Plant based production of myoglobin - a novel source of the muscle heme-protein.
Identifieur interne : 000062 ( Main/Corpus ); précédent : 000061; suivant : 000063Plant based production of myoglobin - a novel source of the muscle heme-protein.
Auteurs : Magnus L R. Carlsson ; Selvaraju Kanagarajan ; Leif Bülow ; Li-Hua ZhuSource :
- Scientific reports [ 2045-2322 ] ; 2020.
Abstract
Myoglobin is a heme-protein in the muscle of vertebrates with important functions in the oxygenation of tissues and as a regulator in nitric oxide signaling. Myoglobin from many species is also an important nutritional source of bioavailable iron. In this study, we have successfully produced human myoglobin in the leaves of Nicotiana benthamiana by transient expression using a viral vector delivered by Agrobacterium tumefaciens. Analyses confirmed that heme was incorporated and the protein was functional, with observed properties consistent with those of native myoglobins. A relatively high degree of purity could be achieved with low cost methods. The results show the high potential of plants as a production platform for heme proteins, a group of proteins of interest for iron nutrition applications and possible future pharmaceutical development.
DOI: 10.1038/s41598-020-57565-y
PubMed: 31969582
PubMed Central: PMC6976567
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Carlsson</name><affiliation><nlm:affiliation>Department of Plant Breeding, Swedish University of Agricultural Sciences, Box 101, 230 53, Alnarp, Sweden.</nlm:affiliation></affiliation></author><author><name sortKey="Kanagarajan, Selvaraju" sort="Kanagarajan, Selvaraju" uniqKey="Kanagarajan S" first="Selvaraju" last="Kanagarajan">Selvaraju Kanagarajan</name><affiliation><nlm:affiliation>Department of Plant Breeding, Swedish University of Agricultural Sciences, Box 101, 230 53, Alnarp, Sweden.</nlm:affiliation></affiliation></author><author><name sortKey="Bulow, Leif" sort="Bulow, Leif" uniqKey="Bulow L" first="Leif" last="Bülow">Leif Bülow</name><affiliation><nlm:affiliation>Division of Pure and Applied Biochemistry, Department of Chemistry, Lund University, Box 124, 221 00, Lund, Sweden.</nlm:affiliation></affiliation></author><author><name sortKey="Zhu, Li Hua" sort="Zhu, Li Hua" uniqKey="Zhu L" first="Li-Hua" last="Zhu">Li-Hua Zhu</name><affiliation><nlm:affiliation>Department of Plant Breeding, Swedish University of Agricultural Sciences, Box 101, 230 53, Alnarp, Sweden. Li-Hua.Zhu@slu.se.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:31969582</idno><idno type="pmid">31969582</idno><idno type="doi">10.1038/s41598-020-57565-y</idno><idno type="pmc">PMC6976567</idno><idno type="wicri:Area/Main/Corpus">000062</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000062</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Plant based production of myoglobin - a novel source of the muscle heme-protein.</title><author><name sortKey="Carlsson, Magnus L R" sort="Carlsson, Magnus L R" uniqKey="Carlsson M" first="Magnus L R" last="Carlsson">Magnus L R. Carlsson</name><affiliation><nlm:affiliation>Department of Plant Breeding, Swedish University of Agricultural Sciences, Box 101, 230 53, Alnarp, Sweden.</nlm:affiliation></affiliation></author><author><name sortKey="Kanagarajan, Selvaraju" sort="Kanagarajan, Selvaraju" uniqKey="Kanagarajan S" first="Selvaraju" last="Kanagarajan">Selvaraju Kanagarajan</name><affiliation><nlm:affiliation>Department of Plant Breeding, Swedish University of Agricultural Sciences, Box 101, 230 53, Alnarp, Sweden.</nlm:affiliation></affiliation></author><author><name sortKey="Bulow, Leif" sort="Bulow, Leif" uniqKey="Bulow L" first="Leif" last="Bülow">Leif Bülow</name><affiliation><nlm:affiliation>Division of Pure and Applied Biochemistry, Department of Chemistry, Lund University, Box 124, 221 00, Lund, Sweden.</nlm:affiliation></affiliation></author><author><name sortKey="Zhu, Li Hua" sort="Zhu, Li Hua" uniqKey="Zhu L" first="Li-Hua" last="Zhu">Li-Hua Zhu</name><affiliation><nlm:affiliation>Department of Plant Breeding, Swedish University of Agricultural Sciences, Box 101, 230 53, Alnarp, Sweden. Li-Hua.Zhu@slu.se.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Scientific reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Myoglobin is a heme-protein in the muscle of vertebrates with important functions in the oxygenation of tissues and as a regulator in nitric oxide signaling. Myoglobin from many species is also an important nutritional source of bioavailable iron. In this study, we have successfully produced human myoglobin in the leaves of Nicotiana benthamiana by transient expression using a viral vector delivered by Agrobacterium tumefaciens. Analyses confirmed that heme was incorporated and the protein was functional, with observed properties consistent with those of native myoglobins. A relatively high degree of purity could be achieved with low cost methods. The results show the high potential of plants as a production platform for heme proteins, a group of proteins of interest for iron nutrition applications and possible future pharmaceutical development.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">31969582</PMID><DateRevised><Year>2020</Year><Month>07</Month><Day>08</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2045-2322</ISSN><JournalIssue CitedMedium="Internet"><Volume>10</Volume><Issue>1</Issue><PubDate><Year>2020</Year><Month>01</Month><Day>22</Day></PubDate></JournalIssue><Title>Scientific reports</Title><ISOAbbreviation>Sci Rep</ISOAbbreviation></Journal><ArticleTitle>Plant based production of myoglobin - a novel source of the muscle heme-protein.</ArticleTitle><Pagination><MedlinePgn>920</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1038/s41598-020-57565-y</ELocationID><Abstract><AbstractText>Myoglobin is a heme-protein in the muscle of vertebrates with important functions in the oxygenation of tissues and as a regulator in nitric oxide signaling. Myoglobin from many species is also an important nutritional source of bioavailable iron. In this study, we have successfully produced human myoglobin in the leaves of Nicotiana benthamiana by transient expression using a viral vector delivered by Agrobacterium tumefaciens. Analyses confirmed that heme was incorporated and the protein was functional, with observed properties consistent with those of native myoglobins. A relatively high degree of purity could be achieved with low cost methods. The results show the high potential of plants as a production platform for heme proteins, a group of proteins of interest for iron nutrition applications and possible future pharmaceutical development.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Carlsson</LastName><ForeName>Magnus L R</ForeName><Initials>MLR</Initials><AffiliationInfo><Affiliation>Department of Plant Breeding, Swedish University of Agricultural Sciences, Box 101, 230 53, Alnarp, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kanagarajan</LastName><ForeName>Selvaraju</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Plant Breeding, Swedish University of Agricultural Sciences, Box 101, 230 53, Alnarp, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bülow</LastName><ForeName>Leif</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Division of Pure and Applied Biochemistry, Department of Chemistry, Lund University, Box 124, 221 00, Lund, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhu</LastName><ForeName>Li-Hua</ForeName><Initials>LH</Initials><AffiliationInfo><Affiliation>Department of Plant Breeding, Swedish University of Agricultural Sciences, Box 101, 230 53, Alnarp, Sweden. 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IdType="pubmed">25615702</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1987 Nov;84(21):7503-7</Citation><ArticleIdList><ArticleId IdType="pubmed">3118370</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 1993 Apr 5;268(10):6995-7010</Citation><ArticleIdList><ArticleId IdType="pubmed">8463233</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Appl Biochem. 2011 Jan-Feb;58(1):58-67</Citation><ArticleIdList><ArticleId IdType="pubmed">21446960</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Biol. 2010 Aug 15;213(Pt 16):2713-25</Citation><ArticleIdList><ArticleId IdType="pubmed">20675540</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2019 Jan 8;47(D1):D506-D515</Citation><ArticleIdList><ArticleId IdType="pubmed">30395287</ArticleId></ArticleIdList></Reference><Reference><Citation>Meat Sci. 1993;33(3):401-7</Citation><ArticleIdList><ArticleId IdType="pubmed">22060157</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 1992 Mar 19;356(6366):258-60</Citation><ArticleIdList><ArticleId IdType="pubmed">1552945</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Protoc. 2006;1(6):2876-90</Citation><ArticleIdList><ArticleId IdType="pubmed">17406547</ArticleId></ArticleIdList></Reference><Reference><Citation>Blood. 2014 Jan 30;123(5):615-24</Citation><ArticleIdList><ArticleId IdType="pubmed">24297872</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Chem. 2007 Aug 15;79(16):6236-48</Citation><ArticleIdList><ArticleId IdType="pubmed">17637042</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Biol. 2010 Aug 15;213(Pt 16):2734-40</Citation><ArticleIdList><ArticleId IdType="pubmed">20675542</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 1966 Jan 25;241(2):432-42</Citation><ArticleIdList><ArticleId IdType="pubmed">5903736</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Biol. 2003 Jun;206(Pt 12):2011-20</Citation><ArticleIdList><ArticleId IdType="pubmed">12756283</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochemistry. 1991 Feb 5;30(5):1227-37</Citation><ArticleIdList><ArticleId IdType="pubmed">1991102</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2007 Dec;145(4):1232-40</Citation><ArticleIdList><ArticleId IdType="pubmed">17720752</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Invest. 1970 Dec;49(12):2369-76</Citation><ArticleIdList><ArticleId IdType="pubmed">5480860</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Biotechnol J. 2010 Jun;8(5):620-37</Citation><ArticleIdList><ArticleId IdType="pubmed">20233333</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem J. 2016 Oct 1;473(19):3371-83</Citation><ArticleIdList><ArticleId IdType="pubmed">27470146</ArticleId></ArticleIdList></Reference><Reference><Citation>Biosci Rep. 2018 Jul 2;38(4):</Citation><ArticleIdList><ArticleId IdType="pubmed">29802155</ArticleId></ArticleIdList></Reference><Reference><Citation>Am J Clin Nutr. 2010 May;91(5):1461S-1467S</Citation><ArticleIdList><ArticleId IdType="pubmed">20200263</ArticleId></ArticleIdList></Reference><Reference><Citation>Food Nutr Sci. 2012 Jul 1;3(7):905-913</Citation><ArticleIdList><ArticleId IdType="pubmed">23459329</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 2005 Jun;23(6):718-23</Citation><ArticleIdList><ArticleId IdType="pubmed">15883585</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 2007 Feb;63(3):393-404</Citation><ArticleIdList><ArticleId IdType="pubmed">17221361</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 1960 Feb 13;185(4711):422-7</Citation><ArticleIdList><ArticleId IdType="pubmed">18990802</ArticleId></ArticleIdList></Reference><Reference><Citation>J Am Diet Assoc. 2001 Mar;101(3):294-301</Citation><ArticleIdList><ArticleId IdType="pubmed">11269606</ArticleId></ArticleIdList></Reference><Reference><Citation>Int J Environ Res Public Health. 2019 Apr 05;16(7):</Citation><ArticleIdList><ArticleId IdType="pubmed">30959755</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 1997 Mar 6;386(6620):29-30</Citation><ArticleIdList><ArticleId IdType="pubmed">9052777</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Biotechnol J. 2015 Jun;13(5):708-16</Citation><ArticleIdList><ArticleId IdType="pubmed">25470212</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Rev Drug Discov. 2004 Feb;3(2):152-9</Citation><ArticleIdList><ArticleId IdType="pubmed">15043006</ArticleId></ArticleIdList></Reference><Reference><Citation>Glob Food Sec. 2017 Mar;12:49-58</Citation><ArticleIdList><ArticleId IdType="pubmed">28580239</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochim Biophys Acta. 2008 Oct;1784(10):1471-9</Citation><ArticleIdList><ArticleId IdType="pubmed">18489914</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Nutr. 2018 Feb 14;5:12</Citation><ArticleIdList><ArticleId IdType="pubmed">29492405</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Lett. 2014 Dec;36(12):2367-79</Citation><ArticleIdList><ArticleId IdType="pubmed">25048244</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Biotechnol J. 2015 Oct;13(8):1121-35</Citation><ArticleIdList><ArticleId IdType="pubmed">26073158</ArticleId></ArticleIdList></Reference><Reference><Citation>Acta Chem Scand. 1948;2(3):264-89</Citation><ArticleIdList><ArticleId IdType="pubmed">18098533</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioconjug Chem. 2018 May 16;29(5):1560-1575</Citation><ArticleIdList><ArticleId 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