Measuring Cell Death by Propidium Iodide Uptake and Flow Cytometry.
Identifieur interne : 001B54 ( PubMed/Curation ); précédent : 001B53; suivant : 001B55Measuring Cell Death by Propidium Iodide Uptake and Flow Cytometry.
Auteurs : Lisa C. Crowley [Australie] ; Adrian P. Scott [Australie] ; Brooke J. Marfell [Australie] ; Jeanne A. Boughaba [France] ; Grace Chojnowski [Australie] ; Nigel J. Waterhouse [Australie]Source :
- Cold Spring Harbor protocols [ 1559-6095 ] ; 2016.
Descripteurs français
- KwdFr :
- MESH :
- métabolisme : Colorants fluorescents, Intercalants, Propidium.
- Animaux, Coloration et marquage, Cytométrie en flux, Humains, Mort cellulaire.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Fluorescent Dyes, Intercalating Agents, Propidium.
- methods : Flow Cytometry, Staining and Labeling.
- Animals, Cell Death, Humans.
Abstract
Propidium iodide (PI) is a small fluorescent molecule that binds to DNA but cannot passively traverse into cells that possess an intact plasma membrane. PI uptake versus exclusion can be used to discriminate dead cells, in which plasma membranes become permeable regardless of the mechanism of death, from live cells with intact membranes. PI is excited by wavelengths between 400 and 600 nm and emits light between 600 and 700 nm, and is therefore compatible with lasers and photodetectors commonly available in flow cytometers. This protocol for PI staining can be used to quantitate cell death in most modern research facilities and universities.
DOI: 10.1101/pdb.prot087163
PubMed: 27371595
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Crowley</name><affiliation wicri:level="1"><nlm:affiliation>Apoptosis and Cytotoxicity Laboratory, Mater Research, Translational Research Institute, Woolloongabba, Brisbane, Queensland 4102, Australia;</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Apoptosis and Cytotoxicity Laboratory, Mater Research, Translational Research Institute, Woolloongabba, Brisbane, Queensland 4102</wicri:regionArea></affiliation></author><author><name sortKey="Scott, Adrian P" sort="Scott, Adrian P" uniqKey="Scott A" first="Adrian P" last="Scott">Adrian P. Scott</name><affiliation wicri:level="1"><nlm:affiliation>Apoptosis and Cytotoxicity Laboratory, Mater Research, Translational Research Institute, Woolloongabba, Brisbane, Queensland 4102, Australia;</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Apoptosis and Cytotoxicity Laboratory, Mater Research, Translational Research Institute, Woolloongabba, Brisbane, Queensland 4102</wicri:regionArea></affiliation></author><author><name sortKey="Marfell, Brooke J" sort="Marfell, Brooke J" uniqKey="Marfell B" first="Brooke J" last="Marfell">Brooke J. Marfell</name><affiliation wicri:level="1"><nlm:affiliation>Apoptosis and Cytotoxicity Laboratory, Mater Research, Translational Research Institute, Woolloongabba, Brisbane, Queensland 4102, Australia;</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Apoptosis and Cytotoxicity Laboratory, Mater Research, Translational Research Institute, Woolloongabba, Brisbane, Queensland 4102</wicri:regionArea></affiliation></author><author><name sortKey="Boughaba, Jeanne A" sort="Boughaba, Jeanne A" uniqKey="Boughaba J" first="Jeanne A" last="Boughaba">Jeanne A. 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Waterhouse</name><affiliation wicri:level="1"><nlm:affiliation>Apoptosis and Cytotoxicity Laboratory, Mater Research, Translational Research Institute, Woolloongabba, Brisbane, Queensland 4102, Australia; Flow Cytometry and Imaging, QIMR Berghofer Medical Research Institute, Herston, Brisbane, Queensland 4006, Australia; School of Medicine, University of Queensland, St. Lucia, Brisbane, Queensland 4072, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Apoptosis and Cytotoxicity Laboratory, Mater Research, Translational Research Institute, Woolloongabba, Brisbane, Queensland 4102, Australia; Flow Cytometry and Imaging, QIMR Berghofer Medical Research Institute, Herston, Brisbane, Queensland 4006, Australia; School of Medicine, University of Queensland, St. Lucia, Brisbane, Queensland 4072</wicri:regionArea></affiliation></author></analytic><series><title level="j">Cold Spring Harbor protocols</title><idno type="eISSN">1559-6095</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Death</term><term>Flow Cytometry (methods)</term><term>Fluorescent Dyes (metabolism)</term><term>Humans</term><term>Intercalating Agents (metabolism)</term><term>Propidium (metabolism)</term><term>Staining and Labeling (methods)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Colorants fluorescents (métabolisme)</term><term>Coloration et marquage ()</term><term>Cytométrie en flux ()</term><term>Humains</term><term>Intercalants (métabolisme)</term><term>Mort cellulaire</term><term>Propidium (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Fluorescent Dyes</term><term>Intercalating Agents</term><term>Propidium</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Flow Cytometry</term><term>Staining and Labeling</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Colorants fluorescents</term><term>Intercalants</term><term>Propidium</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Death</term><term>Humans</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Coloration et marquage</term><term>Cytométrie en flux</term><term>Humains</term><term>Mort cellulaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Propidium iodide (PI) is a small fluorescent molecule that binds to DNA but cannot passively traverse into cells that possess an intact plasma membrane. PI uptake versus exclusion can be used to discriminate dead cells, in which plasma membranes become permeable regardless of the mechanism of death, from live cells with intact membranes. PI is excited by wavelengths between 400 and 600 nm and emits light between 600 and 700 nm, and is therefore compatible with lasers and photodetectors commonly available in flow cytometers. This protocol for PI staining can be used to quantitate cell death in most modern research facilities and universities.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27371595</PMID><DateCreated><Year>2016</Year><Month>07</Month><Day>02</Day></DateCreated><DateCompleted><Year>2017</Year><Month>10</Month><Day>09</Day></DateCompleted><DateRevised><Year>2017</Year><Month>10</Month><Day>09</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1559-6095</ISSN><JournalIssue CitedMedium="Internet"><Volume>2016</Volume><Issue>7</Issue><PubDate><Year>2016</Year><Month>Jul</Month><Day>01</Day></PubDate></JournalIssue><Title>Cold Spring Harbor protocols</Title><ISOAbbreviation>Cold Spring Harb Protoc</ISOAbbreviation></Journal><ArticleTitle>Measuring Cell Death by Propidium Iodide Uptake and Flow Cytometry.</ArticleTitle><Pagination><MedlinePgn>pdb.prot087163</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1101/pdb.prot087163</ELocationID><Abstract><AbstractText>Propidium iodide (PI) is a small fluorescent molecule that binds to DNA but cannot passively traverse into cells that possess an intact plasma membrane. PI uptake versus exclusion can be used to discriminate dead cells, in which plasma membranes become permeable regardless of the mechanism of death, from live cells with intact membranes. PI is excited by wavelengths between 400 and 600 nm and emits light between 600 and 700 nm, and is therefore compatible with lasers and photodetectors commonly available in flow cytometers. This protocol for PI staining can be used to quantitate cell death in most modern research facilities and universities.</AbstractText><CopyrightInformation>© 2016 Cold Spring Harbor Laboratory Press.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Crowley</LastName><ForeName>Lisa C</ForeName><Initials>LC</Initials><AffiliationInfo><Affiliation>Apoptosis and Cytotoxicity Laboratory, Mater Research, Translational Research Institute, Woolloongabba, Brisbane, Queensland 4102, Australia;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Scott</LastName><ForeName>Adrian P</ForeName><Initials>AP</Initials><AffiliationInfo><Affiliation>Apoptosis and Cytotoxicity Laboratory, Mater Research, Translational Research Institute, Woolloongabba, Brisbane, Queensland 4102, Australia;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Marfell</LastName><ForeName>Brooke J</ForeName><Initials>BJ</Initials><AffiliationInfo><Affiliation>Apoptosis and Cytotoxicity Laboratory, Mater Research, Translational Research Institute, Woolloongabba, Brisbane, Queensland 4102, Australia;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Boughaba</LastName><ForeName>Jeanne A</ForeName><Initials>JA</Initials><AffiliationInfo><Affiliation>Apoptosis and Cytotoxicity Laboratory, Mater Research, Translational Research Institute, Woolloongabba, Brisbane, Queensland 4102, Australia; Agroparistech, Paris Cedex 05, France;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chojnowski</LastName><ForeName>Grace</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Flow Cytometry and Imaging, QIMR Berghofer Medical Research Institute, Herston, Brisbane, Queensland 4006, Australia;</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Waterhouse</LastName><ForeName>Nigel J</ForeName><Initials>NJ</Initials><AffiliationInfo><Affiliation>Apoptosis and Cytotoxicity Laboratory, Mater Research, Translational Research Institute, Woolloongabba, Brisbane, Queensland 4102, Australia; Flow Cytometry and Imaging, QIMR Berghofer Medical Research Institute, Herston, Brisbane, Queensland 4006, Australia; School of Medicine, University of Queensland, St. Lucia, Brisbane, Queensland 4072, Australia.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>07</Month><Day>01</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Cold Spring Harb Protoc</MedlineTA><NlmUniqueID>101524530</NlmUniqueID><ISSNLinking>1559-6095</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005456">Fluorescent Dyes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007364">Intercalating Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>36015-30-2</RegistryNumber><NameOfSubstance UI="D011419">Propidium</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016923" MajorTopicYN="Y">Cell Death</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005434" MajorTopicYN="N">Flow Cytometry</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005456" MajorTopicYN="N">Fluorescent Dyes</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007364" MajorTopicYN="N">Intercalating Agents</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011419" MajorTopicYN="N">Propidium</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013194" MajorTopicYN="N">Staining and Labeling</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>7</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>7</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>10</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27371595</ArticleId><ArticleId IdType="pii">2016/7/pdb.prot087163</ArticleId><ArticleId IdType="doi">10.1101/pdb.prot087163</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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