Agrobacterium-mediated transient MaFT expression in mulberry (Morus alba L.) leaves.
Identifieur interne : 000337 ( Main/Corpus ); précédent : 000336; suivant : 000338Agrobacterium-mediated transient MaFT expression in mulberry (Morus alba L.) leaves.
Auteurs : Su-Li Wu ; Xiao-Bing Yang ; Li-Qun Liu ; Tao Jiang ; Hai Wu ; Chao Su ; Yong-Hua Qian ; Feng JiaoSource :
- Bioscience, biotechnology, and biochemistry [ 1347-6947 ] ; 2015.
English descriptors
- KwdEn :
- Agrobacterium tumefaciens (genetics), Arabidopsis (genetics), Arabidopsis (growth & development), Arabidopsis Proteins (genetics), Fruit (genetics), Gene Expression Regulation, Plant (MeSH), Morus (genetics), Morus (growth & development), Plant Leaves (genetics), Plant Leaves (growth & development), Plants, Genetically Modified (genetics), Plants, Genetically Modified (growth & development), Plasmids (MeSH), Seedlings (genetics).
- MESH :
- chemical , genetics : Arabidopsis Proteins.
- genetics : Agrobacterium tumefaciens, Arabidopsis, Fruit, Morus, Plant Leaves, Plants, Genetically Modified, Seedlings.
- growth & development : Arabidopsis, Morus, Plant Leaves, Plants, Genetically Modified.
- Gene Expression Regulation, Plant, Plasmids.
Abstract
To optimize Agrobacterium-mediated transient transformation assay in mulberry (Morus alba L.), various infiltration methods, Agrobacterium tumefaciens (A. tumefaciens) strains, and bacterial concentrations were tested in mulberry seedlings. Compared with LBA4404, GV3101 harboring pBE2133 plasmids presented stronger GUS signals at 3 days post infiltration using syringe. Recombinant plasmids pBE2133:GFP and pBE2133:GFP:MaFT were successfully constructed. Transient expression of MaFT:GFP protein was found in leaves, petiole (cross section), and shoot apical meristem (SAM) of mulberry according to the GFP signal. Moreover, MaFT:GFP mRNA was also detected in leaves and SAM via RT-PCR and qRT-PCR. An efficient transient transformation system could be achieved in mulberry seedlings by syringe using A. tumefaciens GV3101 at the OD600 of 0.5. The movement of MaFT expression from leaves to SAM might trigger the precocious flowering of mulberry.
DOI: 10.1080/09168451.2015.1025691
PubMed: 26024368
Links to Exploration step
pubmed:26024368Le document en format XML
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China. fjao@nwsuaf.edu.cn</nlm:affiliation></affiliation></author><author><name sortKey="Yang, Xiao Bing" sort="Yang, Xiao Bing" uniqKey="Yang X" first="Xiao-Bing" last="Yang">Xiao-Bing Yang</name></author><author><name sortKey="Liu, Li Qun" sort="Liu, Li Qun" uniqKey="Liu L" first="Li-Qun" last="Liu">Li-Qun Liu</name></author><author><name sortKey="Jiang, Tao" sort="Jiang, Tao" uniqKey="Jiang T" first="Tao" last="Jiang">Tao Jiang</name></author><author><name sortKey="Wu, Hai" sort="Wu, Hai" uniqKey="Wu H" first="Hai" last="Wu">Hai Wu</name></author><author><name sortKey="Su, Chao" sort="Su, Chao" uniqKey="Su C" first="Chao" last="Su">Chao Su</name></author><author><name sortKey="Qian, Yong Hua" sort="Qian, Yong Hua" uniqKey="Qian Y" first="Yong-Hua" last="Qian">Yong-Hua Qian</name></author><author><name sortKey="Jiao, Feng" sort="Jiao, Feng" uniqKey="Jiao F" first="Feng" last="Jiao">Feng Jiao</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:26024368</idno><idno type="pmid">26024368</idno><idno type="doi">10.1080/09168451.2015.1025691</idno><idno type="wicri:Area/Main/Corpus">000337</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000337</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Agrobacterium-mediated transient MaFT expression in mulberry (Morus alba L.) leaves.</title><author><name sortKey="Wu, Su Li" sort="Wu, Su Li" uniqKey="Wu S" first="Su-Li" last="Wu">Su-Li Wu</name><affiliation><nlm:affiliation>Institute of Sericulture and Silk , Northwest A&F University, Yangling, P.R. China. fjao@nwsuaf.edu.cn</nlm:affiliation></affiliation></author><author><name sortKey="Yang, Xiao Bing" sort="Yang, Xiao Bing" uniqKey="Yang X" first="Xiao-Bing" last="Yang">Xiao-Bing Yang</name></author><author><name sortKey="Liu, Li Qun" sort="Liu, Li Qun" uniqKey="Liu L" first="Li-Qun" last="Liu">Li-Qun Liu</name></author><author><name sortKey="Jiang, Tao" sort="Jiang, Tao" uniqKey="Jiang T" first="Tao" last="Jiang">Tao Jiang</name></author><author><name sortKey="Wu, Hai" sort="Wu, Hai" uniqKey="Wu H" first="Hai" last="Wu">Hai Wu</name></author><author><name sortKey="Su, Chao" sort="Su, Chao" uniqKey="Su C" first="Chao" last="Su">Chao Su</name></author><author><name sortKey="Qian, Yong Hua" sort="Qian, Yong Hua" uniqKey="Qian Y" first="Yong-Hua" last="Qian">Yong-Hua Qian</name></author><author><name sortKey="Jiao, Feng" sort="Jiao, Feng" uniqKey="Jiao F" first="Feng" last="Jiao">Feng Jiao</name></author></analytic><series><title level="j">Bioscience, biotechnology, and biochemistry</title><idno type="eISSN">1347-6947</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium tumefaciens (genetics)</term><term>Arabidopsis (genetics)</term><term>Arabidopsis (growth & development)</term><term>Arabidopsis Proteins (genetics)</term><term>Fruit (genetics)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Morus (genetics)</term><term>Morus (growth & development)</term><term>Plant Leaves (genetics)</term><term>Plant Leaves (growth & development)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (growth & development)</term><term>Plasmids (MeSH)</term><term>Seedlings (genetics)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Arabidopsis Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agrobacterium tumefaciens</term><term>Arabidopsis</term><term>Fruit</term><term>Morus</term><term>Plant Leaves</term><term>Plants, Genetically Modified</term><term>Seedlings</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Arabidopsis</term><term>Morus</term><term>Plant Leaves</term><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Plant</term><term>Plasmids</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To optimize Agrobacterium-mediated transient transformation assay in mulberry (Morus alba L.), various infiltration methods, Agrobacterium tumefaciens (A. tumefaciens) strains, and bacterial concentrations were tested in mulberry seedlings. Compared with LBA4404, GV3101 harboring pBE2133 plasmids presented stronger GUS signals at 3 days post infiltration using syringe. Recombinant plasmids pBE2133:GFP and pBE2133:GFP:MaFT were successfully constructed. Transient expression of MaFT:GFP protein was found in leaves, petiole (cross section), and shoot apical meristem (SAM) of mulberry according to the GFP signal. Moreover, MaFT:GFP mRNA was also detected in leaves and SAM via RT-PCR and qRT-PCR. An efficient transient transformation system could be achieved in mulberry seedlings by syringe using A. tumefaciens GV3101 at the OD600 of 0.5. The movement of MaFT expression from leaves to SAM might trigger the precocious flowering of mulberry.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26024368</PMID><DateCompleted><Year>2016</Year><Month>04</Month><Day>26</Day></DateCompleted><DateRevised><Year>2015</Year><Month>07</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1347-6947</ISSN><JournalIssue CitedMedium="Internet"><Volume>79</Volume><Issue>8</Issue><PubDate><Year>2015</Year></PubDate></JournalIssue><Title>Bioscience, biotechnology, and biochemistry</Title><ISOAbbreviation>Biosci Biotechnol Biochem</ISOAbbreviation></Journal><ArticleTitle>Agrobacterium-mediated transient MaFT expression in mulberry (Morus alba L.) leaves.</ArticleTitle><Pagination><MedlinePgn>1266-71</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1080/09168451.2015.1025691</ELocationID><Abstract><AbstractText>To optimize Agrobacterium-mediated transient transformation assay in mulberry (Morus alba L.), various infiltration methods, Agrobacterium tumefaciens (A. tumefaciens) strains, and bacterial concentrations were tested in mulberry seedlings. Compared with LBA4404, GV3101 harboring pBE2133 plasmids presented stronger GUS signals at 3 days post infiltration using syringe. Recombinant plasmids pBE2133:GFP and pBE2133:GFP:MaFT were successfully constructed. Transient expression of MaFT:GFP protein was found in leaves, petiole (cross section), and shoot apical meristem (SAM) of mulberry according to the GFP signal. Moreover, MaFT:GFP mRNA was also detected in leaves and SAM via RT-PCR and qRT-PCR. An efficient transient transformation system could be achieved in mulberry seedlings by syringe using A. tumefaciens GV3101 at the OD600 of 0.5. The movement of MaFT expression from leaves to SAM might trigger the precocious flowering of mulberry.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Su-Li</ForeName><Initials>SL</Initials><AffiliationInfo><Affiliation>Institute of Sericulture and Silk , Northwest A&F University, Yangling, P.R. 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