Variability in the level of RNA silencing suppression caused by triple gene block protein 1 (TGBp1) from various potexviruses during infection.
Identifieur interne : 000643 ( Main/Corpus ); précédent : 000642; suivant : 000644Variability in the level of RNA silencing suppression caused by triple gene block protein 1 (TGBp1) from various potexviruses during infection.
Auteurs : Hiroko Senshu ; Johji Ozeki ; Ken Komatsu ; Masayoshi Hashimoto ; Kouji Hatada ; Michiko Aoyama ; Satoshi Kagiwada ; Yasuyuki Yamaji ; Shigetou NambaSource :
- The Journal of general virology [ 0022-1317 ] ; 2009.
English descriptors
- KwdEn :
- Gene Expression Regulation (MeSH), Green Fluorescent Proteins (genetics), Green Fluorescent Proteins (metabolism), MicroRNAs (genetics), MicroRNAs (metabolism), Plant Diseases (virology), Plant Leaves (virology), Potexvirus (classification), Potexvirus (genetics), Potexvirus (pathogenicity), RNA Interference (drug effects), RNA, Small Interfering (genetics), RNA, Small Interfering (metabolism), RNA, Viral (genetics), RNA, Viral (metabolism), Rhizobium (virology), Tobacco (virology), Transgenes (MeSH), Viral Proteins (genetics), Viral Proteins (metabolism), Viral Proteins (pharmacology).
- MESH :
- chemical , genetics : Green Fluorescent Proteins, MicroRNAs, RNA, Small Interfering, RNA, Viral, Viral Proteins.
- chemical , metabolism : Green Fluorescent Proteins, MicroRNAs, RNA, Small Interfering, RNA, Viral, Viral Proteins.
- classification : Potexvirus.
- drug effects : RNA Interference.
- genetics : Potexvirus.
- pathogenicity : Potexvirus.
- chemical , pharmacology : Viral Proteins.
- virology : Plant Diseases, Plant Leaves, Rhizobium, Tobacco.
- Gene Expression Regulation, Transgenes.
Abstract
RNA silencing is an important defence mechanism against virus infection, and many plant viruses encode RNA silencing suppressors as a counter defence. In this study, we analysed the RNA silencing suppression ability of multiple virus species of the genus Potexvirus. Nicotiana benthamiana plants exhibiting RNA silencing of a green fluorescent protein (GFP) transgene showed reversal of GFP fluorescence when systemically infected with potexviruses. However, the degree of GFP fluorescence varied among potexviruses. Agrobacterium-mediated transient expression assay in N. benthamiana leaves demonstrated that the triple gene block protein 1 (TGBp1) encoded by these potexviruses has drastically different levels of silencing suppressor activity, and these differences were directly related to variations in the silencing suppression ability during virus infection. These results suggest that suppressor activities differ even among homologous proteins encoded by viruses of the same genus, and that TGBp1 contributes to the variation in the level of RNA silencing suppression by potexviruses. Moreover, we investigated the effect of TGBp1 encoded by Plantago asiatica mosaic virus (PlAMV), which exhibited a strong suppressor activity, on the accumulation of microRNA, virus genomic RNA and virus-derived small interfering RNAs.
DOI: 10.1099/vir.0.008243-0
PubMed: 19264652
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pubmed:19264652Le document en format XML
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Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Komatsu, Ken" sort="Komatsu, Ken" uniqKey="Komatsu K" first="Ken" last="Komatsu">Ken Komatsu</name><affiliation><nlm:affiliation>Laboratory of Plant Pathology, Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Hashimoto, Masayoshi" sort="Hashimoto, Masayoshi" uniqKey="Hashimoto M" first="Masayoshi" last="Hashimoto">Masayoshi Hashimoto</name><affiliation><nlm:affiliation>Laboratory of Plant Pathology, Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Hatada, Kouji" sort="Hatada, Kouji" uniqKey="Hatada K" first="Kouji" last="Hatada">Kouji 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Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Yamaji, Yasuyuki" sort="Yamaji, Yasuyuki" uniqKey="Yamaji Y" first="Yasuyuki" last="Yamaji">Yasuyuki Yamaji</name><affiliation><nlm:affiliation>Laboratory of Plant Pathology, Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Namba, Shigetou" sort="Namba, Shigetou" uniqKey="Namba S" first="Shigetou" last="Namba">Shigetou Namba</name><affiliation><nlm:affiliation>Laboratory of Plant Pathology, Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno 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last="Ozeki">Johji Ozeki</name><affiliation><nlm:affiliation>Laboratory of Plant Pathology, Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Komatsu, Ken" sort="Komatsu, Ken" uniqKey="Komatsu K" first="Ken" last="Komatsu">Ken Komatsu</name><affiliation><nlm:affiliation>Laboratory of Plant Pathology, Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Hashimoto, Masayoshi" sort="Hashimoto, Masayoshi" uniqKey="Hashimoto M" first="Masayoshi" last="Hashimoto">Masayoshi Hashimoto</name><affiliation><nlm:affiliation>Laboratory of Plant Pathology, Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Hatada, Kouji" sort="Hatada, Kouji" uniqKey="Hatada K" first="Kouji" last="Hatada">Kouji Hatada</name><affiliation><nlm:affiliation>Laboratory of Plant Pathology, Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Aoyama, Michiko" sort="Aoyama, Michiko" uniqKey="Aoyama M" first="Michiko" last="Aoyama">Michiko Aoyama</name><affiliation><nlm:affiliation>Laboratory of Plant Pathology, Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Kagiwada, Satoshi" sort="Kagiwada, Satoshi" uniqKey="Kagiwada S" first="Satoshi" last="Kagiwada">Satoshi Kagiwada</name><affiliation><nlm:affiliation>Department of Clinical Plant Science, Faculty of Bioscience and Applied Chemistry, Hosei University, Tokyo, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Yamaji, Yasuyuki" sort="Yamaji, Yasuyuki" uniqKey="Yamaji Y" first="Yasuyuki" last="Yamaji">Yasuyuki Yamaji</name><affiliation><nlm:affiliation>Laboratory of Plant Pathology, Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Namba, Shigetou" sort="Namba, Shigetou" uniqKey="Namba S" first="Shigetou" last="Namba">Shigetou Namba</name><affiliation><nlm:affiliation>Laboratory of Plant Pathology, Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.</nlm:affiliation></affiliation></author></analytic><series><title level="j">The Journal of general virology</title><idno type="ISSN">0022-1317</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Gene Expression Regulation (MeSH)</term><term>Green Fluorescent Proteins (genetics)</term><term>Green Fluorescent Proteins (metabolism)</term><term>MicroRNAs (genetics)</term><term>MicroRNAs (metabolism)</term><term>Plant Diseases (virology)</term><term>Plant Leaves (virology)</term><term>Potexvirus (classification)</term><term>Potexvirus (genetics)</term><term>Potexvirus (pathogenicity)</term><term>RNA Interference (drug effects)</term><term>RNA, Small Interfering (genetics)</term><term>RNA, Small Interfering (metabolism)</term><term>RNA, Viral (genetics)</term><term>RNA, Viral (metabolism)</term><term>Rhizobium (virology)</term><term>Tobacco (virology)</term><term>Transgenes (MeSH)</term><term>Viral Proteins (genetics)</term><term>Viral Proteins (metabolism)</term><term>Viral Proteins (pharmacology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Green Fluorescent Proteins</term><term>MicroRNAs</term><term>RNA, Small Interfering</term><term>RNA, Viral</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Green Fluorescent Proteins</term><term>MicroRNAs</term><term>RNA, Small Interfering</term><term>RNA, Viral</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Potexvirus</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>RNA Interference</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Potexvirus</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Potexvirus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Plant Diseases</term><term>Plant Leaves</term><term>Rhizobium</term><term>Tobacco</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation</term><term>Transgenes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">RNA silencing is an important defence mechanism against virus infection, and many plant viruses encode RNA silencing suppressors as a counter defence. In this study, we analysed the RNA silencing suppression ability of multiple virus species of the genus Potexvirus. Nicotiana benthamiana plants exhibiting RNA silencing of a green fluorescent protein (GFP) transgene showed reversal of GFP fluorescence when systemically infected with potexviruses. However, the degree of GFP fluorescence varied among potexviruses. Agrobacterium-mediated transient expression assay in N. benthamiana leaves demonstrated that the triple gene block protein 1 (TGBp1) encoded by these potexviruses has drastically different levels of silencing suppressor activity, and these differences were directly related to variations in the silencing suppression ability during virus infection. These results suggest that suppressor activities differ even among homologous proteins encoded by viruses of the same genus, and that TGBp1 contributes to the variation in the level of RNA silencing suppression by potexviruses. Moreover, we investigated the effect of TGBp1 encoded by Plantago asiatica mosaic virus (PlAMV), which exhibited a strong suppressor activity, on the accumulation of microRNA, virus genomic RNA and virus-derived small interfering RNAs.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19264652</PMID><DateCompleted><Year>2009</Year><Month>05</Month><Day>11</Day></DateCompleted><DateRevised><Year>2020</Year><Month>03</Month><Day>02</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0022-1317</ISSN><JournalIssue CitedMedium="Print"><Volume>90</Volume><Issue>Pt 4</Issue><PubDate><Year>2009</Year><Month>Apr</Month></PubDate></JournalIssue><Title>The Journal of general virology</Title><ISOAbbreviation>J Gen Virol</ISOAbbreviation></Journal><ArticleTitle>Variability in the level of RNA silencing suppression caused by triple gene block protein 1 (TGBp1) from various potexviruses during infection.</ArticleTitle><Pagination><MedlinePgn>1014-1024</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1099/vir.0.008243-0</ELocationID><Abstract><AbstractText>RNA silencing is an important defence mechanism against virus infection, and many plant viruses encode RNA silencing suppressors as a counter defence. In this study, we analysed the RNA silencing suppression ability of multiple virus species of the genus Potexvirus. Nicotiana benthamiana plants exhibiting RNA silencing of a green fluorescent protein (GFP) transgene showed reversal of GFP fluorescence when systemically infected with potexviruses. However, the degree of GFP fluorescence varied among potexviruses. Agrobacterium-mediated transient expression assay in N. benthamiana leaves demonstrated that the triple gene block protein 1 (TGBp1) encoded by these potexviruses has drastically different levels of silencing suppressor activity, and these differences were directly related to variations in the silencing suppression ability during virus infection. These results suggest that suppressor activities differ even among homologous proteins encoded by viruses of the same genus, and that TGBp1 contributes to the variation in the level of RNA silencing suppression by potexviruses. Moreover, we investigated the effect of TGBp1 encoded by Plantago asiatica mosaic virus (PlAMV), which exhibited a strong suppressor activity, on the accumulation of microRNA, virus genomic RNA and virus-derived small interfering RNAs.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Senshu</LastName><ForeName>Hiroko</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Laboratory of Plant Pathology, Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ozeki</LastName><ForeName>Johji</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Laboratory of Plant Pathology, Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Komatsu</LastName><ForeName>Ken</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Laboratory of Plant Pathology, Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hashimoto</LastName><ForeName>Masayoshi</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Laboratory of Plant Pathology, Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hatada</LastName><ForeName>Kouji</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Laboratory of Plant Pathology, Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Aoyama</LastName><ForeName>Michiko</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Laboratory of Plant Pathology, Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kagiwada</LastName><ForeName>Satoshi</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Clinical Plant Science, Faculty of Bioscience and Applied Chemistry, Hosei University, Tokyo, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yamaji</LastName><ForeName>Yasuyuki</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Laboratory of Plant Pathology, Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Namba</LastName><ForeName>Shigetou</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Laboratory of Plant Pathology, Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2009</Year><Month>03</Month><Day>04</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Gen Virol</MedlineTA><NlmUniqueID>0077340</NlmUniqueID><ISSNLinking>0022-1317</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D035683">MicroRNAs</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D034741">RNA, Small Interfering</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014764">Viral Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>147336-22-9</RegistryNumber><NameOfSubstance UI="D049452">Green Fluorescent Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D005786" MajorTopicYN="N">Gene Expression Regulation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D049452" MajorTopicYN="N">Green Fluorescent 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MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000472" MajorTopicYN="Y">pathogenicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D034622" MajorTopicYN="N">RNA Interference</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D034741" MajorTopicYN="N">RNA, Small Interfering</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012231" MajorTopicYN="N">Rhizobium</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName 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