Agrobacterium tumefaciens-mediated transformation of maize embryos using a standard binary vector system.
Identifieur interne : 000855 ( Main/Corpus ); précédent : 000854; suivant : 000856Agrobacterium tumefaciens-mediated transformation of maize embryos using a standard binary vector system.
Auteurs : Bronwyn R. Frame ; Huixia Shou ; Rachel K. Chikwamba ; Zhanyuan Zhang ; Chengbin Xiang ; Tina M. Fonger ; Sue Ellen K. Pegg ; Baochun Li ; Dan S. Nettleton ; Deqing Pei ; Kan WangSource :
- Plant physiology [ 0032-0889 ] ; 2002.
English descriptors
- KwdEn :
- Agrobacterium tumefaciens (genetics), Cysteine (pharmacology), Fertility (MeSH), Gene Expression Regulation, Plant (MeSH), Genetic Complementation Test (MeSH), Genetic Vectors (genetics), Glucuronidase (genetics), Glucuronidase (metabolism), Herbicides (pharmacology), Organophosphorus Compounds (pharmacology), Plants, Genetically Modified (MeSH), Seeds (drug effects), Seeds (genetics), Seeds (growth & development), Transformation, Genetic (MeSH), Zea mays (drug effects), Zea mays (genetics), Zea mays (microbiology).
- MESH :
- chemical , genetics : Glucuronidase.
- chemical , metabolism : Glucuronidase.
- chemical , pharmacology : Cysteine, Herbicides, Organophosphorus Compounds.
- drug effects : Seeds, Zea mays.
- genetics : Agrobacterium tumefaciens, Genetic Vectors, Seeds, Zea mays.
- growth & development : Seeds.
- microbiology : Zea mays.
- Fertility, Gene Expression Regulation, Plant, Genetic Complementation Test, Plants, Genetically Modified, Transformation, Genetic.
Abstract
We have achieved routine transformation of maize (Zea mays) using an Agrobacterium tumefaciens standard binary (non-super binary) vector system. Immature zygotic embryos of the hybrid line Hi II were infected with A. tumefaciens strain EHA101 harboring a standard binary vector and cocultivated in the presence of 400 mg L-1 L-cysteine. Inclusion of L-cysteine in cocultivation medium lead to an improvement in transient beta-glucuronidase expression observed in targeted cells and a significant increase in stable transformation efficiency, but was associated with a decrease in embryo response after cocultivation. The average stable transformation efficiency (no. of bialaphos-resistant events recovered per 100 embryos infected) of the present protocol was 5.5%. Southern-blot and progeny analyses confirmed the integration, expression, and inheritance of the bar and gus transgenes in R0, R1, and R2 generations of transgenic events. To our knowledge, this represents the first report in which fertile, stable transgenic maize has been routinely produced using an A. tumefaciens standard binary vector system.
DOI: 10.1104/pp.000653
PubMed: 12011333
PubMed Central: PMC1540222
Links to Exploration step
pubmed:12011333Le document en format XML
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Chikwamba</name></author><author><name sortKey="Zhang, Zhanyuan" sort="Zhang, Zhanyuan" uniqKey="Zhang Z" first="Zhanyuan" last="Zhang">Zhanyuan Zhang</name></author><author><name sortKey="Xiang, Chengbin" sort="Xiang, Chengbin" uniqKey="Xiang C" first="Chengbin" last="Xiang">Chengbin Xiang</name></author><author><name sortKey="Fonger, Tina M" sort="Fonger, Tina M" uniqKey="Fonger T" first="Tina M" last="Fonger">Tina M. Fonger</name></author><author><name sortKey="Pegg, Sue Ellen K" sort="Pegg, Sue Ellen K" uniqKey="Pegg S" first="Sue Ellen K" last="Pegg">Sue Ellen K. Pegg</name></author><author><name sortKey="Li, Baochun" sort="Li, Baochun" uniqKey="Li B" first="Baochun" last="Li">Baochun Li</name></author><author><name sortKey="Nettleton, Dan S" sort="Nettleton, Dan S" uniqKey="Nettleton D" first="Dan S" last="Nettleton">Dan S. Nettleton</name></author><author><name sortKey="Pei, Deqing" sort="Pei, Deqing" uniqKey="Pei D" first="Deqing" last="Pei">Deqing Pei</name></author><author><name sortKey="Wang, Kan" sort="Wang, Kan" uniqKey="Wang K" first="Kan" last="Wang">Kan Wang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2002">2002</date><idno type="RBID">pubmed:12011333</idno><idno type="pmid">12011333</idno><idno type="doi">10.1104/pp.000653</idno><idno type="pmc">PMC1540222</idno><idno type="wicri:Area/Main/Corpus">000855</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000855</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Agrobacterium tumefaciens-mediated transformation of maize embryos using a standard binary vector system.</title><author><name sortKey="Frame, Bronwyn R" sort="Frame, Bronwyn R" uniqKey="Frame B" first="Bronwyn R" last="Frame">Bronwyn R. Frame</name><affiliation><nlm:affiliation>Plant Transformation Facility, Department of Agronomy, Iowa State University, Ames, Iowa 50011, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Shou, Huixia" sort="Shou, Huixia" uniqKey="Shou H" first="Huixia" last="Shou">Huixia Shou</name></author><author><name sortKey="Chikwamba, Rachel K" sort="Chikwamba, Rachel K" uniqKey="Chikwamba R" first="Rachel K" last="Chikwamba">Rachel K. Chikwamba</name></author><author><name sortKey="Zhang, Zhanyuan" sort="Zhang, Zhanyuan" uniqKey="Zhang Z" first="Zhanyuan" last="Zhang">Zhanyuan Zhang</name></author><author><name sortKey="Xiang, Chengbin" sort="Xiang, Chengbin" uniqKey="Xiang C" first="Chengbin" last="Xiang">Chengbin Xiang</name></author><author><name sortKey="Fonger, Tina M" sort="Fonger, Tina M" uniqKey="Fonger T" first="Tina M" last="Fonger">Tina M. Fonger</name></author><author><name sortKey="Pegg, Sue Ellen K" sort="Pegg, Sue Ellen K" uniqKey="Pegg S" first="Sue Ellen K" last="Pegg">Sue Ellen K. Pegg</name></author><author><name sortKey="Li, Baochun" sort="Li, Baochun" uniqKey="Li B" first="Baochun" last="Li">Baochun Li</name></author><author><name sortKey="Nettleton, Dan S" sort="Nettleton, Dan S" uniqKey="Nettleton D" first="Dan S" last="Nettleton">Dan S. Nettleton</name></author><author><name sortKey="Pei, Deqing" sort="Pei, Deqing" uniqKey="Pei D" first="Deqing" last="Pei">Deqing Pei</name></author><author><name sortKey="Wang, Kan" sort="Wang, Kan" uniqKey="Wang K" first="Kan" last="Wang">Kan Wang</name></author></analytic><series><title level="j">Plant physiology</title><idno type="ISSN">0032-0889</idno><imprint><date when="2002" type="published">2002</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium tumefaciens (genetics)</term><term>Cysteine (pharmacology)</term><term>Fertility (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Genetic Complementation Test (MeSH)</term><term>Genetic Vectors (genetics)</term><term>Glucuronidase (genetics)</term><term>Glucuronidase (metabolism)</term><term>Herbicides (pharmacology)</term><term>Organophosphorus Compounds (pharmacology)</term><term>Plants, Genetically Modified (MeSH)</term><term>Seeds (drug effects)</term><term>Seeds (genetics)</term><term>Seeds (growth & development)</term><term>Transformation, Genetic (MeSH)</term><term>Zea mays (drug effects)</term><term>Zea mays (genetics)</term><term>Zea mays (microbiology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Glucuronidase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Glucuronidase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Cysteine</term><term>Herbicides</term><term>Organophosphorus Compounds</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Seeds</term><term>Zea mays</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agrobacterium tumefaciens</term><term>Genetic Vectors</term><term>Seeds</term><term>Zea mays</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Seeds</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Zea mays</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Fertility</term><term>Gene Expression Regulation, Plant</term><term>Genetic Complementation Test</term><term>Plants, Genetically Modified</term><term>Transformation, Genetic</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have achieved routine transformation of maize (Zea mays) using an Agrobacterium tumefaciens standard binary (non-super binary) vector system. Immature zygotic embryos of the hybrid line Hi II were infected with A. tumefaciens strain EHA101 harboring a standard binary vector and cocultivated in the presence of 400 mg L-1 L-cysteine. Inclusion of L-cysteine in cocultivation medium lead to an improvement in transient beta-glucuronidase expression observed in targeted cells and a significant increase in stable transformation efficiency, but was associated with a decrease in embryo response after cocultivation. The average stable transformation efficiency (no. of bialaphos-resistant events recovered per 100 embryos infected) of the present protocol was 5.5%. Southern-blot and progeny analyses confirmed the integration, expression, and inheritance of the bar and gus transgenes in R0, R1, and R2 generations of transgenic events. To our knowledge, this represents the first report in which fertile, stable transgenic maize has been routinely produced using an A. tumefaciens standard binary vector system.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">12011333</PMID><DateCompleted><Year>2002</Year><Month>08</Month><Day>09</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0032-0889</ISSN><JournalIssue CitedMedium="Print"><Volume>129</Volume><Issue>1</Issue><PubDate><Year>2002</Year><Month>May</Month></PubDate></JournalIssue><Title>Plant physiology</Title><ISOAbbreviation>Plant Physiol</ISOAbbreviation></Journal><ArticleTitle>Agrobacterium tumefaciens-mediated transformation of maize embryos using a standard binary vector system.</ArticleTitle><Pagination><MedlinePgn>13-22</MedlinePgn></Pagination><Abstract><AbstractText>We have achieved routine transformation of maize (Zea mays) using an Agrobacterium tumefaciens standard binary (non-super binary) vector system. Immature zygotic embryos of the hybrid line Hi II were infected with A. tumefaciens strain EHA101 harboring a standard binary vector and cocultivated in the presence of 400 mg L-1 L-cysteine. Inclusion of L-cysteine in cocultivation medium lead to an improvement in transient beta-glucuronidase expression observed in targeted cells and a significant increase in stable transformation efficiency, but was associated with a decrease in embryo response after cocultivation. The average stable transformation efficiency (no. of bialaphos-resistant events recovered per 100 embryos infected) of the present protocol was 5.5%. Southern-blot and progeny analyses confirmed the integration, expression, and inheritance of the bar and gus transgenes in R0, R1, and R2 generations of transgenic events. To our knowledge, this represents the first report in which fertile, stable transgenic maize has been routinely produced using an A. tumefaciens standard binary vector system.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Frame</LastName><ForeName>Bronwyn R</ForeName><Initials>BR</Initials><AffiliationInfo><Affiliation>Plant Transformation Facility, Department of Agronomy, Iowa State University, Ames, Iowa 50011, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shou</LastName><ForeName>Huixia</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Chikwamba</LastName><ForeName>Rachel K</ForeName><Initials>RK</Initials></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Zhanyuan</ForeName><Initials>Z</Initials></Author><Author ValidYN="Y"><LastName>Xiang</LastName><ForeName>Chengbin</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Fonger</LastName><ForeName>Tina M</ForeName><Initials>TM</Initials></Author><Author ValidYN="Y"><LastName>Pegg</LastName><ForeName>Sue Ellen K</ForeName><Initials>SE</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Baochun</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Nettleton</LastName><ForeName>Dan S</ForeName><Initials>DS</Initials></Author><Author ValidYN="Y"><LastName>Pei</LastName><ForeName>Deqing</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Kan</ForeName><Initials>K</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plant Physiol</MedlineTA><NlmUniqueID>0401224</NlmUniqueID><ISSNLinking>0032-0889</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D006540">Herbicides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009943">Organophosphorus Compounds</NameOfSubstance></Chemical><Chemical><RegistryNumber>35597-43-4</RegistryNumber><NameOfSubstance UI="C003122">bialaphos</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.31</RegistryNumber><NameOfSubstance UI="D005966">Glucuronidase</NameOfSubstance></Chemical><Chemical><RegistryNumber>K848JZ4886</RegistryNumber><NameOfSubstance UI="D003545">Cysteine</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D016960" MajorTopicYN="N">Agrobacterium tumefaciens</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003545" MajorTopicYN="N">Cysteine</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005298" MajorTopicYN="N">Fertility</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005816" MajorTopicYN="N">Genetic Complementation Test</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005822" MajorTopicYN="N">Genetic Vectors</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005966" MajorTopicYN="N">Glucuronidase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006540" MajorTopicYN="N">Herbicides</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009943" MajorTopicYN="N">Organophosphorus Compounds</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012639" MajorTopicYN="N">Seeds</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003313" MajorTopicYN="N">Zea mays</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2002</Year><Month>5</Month><Day>16</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2002</Year><Month>8</Month><Day>10</Day><Hour>10</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2002</Year><Month>5</Month><Day>16</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">12011333</ArticleId><ArticleId IdType="doi">10.1104/pp.000653</ArticleId><ArticleId IdType="pmc">PMC1540222</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour 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