Ectopic expression of MgSM1, a Cerato-platanin family protein from Magnaporthe grisea, confers broad-spectrum disease resistance in Arabidopsis.
Identifieur interne : 000626 ( Main/Corpus ); précédent : 000625; suivant : 000627Ectopic expression of MgSM1, a Cerato-platanin family protein from Magnaporthe grisea, confers broad-spectrum disease resistance in Arabidopsis.
Auteurs : Yayun Yang ; Huijuan Zhang ; Guojun Li ; Wei Li ; Xiao'E Wang ; Fengming SongSource :
- Plant biotechnology journal [ 1467-7652 ] ; 2009.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Arabidopsis (genetics), Arabidopsis (immunology), Arabidopsis (metabolism), Fungal Proteins (genetics), Fungal Proteins (metabolism), Gene Expression Regulation, Plant (MeSH), Genes, Fungal (MeSH), Immunity, Innate (MeSH), Magnaporthe (genetics), Molecular Sequence Data (MeSH), Plant Diseases (genetics), Plant Diseases (immunology), Plant Leaves (genetics), Plant Leaves (immunology), Plant Leaves (metabolism), Plants, Genetically Modified (genetics), Plants, Genetically Modified (immunology), Plants, Genetically Modified (metabolism), Reactive Oxygen Species (metabolism), Sequence Alignment (MeSH), Transformation, Genetic (MeSH).
- MESH :
- chemical , genetics : Fungal Proteins.
- genetics : Arabidopsis, Magnaporthe, Plant Diseases, Plant Leaves, Plants, Genetically Modified.
- immunology : Arabidopsis, Plant Diseases, Plant Leaves, Plants, Genetically Modified.
- metabolism : Arabidopsis, Fungal Proteins, Plant Leaves, Plants, Genetically Modified, Reactive Oxygen Species.
- Amino Acid Sequence, Gene Expression Regulation, Plant, Genes, Fungal, Immunity, Innate, Molecular Sequence Data, Sequence Alignment, Transformation, Genetic.
Abstract
Proteins belonging to the newly identified Cerato-platanin (CP) family have been shown to have elicitor activity in inducing disease resistance responses in various plants. In this study, we characterized a gene, MgSM1, from Magnaporthe grisea, encoding a putative small protein belonging to the CP family. MgSM1 was constitutively expressed not only in different fungal growth stages but also during its infection process in rice plants. Agrobacterium-mediated transient expression of MgSM1 in Arabidopsis resulted in hypersensitive response in the infiltrated local leaves and enhanced disease resistance against Botrytis cinerea and Pseudomonas syringae pv. tomato (Pst) DC3000 in upper leaves of plants, accompanyed by up-regulated expression of defense genes (PR-1, PR-5 and PDF1.2). Transgenic Arabidopsis plants expressing MgSM1 under control of a dexamethasone (DEX)-inducible promoter were generated. Expression of MgSM1 in transgenic plants was induced by exogenous application of DEX. MgSM1-expressing plants showed normal growth with application of <10 microm DEX. After DEX induction, the MgSM1-expressing plants showed enhanced disease resistance against B. cinerea, Alternaria brassicicola and Psto DC3000 as well as up-regulated expression of some of defense genes. Moreover, accumulation of reactive oxygen species was observed in MgSM1-expressing plants. These results collectively suggest that ectopic expression of MgSM1 in transgenic plants confers broad-spectrum resistance against different types of pathogens. Our study also provides a novel strategy to generate environment-friendly crops with enhanced broad-spectrum resistance through ectopic expression of microbe-derived disease resistance-inducing proteins.
DOI: 10.1111/j.1467-7652.2009.00442.x
PubMed: 19754836
Links to Exploration step
pubmed:19754836Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Ectopic expression of MgSM1, a Cerato-platanin family protein from Magnaporthe grisea, confers broad-spectrum disease resistance in Arabidopsis.</title><author><name sortKey="Yang, Yayun" sort="Yang, Yayun" uniqKey="Yang Y" first="Yayun" last="Yang">Yayun Yang</name><affiliation><nlm:affiliation>State Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhang, Huijuan" sort="Zhang, Huijuan" uniqKey="Zhang H" first="Huijuan" last="Zhang">Huijuan Zhang</name></author><author><name sortKey="Li, Guojun" sort="Li, Guojun" uniqKey="Li G" first="Guojun" last="Li">Guojun Li</name></author><author><name sortKey="Li, Wei" sort="Li, Wei" uniqKey="Li W" first="Wei" last="Li">Wei Li</name></author><author><name sortKey="Wang, Xiao E" sort="Wang, Xiao E" uniqKey="Wang X" first="Xiao'E" last="Wang">Xiao'E Wang</name></author><author><name sortKey="Song, Fengming" sort="Song, Fengming" uniqKey="Song F" first="Fengming" last="Song">Fengming Song</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19754836</idno><idno type="pmid">19754836</idno><idno type="doi">10.1111/j.1467-7652.2009.00442.x</idno><idno type="wicri:Area/Main/Corpus">000626</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000626</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Ectopic expression of MgSM1, a Cerato-platanin family protein from Magnaporthe grisea, confers broad-spectrum disease resistance in Arabidopsis.</title><author><name sortKey="Yang, Yayun" sort="Yang, Yayun" uniqKey="Yang Y" first="Yayun" last="Yang">Yayun Yang</name><affiliation><nlm:affiliation>State Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhang, Huijuan" sort="Zhang, Huijuan" uniqKey="Zhang H" first="Huijuan" last="Zhang">Huijuan Zhang</name></author><author><name sortKey="Li, Guojun" sort="Li, Guojun" uniqKey="Li G" first="Guojun" last="Li">Guojun Li</name></author><author><name sortKey="Li, Wei" sort="Li, Wei" uniqKey="Li W" first="Wei" last="Li">Wei Li</name></author><author><name sortKey="Wang, Xiao E" sort="Wang, Xiao E" uniqKey="Wang X" first="Xiao'E" last="Wang">Xiao'E Wang</name></author><author><name sortKey="Song, Fengming" sort="Song, Fengming" uniqKey="Song F" first="Fengming" last="Song">Fengming Song</name></author></analytic><series><title level="j">Plant biotechnology journal</title><idno type="eISSN">1467-7652</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Arabidopsis (genetics)</term><term>Arabidopsis (immunology)</term><term>Arabidopsis (metabolism)</term><term>Fungal Proteins (genetics)</term><term>Fungal Proteins (metabolism)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Genes, Fungal (MeSH)</term><term>Immunity, Innate (MeSH)</term><term>Magnaporthe (genetics)</term><term>Molecular Sequence Data (MeSH)</term><term>Plant Diseases (genetics)</term><term>Plant Diseases (immunology)</term><term>Plant Leaves (genetics)</term><term>Plant Leaves (immunology)</term><term>Plant Leaves (metabolism)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (immunology)</term><term>Plants, Genetically Modified (metabolism)</term><term>Reactive Oxygen Species (metabolism)</term><term>Sequence Alignment (MeSH)</term><term>Transformation, Genetic (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Fungal Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Arabidopsis</term><term>Magnaporthe</term><term>Plant Diseases</term><term>Plant Leaves</term><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Arabidopsis</term><term>Plant Diseases</term><term>Plant Leaves</term><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Arabidopsis</term><term>Fungal Proteins</term><term>Plant Leaves</term><term>Plants, Genetically Modified</term><term>Reactive Oxygen Species</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Gene Expression Regulation, Plant</term><term>Genes, Fungal</term><term>Immunity, Innate</term><term>Molecular Sequence Data</term><term>Sequence Alignment</term><term>Transformation, Genetic</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Proteins belonging to the newly identified Cerato-platanin (CP) family have been shown to have elicitor activity in inducing disease resistance responses in various plants. In this study, we characterized a gene, MgSM1, from Magnaporthe grisea, encoding a putative small protein belonging to the CP family. MgSM1 was constitutively expressed not only in different fungal growth stages but also during its infection process in rice plants. Agrobacterium-mediated transient expression of MgSM1 in Arabidopsis resulted in hypersensitive response in the infiltrated local leaves and enhanced disease resistance against Botrytis cinerea and Pseudomonas syringae pv. tomato (Pst) DC3000 in upper leaves of plants, accompanyed by up-regulated expression of defense genes (PR-1, PR-5 and PDF1.2). Transgenic Arabidopsis plants expressing MgSM1 under control of a dexamethasone (DEX)-inducible promoter were generated. Expression of MgSM1 in transgenic plants was induced by exogenous application of DEX. MgSM1-expressing plants showed normal growth with application of <10 microm DEX. After DEX induction, the MgSM1-expressing plants showed enhanced disease resistance against B. cinerea, Alternaria brassicicola and Psto DC3000 as well as up-regulated expression of some of defense genes. Moreover, accumulation of reactive oxygen species was observed in MgSM1-expressing plants. These results collectively suggest that ectopic expression of MgSM1 in transgenic plants confers broad-spectrum resistance against different types of pathogens. Our study also provides a novel strategy to generate environment-friendly crops with enhanced broad-spectrum resistance through ectopic expression of microbe-derived disease resistance-inducing proteins.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19754836</PMID><DateCompleted><Year>2009</Year><Month>10</Month><Day>21</Day></DateCompleted><DateRevised><Year>2009</Year><Month>09</Month><Day>16</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1467-7652</ISSN><JournalIssue CitedMedium="Internet"><Volume>7</Volume><Issue>8</Issue><PubDate><Year>2009</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Plant biotechnology journal</Title><ISOAbbreviation>Plant Biotechnol J</ISOAbbreviation></Journal><ArticleTitle>Ectopic expression of MgSM1, a Cerato-platanin family protein from Magnaporthe grisea, confers broad-spectrum disease resistance in Arabidopsis.</ArticleTitle><Pagination><MedlinePgn>763-77</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/j.1467-7652.2009.00442.x</ELocationID><Abstract><AbstractText>Proteins belonging to the newly identified Cerato-platanin (CP) family have been shown to have elicitor activity in inducing disease resistance responses in various plants. In this study, we characterized a gene, MgSM1, from Magnaporthe grisea, encoding a putative small protein belonging to the CP family. MgSM1 was constitutively expressed not only in different fungal growth stages but also during its infection process in rice plants. Agrobacterium-mediated transient expression of MgSM1 in Arabidopsis resulted in hypersensitive response in the infiltrated local leaves and enhanced disease resistance against Botrytis cinerea and Pseudomonas syringae pv. tomato (Pst) DC3000 in upper leaves of plants, accompanyed by up-regulated expression of defense genes (PR-1, PR-5 and PDF1.2). Transgenic Arabidopsis plants expressing MgSM1 under control of a dexamethasone (DEX)-inducible promoter were generated. Expression of MgSM1 in transgenic plants was induced by exogenous application of DEX. MgSM1-expressing plants showed normal growth with application of <10 microm DEX. After DEX induction, the MgSM1-expressing plants showed enhanced disease resistance against B. cinerea, Alternaria brassicicola and Psto DC3000 as well as up-regulated expression of some of defense genes. Moreover, accumulation of reactive oxygen species was observed in MgSM1-expressing plants. These results collectively suggest that ectopic expression of MgSM1 in transgenic plants confers broad-spectrum resistance against different types of pathogens. Our study also provides a novel strategy to generate environment-friendly crops with enhanced broad-spectrum resistance through ectopic expression of microbe-derived disease resistance-inducing proteins.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Yayun</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>State Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Huijuan</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Guojun</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Wei</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Xiao'e</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Song</LastName><ForeName>Fengming</ForeName><Initials>F</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Plant Biotechnol J</MedlineTA><NlmUniqueID>101201889</NlmUniqueID><ISSNLinking>1467-7644</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017382">Reactive Oxygen Species</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017360" MajorTopicYN="N">Arabidopsis</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005656" MajorTopicYN="N">Fungal Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005800" MajorTopicYN="N">Genes, Fungal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007113" MajorTopicYN="Y">Immunity, Innate</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020082" MajorTopicYN="N">Magnaporthe</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017382" MajorTopicYN="N">Reactive Oxygen Species</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016415" MajorTopicYN="N">Sequence Alignment</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>9</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>9</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>10</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19754836</ArticleId><ArticleId IdType="pii">PBI442</ArticleId><ArticleId IdType="doi">10.1111/j.1467-7652.2009.00442.x</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/AgrobacTransV1/Data/Main/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000626 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd -nk 000626 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= AgrobacTransV1
|flux= Main
|étape= Corpus
|type= RBID
|clé= pubmed:19754836
|texte= Ectopic expression of MgSM1, a Cerato-platanin family protein from Magnaporthe grisea, confers broad-spectrum disease resistance in Arabidopsis.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Corpus/RBID.i -Sk "pubmed:19754836" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a AgrobacTransV1
| This area was generated with Dilib version V0.6.38. |  |