AgrobacTransV1, Main, Corpus, bibRecord, 000008

Bioactive diterpenoid metabolism and cytotoxic activities of genetically transformed Euphorbia lathyris roots.

Identifieur interne : 000008 ( Main/Corpus ); précédent : 000007; suivant : 000009

Bioactive diterpenoid metabolism and cytotoxic activities of genetically transformed Euphorbia lathyris roots.

Auteurs : Vincent A. Ricigliano ; Vincent P. Sica ; Sonja L. Knowles ; Nicole Diette ; Dianella G. Howarth ; Nicholas H. Oberlies

Source :

Phytochemistry [ 1873-3700 ] ; 2020.

RBID : pubmed:32980713

English descriptors

KwdEn :
- Diterpenes (pharmacology), Euphorbia (MeSH), Humans (MeSH), Plant Roots (MeSH).
MESH :
- chemical , pharmacology : Diterpenes.
- Euphorbia, Humans, Plant Roots.

Abstract

Plants in the genus Euphorbia produce a wide variety of pharmacologically active diterpenoids with anticancer, multidrug resistance reversal, and antiviral properties. Some are the primary industrial source of ingenol mebutate, which is approved for treatment of the precancerous skin condition actinic keratosis. Similar to other high value phytochemicals, Euphorbia diterpenoids accumulate at low concentrations in planta and chemical synthesis produces similarly low yields. We established genetically transformed root cultures of Euphorbia lathryis as a strategy to gain greater access to diterpenoids from this genus. Transformed roots produced via stem explant infection with Agrobacterium rhizogenes strain 15834 recapitulated the metabolite profiles of field-grown plant roots and aerial tissues. Several putative diterpenoids were present in transformed roots, including ingenol and closely related structures, indicating that root cultures are a promising approach to Euphorbia-specific diterpenoid production. Treatment with methyl jasmonate led to a significant, albeit transient increase in mRNA levels of early diterpenoid biosynthetic enzymes (farnesyl pyrophosphate synthase, geranylgeranyl pyrophosphate synthase, and casbene synthase), suggesting that elicitation could prove useful in future pathway characterization and metabolic engineering efforts. We also show the potential of transformed E. lathyris root cultures for natural product drug discovery applications by measuring their cytotoxic activities using a panel of human carcinoma cell lines derived from prostate, cervix, breast, and lung.

DOI: 10.1016/j.phytochem.2020.112504
PubMed: 32980713

Links to Exploration step

pubmed:32980713

Le document en format XML

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<author><name sortKey="Ricigliano, Vincent A" sort="Ricigliano, Vincent A" uniqKey="Ricigliano V" first="Vincent A" last="Ricigliano">Vincent A. Ricigliano</name>
<affiliation><nlm:affiliation>USDA-ARS, Honey Bee Breeding, Genetics and Physiology Research, Baton Rouge, LA, 70820, USA. Electronic address: Vincent.ricigliano@usda.gov.</nlm:affiliation>
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<author><name sortKey="Sica, Vincent P" sort="Sica, Vincent P" uniqKey="Sica V" first="Vincent P" last="Sica">Vincent P. Sica</name>
<affiliation><nlm:affiliation>Department of Chemistry and Biochemistry, University of North Carolina at Greensboro, Greensboro, NC, 27402, USA.</nlm:affiliation>
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<author><name sortKey="Knowles, Sonja L" sort="Knowles, Sonja L" uniqKey="Knowles S" first="Sonja L" last="Knowles">Sonja L. Knowles</name>
<affiliation><nlm:affiliation>Department of Chemistry and Biochemistry, University of North Carolina at Greensboro, Greensboro, NC, 27402, USA.</nlm:affiliation>
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<author><name sortKey="Diette, Nicole" sort="Diette, Nicole" uniqKey="Diette N" first="Nicole" last="Diette">Nicole Diette</name>
<affiliation><nlm:affiliation>Department of Dermatology, University of Colorado School of Medicine, Anschutz Medical Campus, Aurora, CO, 80227, USA; Charles C. Gates Center for Regenerative Medicine, Aurora, CO, 80227, USA.</nlm:affiliation>
</affiliation>
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<author><name sortKey="Howarth, Dianella G" sort="Howarth, Dianella G" uniqKey="Howarth D" first="Dianella G" last="Howarth">Dianella G. Howarth</name>
<affiliation><nlm:affiliation>Department of Biological Sciences, St. John's University, Jamaica, NY, 11439, USA.</nlm:affiliation>
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<author><name sortKey="Oberlies, Nicholas H" sort="Oberlies, Nicholas H" uniqKey="Oberlies N" first="Nicholas H" last="Oberlies">Nicholas H. Oberlies</name>
<affiliation><nlm:affiliation>Department of Chemistry and Biochemistry, University of North Carolina at Greensboro, Greensboro, NC, 27402, USA.</nlm:affiliation>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Bioactive diterpenoid metabolism and cytotoxic activities of genetically transformed Euphorbia lathyris roots.</title>
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<author><name sortKey="Sica, Vincent P" sort="Sica, Vincent P" uniqKey="Sica V" first="Vincent P" last="Sica">Vincent P. Sica</name>
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<author><name sortKey="Knowles, Sonja L" sort="Knowles, Sonja L" uniqKey="Knowles S" first="Sonja L" last="Knowles">Sonja L. Knowles</name>
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<author><name sortKey="Diette, Nicole" sort="Diette, Nicole" uniqKey="Diette N" first="Nicole" last="Diette">Nicole Diette</name>
<affiliation><nlm:affiliation>Department of Dermatology, University of Colorado School of Medicine, Anschutz Medical Campus, Aurora, CO, 80227, USA; Charles C. Gates Center for Regenerative Medicine, Aurora, CO, 80227, USA.</nlm:affiliation>
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<author><name sortKey="Howarth, Dianella G" sort="Howarth, Dianella G" uniqKey="Howarth D" first="Dianella G" last="Howarth">Dianella G. Howarth</name>
<affiliation><nlm:affiliation>Department of Biological Sciences, St. John's University, Jamaica, NY, 11439, USA.</nlm:affiliation>
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<author><name sortKey="Oberlies, Nicholas H" sort="Oberlies, Nicholas H" uniqKey="Oberlies N" first="Nicholas H" last="Oberlies">Nicholas H. Oberlies</name>
<affiliation><nlm:affiliation>Department of Chemistry and Biochemistry, University of North Carolina at Greensboro, Greensboro, NC, 27402, USA.</nlm:affiliation>
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<series><title level="j">Phytochemistry</title>
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<front><div type="abstract" xml:lang="en">Plants in the genus Euphorbia produce a wide variety of pharmacologically active diterpenoids with anticancer, multidrug resistance reversal, and antiviral properties. Some are the primary industrial source of ingenol mebutate, which is approved for treatment of the precancerous skin condition actinic keratosis. Similar to other high value phytochemicals, Euphorbia diterpenoids accumulate at low concentrations in planta and chemical synthesis produces similarly low yields. We established genetically transformed root cultures of Euphorbia lathryis as a strategy to gain greater access to diterpenoids from this genus. Transformed roots produced via stem explant infection with Agrobacterium rhizogenes strain 15834 recapitulated the metabolite profiles of field-grown plant roots and aerial tissues. Several putative diterpenoids were present in transformed roots, including ingenol and closely related structures, indicating that root cultures are a promising approach to Euphorbia-specific diterpenoid production. Treatment with methyl jasmonate led to a significant, albeit transient increase in mRNA levels of early diterpenoid biosynthetic enzymes (farnesyl pyrophosphate synthase, geranylgeranyl pyrophosphate synthase, and casbene synthase), suggesting that elicitation could prove useful in future pathway characterization and metabolic engineering efforts. We also show the potential of transformed E. lathyris root cultures for natural product drug discovery applications by measuring their cytotoxic activities using a panel of human carcinoma cell lines derived from prostate, cervix, breast, and lung.</div>
</front>
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<Title>Phytochemistry</Title>
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<Abstract><AbstractText>Plants in the genus Euphorbia produce a wide variety of pharmacologically active diterpenoids with anticancer, multidrug resistance reversal, and antiviral properties. Some are the primary industrial source of ingenol mebutate, which is approved for treatment of the precancerous skin condition actinic keratosis. Similar to other high value phytochemicals, Euphorbia diterpenoids accumulate at low concentrations in planta and chemical synthesis produces similarly low yields. We established genetically transformed root cultures of Euphorbia lathryis as a strategy to gain greater access to diterpenoids from this genus. Transformed roots produced via stem explant infection with Agrobacterium rhizogenes strain 15834 recapitulated the metabolite profiles of field-grown plant roots and aerial tissues. Several putative diterpenoids were present in transformed roots, including ingenol and closely related structures, indicating that root cultures are a promising approach to Euphorbia-specific diterpenoid production. Treatment with methyl jasmonate led to a significant, albeit transient increase in mRNA levels of early diterpenoid biosynthetic enzymes (farnesyl pyrophosphate synthase, geranylgeranyl pyrophosphate synthase, and casbene synthase), suggesting that elicitation could prove useful in future pathway characterization and metabolic engineering efforts. We also show the potential of transformed E. lathyris root cultures for natural product drug discovery applications by measuring their cytotoxic activities using a panel of human carcinoma cell lines derived from prostate, cervix, breast, and lung.</AbstractText>
<CopyrightInformation>Published by Elsevier Ltd.</CopyrightInformation>
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<ForeName>Vincent A</ForeName>
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<AffiliationInfo><Affiliation>Department of Chemistry and Biochemistry, University of North Carolina at Greensboro, Greensboro, NC, 27402, USA.</Affiliation>
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<Keyword MajorTopicYN="N">Diterpene</Keyword>
<Keyword MajorTopicYN="N">Euphorbia</Keyword>
<Keyword MajorTopicYN="N">Ingenol</Keyword>
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<Month>07</Month>
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Bioactive diterpenoid metabolism and cytotoxic activities of genetically transformed Euphorbia lathyris roots.

Bioactive diterpenoid metabolism and cytotoxic activities of genetically transformed Euphorbia lathyris roots.

Source :

English descriptors

Abstract

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki