A transient transformation system for gene characterization in upland cotton (Gossypium hirsutum).
Identifieur interne : 000165 ( Main/Corpus ); précédent : 000164; suivant : 000166A transient transformation system for gene characterization in upland cotton (Gossypium hirsutum).
Auteurs : Haipeng Li ; Kun Li ; Yutao Guo ; Jinggong Guo ; Kaiting Miao ; Jose R. Botella ; Chun-Peng Song ; Yuchen MiaoSource :
- Plant methods [ 1746-4811 ] ; 2018.
Abstract
Background
Genetically modified cotton accounts for 64% of the world's cotton growing area (22.3 million hectares). The genome sequencing of the diploid cotton progenitors
Results
We developed a transient transformation system for gene characterization in upland cotton. Using β-glucuronidase as a reporter for
Conclusions
The
DOI: 10.1186/s13007-018-0319-2
PubMed: 29977323
PubMed Central: PMC6013946
Links to Exploration step
pubmed:29977323Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A transient transformation system for gene characterization in upland cotton (<i>Gossypium hirsutum</i>).</title><author><name sortKey="Li, Haipeng" sort="Li, Haipeng" uniqKey="Li H" first="Haipeng" last="Li">Haipeng Li</name><affiliation><nlm:affiliation>1Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, Department of Biology, Henan University, 85 Minglun Street, Kaifeng, 475001 China.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Kun" sort="Li, Kun" uniqKey="Li K" first="Kun" last="Li">Kun Li</name><affiliation><nlm:affiliation>1Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, Department of Biology, Henan University, 85 Minglun Street, Kaifeng, 475001 China.</nlm:affiliation></affiliation></author><author><name sortKey="Guo, Yutao" sort="Guo, Yutao" uniqKey="Guo Y" first="Yutao" last="Guo">Yutao Guo</name><affiliation><nlm:affiliation>1Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, Department of Biology, Henan University, 85 Minglun Street, Kaifeng, 475001 China.</nlm:affiliation></affiliation></author><author><name sortKey="Guo, Jinggong" sort="Guo, Jinggong" uniqKey="Guo J" first="Jinggong" last="Guo">Jinggong Guo</name><affiliation><nlm:affiliation>1Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, Department of Biology, Henan University, 85 Minglun Street, Kaifeng, 475001 China.</nlm:affiliation></affiliation></author><author><name sortKey="Miao, Kaiting" sort="Miao, Kaiting" uniqKey="Miao K" first="Kaiting" last="Miao">Kaiting Miao</name><affiliation><nlm:affiliation>1Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, Department of Biology, Henan University, 85 Minglun Street, Kaifeng, 475001 China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>2School of Life Science, Southwest University, No. 1, Tiansheng Road, Beibei, Chongqing, 400715 China.</nlm:affiliation></affiliation></author><author><name sortKey="Botella, Jose R" sort="Botella, Jose R" uniqKey="Botella J" first="Jose R" last="Botella">Jose R. Botella</name><affiliation><nlm:affiliation>1Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, Department of Biology, Henan University, 85 Minglun Street, Kaifeng, 475001 China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>3School of Agriculture and Food Sciences, University of Queensland, Brisbane, QLD Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Song, Chun Peng" sort="Song, Chun Peng" uniqKey="Song C" first="Chun-Peng" last="Song">Chun-Peng Song</name><affiliation><nlm:affiliation>1Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, Department of Biology, Henan University, 85 Minglun Street, Kaifeng, 475001 China.</nlm:affiliation></affiliation></author><author><name sortKey="Miao, Yuchen" sort="Miao, Yuchen" uniqKey="Miao Y" first="Yuchen" last="Miao">Yuchen Miao</name><affiliation><nlm:affiliation>1Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, Department of Biology, Henan University, 85 Minglun Street, Kaifeng, 475001 China.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2018">2018</date><idno type="RBID">pubmed:29977323</idno><idno type="pmid">29977323</idno><idno type="doi">10.1186/s13007-018-0319-2</idno><idno type="pmc">PMC6013946</idno><idno type="wicri:Area/Main/Corpus">000165</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000165</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A transient transformation system for gene characterization in upland cotton (<i>Gossypium hirsutum</i>).</title><author><name sortKey="Li, Haipeng" sort="Li, Haipeng" uniqKey="Li H" first="Haipeng" last="Li">Haipeng Li</name><affiliation><nlm:affiliation>1Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, Department of Biology, Henan University, 85 Minglun Street, Kaifeng, 475001 China.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Kun" sort="Li, Kun" uniqKey="Li K" first="Kun" last="Li">Kun Li</name><affiliation><nlm:affiliation>1Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, Department of Biology, Henan University, 85 Minglun Street, Kaifeng, 475001 China.</nlm:affiliation></affiliation></author><author><name sortKey="Guo, Yutao" sort="Guo, Yutao" uniqKey="Guo Y" first="Yutao" last="Guo">Yutao Guo</name><affiliation><nlm:affiliation>1Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, Department of Biology, Henan University, 85 Minglun Street, Kaifeng, 475001 China.</nlm:affiliation></affiliation></author><author><name sortKey="Guo, Jinggong" sort="Guo, Jinggong" uniqKey="Guo J" first="Jinggong" last="Guo">Jinggong Guo</name><affiliation><nlm:affiliation>1Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, Department of Biology, Henan University, 85 Minglun Street, Kaifeng, 475001 China.</nlm:affiliation></affiliation></author><author><name sortKey="Miao, Kaiting" sort="Miao, Kaiting" uniqKey="Miao K" first="Kaiting" last="Miao">Kaiting Miao</name><affiliation><nlm:affiliation>1Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, Department of Biology, Henan University, 85 Minglun Street, Kaifeng, 475001 China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>2School of Life Science, Southwest University, No. 1, Tiansheng Road, Beibei, Chongqing, 400715 China.</nlm:affiliation></affiliation></author><author><name sortKey="Botella, Jose R" sort="Botella, Jose R" uniqKey="Botella J" first="Jose R" last="Botella">Jose R. Botella</name><affiliation><nlm:affiliation>1Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, Department of Biology, Henan University, 85 Minglun Street, Kaifeng, 475001 China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>3School of Agriculture and Food Sciences, University of Queensland, Brisbane, QLD Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Song, Chun Peng" sort="Song, Chun Peng" uniqKey="Song C" first="Chun-Peng" last="Song">Chun-Peng Song</name><affiliation><nlm:affiliation>1Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, Department of Biology, Henan University, 85 Minglun Street, Kaifeng, 475001 China.</nlm:affiliation></affiliation></author><author><name sortKey="Miao, Yuchen" sort="Miao, Yuchen" uniqKey="Miao Y" first="Yuchen" last="Miao">Yuchen Miao</name><affiliation><nlm:affiliation>1Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, Department of Biology, Henan University, 85 Minglun Street, Kaifeng, 475001 China.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Plant methods</title><idno type="ISSN">1746-4811</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>Background</b></p><p>Genetically modified cotton accounts for 64% of the world's cotton growing area (22.3 million hectares). The genome sequencing of the diploid cotton progenitors </p></div><div type="abstract" xml:lang="en"><p><b>Results</b></p><p>We developed a transient transformation system for gene characterization in upland cotton. Using β-glucuronidase as a reporter for </p></div><div type="abstract" xml:lang="en"><p><b>Conclusions</b></p><p>The </p></div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">29977323</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>01</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1746-4811</ISSN><JournalIssue CitedMedium="Print"><Volume>14</Volume><PubDate><Year>2018</Year></PubDate></JournalIssue><Title>Plant methods</Title><ISOAbbreviation>Plant Methods</ISOAbbreviation></Journal><ArticleTitle>A transient transformation system for gene characterization in upland cotton (<i>Gossypium hirsutum</i>).</ArticleTitle><Pagination><MedlinePgn>50</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/s13007-018-0319-2</ELocationID><Abstract><AbstractText Label="Background" NlmCategory="UNASSIGNED">Genetically modified cotton accounts for 64% of the world's cotton growing area (22.3 million hectares). The genome sequencing of the diploid cotton progenitors <i>Gossypium raimondii</i> and <i>Gossypium arboreum</i> as well as the cultivated <i>Gossypium hirsutum</i> has provided a wealth of genetic information that could be exploited for crop improvement. Unfortunately, gene functional characterization in cotton is lagging behind other economically important crops due to the low efficiency, lengthiness and technical complexity of the available stable transformation methods. We present here a simple, fast and efficient method for the transient transformation of <i>G</i>. <i>hirsutum</i> that can be used for gene characterization studies.</AbstractText><AbstractText Label="Results" NlmCategory="UNASSIGNED">We developed a transient transformation system for gene characterization in upland cotton. Using β-glucuronidase as a reporter for <i>Agrobacterium</i>-mediated transformation assays, we evaluated multiple transformation parameters such as <i>Agrobacterium</i> strain, bacterial density, length of co-cultivation, chemicals and surfactants, which can affect transformation efficiency. After the initial characterization, the <i>Agrobacterium</i> EHA105 strain was selected and a number of binary constructs used to perform gene characterization studies. 7-days-old cotton seedlings were co-cultivated with <i>Agrobacterium</i> and transient gene expression was observed 5 days after infection of the plants. Transcript levels of two different transgenes under the control of the cauliflower mosaic virus (CaMV) 35S promoter were quantified by real-time reverse transcription PCR (qRT-PCR) showing a 3-10 times increase over the levels observed in non-infected controls. The expression patterns driven by the promoters of two <i>G</i>. <i>hirsutum</i> genes as well as the subcellular localization of their corresponding proteins were studied using the new transient expression system and our observations were consistent with previously published results using <i>Arabidopsis</i> as a heterologous system.</AbstractText><AbstractText Label="Conclusions" NlmCategory="UNASSIGNED">The <i>Agrobacterium</i>-mediated transient transformation method is a fast and easy transient expression system enabling high transient expression and transformation efficiency in upland cotton seedlings. Our method can be used for gene functional studies such as promoter characterization and protein subcellular localization in cotton, obviating the need to perform such studies in a heterologous system such as <i>Arabidopsis</i>.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y" EqualContrib="Y"><LastName>Li</LastName><ForeName>Haipeng</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>1Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, Department of Biology, Henan University, 85 Minglun Street, Kaifeng, 475001 China.</Affiliation><Identifier Source="ISNI">0000 0000 9139 560X</Identifier><Identifier Source="GRID">grid.256922.8</Identifier></AffiliationInfo></Author><Author ValidYN="Y" EqualContrib="Y"><LastName>Li</LastName><ForeName>Kun</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>1Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, Department of Biology, Henan University, 85 Minglun Street, Kaifeng, 475001 China.</Affiliation><Identifier Source="ISNI">0000 0000 9139 560X</Identifier><Identifier Source="GRID">grid.256922.8</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guo</LastName><ForeName>Yutao</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>1Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, Department of Biology, Henan University, 85 Minglun Street, Kaifeng, 475001 China.</Affiliation><Identifier Source="ISNI">0000 0000 9139 560X</Identifier><Identifier Source="GRID">grid.256922.8</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guo</LastName><ForeName>Jinggong</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>1Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, Department of Biology, Henan University, 85 Minglun Street, Kaifeng, 475001 China.</Affiliation><Identifier Source="ISNI">0000 0000 9139 560X</Identifier><Identifier Source="GRID">grid.256922.8</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Miao</LastName><ForeName>Kaiting</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>1Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, Department of Biology, Henan University, 85 Minglun Street, Kaifeng, 475001 China.</Affiliation><Identifier Source="ISNI">0000 0000 9139 560X</Identifier><Identifier Source="GRID">grid.256922.8</Identifier></AffiliationInfo><AffiliationInfo><Affiliation>2School of Life Science, Southwest University, No. 1, Tiansheng Road, Beibei, Chongqing, 400715 China.</Affiliation><Identifier Source="GRID">grid.263906.8</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Botella</LastName><ForeName>Jose R</ForeName><Initials>JR</Initials><AffiliationInfo><Affiliation>1Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, Department of Biology, Henan University, 85 Minglun Street, Kaifeng, 475001 China.</Affiliation><Identifier Source="ISNI">0000 0000 9139 560X</Identifier><Identifier Source="GRID">grid.256922.8</Identifier></AffiliationInfo><AffiliationInfo><Affiliation>3School of Agriculture and Food Sciences, University of Queensland, Brisbane, QLD Australia.</Affiliation><Identifier Source="ISNI">0000 0000 9320 7537</Identifier><Identifier Source="GRID">grid.1003.2</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Song</LastName><ForeName>Chun-Peng</ForeName><Initials>CP</Initials><AffiliationInfo><Affiliation>1Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, Department of Biology, Henan University, 85 Minglun Street, Kaifeng, 475001 China.</Affiliation><Identifier Source="ISNI">0000 0000 9139 560X</Identifier><Identifier Source="GRID">grid.256922.8</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Miao</LastName><ForeName>Yuchen</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>1Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, Department of Biology, Henan University, 85 Minglun Street, Kaifeng, 475001 China.</Affiliation><Identifier Source="ISNI">0000 0000 9139 560X</Identifier><Identifier Source="GRID">grid.256922.8</Identifier></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>06</Month><Day>22</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Plant Methods</MedlineTA><NlmUniqueID>101245798</NlmUniqueID><ISSNLinking>1746-4811</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Agrobacterium</Keyword><Keyword MajorTopicYN="N">Cotton</Keyword><Keyword MajorTopicYN="N">Gene characterization</Keyword><Keyword MajorTopicYN="N">Gossypium hirsutum</Keyword><Keyword MajorTopicYN="N">Transient transformation</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>02</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>06</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>7</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>7</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>7</Month><Day>7</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29977323</ArticleId><ArticleId IdType="doi">10.1186/s13007-018-0319-2</ArticleId><ArticleId IdType="pii">319</ArticleId><ArticleId IdType="pmc">PMC6013946</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Sci Rep. 2017 Mar 13;7:44304</Citation><ArticleIdList><ArticleId IdType="pubmed">28287154</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 2015 May;33(5):524-30</Citation><ArticleIdList><ArticleId IdType="pubmed">25893780</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods Mol Biol. 2013;958:3-15</Citation><ArticleIdList><ArticleId IdType="pubmed">23143479</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2017 Feb;173(2):1391-1408</Citation><ArticleIdList><ArticleId IdType="pubmed">27913741</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Adv. 2009 Nov-Dec;27(6):753-63</Citation><ArticleIdList><ArticleId IdType="pubmed">19508888</ArticleId></ArticleIdList></Reference><Reference><Citation>Sci Rep. 2016 Feb 09;6:20773</Citation><ArticleIdList><ArticleId IdType="pubmed">26856318</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Methods. 2014 Jun 18;10:19</Citation><ArticleIdList><ArticleId IdType="pubmed">24987449</ArticleId></ArticleIdList></Reference><Reference><Citation>J Vis Exp. 2011 Aug 20;(54):null</Citation><ArticleIdList><ArticleId IdType="pubmed">21876527</ArticleId></ArticleIdList></Reference><Reference><Citation>J Gen Virol. 2013 Apr;94(Pt 4):695-710</Citation><ArticleIdList><ArticleId IdType="pubmed">23324471</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Biotechnol. 2016 Jan;58(1):65-72</Citation><ArticleIdList><ArticleId IdType="pubmed">26603442</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 2015 May;33(5):531-7</Citation><ArticleIdList><ArticleId IdType="pubmed">25893781</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 1987 Dec 20;6(13):3901-7</Citation><ArticleIdList><ArticleId IdType="pubmed">3327686</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2013 Sep 02;8(9):e73211</Citation><ArticleIdList><ArticleId IdType="pubmed">24023833</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Adv. 2015 Nov 1;33(6 Pt 2):1024-42</Citation><ArticleIdList><ArticleId IdType="pubmed">25819757</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 2017 Jul;94(4-5):349-360</Citation><ArticleIdList><ArticleId IdType="pubmed">28258551</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Plant Sci. 2017 Aug 03;8:1364</Citation><ArticleIdList><ArticleId IdType="pubmed">28824692</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2006 May 16;103(20):7571-6</Citation><ArticleIdList><ArticleId IdType="pubmed">16675554</ArticleId></ArticleIdList></Reference><Reference><Citation>Sci Rep. 2017 Mar 03;7:43902</Citation><ArticleIdList><ArticleId IdType="pubmed">28256588</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods Mol Biol. 2013;975:157-65</Citation><ArticleIdList><ArticleId IdType="pubmed">23386302</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2005 Aug;17(8):2384-96</Citation><ArticleIdList><ArticleId IdType="pubmed">15994908</ArticleId></ArticleIdList></Reference><Reference><Citation>Int J Dev Biol. 2013;57(6-8):467-81</Citation><ArticleIdList><ArticleId IdType="pubmed">24166430</ArticleId></ArticleIdList></Reference><Reference><Citation>Sci Rep. 2017 Mar 16;7:44743</Citation><ArticleIdList><ArticleId IdType="pubmed">28300195</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 1990 Jan;14(1):61-72</Citation><ArticleIdList><ArticleId IdType="pubmed">2101312</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 1987 Mar;10(2):105-16</Citation><ArticleIdList><ArticleId IdType="pubmed">24277496</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Plant Sci. 2015 Oct 31;6:838</Citation><ArticleIdList><ArticleId IdType="pubmed">26583018</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2014 Apr 18;9(4):e93577</Citation><ArticleIdList><ArticleId IdType="pubmed">24747610</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Biotechnol J. 2018 Jan;16(1):137-150</Citation><ArticleIdList><ArticleId IdType="pubmed">28499063</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/AgrobacTransV1/Data/Main/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000165 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd -nk 000165 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= AgrobacTransV1
|flux= Main
|étape= Corpus
|type= RBID
|clé= pubmed:29977323
|texte= A transient transformation system for gene characterization in upland cotton (Gossypium hirsutum).
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Corpus/RBID.i -Sk "pubmed:29977323" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a AgrobacTransV1
| This area was generated with Dilib version V0.6.38. |  |