[Establishment of genetic transformation system for Sophra alopecuroides and deletion analysis of SaLDC promoter].
Identifieur interne : 000204 ( Main/Corpus ); précédent : 000203; suivant : 000205[Establishment of genetic transformation system for Sophra alopecuroides and deletion analysis of SaLDC promoter].
Auteurs : Shan-Shan Wu ; Xiang-Shan Meng ; Juan Li ; Yi Yang ; Ping Liu ; Yan LiuSource :
- Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica [ 1001-5302 ] ; 2017.
English descriptors
- KwdEn :
- Acetophenones (MeSH), Agrobacterium tumefaciens (MeSH), Genes, Reporter (MeSH), Genetic Vectors (MeSH), Glucuronidase (genetics), Plants, Genetically Modified (MeSH), Plants, Medicinal (genetics), Promoter Regions, Genetic (MeSH), Sophora (genetics), Tissue Culture Techniques (MeSH), Transformation, Genetic (MeSH).
- MESH :
- chemical , genetics : Glucuronidase.
- chemical : Acetophenones.
- genetics : Plants, Medicinal, Sophora.
- Agrobacterium tumefaciens, Genes, Reporter, Genetic Vectors, Plants, Genetically Modified, Promoter Regions, Genetic, Tissue Culture Techniques, Transformation, Genetic.
Abstract
Establishing the genetic transformation system of medicinal plant is important to study their functional genes. Based on the established regeneration system of Sophra alopecuroides, 6 factors of genetic transformation were optimized, that was the concentration of Agrobacterium tumefaciens, the infection time, the co-cultivation time of agrobacterium tumefaciensand S.alopecuroides callus, the preculture time of S.alopecuroides callus, the adding method ofacetosyringone (AS) and the concentration of AS, respectively. The results showed that a maximum genetic transformation efficiency of 83.33% was achieved with 15d-precultured of S.alopecuroides callus, which was infected by A600=0.9 A. tumefaciens for 15 minutes and then co-cultivated for 48 hours with 200 μmol•L-1AS. The promoter sequence (1 260 bp) of upstream SaLDC was cloned from S.alopecuroides genomic DNA (gene bank accession number: KY038928). The deletion fragment of SaLDC promoter with different length (310,594,765,924,1 260 bp) were ligated with the GUS reporter gene to form five plant expression vectors named P310,P594,P765,P924,P1260, which were then transferred into S.alopecuroides callus. The GUS transient expression showed that all 5 different deletion fragment of SaLDC promoter can drive the GUS gene expression in S. alopecuroides callus. The SaLDC promoter we cloned has high promoter activity, and they may facilitate its function analysis in the future.
DOI: 10.19540/j.cnki.cjcmm.2017.0076
PubMed: 29090542
Links to Exploration step
pubmed:29090542Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">[Establishment of genetic transformation system for Sophra alopecuroides and deletion analysis of SaLDC promoter].</title><author><name sortKey="Wu, Shan Shan" sort="Wu, Shan Shan" uniqKey="Wu S" first="Shan-Shan" last="Wu">Shan-Shan Wu</name><affiliation><nlm:affiliation>College of Agronomy, Ningxia University, Yinchuan 750021, China.</nlm:affiliation></affiliation></author><author><name sortKey="Meng, Xiang Shan" sort="Meng, Xiang Shan" uniqKey="Meng X" first="Xiang-Shan" last="Meng">Xiang-Shan Meng</name><affiliation><nlm:affiliation>College of Agronomy, Ningxia University, Yinchuan 750021, China.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Juan" sort="Li, Juan" uniqKey="Li J" first="Juan" last="Li">Juan Li</name><affiliation><nlm:affiliation>College of Agronomy, Ningxia University, Yinchuan 750021, China.</nlm:affiliation></affiliation></author><author><name sortKey="Yang, Yi" sort="Yang, Yi" uniqKey="Yang Y" first="Yi" last="Yang">Yi Yang</name><affiliation><nlm:affiliation>College of Agronomy, Ningxia University, Yinchuan 750021, China.</nlm:affiliation></affiliation></author><author><name sortKey="Liu, Ping" sort="Liu, Ping" uniqKey="Liu P" first="Ping" last="Liu">Ping Liu</name><affiliation><nlm:affiliation>College of Agronomy, Ningxia University, Yinchuan 750021, China.</nlm:affiliation></affiliation></author><author><name sortKey="Liu, Yan" sort="Liu, Yan" uniqKey="Liu Y" first="Yan" last="Liu">Yan Liu</name><affiliation><nlm:affiliation>College of Agronomy, Ningxia University, Yinchuan 750021, China.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:29090542</idno><idno type="pmid">29090542</idno><idno type="doi">10.19540/j.cnki.cjcmm.2017.0076</idno><idno type="wicri:Area/Main/Corpus">000204</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000204</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">[Establishment of genetic transformation system for Sophra alopecuroides and deletion analysis of SaLDC promoter].</title><author><name sortKey="Wu, Shan Shan" sort="Wu, Shan Shan" uniqKey="Wu S" first="Shan-Shan" last="Wu">Shan-Shan Wu</name><affiliation><nlm:affiliation>College of Agronomy, Ningxia University, Yinchuan 750021, China.</nlm:affiliation></affiliation></author><author><name sortKey="Meng, Xiang Shan" sort="Meng, Xiang Shan" uniqKey="Meng X" first="Xiang-Shan" last="Meng">Xiang-Shan Meng</name><affiliation><nlm:affiliation>College of Agronomy, Ningxia University, Yinchuan 750021, China.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Juan" sort="Li, Juan" uniqKey="Li J" first="Juan" last="Li">Juan Li</name><affiliation><nlm:affiliation>College of Agronomy, Ningxia University, Yinchuan 750021, China.</nlm:affiliation></affiliation></author><author><name sortKey="Yang, Yi" sort="Yang, Yi" uniqKey="Yang Y" first="Yi" last="Yang">Yi Yang</name><affiliation><nlm:affiliation>College of Agronomy, Ningxia University, Yinchuan 750021, China.</nlm:affiliation></affiliation></author><author><name sortKey="Liu, Ping" sort="Liu, Ping" uniqKey="Liu P" first="Ping" last="Liu">Ping Liu</name><affiliation><nlm:affiliation>College of Agronomy, Ningxia University, Yinchuan 750021, China.</nlm:affiliation></affiliation></author><author><name sortKey="Liu, Yan" sort="Liu, Yan" uniqKey="Liu Y" first="Yan" last="Liu">Yan Liu</name><affiliation><nlm:affiliation>College of Agronomy, Ningxia University, Yinchuan 750021, China.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica</title><idno type="ISSN">1001-5302</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acetophenones (MeSH)</term><term>Agrobacterium tumefaciens (MeSH)</term><term>Genes, Reporter (MeSH)</term><term>Genetic Vectors (MeSH)</term><term>Glucuronidase (genetics)</term><term>Plants, Genetically Modified (MeSH)</term><term>Plants, Medicinal (genetics)</term><term>Promoter Regions, Genetic (MeSH)</term><term>Sophora (genetics)</term><term>Tissue Culture Techniques (MeSH)</term><term>Transformation, Genetic (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Glucuronidase</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Acetophenones</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plants, Medicinal</term><term>Sophora</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Agrobacterium tumefaciens</term><term>Genes, Reporter</term><term>Genetic Vectors</term><term>Plants, Genetically Modified</term><term>Promoter Regions, Genetic</term><term>Tissue Culture Techniques</term><term>Transformation, Genetic</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Establishing the genetic transformation system of medicinal plant is important to study their functional genes. Based on the established regeneration system of Sophra alopecuroides, 6 factors of genetic transformation were optimized, that was the concentration of Agrobacterium tumefaciens, the infection time, the co-cultivation time of agrobacterium tumefaciensand S.alopecuroides callus, the preculture time of S.alopecuroides callus, the adding method ofacetosyringone (AS) and the concentration of AS, respectively. The results showed that a maximum genetic transformation efficiency of 83.33% was achieved with 15d-precultured of S.alopecuroides callus, which was infected by A600=0.9 A. tumefaciens for 15 minutes and then co-cultivated for 48 hours with 200 μmol•L-1AS. The promoter sequence (1 260 bp) of upstream SaLDC was cloned from S.alopecuroides genomic DNA (gene bank accession number: KY038928). The deletion fragment of SaLDC promoter with different length (310,594,765,924,1 260 bp) were ligated with the GUS reporter gene to form five plant expression vectors named P310,P594,P765,P924,P1260, which were then transferred into S.alopecuroides callus. The GUS transient expression showed that all 5 different deletion fragment of SaLDC promoter can drive the GUS gene expression in S. alopecuroides callus. The SaLDC promoter we cloned has high promoter activity, and they may facilitate its function analysis in the future.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">29090542</PMID><DateCompleted><Year>2019</Year><Month>04</Month><Day>15</Day></DateCompleted><DateRevised><Year>2019</Year><Month>04</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1001-5302</ISSN><JournalIssue CitedMedium="Print"><Volume>42</Volume><Issue>10</Issue><PubDate><Year>2017</Year><Month>May</Month></PubDate></JournalIssue><Title>Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica</Title><ISOAbbreviation>Zhongguo Zhong Yao Za Zhi</ISOAbbreviation></Journal><ArticleTitle>[Establishment of genetic transformation system for Sophra alopecuroides and deletion analysis of SaLDC promoter].</ArticleTitle><Pagination><MedlinePgn>1853-1859</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.19540/j.cnki.cjcmm.2017.0076</ELocationID><Abstract><AbstractText>Establishing the genetic transformation system of medicinal plant is important to study their functional genes. Based on the established regeneration system of Sophra alopecuroides, 6 factors of genetic transformation were optimized, that was the concentration of Agrobacterium tumefaciens, the infection time, the co-cultivation time of agrobacterium tumefaciensand S.alopecuroides callus, the preculture time of S.alopecuroides callus, the adding method ofacetosyringone (AS) and the concentration of AS, respectively. The results showed that a maximum genetic transformation efficiency of 83.33% was achieved with 15d-precultured of S.alopecuroides callus, which was infected by A600=0.9 A. tumefaciens for 15 minutes and then co-cultivated for 48 hours with 200 μmol•L-1AS. The promoter sequence (1 260 bp) of upstream SaLDC was cloned from S.alopecuroides genomic DNA (gene bank accession number: KY038928). The deletion fragment of SaLDC promoter with different length (310,594,765,924,1 260 bp) were ligated with the GUS reporter gene to form five plant expression vectors named P310,P594,P765,P924,P1260, which were then transferred into S.alopecuroides callus. The GUS transient expression showed that all 5 different deletion fragment of SaLDC promoter can drive the GUS gene expression in S. alopecuroides callus. The SaLDC promoter we cloned has high promoter activity, and they may facilitate its function analysis in the future.</AbstractText><CopyrightInformation>Copyright© by the Chinese Pharmaceutical Association.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Shan-Shan</ForeName><Initials>SS</Initials><AffiliationInfo><Affiliation>College of Agronomy, Ningxia University, Yinchuan 750021, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Meng</LastName><ForeName>Xiang-Shan</ForeName><Initials>XS</Initials><AffiliationInfo><Affiliation>College of Agronomy, Ningxia University, Yinchuan 750021, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Juan</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>College of Agronomy, Ningxia University, Yinchuan 750021, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Yi</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>College of Agronomy, Ningxia University, Yinchuan 750021, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Ping</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>College of Agronomy, Ningxia University, Yinchuan 750021, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Yan</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>College of Agronomy, Ningxia University, Yinchuan 750021, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>chi</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Zhongguo Zhong Yao Za Zhi</MedlineTA><NlmUniqueID>8913656</NlmUniqueID><ISSNLinking>1001-5302</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000098">Acetophenones</NameOfSubstance></Chemical><Chemical><RegistryNumber>866P45Y84S</RegistryNumber><NameOfSubstance UI="C051667">acetosyringone</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.31</RegistryNumber><NameOfSubstance UI="D005966">Glucuronidase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000098" MajorTopicYN="N">Acetophenones</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016960" MajorTopicYN="N">Agrobacterium tumefaciens</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017930" MajorTopicYN="N">Genes, Reporter</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005822" MajorTopicYN="N">Genetic Vectors</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005966" MajorTopicYN="N">Glucuronidase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010946" MajorTopicYN="N">Plants, Medicinal</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="Y">Promoter Regions, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D029909" MajorTopicYN="N">Sophora</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D046509" MajorTopicYN="N">Tissue Culture Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="Y">Transformation, Genetic</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N"> GUS transient expression </Keyword><Keyword MajorTopicYN="N"> Sophra alopecuroides </Keyword><Keyword MajorTopicYN="N"> function analysis </Keyword><Keyword MajorTopicYN="N"> lysine decarboxylase (LDC) </Keyword><Keyword MajorTopicYN="N"> promoter gene </Keyword></KeywordList><CoiStatement>The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>11</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>11</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>11</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>4</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29090542</ArticleId><ArticleId IdType="doi">10.19540/j.cnki.cjcmm.2017.0076</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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