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Splenic retention of Plasmodium falciparum gametocytes to block the transmission of malaria.

Identifieur interne : 002B48 ( PubMed/Curation ); précédent : 002B47; suivant : 002B49

Splenic retention of Plasmodium falciparum gametocytes to block the transmission of malaria.

Auteurs : Julien Duez [France] ; John P. Holleran [Australie] ; Papa Alioune Ndour [France] ; Sasdekumar Loganathan [Australie] ; Pascal Amireault [France] ; Olivier Français [France] ; Wassim El Nemer [France] ; Bruno Le Pioufle [France] ; Inês F. Amado [France] ; Sylvie Garcia [France] ; Nathalie Chartrel [France] ; Caroline Le Van Kim [France] ; Catherine Lavazec [France] ; Vicky M. Avery [Australie] ; Pierre A. Buffet [Oman]

Source :

Antimicrobial agents and chemotherapy [ 1098-6596 ] ; 2015.

RBID : pubmed:25941228

Descripteurs français

KwdFr :
- Animaux, Antienzymes (pharmacologie), Antipaludiques (pharmacologie), Automatisation, Cytométrie en flux, Filtration, Macrophages (parasitologie), Microsphères, Modèles biologiques, Numération des oeufs de parasites, Numération des érythrocytes, Oxazoles (pharmacologie), Paludisme à Plasmodium falciparum (), Paludisme à Plasmodium falciparum (parasitologie), Paludisme à Plasmodium falciparum (transmission), Plasmodium falciparum (croissance et développement), Rate (), Rate (parasitologie), Souris, Techniques d'analyse microfluidique, Traitement d'image par ordinateur, Érythrocytes (parasitologie).
MESH :
- croissance et développement : Plasmodium falciparum.
- parasitologie : Macrophages, Paludisme à Plasmodium falciparum, Rate, Érythrocytes.
- pharmacologie : Antienzymes, Antipaludiques, Oxazoles.
- Animaux, Automatisation, Cytométrie en flux, Filtration, Microsphères, Modèles biologiques, Numération des oeufs de parasites, Numération des érythrocytes, Paludisme à Plasmodium falciparum, Rate, Souris, Techniques d'analyse microfluidique, Traitement d'image par ordinateur.

English descriptors

KwdEn :
- Animals, Antimalarials (pharmacology), Automation, Enzyme Inhibitors (pharmacology), Erythrocyte Count, Erythrocytes (parasitology), Filtration, Flow Cytometry, Image Processing, Computer-Assisted, Macrophages (parasitology), Malaria, Falciparum (parasitology), Malaria, Falciparum (prevention & control), Malaria, Falciparum (transmission), Mice, Microfluidic Analytical Techniques, Microspheres, Models, Biological, Oxazoles (pharmacology), Parasite Egg Count, Plasmodium falciparum (growth & development), Spleen (drug effects), Spleen (parasitology).
MESH :
- chemical , pharmacology : Antimalarials, Enzyme Inhibitors, Oxazoles.
- drug effects : Spleen.
- growth & development : Plasmodium falciparum.
- parasitology : Erythrocytes, Macrophages, Malaria, Falciparum, Spleen.
- prevention & control : Malaria, Falciparum.
- transmission : Malaria, Falciparum.
- Animals, Automation, Erythrocyte Count, Filtration, Flow Cytometry, Image Processing, Computer-Assisted, Mice, Microfluidic Analytical Techniques, Microspheres, Models, Biological, Parasite Egg Count.

Abstract

Plasmodium falciparum is transmitted from humans to Anopheles mosquito vectors via the sexual erythrocytic forms termed gametocytes. Erythrocyte filtration through microsphere layers (microsphiltration) had shown that circulating gametocytes are deformable. Compounds reducing gametocyte deformability would induce their splenic clearance, thus removing them from the blood circulation and blocking malaria transmission. The hand-made, single-sample prototype for microsphiltration was miniaturized to a 96-well microtiter plate format, and gametocyte retention in the microsphere filters was quantified by high-content imaging. The stiffening activity of 40 pharmacological compounds was assessed in microtiter plates, using a small molecule (calyculin) as a positive control. The stiffening activity of calyculin was assessed in spleen-mimetic microfluidic chips and in macrophage-depleted mice. Marked mechanical retention (80% to 90%) of mature gametocytes was obtained in microplates following exposure to calyculin at concentrations with no effect on parasite viability. Of the 40 compounds tested, including 20 antimalarials, only 5 endoperoxides significantly increased gametocyte retention (1.5- to 2.5-fold; 24 h of exposure at 1 μM). Mature gametocytes exposed to calyculin accumulated in microfluidic chips and were cleared from the circulation of macrophage-depleted mice as rapidly as heat-stiffened erythrocytes, thus confirming results obtained using the microsphiltration assay. An automated miniaturized approach to select compounds for their gametocyte-stiffening effect has been established. Stiffening induces gametocyte clearance both in vitro and in vivo. Based on physiologically validated tools, this screening cascade can identify novel compounds and uncover new targets to block malaria transmission. Innovative applications in hematology are also envisioned.

DOI: 10.1128/AAC.05030-14
PubMed: 25941228

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<author><name sortKey="Chartrel, Nathalie" sort="Chartrel, Nathalie" uniqKey="Chartrel N" first="Nathalie" last="Chartrel">Nathalie Chartrel</name>
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<author><name sortKey="Le Van Kim, Caroline" sort="Le Van Kim, Caroline" uniqKey="Le Van Kim C" first="Caroline" last="Le Van Kim">Caroline Le Van Kim</name>
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<author><name sortKey="Lavazec, Catherine" sort="Lavazec, Catherine" uniqKey="Lavazec C" first="Catherine" last="Lavazec">Catherine Lavazec</name>
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<author><name sortKey="Avery, Vicky M" sort="Avery, Vicky M" uniqKey="Avery V" first="Vicky M" last="Avery">Vicky M. Avery</name>
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<author><name sortKey="Buffet, Pierre A" sort="Buffet, Pierre A" uniqKey="Buffet P" first="Pierre A" last="Buffet">Pierre A. Buffet</name>
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<country wicri:rule="url">Oman</country>
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<author><name sortKey="Loganathan, Sasdekumar" sort="Loganathan, Sasdekumar" uniqKey="Loganathan S" first="Sasdekumar" last="Loganathan">Sasdekumar Loganathan</name>
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<author><name sortKey="Amireault, Pascal" sort="Amireault, Pascal" uniqKey="Amireault P" first="Pascal" last="Amireault">Pascal Amireault</name>
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<country xml:lang="fr">France</country>
<wicri:regionArea>INSERM U1163/CNRS ERL 8254, Institut Imagine, Paris Descartes-Université Sorbonne Paris Cité, Paris, France Laboratoire d'Excellence GR-Ex, Paris, France Institut National de la Transfusion Sanguine (INTS), Paris</wicri:regionArea>
</affiliation>
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<author><name sortKey="Francais, Olivier" sort="Francais, Olivier" uniqKey="Francais O" first="Olivier" last="Français">Olivier Français</name>
<affiliation wicri:level="1"><nlm:affiliation>Ecole Normale Supérieure de Cachan, CNRS, BIOMIS-SATIE, UMR 8029, Cachan, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Ecole Normale Supérieure de Cachan, CNRS, BIOMIS-SATIE, UMR 8029, Cachan</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="El Nemer, Wassim" sort="El Nemer, Wassim" uniqKey="El Nemer W" first="Wassim" last="El Nemer">Wassim El Nemer</name>
<affiliation wicri:level="1"><nlm:affiliation>Institut National de la Transfusion Sanguine (INTS), Paris, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Institut National de la Transfusion Sanguine (INTS), Paris</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Le Pioufle, Bruno" sort="Le Pioufle, Bruno" uniqKey="Le Pioufle B" first="Bruno" last="Le Pioufle">Bruno Le Pioufle</name>
<affiliation wicri:level="1"><nlm:affiliation>Ecole Normale Supérieure de Cachan, CNRS, BIOMIS-SATIE, UMR 8029, Cachan, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Ecole Normale Supérieure de Cachan, CNRS, BIOMIS-SATIE, UMR 8029, Cachan</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Amado, Ines F" sort="Amado, Ines F" uniqKey="Amado I" first="Inês F" last="Amado">Inês F. Amado</name>
<affiliation wicri:level="1"><nlm:affiliation>Institut Pasteur de Paris, Paris, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Institut Pasteur de Paris, Paris</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Garcia, Sylvie" sort="Garcia, Sylvie" uniqKey="Garcia S" first="Sylvie" last="Garcia">Sylvie Garcia</name>
<affiliation wicri:level="1"><nlm:affiliation>Institut Pasteur de Paris, Paris, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Institut Pasteur de Paris, Paris</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Chartrel, Nathalie" sort="Chartrel, Nathalie" uniqKey="Chartrel N" first="Nathalie" last="Chartrel">Nathalie Chartrel</name>
<affiliation wicri:level="1"><nlm:affiliation>Centre d'Immunologie et des Maladies Infectieuses de Paris (CIMI-Paris), INSERM U1135, UPMC CR7, CNRS ERL 8255, Paris, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Centre d'Immunologie et des Maladies Infectieuses de Paris (CIMI-Paris), INSERM U1135, UPMC CR7, CNRS ERL 8255, Paris</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Le Van Kim, Caroline" sort="Le Van Kim, Caroline" uniqKey="Le Van Kim C" first="Caroline" last="Le Van Kim">Caroline Le Van Kim</name>
<affiliation wicri:level="1"><nlm:affiliation>Institut National de la Transfusion Sanguine (INTS), Paris, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Institut National de la Transfusion Sanguine (INTS), Paris</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Lavazec, Catherine" sort="Lavazec, Catherine" uniqKey="Lavazec C" first="Catherine" last="Lavazec">Catherine Lavazec</name>
<affiliation wicri:level="1"><nlm:affiliation>Institut Pasteur de Paris, Paris, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Institut Pasteur de Paris, Paris</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Avery, Vicky M" sort="Avery, Vicky M" uniqKey="Avery V" first="Vicky M" last="Avery">Vicky M. Avery</name>
<affiliation wicri:level="1"><nlm:affiliation>Eskitis Institute for Drug Discovery, Griffith University, Nathan, QLD, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Eskitis Institute for Drug Discovery, Griffith University, Nathan, QLD</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Buffet, Pierre A" sort="Buffet, Pierre A" uniqKey="Buffet P" first="Pierre A" last="Buffet">Pierre A. Buffet</name>
<affiliation wicri:level="1"><nlm:affiliation>Centre d'Immunologie et des Maladies Infectieuses de Paris (CIMI-Paris), INSERM U1135, UPMC CR7, CNRS ERL 8255, Paris, France Laboratoire d'Excellence GR-Ex, Paris, France pabuffet@gmail.com.</nlm:affiliation>
<country wicri:rule="url">Oman</country>
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</affiliation>
</author>
</analytic>
<series><title level="j">Antimicrobial agents and chemotherapy</title>
<idno type="eISSN">1098-6596</idno>
<imprint><date when="2015" type="published">2015</date>
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<term>Antimalarials (pharmacology)</term>
<term>Automation</term>
<term>Enzyme Inhibitors (pharmacology)</term>
<term>Erythrocyte Count</term>
<term>Erythrocytes (parasitology)</term>
<term>Filtration</term>
<term>Flow Cytometry</term>
<term>Image Processing, Computer-Assisted</term>
<term>Macrophages (parasitology)</term>
<term>Malaria, Falciparum (parasitology)</term>
<term>Malaria, Falciparum (prevention & control)</term>
<term>Malaria, Falciparum (transmission)</term>
<term>Mice</term>
<term>Microfluidic Analytical Techniques</term>
<term>Microspheres</term>
<term>Models, Biological</term>
<term>Oxazoles (pharmacology)</term>
<term>Parasite Egg Count</term>
<term>Plasmodium falciparum (growth & development)</term>
<term>Spleen (drug effects)</term>
<term>Spleen (parasitology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Antienzymes (pharmacologie)</term>
<term>Antipaludiques (pharmacologie)</term>
<term>Automatisation</term>
<term>Cytométrie en flux</term>
<term>Filtration</term>
<term>Macrophages (parasitologie)</term>
<term>Microsphères</term>
<term>Modèles biologiques</term>
<term>Numération des oeufs de parasites</term>
<term>Numération des érythrocytes</term>
<term>Oxazoles (pharmacologie)</term>
<term>Paludisme à Plasmodium falciparum ()</term>
<term>Paludisme à Plasmodium falciparum (parasitologie)</term>
<term>Paludisme à Plasmodium falciparum (transmission)</term>
<term>Plasmodium falciparum (croissance et développement)</term>
<term>Rate ()</term>
<term>Rate (parasitologie)</term>
<term>Souris</term>
<term>Techniques d'analyse microfluidique</term>
<term>Traitement d'image par ordinateur</term>
<term>Érythrocytes (parasitologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Antimalarials</term>
<term>Enzyme Inhibitors</term>
<term>Oxazoles</term>
</keywords>
<keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Plasmodium falciparum</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Spleen</term>
</keywords>
<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Plasmodium falciparum</term>
</keywords>
<keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Macrophages</term>
<term>Paludisme à Plasmodium falciparum</term>
<term>Rate</term>
<term>Érythrocytes</term>
</keywords>
<keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Erythrocytes</term>
<term>Macrophages</term>
<term>Malaria, Falciparum</term>
<term>Spleen</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antienzymes</term>
<term>Antipaludiques</term>
<term>Oxazoles</term>
</keywords>
<keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Malaria, Falciparum</term>
</keywords>
<keywords scheme="MESH" qualifier="transmission" xml:lang="en"><term>Malaria, Falciparum</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Automation</term>
<term>Erythrocyte Count</term>
<term>Filtration</term>
<term>Flow Cytometry</term>
<term>Image Processing, Computer-Assisted</term>
<term>Mice</term>
<term>Microfluidic Analytical Techniques</term>
<term>Microspheres</term>
<term>Models, Biological</term>
<term>Parasite Egg Count</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Automatisation</term>
<term>Cytométrie en flux</term>
<term>Filtration</term>
<term>Microsphères</term>
<term>Modèles biologiques</term>
<term>Numération des oeufs de parasites</term>
<term>Numération des érythrocytes</term>
<term>Paludisme à Plasmodium falciparum</term>
<term>Rate</term>
<term>Souris</term>
<term>Techniques d'analyse microfluidique</term>
<term>Traitement d'image par ordinateur</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Plasmodium falciparum is transmitted from humans to Anopheles mosquito vectors via the sexual erythrocytic forms termed gametocytes. Erythrocyte filtration through microsphere layers (microsphiltration) had shown that circulating gametocytes are deformable. Compounds reducing gametocyte deformability would induce their splenic clearance, thus removing them from the blood circulation and blocking malaria transmission. The hand-made, single-sample prototype for microsphiltration was miniaturized to a 96-well microtiter plate format, and gametocyte retention in the microsphere filters was quantified by high-content imaging. The stiffening activity of 40 pharmacological compounds was assessed in microtiter plates, using a small molecule (calyculin) as a positive control. The stiffening activity of calyculin was assessed in spleen-mimetic microfluidic chips and in macrophage-depleted mice. Marked mechanical retention (80% to 90%) of mature gametocytes was obtained in microplates following exposure to calyculin at concentrations with no effect on parasite viability. Of the 40 compounds tested, including 20 antimalarials, only 5 endoperoxides significantly increased gametocyte retention (1.5- to 2.5-fold; 24 h of exposure at 1 μM). Mature gametocytes exposed to calyculin accumulated in microfluidic chips and were cleared from the circulation of macrophage-depleted mice as rapidly as heat-stiffened erythrocytes, thus confirming results obtained using the microsphiltration assay. An automated miniaturized approach to select compounds for their gametocyte-stiffening effect has been established. Stiffening induces gametocyte clearance both in vitro and in vivo. Based on physiologically validated tools, this screening cascade can identify novel compounds and uncover new targets to block malaria transmission. Innovative applications in hematology are also envisioned.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25941228</PMID>
<DateCreated><Year>2015</Year>
<Month>06</Month>
<Day>13</Day>
</DateCreated>
<DateCompleted><Year>2016</Year>
<Month>03</Month>
<Day>15</Day>
</DateCompleted>
<DateRevised><Year>2016</Year>
<Month>11</Month>
<Day>25</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1098-6596</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>59</Volume>
<Issue>7</Issue>
<PubDate><Year>2015</Year>
<Month>Jul</Month>
</PubDate>
</JournalIssue>
<Title>Antimicrobial agents and chemotherapy</Title>
<ISOAbbreviation>Antimicrob. Agents Chemother.</ISOAbbreviation>
</Journal>
<ArticleTitle>Splenic retention of Plasmodium falciparum gametocytes to block the transmission of malaria.</ArticleTitle>
<Pagination><MedlinePgn>4206-14</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1128/AAC.05030-14</ELocationID>
<Abstract><AbstractText>Plasmodium falciparum is transmitted from humans to Anopheles mosquito vectors via the sexual erythrocytic forms termed gametocytes. Erythrocyte filtration through microsphere layers (microsphiltration) had shown that circulating gametocytes are deformable. Compounds reducing gametocyte deformability would induce their splenic clearance, thus removing them from the blood circulation and blocking malaria transmission. The hand-made, single-sample prototype for microsphiltration was miniaturized to a 96-well microtiter plate format, and gametocyte retention in the microsphere filters was quantified by high-content imaging. The stiffening activity of 40 pharmacological compounds was assessed in microtiter plates, using a small molecule (calyculin) as a positive control. The stiffening activity of calyculin was assessed in spleen-mimetic microfluidic chips and in macrophage-depleted mice. Marked mechanical retention (80% to 90%) of mature gametocytes was obtained in microplates following exposure to calyculin at concentrations with no effect on parasite viability. Of the 40 compounds tested, including 20 antimalarials, only 5 endoperoxides significantly increased gametocyte retention (1.5- to 2.5-fold; 24 h of exposure at 1 μM). Mature gametocytes exposed to calyculin accumulated in microfluidic chips and were cleared from the circulation of macrophage-depleted mice as rapidly as heat-stiffened erythrocytes, thus confirming results obtained using the microsphiltration assay. An automated miniaturized approach to select compounds for their gametocyte-stiffening effect has been established. Stiffening induces gametocyte clearance both in vitro and in vivo. Based on physiologically validated tools, this screening cascade can identify novel compounds and uncover new targets to block malaria transmission. Innovative applications in hematology are also envisioned.</AbstractText>
<CopyrightInformation>Copyright © 2015, American Society for Microbiology. All Rights Reserved.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Duez</LastName>
<ForeName>Julien</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>Centre d'Immunologie et des Maladies Infectieuses de Paris (CIMI-Paris), INSERM U1135, UPMC CR7, CNRS ERL 8255, Paris, France Eskitis Institute for Drug Discovery, Griffith University, Nathan, QLD, Australia Laboratoire d'Excellence GR-Ex, Paris, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Holleran</LastName>
<ForeName>John P</ForeName>
<Initials>JP</Initials>
<AffiliationInfo><Affiliation>Eskitis Institute for Drug Discovery, Griffith University, Nathan, QLD, Australia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ndour</LastName>
<ForeName>Papa Alioune</ForeName>
<Initials>PA</Initials>
<AffiliationInfo><Affiliation>Centre d'Immunologie et des Maladies Infectieuses de Paris (CIMI-Paris), INSERM U1135, UPMC CR7, CNRS ERL 8255, Paris, France Laboratoire d'Excellence GR-Ex, Paris, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Loganathan</LastName>
<ForeName>Sasdekumar</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Eskitis Institute for Drug Discovery, Griffith University, Nathan, QLD, Australia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Amireault</LastName>
<ForeName>Pascal</ForeName>
<Initials>P</Initials>
<AffiliationInfo><Affiliation>INSERM U1163/CNRS ERL 8254, Institut Imagine, Paris Descartes-Université Sorbonne Paris Cité, Paris, France Laboratoire d'Excellence GR-Ex, Paris, France Institut National de la Transfusion Sanguine (INTS), Paris, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Français</LastName>
<ForeName>Olivier</ForeName>
<Initials>O</Initials>
<AffiliationInfo><Affiliation>Ecole Normale Supérieure de Cachan, CNRS, BIOMIS-SATIE, UMR 8029, Cachan, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>El Nemer</LastName>
<ForeName>Wassim</ForeName>
<Initials>W</Initials>
<AffiliationInfo><Affiliation>Institut National de la Transfusion Sanguine (INTS), Paris, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Le Pioufle</LastName>
<ForeName>Bruno</ForeName>
<Initials>B</Initials>
<AffiliationInfo><Affiliation>Ecole Normale Supérieure de Cachan, CNRS, BIOMIS-SATIE, UMR 8029, Cachan, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Amado</LastName>
<ForeName>Inês F</ForeName>
<Initials>IF</Initials>
<AffiliationInfo><Affiliation>Institut Pasteur de Paris, Paris, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Garcia</LastName>
<ForeName>Sylvie</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Institut Pasteur de Paris, Paris, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Chartrel</LastName>
<ForeName>Nathalie</ForeName>
<Initials>N</Initials>
<AffiliationInfo><Affiliation>Centre d'Immunologie et des Maladies Infectieuses de Paris (CIMI-Paris), INSERM U1135, UPMC CR7, CNRS ERL 8255, Paris, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Le Van Kim</LastName>
<ForeName>Caroline</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>Institut National de la Transfusion Sanguine (INTS), Paris, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Lavazec</LastName>
<ForeName>Catherine</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>Institut Pasteur de Paris, Paris, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Avery</LastName>
<ForeName>Vicky M</ForeName>
<Initials>VM</Initials>
<AffiliationInfo><Affiliation>Eskitis Institute for Drug Discovery, Griffith University, Nathan, QLD, Australia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Buffet</LastName>
<ForeName>Pierre A</ForeName>
<Initials>PA</Initials>
<AffiliationInfo><Affiliation>Centre d'Immunologie et des Maladies Infectieuses de Paris (CIMI-Paris), INSERM U1135, UPMC CR7, CNRS ERL 8255, Paris, France Laboratoire d'Excellence GR-Ex, Paris, France pabuffet@gmail.com.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2015</Year>
<Month>05</Month>
<Day>04</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>Antimicrob Agents Chemother</MedlineTA>
<NlmUniqueID>0315061</NlmUniqueID>
<ISSNLinking>0066-4804</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D000962">Antimalarials</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D004791">Enzyme Inhibitors</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D010080">Oxazoles</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>7D07U14TK3</RegistryNumber>
<NameOfSubstance UI="C059041">calyculin A</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
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<MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000962" MajorTopicYN="N">Antimalarials</DescriptorName>
<QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001331" MajorTopicYN="N">Automation</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004791" MajorTopicYN="N">Enzyme Inhibitors</DescriptorName>
<QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004906" MajorTopicYN="N">Erythrocyte Count</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004912" MajorTopicYN="N">Erythrocytes</DescriptorName>
<QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005374" MajorTopicYN="N">Filtration</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005434" MajorTopicYN="N">Flow Cytometry</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007091" MajorTopicYN="N">Image Processing, Computer-Assisted</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008264" MajorTopicYN="N">Macrophages</DescriptorName>
<QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016778" MajorTopicYN="N">Malaria, Falciparum</DescriptorName>
<QualifierName UI="Q000469" MajorTopicYN="Y">parasitology</QualifierName>
<QualifierName UI="Q000517" MajorTopicYN="N">prevention & control</QualifierName>
<QualifierName UI="Q000635" MajorTopicYN="Y">transmission</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D046210" MajorTopicYN="N">Microfluidic Analytical Techniques</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008863" MajorTopicYN="N">Microspheres</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008954" MajorTopicYN="N">Models, Biological</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010080" MajorTopicYN="N">Oxazoles</DescriptorName>
<QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010270" MajorTopicYN="N">Parasite Egg Count</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010963" MajorTopicYN="N">Plasmodium falciparum</DescriptorName>
<QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013154" MajorTopicYN="N">Spleen</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
<QualifierName UI="Q000469" MajorTopicYN="Y">parasitology</QualifierName>
</MeshHeading>
</MeshHeadingList>
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HfdSelect -h $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd -nk 002B48 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Asie
   |area=    AustralieFrV1
   |flux=    PubMed
   |étape=   Curation
   |type=    RBID
   |clé=     pubmed:25941228
   |texte=   Splenic retention of Plasmodium falciparum gametocytes to block the transmission of malaria.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Curation/RBID.i   -Sk "pubmed:25941228" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd   \
       | NlmPubMed2Wicri -a AustralieFrV1

This area was generated with Dilib version V0.6.33.
Data generation: Tue Dec 5 10:43:12 2017. Site generation: Tue Mar 5 14:07:20 2024

	Serveur d'exploration sur les relations entre la France et l'Australie
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur les relations entre la France et l'Australie

Splenic retention of Plasmodium falciparum gametocytes to block the transmission of malaria.

Splenic retention of Plasmodium falciparum gametocytes to block the transmission of malaria.

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Pour générer des pages wiki