Molecular cloning and characterization of a chlorophyll degradation regulatory gene (ZjSGR) from Zoysia japonica.
Identifieur interne : 000286 ( Main/Corpus ); précédent : 000285; suivant : 000287Molecular cloning and characterization of a chlorophyll degradation regulatory gene (ZjSGR) from Zoysia japonica.
Auteurs : K. Teng ; Z H Chang ; G Z Xiao ; W E Guo ; L X Xu ; Y H Chao ; L B HanSource :
- Genetics and molecular research : GMR [ 1676-5680 ] ; 2016.
English descriptors
- KwdEn :
- Abscisic Acid (metabolism), Chlorophyll (metabolism), Chloroplasts (metabolism), Cloning, Molecular (MeSH), Gene Expression Regulation, Plant (MeSH), Phylogeny (MeSH), Plant Proteins (chemistry), Plant Proteins (genetics), Plant Proteins (metabolism), Poaceae (genetics), Poaceae (metabolism), Protein Transport (MeSH), Stress, Physiological (MeSH).
- MESH :
- chemical , chemistry : Plant Proteins.
- chemical , genetics : Plant Proteins.
- chemical , metabolism : Abscisic Acid, Chlorophyll, Plant Proteins.
- genetics : Poaceae.
- metabolism : Chloroplasts, Poaceae.
- Cloning, Molecular, Gene Expression Regulation, Plant, Phylogeny, Protein Transport, Stress, Physiological.
Abstract
The stay-green gene (SGR) is a key regulatory factor for chlorophyll degradation and senescence. However, to date, little is known about SGR in Zoysia japonica. In this study, ZjSGR was cloned, using rapid amplification of cDNA ends-polymerase chain reaction (PCR). The target sequence is 831 bp in length, corresponding to 276 amino acids. Protein BLAST results showed that ZjSGR belongs to the stay-green superfamily. A phylogenetic analysis implied that ZjSGR is most closely related to ZmSGR1. The subcellular localization of ZjSGR was investigated, using an Agrobacterium-mediated transient expression assay in Nicotiana benthamiana. Our results demonstrated that ZjSGR protein is localized in the chloroplasts. Quantitative real time PCR was carried out to investigate the expression characteristics of ZjSGR. The expression level of ZjSGR was found to be highest in leaves, and could be strongly induced by natural senescence, darkness, abscisic acid (ABA), and methyl jasmonate treatment. Moreover, an in vivo function analysis indicated that transient overexpression of ZjSGR could accelerate chlorophyll degradation, up-regulate the expression of SAG113, and activate ABA biosynthesis. Taken together, these results provide evidence that ZjSGR could play an important regulatory role in leaf chlorophyll degradation and senescence in plants at the molecular level.
DOI: 10.4238/gmr.15028176
PubMed: 27173268
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Molecular cloning and characterization of a chlorophyll degradation regulatory gene (ZjSGR) from Zoysia japonica.</title><author><name sortKey="Teng, K" sort="Teng, K" uniqKey="Teng K" first="K" last="Teng">K. Teng</name><affiliation><nlm:affiliation>Turfgrass Research Institute, College of Forestry, Beijing Forestry University, Beijing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Chang, Z H" sort="Chang, Z H" uniqKey="Chang Z" first="Z H" last="Chang">Z H Chang</name><affiliation><nlm:affiliation>Turfgrass Research Institute, College of Forestry, Beijing Forestry University, Beijing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Xiao, G Z" sort="Xiao, G Z" uniqKey="Xiao G" first="G Z" last="Xiao">G Z Xiao</name><affiliation><nlm:affiliation>The College of Horticulture and Garden, Yangtze University, Jingzhou, China.</nlm:affiliation></affiliation></author><author><name sortKey="Guo, W E" sort="Guo, W E" uniqKey="Guo W" first="W E" last="Guo">W E Guo</name><affiliation><nlm:affiliation>Turfgrass Research Institute, College of Forestry, Beijing Forestry University, Beijing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Xu, L X" sort="Xu, L X" uniqKey="Xu L" first="L X" last="Xu">L X Xu</name><affiliation><nlm:affiliation>Turfgrass Research Institute, College of Forestry, Beijing Forestry University, Beijing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Chao, Y H" sort="Chao, Y H" uniqKey="Chao Y" first="Y H" last="Chao">Y H Chao</name><affiliation><nlm:affiliation>Turfgrass Research Institute, College of Forestry, Beijing Forestry University, Beijing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Han, L B" sort="Han, L B" uniqKey="Han L" first="L B" last="Han">L B Han</name><affiliation><nlm:affiliation>Turfgrass Research Institute, College of Forestry, Beijing Forestry University, Beijing, China.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:27173268</idno><idno type="pmid">27173268</idno><idno type="doi">10.4238/gmr.15028176</idno><idno type="wicri:Area/Main/Corpus">000286</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000286</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Molecular cloning and characterization of a chlorophyll degradation regulatory gene (ZjSGR) from Zoysia japonica.</title><author><name sortKey="Teng, K" sort="Teng, K" uniqKey="Teng K" first="K" last="Teng">K. Teng</name><affiliation><nlm:affiliation>Turfgrass Research Institute, College of Forestry, Beijing Forestry University, Beijing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Chang, Z H" sort="Chang, Z H" uniqKey="Chang Z" first="Z H" last="Chang">Z H Chang</name><affiliation><nlm:affiliation>Turfgrass Research Institute, College of Forestry, Beijing Forestry University, Beijing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Xiao, G Z" sort="Xiao, G Z" uniqKey="Xiao G" first="G Z" last="Xiao">G Z Xiao</name><affiliation><nlm:affiliation>The College of Horticulture and Garden, Yangtze University, Jingzhou, China.</nlm:affiliation></affiliation></author><author><name sortKey="Guo, W E" sort="Guo, W E" uniqKey="Guo W" first="W E" last="Guo">W E Guo</name><affiliation><nlm:affiliation>Turfgrass Research Institute, College of Forestry, Beijing Forestry University, Beijing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Xu, L X" sort="Xu, L X" uniqKey="Xu L" first="L X" last="Xu">L X Xu</name><affiliation><nlm:affiliation>Turfgrass Research Institute, College of Forestry, Beijing Forestry University, Beijing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Chao, Y H" sort="Chao, Y H" uniqKey="Chao Y" first="Y H" last="Chao">Y H Chao</name><affiliation><nlm:affiliation>Turfgrass Research Institute, College of Forestry, Beijing Forestry University, Beijing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Han, L B" sort="Han, L B" uniqKey="Han L" first="L B" last="Han">L B Han</name><affiliation><nlm:affiliation>Turfgrass Research Institute, College of Forestry, Beijing Forestry University, Beijing, China.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Genetics and molecular research : GMR</title><idno type="eISSN">1676-5680</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Abscisic Acid (metabolism)</term><term>Chlorophyll (metabolism)</term><term>Chloroplasts (metabolism)</term><term>Cloning, Molecular (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Phylogeny (MeSH)</term><term>Plant Proteins (chemistry)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Poaceae (genetics)</term><term>Poaceae (metabolism)</term><term>Protein Transport (MeSH)</term><term>Stress, Physiological (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Abscisic Acid</term><term>Chlorophyll</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Poaceae</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Chloroplasts</term><term>Poaceae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cloning, Molecular</term><term>Gene Expression Regulation, Plant</term><term>Phylogeny</term><term>Protein Transport</term><term>Stress, Physiological</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The stay-green gene (SGR) is a key regulatory factor for chlorophyll degradation and senescence. However, to date, little is known about SGR in Zoysia japonica. In this study, ZjSGR was cloned, using rapid amplification of cDNA ends-polymerase chain reaction (PCR). The target sequence is 831 bp in length, corresponding to 276 amino acids. Protein BLAST results showed that ZjSGR belongs to the stay-green superfamily. A phylogenetic analysis implied that ZjSGR is most closely related to ZmSGR1. The subcellular localization of ZjSGR was investigated, using an Agrobacterium-mediated transient expression assay in Nicotiana benthamiana. Our results demonstrated that ZjSGR protein is localized in the chloroplasts. Quantitative real time PCR was carried out to investigate the expression characteristics of ZjSGR. The expression level of ZjSGR was found to be highest in leaves, and could be strongly induced by natural senescence, darkness, abscisic acid (ABA), and methyl jasmonate treatment. Moreover, an in vivo function analysis indicated that transient overexpression of ZjSGR could accelerate chlorophyll degradation, up-regulate the expression of SAG113, and activate ABA biosynthesis. Taken together, these results provide evidence that ZjSGR could play an important regulatory role in leaf chlorophyll degradation and senescence in plants at the molecular level. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27173268</PMID><DateCompleted><Year>2017</Year><Month>02</Month><Day>06</Day></DateCompleted><DateRevised><Year>2017</Year><Month>02</Month><Day>06</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1676-5680</ISSN><JournalIssue CitedMedium="Internet"><Volume>15</Volume><Issue>2</Issue><PubDate><Year>2016</Year><Month>Apr</Month><Day>27</Day></PubDate></JournalIssue><Title>Genetics and molecular research : GMR</Title><ISOAbbreviation>Genet Mol Res</ISOAbbreviation></Journal><ArticleTitle>Molecular cloning and characterization of a chlorophyll degradation regulatory gene (ZjSGR) from Zoysia japonica.</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.4238/gmr.15028176</ELocationID><Abstract><AbstractText>The stay-green gene (SGR) is a key regulatory factor for chlorophyll degradation and senescence. However, to date, little is known about SGR in Zoysia japonica. In this study, ZjSGR was cloned, using rapid amplification of cDNA ends-polymerase chain reaction (PCR). The target sequence is 831 bp in length, corresponding to 276 amino acids. Protein BLAST results showed that ZjSGR belongs to the stay-green superfamily. A phylogenetic analysis implied that ZjSGR is most closely related to ZmSGR1. The subcellular localization of ZjSGR was investigated, using an Agrobacterium-mediated transient expression assay in Nicotiana benthamiana. Our results demonstrated that ZjSGR protein is localized in the chloroplasts. Quantitative real time PCR was carried out to investigate the expression characteristics of ZjSGR. The expression level of ZjSGR was found to be highest in leaves, and could be strongly induced by natural senescence, darkness, abscisic acid (ABA), and methyl jasmonate treatment. Moreover, an in vivo function analysis indicated that transient overexpression of ZjSGR could accelerate chlorophyll degradation, up-regulate the expression of SAG113, and activate ABA biosynthesis. Taken together, these results provide evidence that ZjSGR could play an important regulatory role in leaf chlorophyll degradation and senescence in plants at the molecular level. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Teng</LastName><ForeName>K</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Turfgrass Research Institute, College of Forestry, Beijing Forestry University, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chang</LastName><ForeName>Z H</ForeName><Initials>ZH</Initials><AffiliationInfo><Affiliation>Turfgrass Research Institute, College of Forestry, Beijing Forestry University, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xiao</LastName><ForeName>G Z</ForeName><Initials>GZ</Initials><AffiliationInfo><Affiliation>The College of Horticulture and Garden, Yangtze University, Jingzhou, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guo</LastName><ForeName>W E</ForeName><Initials>WE</Initials><AffiliationInfo><Affiliation>Turfgrass Research Institute, College of Forestry, Beijing Forestry University, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>L X</ForeName><Initials>LX</Initials><AffiliationInfo><Affiliation>Turfgrass Research Institute, College of Forestry, Beijing Forestry University, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chao</LastName><ForeName>Y H</ForeName><Initials>YH</Initials><AffiliationInfo><Affiliation>Turfgrass Research Institute, College of Forestry, Beijing Forestry University, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Han</LastName><ForeName>L B</ForeName><Initials>LB</Initials><AffiliationInfo><Affiliation>Turfgrass Research Institute, College of Forestry, Beijing Forestry University, Beijing, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>04</Month><Day>27</Day></ArticleDate></Article><MedlineJournalInfo><Country>Brazil</Country><MedlineTA>Genet Mol Res</MedlineTA><NlmUniqueID>101169387</NlmUniqueID><ISSNLinking>1676-5680</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>1406-65-1</RegistryNumber><NameOfSubstance UI="D002734">Chlorophyll</NameOfSubstance></Chemical><Chemical><RegistryNumber>72S9A8J5GW</RegistryNumber><NameOfSubstance UI="D000040">Abscisic Acid</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000040" MajorTopicYN="N">Abscisic Acid</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002734" MajorTopicYN="N">Chlorophyll</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002736" MajorTopicYN="N">Chloroplasts</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006109" MajorTopicYN="N">Poaceae</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D021381" MajorTopicYN="N">Protein Transport</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013312" MajorTopicYN="N">Stress, Physiological</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>5</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>5</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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