)</term>
<term>Génie génétique (instrumentation)</term>
<term>Génie génétique (méthodes)</term>
<term>Reproductibilité des résultats (MeSH)</term>
<term>Tabac (génétique)</term>
<term>Transformation génétique (MeSH)</term>
<term>Végétaux génétiquement modifiés (MeSH)</term>
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<term>Arabidopsis</term>
<term>Fragaria</term>
<term>Plant Leaves</term>
<term>Tobacco</term>
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<term>Tabac</term>
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<term>Reproducibility of Results</term>
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<term>Végétaux génétiquement modifiés</term>
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<front><div type="abstract" xml:lang="en"><p><b>OBJECTIVE</b>
</p>
<p>The treatment of plant tissue with Agrobacterium tumefaciens is often a critical first step to both stable and transient plant transformation. In both applications bacterial suspensions are oftentimes physically introduced into plant tissues using hand-driven pressure from a needleless syringe. While effective, this approach has several drawbacks that limit reproducibility. Pressure must be provided with the syringe perfectly perpendicular to the tissue surface. The researcher must also attempt to provide even and consistent pressure, both within and between experimental replicates. These factors mean that the procedures do not always translate well between research groups or biological replicates.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>RESULTS</b>
</p>
<p>We have devised a method to introduce Agrobacterium suspensions into plant leaves with greater reproducibility. Using a decommissioned dissecting microscope as an armature, a syringe body with the bacterial suspension is mounted to the nosepiece. Gentle, even pressure is applied by rotating the focus knob. The treatment force is measured using a basic kitchen scale. The development of the Standardized Pressure Agrobacterium Infiltration Device (SPAID) provides a means to deliver consistent amounts of bacterial suspensions into plant tissues with the goal of increasing reproducibility between replicates and laboratories.</p>
</div>
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<ReferenceList><Reference><Citation>Plant J. 1998 Dec;16(6):735-43</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10069079</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell Rep. 2003 Nov;22(4):274-81</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14586552</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Methods. 2005 Dec 18;1:13</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16359558</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Methods Mol Biol. 2006;343:143-54</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16988341</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2006 Dec;48(5):818-26</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17092319</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell Rep. 2009 Mar;28(3):387-95</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19048258</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell Rep. 2009 Jun;28(6):903-13</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19308413</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2009 Jul;59(1):150-62</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19309457</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2009 Jun 05;4(6):e5812</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19503835</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Methods. 2009 Oct 31;5:15</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19878589</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Vis Exp. 2011 Sep 16;(55):null</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21947026</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Vis Exp. 2013 Jul 23;(77):null</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23913006</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell Rep. 1995 Apr;14(7):450-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24185455</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2014 Jan 08;9(1):e83556</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24416168</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Vis Exp. 2014 Apr 19;(86):null</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24796351</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Vis Exp. 2014 Dec 19;(94):null</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25549243</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Methods. 2016 Oct 19;12:41</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27777610</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Biotechnol J. 2017 May;15(5):658-668</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27862816</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biotechnol Rep (Amst). 2015 Jan 23;6:36-40</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28626695</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Methods. 2018 Aug 25;14:71</Citation>
<ArticleIdList><ArticleId IdType="pubmed">30159002</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
</record>
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