Rapid validation of transcriptional enhancers using agrobacterium-mediated transient assay.
Identifieur interne : 000118 ( Main/Corpus ); précédent : 000117; suivant : 000119Rapid validation of transcriptional enhancers using agrobacterium-mediated transient assay.
Auteurs : Yuan Lin ; Fanli Meng ; Chao Fang ; Bo Zhu ; Jiming JiangSource :
- Plant methods [ 1746-4811 ] ; 2019.
Abstract
Background
Enhancers are one of the most important classes of
Results
We developed a rapid enhancer validation system based on
Conclusions
In summary, we developed a rapid and efficient plant enhancer validation system based on a luciferase reporter and
DOI: 10.1186/s13007-019-0407-y
PubMed: 30873216
PubMed Central: PMC6402126
Links to Exploration step
pubmed:30873216Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Rapid validation of transcriptional enhancers using agrobacterium-mediated transient assay.</title>
<author><name sortKey="Lin, Yuan" sort="Lin, Yuan" uniqKey="Lin Y" first="Yuan" last="Lin">Yuan Lin</name>
<affiliation><nlm:affiliation>1Department of Plant Biology, Michigan State University, East Lansing, MI 48824 USA.</nlm:affiliation>
</affiliation>
<affiliation><nlm:affiliation>2Department of Horticulture, Michigan State University, East Lansing, MI 48824 USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Meng, Fanli" sort="Meng, Fanli" uniqKey="Meng F" first="Fanli" last="Meng">Fanli Meng</name>
<affiliation><nlm:affiliation>3Key Laboratory of Soybean Biology in Chinese Ministry of Education, Northeast Agricultural University, Harbin, 150030 China.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Fang, Chao" sort="Fang, Chao" uniqKey="Fang C" first="Chao" last="Fang">Chao Fang</name>
<affiliation><nlm:affiliation>1Department of Plant Biology, Michigan State University, East Lansing, MI 48824 USA.</nlm:affiliation>
</affiliation>
<affiliation><nlm:affiliation>2Department of Horticulture, Michigan State University, East Lansing, MI 48824 USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Zhu, Bo" sort="Zhu, Bo" uniqKey="Zhu B" first="Bo" last="Zhu">Bo Zhu</name>
<affiliation><nlm:affiliation>4Department of Biological Science, College of Life Sciences, Sichuan Normal University, Chengdu, Sichuan 610101 China.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Jiang, Jiming" sort="Jiang, Jiming" uniqKey="Jiang J" first="Jiming" last="Jiang">Jiming Jiang</name>
<affiliation><nlm:affiliation>1Department of Plant Biology, Michigan State University, East Lansing, MI 48824 USA.</nlm:affiliation>
</affiliation>
<affiliation><nlm:affiliation>2Department of Horticulture, Michigan State University, East Lansing, MI 48824 USA.</nlm:affiliation>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2019">2019</date>
<idno type="RBID">pubmed:30873216</idno>
<idno type="pmid">30873216</idno>
<idno type="doi">10.1186/s13007-019-0407-y</idno>
<idno type="pmc">PMC6402126</idno>
<idno type="wicri:Area/Main/Corpus">000118</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000118</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Rapid validation of transcriptional enhancers using agrobacterium-mediated transient assay.</title>
<author><name sortKey="Lin, Yuan" sort="Lin, Yuan" uniqKey="Lin Y" first="Yuan" last="Lin">Yuan Lin</name>
<affiliation><nlm:affiliation>1Department of Plant Biology, Michigan State University, East Lansing, MI 48824 USA.</nlm:affiliation>
</affiliation>
<affiliation><nlm:affiliation>2Department of Horticulture, Michigan State University, East Lansing, MI 48824 USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Meng, Fanli" sort="Meng, Fanli" uniqKey="Meng F" first="Fanli" last="Meng">Fanli Meng</name>
<affiliation><nlm:affiliation>3Key Laboratory of Soybean Biology in Chinese Ministry of Education, Northeast Agricultural University, Harbin, 150030 China.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Fang, Chao" sort="Fang, Chao" uniqKey="Fang C" first="Chao" last="Fang">Chao Fang</name>
<affiliation><nlm:affiliation>1Department of Plant Biology, Michigan State University, East Lansing, MI 48824 USA.</nlm:affiliation>
</affiliation>
<affiliation><nlm:affiliation>2Department of Horticulture, Michigan State University, East Lansing, MI 48824 USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Zhu, Bo" sort="Zhu, Bo" uniqKey="Zhu B" first="Bo" last="Zhu">Bo Zhu</name>
<affiliation><nlm:affiliation>4Department of Biological Science, College of Life Sciences, Sichuan Normal University, Chengdu, Sichuan 610101 China.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Jiang, Jiming" sort="Jiang, Jiming" uniqKey="Jiang J" first="Jiming" last="Jiang">Jiming Jiang</name>
<affiliation><nlm:affiliation>1Department of Plant Biology, Michigan State University, East Lansing, MI 48824 USA.</nlm:affiliation>
</affiliation>
<affiliation><nlm:affiliation>2Department of Horticulture, Michigan State University, East Lansing, MI 48824 USA.</nlm:affiliation>
</affiliation>
</author>
</analytic>
<series><title level="j">Plant methods</title>
<idno type="ISSN">1746-4811</idno>
<imprint><date when="2019" type="published">2019</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p><b>Background</b>
</p>
<p>Enhancers are one of the most important classes of </p>
</div>
<div type="abstract" xml:lang="en"><p><b>Results</b>
</p>
<p>We developed a rapid enhancer validation system based on </p>
</div>
<div type="abstract" xml:lang="en"><p><b>Conclusions</b>
</p>
<p>In summary, we developed a rapid and efficient plant enhancer validation system based on a luciferase reporter and </p>
</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">30873216</PMID>
<DateRevised><Year>2020</Year>
<Month>10</Month>
<Day>01</Day>
</DateRevised>
<Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1746-4811</ISSN>
<JournalIssue CitedMedium="Print"><Volume>15</Volume>
<PubDate><Year>2019</Year>
</PubDate>
</JournalIssue>
<Title>Plant methods</Title>
<ISOAbbreviation>Plant Methods</ISOAbbreviation>
</Journal>
<ArticleTitle>Rapid validation of transcriptional enhancers using agrobacterium-mediated transient assay.</ArticleTitle>
<Pagination><MedlinePgn>21</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1186/s13007-019-0407-y</ELocationID>
<Abstract><AbstractText Label="Background" NlmCategory="UNASSIGNED">Enhancers are one of the most important classes of <i>cis</i>
-regulatory elements (CREs) and play key roles in regulation of transcription in higher eukaryotes. Enhancers are difficult to identify because they lack positional constraints relative to their cognate genes. Excitingly, several recent studies showed that plant enhancers can be predicted based on their distinct features associated with open chromatin. However, experimental validation is necessary to confirm the predicted enhancer function.</AbstractText>
<AbstractText Label="Results" NlmCategory="UNASSIGNED">We developed a rapid enhancer validation system based on <i>Nicotiana benthamiana</i>
. A set of 12 intergenic and intronic enhancers, identified in <i>Arabidopsis thaliana</i>
, were cloned into a vector containing a minimal 35S promoter and a luciferase reporter gene, and were then infiltrated into <i>N. benthamiana</i>
leaves mediated by agrobacterium. The enhancer activity of each candidate was quantitatively assayed based on bioluminescence measurement. The data from this luciferase-based validation was correlated with previous data derived from transgenic assays in <i>A. thaliana</i>
. In addition, the relative strength of different enhancers for driving the reporter gene can be quantitatively compared. We demonstrate that this system can also be used to map the functional activity of a candidate enhancer under different environmental conditions.</AbstractText>
<AbstractText Label="Conclusions" NlmCategory="UNASSIGNED">In summary, we developed a rapid and efficient plant enhancer validation system based on a luciferase reporter and <i>N. benthamiana</i>
-based leaf agroinfiltration. This system can be used for initial screening of leaf-specific enhancers and for validating candidate leaf enhancers from dicot species. It can potentially be used to examine the activity of candidate enhancers under different environmental conditions.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lin</LastName>
<ForeName>Yuan</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>1Department of Plant Biology, Michigan State University, East Lansing, MI 48824 USA.</Affiliation>
<Identifier Source="ISNI">0000 0001 2150 1785</Identifier>
<Identifier Source="GRID">grid.17088.36</Identifier>
</AffiliationInfo>
<AffiliationInfo><Affiliation>2Department of Horticulture, Michigan State University, East Lansing, MI 48824 USA.</Affiliation>
<Identifier Source="ISNI">0000 0001 2150 1785</Identifier>
<Identifier Source="GRID">grid.17088.36</Identifier>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Meng</LastName>
<ForeName>Fanli</ForeName>
<Initials>F</Initials>
<AffiliationInfo><Affiliation>3Key Laboratory of Soybean Biology in Chinese Ministry of Education, Northeast Agricultural University, Harbin, 150030 China.</Affiliation>
<Identifier Source="ISNI">0000 0004 1760 1136</Identifier>
<Identifier Source="GRID">grid.412243.2</Identifier>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Fang</LastName>
<ForeName>Chao</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>1Department of Plant Biology, Michigan State University, East Lansing, MI 48824 USA.</Affiliation>
<Identifier Source="ISNI">0000 0001 2150 1785</Identifier>
<Identifier Source="GRID">grid.17088.36</Identifier>
</AffiliationInfo>
<AffiliationInfo><Affiliation>2Department of Horticulture, Michigan State University, East Lansing, MI 48824 USA.</Affiliation>
<Identifier Source="ISNI">0000 0001 2150 1785</Identifier>
<Identifier Source="GRID">grid.17088.36</Identifier>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zhu</LastName>
<ForeName>Bo</ForeName>
<Initials>B</Initials>
<AffiliationInfo><Affiliation>4Department of Biological Science, College of Life Sciences, Sichuan Normal University, Chengdu, Sichuan 610101 China.</Affiliation>
<Identifier Source="ISNI">0000 0000 9479 9538</Identifier>
<Identifier Source="GRID">grid.412600.1</Identifier>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Jiang</LastName>
<ForeName>Jiming</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>1Department of Plant Biology, Michigan State University, East Lansing, MI 48824 USA.</Affiliation>
<Identifier Source="ISNI">0000 0001 2150 1785</Identifier>
<Identifier Source="GRID">grid.17088.36</Identifier>
</AffiliationInfo>
<AffiliationInfo><Affiliation>2Department of Horticulture, Michigan State University, East Lansing, MI 48824 USA.</Affiliation>
<Identifier Source="ISNI">0000 0001 2150 1785</Identifier>
<Identifier Source="GRID">grid.17088.36</Identifier>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2019</Year>
<Month>03</Month>
<Day>06</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>Plant Methods</MedlineTA>
<NlmUniqueID>101245798</NlmUniqueID>
<ISSNLinking>1746-4811</ISSNLinking>
</MedlineJournalInfo>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Enhancer validation</Keyword>
<Keyword MajorTopicYN="N">Luciferase</Keyword>
<Keyword MajorTopicYN="N">Nicotiana benthamiana</Keyword>
<Keyword MajorTopicYN="N">Transient assay</Keyword>
</KeywordList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year>
<Month>09</Month>
<Day>10</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2019</Year>
<Month>02</Month>
<Day>28</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2019</Year>
<Month>3</Month>
<Day>16</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2019</Year>
<Month>3</Month>
<Day>16</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2019</Year>
<Month>3</Month>
<Day>16</Day>
<Hour>6</Hour>
<Minute>1</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>epublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">30873216</ArticleId>
<ArticleId IdType="doi">10.1186/s13007-019-0407-y</ArticleId>
<ArticleId IdType="pii">407</ArticleId>
<ArticleId IdType="pmc">PMC6402126</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>Mol Plant Microbe Interact. 2000 Apr;13(4):439-46</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10755307</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2001 Aug;126(4):1630-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11500561</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2003 Aug;35(3):418-27</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12887592</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 1992 Sep;4(9):1075-87</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1392609</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2004 Apr;134(4):1742-51</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15051863</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2004 May;16(5):1077-90</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15100398</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biotechnol Bioeng. 2004 Jun 30;86(7):827-34</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15162459</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Biochim Pol. 2006;53(2):289-98</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16582986</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2006 Apr;18(4):792-803</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16595397</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Methods Mol Biol. 2006;323:225-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16739580</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Biotechnol J. 2005 Mar;3(2):259-73</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17173625</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 1986 Nov 14;234(4778):856-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17758108</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biosci. 2008 Sep;33(3):337-43</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19005233</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2009 Jun 05;4(6):e5812</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19503835</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Chem Biol. 2010 Jun 25;17(6):646-57</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20609414</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Methods Mol Biol. 2011;680:3-28</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21153370</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>CSH Protoc. 2007 Feb 01;2007:pdb.prot4690</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21357025</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2012 Feb;69(4):713-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22004025</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genome Res. 2012 Jan;22(1):151-62</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22110044</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2012 Jul;24(7):2719-31</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22773751</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Genet. 2013;9(7):e1003625</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23874224</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell Rep. 1990 Jun;9(1):1-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24226366</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Rev Genet. 2014 Apr;15(4):272-86</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24614317</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2014 Mar 27;507(7493):455-461</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24670763</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Signal Behav. 2014 Apr 30;9:null</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24785194</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Plant. 2015 Jul;8(7):998-1010</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25620770</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2015 Sep;27(9):2415-26</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26373455</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Rev Genet. 2016 Apr;17(4):207-23</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26948815</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochim Biophys Acta Gene Regul Mech. 2017 Jan;1860(1):131-139</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27321818</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Plant Biol. 2016 Oct;33:72-76</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27344391</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Trends Plant Sci. 2016 Nov;21(11):974-987</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27593567</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>New Phytol. 2017 Oct;216(2):576-590</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28244104</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Biotechnol J. 2017 Nov;15(11):1439-1452</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28317264</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biotechnol Lett. 2017 Jul;39(7):1059-1067</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28439740</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genome Biol. 2017 Jul 21;18(1):137</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28732548</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2018 Apr;176(4):2789-2803</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29463772</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>EMBO J. 1987 Dec 20;6(13):3901-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3327686</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 1985 Dec;82(23):7870-3</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3906652</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell. 1981 Dec;27(2 Pt 1):299-308</Citation>
<ArticleIdList><ArticleId IdType="pubmed">6277502</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genes Dev. 1995 Jul 15;9(14):1797-810</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7622040</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 1996 Oct;10(4):745-54</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8893550</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/AgrobacTransV1/Data/Main/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000118 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd -nk 000118 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Bois |area= AgrobacTransV1 |flux= Main |étape= Corpus |type= RBID |clé= pubmed:30873216 |texte= Rapid validation of transcriptional enhancers using agrobacterium-mediated transient assay. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Corpus/RBID.i -Sk "pubmed:30873216" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd \ | NlmPubMed2Wicri -a AgrobacTransV1
![]() | This area was generated with Dilib version V0.6.38. | ![]() |