Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression.
Identifieur interne : 000382 ( Main/Corpus ); précédent : 000381; suivant : 000383Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression.
Auteurs : Y R Sindarovska ; I S Golovach ; V B Belokurova ; I M Gerasymenko ; Y V Sheludko ; N V KuchukSource :
- TSitologiia i genetika [ 0564-3783 ]
English descriptors
- KwdEn :
- Agrobacterium tumefaciens (genetics), Coculture Techniques (MeSH), Gene Expression Regulation, Plant (MeSH), Genes, Reporter (MeSH), Genetic Engineering (methods), Genetic Vectors (MeSH), Glucuronidase (genetics), Plant Cells (enzymology), Plants, Genetically Modified (enzymology), Plants, Genetically Modified (genetics), Species Specificity (MeSH), Transformation, Genetic (MeSH).
- MESH :
- chemical , genetics : Glucuronidase.
- enzymology : Plant Cells, Plants, Genetically Modified.
- genetics : Agrobacterium tumefaciens, Plants, Genetically Modified.
- methods : Genetic Engineering.
- Coculture Techniques, Gene Expression Regulation, Plant, Genes, Reporter, Genetic Vectors, Species Specificity, Transformation, Genetic.
Abstract
Agrobacterium-mediated transient expression is an approach for short-time expression of heterologous genes in plant systems. During the last decade transient expression was regarded as a potent protocol for high scale production of foreign proteins in plants including pharmaceutically valuable proteins. In vitro grown plant cell cultures represent a suitable system for accumulation of heterologous proteins under controlled conditions. Since host characteristics may strongly influence transient expression efficiency, we performed screening of undifferentiated cell cultures for transient expression ability using GUS as a reporter. Analysis of 248 plant species belonging to 49 families from the National Germplasm Bank of the World Flora of the Institute of Cell Biology and Genetic Engineering (Kyiv, Ukraine) allowed for selection of about 50 plant species exhibiting detectable beta-glucuronidase activity.
PubMed: 25181853
Links to Exploration step
pubmed:25181853Le document en format XML
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During the last decade transient expression was regarded as a potent protocol for high scale production of foreign proteins in plants including pharmaceutically valuable proteins. In vitro grown plant cell cultures represent a suitable system for accumulation of heterologous proteins under controlled conditions. Since host characteristics may strongly influence transient expression efficiency, we performed screening of undifferentiated cell cultures for transient expression ability using GUS as a reporter. Analysis of 248 plant species belonging to 49 families from the National Germplasm Bank of the World Flora of the Institute of Cell Biology and Genetic Engineering (Kyiv, Ukraine) allowed for selection of about 50 plant species exhibiting detectable beta-glucuronidase activity.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25181853</PMID><DateCompleted><Year>2014</Year><Month>09</Month><Day>16</Day></DateCompleted><DateRevised><Year>2014</Year><Month>09</Month><Day>03</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0564-3783</ISSN><JournalIssue CitedMedium="Print"><Volume>48</Volume><Issue>4</Issue><PubDate><MedlineDate>2014 Jul-Aug</MedlineDate></PubDate></JournalIssue><Title>TSitologiia i genetika</Title><ISOAbbreviation>Tsitol Genet</ISOAbbreviation></Journal><ArticleTitle>Screening of plant cell culture collection for efficient host species for Agrobacterium-mediated transient expression.</ArticleTitle><Pagination><MedlinePgn>9-18</MedlinePgn></Pagination><Abstract><AbstractText>Agrobacterium-mediated transient expression is an approach for short-time expression of heterologous genes in plant systems. During the last decade transient expression was regarded as a potent protocol for high scale production of foreign proteins in plants including pharmaceutically valuable proteins. In vitro grown plant cell cultures represent a suitable system for accumulation of heterologous proteins under controlled conditions. Since host characteristics may strongly influence transient expression efficiency, we performed screening of undifferentiated cell cultures for transient expression ability using GUS as a reporter. Analysis of 248 plant species belonging to 49 families from the National Germplasm Bank of the World Flora of the Institute of Cell Biology and Genetic Engineering (Kyiv, Ukraine) allowed for selection of about 50 plant species exhibiting detectable beta-glucuronidase activity.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Sindarovska</LastName><ForeName>Y R</ForeName><Initials>YR</Initials></Author><Author ValidYN="Y"><LastName>Golovach</LastName><ForeName>I S</ForeName><Initials>IS</Initials></Author><Author ValidYN="Y"><LastName>Belokurova</LastName><ForeName>V B</ForeName><Initials>VB</Initials></Author><Author ValidYN="Y"><LastName>Gerasymenko</LastName><ForeName>I M</ForeName><Initials>IM</Initials></Author><Author ValidYN="Y"><LastName>Sheludko</LastName><ForeName>Y V</ForeName><Initials>YV</Initials></Author><Author ValidYN="Y"><LastName>Kuchuk</LastName><ForeName>N V</ForeName><Initials>NV</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Ukraine</Country><MedlineTA>Tsitol Genet</MedlineTA><NlmUniqueID>0101671</NlmUniqueID><ISSNLinking>0564-3783</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>EC 3.2.1.31</RegistryNumber><NameOfSubstance UI="D005966">Glucuronidase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D016960" MajorTopicYN="N">Agrobacterium tumefaciens</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018920" MajorTopicYN="N">Coculture Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017930" MajorTopicYN="N">Genes, Reporter</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005818" MajorTopicYN="N">Genetic Engineering</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005822" MajorTopicYN="N">Genetic Vectors</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005966" MajorTopicYN="N">Glucuronidase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D059828" MajorTopicYN="N">Plant Cells</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013045" MajorTopicYN="N">Species Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>9</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>9</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>9</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25181853</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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