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Inhibitors of c-Jun phosphorylation impede ovine primordial follicle activation.

Identifieur interne : 001E58 ( PubMed/Curation ); précédent : 001E57; suivant : 001E59

Inhibitors of c-Jun phosphorylation impede ovine primordial follicle activation.

Auteurs : Michael J. Bertoldo [Australie] ; Jérémy Bernard [France] ; Nicolas Duffard [France] ; Guillaume Tsikis [France] ; Sabine Alves [France] ; Laure Calais [France] ; Svetlana Uzbekova [France] ; Danielle Monniaux [France] ; Pascal Mermillod [France] ; Yann Locatelli [France]

Source :

RBID : pubmed:26908644

Abstract

Is the c-Jun-N-terminal kinase (JNK) pathway implicated in primordial follicle activation?

DOI: 10.1093/molehr/gaw012
PubMed: 26908644

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pubmed:26908644

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<name sortKey="Uzbekova, Svetlana" sort="Uzbekova, Svetlana" uniqKey="Uzbekova S" first="Svetlana" last="Uzbekova">Svetlana Uzbekova</name>
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<name sortKey="Monniaux, Danielle" sort="Monniaux, Danielle" uniqKey="Monniaux D" first="Danielle" last="Monniaux">Danielle Monniaux</name>
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<nlm:affiliation>INRA, UMR85 Physiologie de la Reproduction et des Comportements, Nouzilly 37380, France CNRS, UMR7247, Nouzilly 37380, France Université François Rabelais de Tours, Tours 37041, France IFCE, Nouzilly 37380, France.</nlm:affiliation>
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<name sortKey="Locatelli, Yann" sort="Locatelli, Yann" uniqKey="Locatelli Y" first="Yann" last="Locatelli">Yann Locatelli</name>
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<title level="j">Molecular human reproduction</title>
<idno type="eISSN">1460-2407</idno>
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<div type="abstract" xml:lang="en">Is the c-Jun-N-terminal kinase (JNK) pathway implicated in primordial follicle activation?</div>
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<PMID Version="1">26908644</PMID>
<DateCreated>
<Year>2016</Year>
<Month>04</Month>
<Day>28</Day>
</DateCreated>
<DateRevised>
<Year>2016</Year>
<Month>04</Month>
<Day>28</Day>
</DateRevised>
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<Journal>
<ISSN IssnType="Electronic">1460-2407</ISSN>
<JournalIssue CitedMedium="Internet">
<Volume>22</Volume>
<Issue>5</Issue>
<PubDate>
<Year>2016</Year>
<Month>May</Month>
</PubDate>
</JournalIssue>
<Title>Molecular human reproduction</Title>
<ISOAbbreviation>Mol. Hum. Reprod.</ISOAbbreviation>
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<ArticleTitle>Inhibitors of c-Jun phosphorylation impede ovine primordial follicle activation.</ArticleTitle>
<Pagination>
<MedlinePgn>338-49</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1093/molehr/gaw012</ELocationID>
<Abstract>
<AbstractText Label="STUDY HYPOTHESIS" NlmCategory="OBJECTIVE">Is the c-Jun-N-terminal kinase (JNK) pathway implicated in primordial follicle activation?</AbstractText>
<AbstractText Label="STUDY FINDING" NlmCategory="UNASSIGNED">Culture of ovine ovarian cortex in the presence of two different c-Jun phosphorylation inhibitors impeded pre-antral follicle activation.</AbstractText>
<AbstractText Label="WHAT IS KNOWN ALREADY" NlmCategory="BACKGROUND">Despite its importance for fertility preservation therapies, the mechanisms of primordial follicle activation are poorly understood. Amongst different signalling pathways potentially involved, the JNK pathway has been previously shown to be essential for cell cycle progression and pre-antral follicle development in mice.</AbstractText>
<AbstractText Label="STUDY DESIGN, SAMPLES/MATERIALS, METHODS" NlmCategory="UNASSIGNED">Ovine ovarian cortex pieces were cultured with varying concentrations of SP600125, JNK inhibitor VIII or anti-Mullerian hormone (AMH) in the presence of FSH for 9 days. Follicular morphometry and immunohistochemistry for proliferating cell nuclear antigen (PCNA), apoptosis and follicle activation (Foxo3a) were assessed.</AbstractText>
<AbstractText Label="MAIN RESULTS AND THE ROLE OF CHANCE" NlmCategory="RESULTS">Inhibition of primordial follicle activation occurred in the presence of SP600125, JNK inhibitor VIII and AMH when compared with controls (all P < 0.05) after 2 days of culture. However, only in the highest concentrations used was the inhibition of activation associated with induction of follicular apoptosis (P < 0.05). In growing follicles, PCNA antigen expression was reduced when the JNK inhibitors or AMH were used (P < 0.05 versus control), indicating reduced proliferation of the somatic compartment.</AbstractText>
<AbstractText Label="LIMITATIONS, REASONS FOR CAUTION" NlmCategory="CONCLUSIONS">Although we evaluated the effects of inhibition of c-Jun phosphorylation on primordial follicle development, we did not determine the cellular targets and mechanism of action of the inhibitors.</AbstractText>
<AbstractText Label="WIDER IMPLICATIONS OF THE FINDINGS" NlmCategory="CONCLUSIONS">These results are the first to implicate the JNK pathway in primordial follicle activation and could have significant consequences for the successful development of fertility preservation strategies and our understanding of primordial follicle activation.</AbstractText>
<AbstractText Label="LARGE SCALE DATA" NlmCategory="UNASSIGNED">n/a.</AbstractText>
<AbstractText Label="STUDY FUNDING AND COMPETING INTERESTS" NlmCategory="UNASSIGNED">Dr Michael J. Bertoldo and the laboratories involved in the present study were supported by a grant from 'Région Centre' (CRYOVAIRE, Grant number #320000268). There are no conflicts of interest to declare.</AbstractText>
<CopyrightInformation>© The Author 2016. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oup.com.</CopyrightInformation>
</Abstract>
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<LastName>Bertoldo</LastName>
<ForeName>Michael J</ForeName>
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<Affiliation>INRA, UMR85 Physiologie de la Reproduction et des Comportements, Nouzilly 37380, France School of Women's and Children's Health, Discipline of Obstetrics and Gynaecology, University of New South Wales, Sydney, Australia michael.bertoldo@unsw.edu.au.</Affiliation>
</AffiliationInfo>
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<LastName>Bernard</LastName>
<ForeName>Jérémy</ForeName>
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<Affiliation>INRA, UMR85 Physiologie de la Reproduction et des Comportements, Nouzilly 37380, France MNHN, Laboratoire de la Réserve de la Haute Touche, Obterre 36290, France.</Affiliation>
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<Affiliation>INRA, UMR85 Physiologie de la Reproduction et des Comportements, Nouzilly 37380, France MNHN, Laboratoire de la Réserve de la Haute Touche, Obterre 36290, France.</Affiliation>
</AffiliationInfo>
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<LastName>Tsikis</LastName>
<ForeName>Guillaume</ForeName>
<Initials>G</Initials>
<AffiliationInfo>
<Affiliation>INRA, UMR85 Physiologie de la Reproduction et des Comportements, Nouzilly 37380, France CNRS, UMR7247, Nouzilly 37380, France Université François Rabelais de Tours, Tours 37041, France IFCE, Nouzilly 37380, France.</Affiliation>
</AffiliationInfo>
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<LastName>Alves</LastName>
<ForeName>Sabine</ForeName>
<Initials>S</Initials>
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<Affiliation>INRA, UMR85 Physiologie de la Reproduction et des Comportements, Nouzilly 37380, France CNRS, UMR7247, Nouzilly 37380, France Université François Rabelais de Tours, Tours 37041, France IFCE, Nouzilly 37380, France.</Affiliation>
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<LastName>Calais</LastName>
<ForeName>Laure</ForeName>
<Initials>L</Initials>
<AffiliationInfo>
<Affiliation>INRA, UMR85 Physiologie de la Reproduction et des Comportements, Nouzilly 37380, France.</Affiliation>
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<LastName>Uzbekova</LastName>
<ForeName>Svetlana</ForeName>
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<LastName>Monniaux</LastName>
<ForeName>Danielle</ForeName>
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<Affiliation>INRA, UMR85 Physiologie de la Reproduction et des Comportements, Nouzilly 37380, France CNRS, UMR7247, Nouzilly 37380, France Université François Rabelais de Tours, Tours 37041, France IFCE, Nouzilly 37380, France.</Affiliation>
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<Initials>P</Initials>
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<Affiliation>INRA, UMR85 Physiologie de la Reproduction et des Comportements, Nouzilly 37380, France CNRS, UMR7247, Nouzilly 37380, France Université François Rabelais de Tours, Tours 37041, France IFCE, Nouzilly 37380, France.</Affiliation>
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<LastName>Locatelli</LastName>
<ForeName>Yann</ForeName>
<Initials>Y</Initials>
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<Affiliation>INRA, UMR85 Physiologie de la Reproduction et des Comportements, Nouzilly 37380, France MNHN, Laboratoire de la Réserve de la Haute Touche, Obterre 36290, France CNRS, UMR7247, Nouzilly 37380, France Université François Rabelais de Tours, Tours 37041, France IFCE, Nouzilly 37380, France.</Affiliation>
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<PublicationType UI="D016428">Journal Article</PublicationType>
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<Month>02</Month>
<Day>07</Day>
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<Country>England</Country>
<MedlineTA>Mol Hum Reprod</MedlineTA>
<NlmUniqueID>9513710</NlmUniqueID>
<ISSNLinking>1360-9947</ISSNLinking>
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<CitationSubset>IM</CitationSubset>
<KeywordList Owner="NOTNLM">
<Keyword MajorTopicYN="N">Foxo3a</Keyword>
<Keyword MajorTopicYN="N">anti-Mullerian hormone</Keyword>
<Keyword MajorTopicYN="N">c-Jun-N-terminal kinase</Keyword>
<Keyword MajorTopicYN="N">fertility preservation</Keyword>
<Keyword MajorTopicYN="N">in vitro ovarian tissue culture</Keyword>
<Keyword MajorTopicYN="N">primordial follicle activation</Keyword>
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<Year>2015</Year>
<Month>09</Month>
<Day>01</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted">
<Year>2016</Year>
<Month>01</Month>
<Day>20</Day>
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<Year>2016</Year>
<Month>2</Month>
<Day>25</Day>
<Hour>6</Hour>
<Minute>0</Minute>
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<Year>2016</Year>
<Month>2</Month>
<Day>26</Day>
<Hour>6</Hour>
<Minute>0</Minute>
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<Year>2016</Year>
<Month>2</Month>
<Day>26</Day>
<Hour>6</Hour>
<Minute>0</Minute>
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<PublicationStatus>ppublish</PublicationStatus>
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<ArticleId IdType="pubmed">26908644</ArticleId>
<ArticleId IdType="pii">gaw012</ArticleId>
<ArticleId IdType="doi">10.1093/molehr/gaw012</ArticleId>
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