Influenza NA and PB1 Gene Segments Interact during the Formation of Viral Progeny: Localization of the Binding Region within the PB1 Gene.
Identifieur interne : 001A64 ( PubMed/Curation ); précédent : 001A63; suivant : 001A65Influenza NA and PB1 Gene Segments Interact during the Formation of Viral Progeny: Localization of the Binding Region within the PB1 Gene.
Auteurs : Brad Gilbertson [Australie] ; Tian Zheng [Australie] ; Marie Gerber [France] ; Anne Printz-Schweigert [France] ; Chi Ong [Australie] ; Roland Marquet [France] ; Catherine Isel [France] ; Steven Rockman [Australie] ; Lorena Brown [Australie]Source :
- Viruses [ 1999-4915 ] ; 2016.
Descripteurs français
- KwdFr :
- MESH :
- génétique : ARN viral, Protéines virales, Sialidase.
- métabolisme : ARN viral.
- physiologie : Virus de la grippe A.
- Analyse de mutations d'ADN, Animaux, Assemblage viral, Génétique inverse, Humains, Lignée cellulaire, Plasmides.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Neuraminidase, RNA, Viral, Viral Proteins.
- chemical , metabolism : RNA, Viral.
- physiology : Influenza A virus.
- Animals, Cell Line, DNA Mutational Analysis, Humans, Plasmids, Reverse Genetics, Virus Assembly.
Abstract
The influenza A virus genome comprises eight negative-sense viral RNAs (vRNAs) that form individual ribonucleoprotein (RNP) complexes. In order to incorporate a complete set of each of these vRNAs, the virus uses a selective packaging mechanism that facilitates co-packaging of specific gene segments but whose molecular basis is still not fully understood. Recently, we used a competitive transfection model where plasmids encoding the A/Puerto Rico/8/34 (PR8) and A/Udorn/307/72 (Udorn) PB1 gene segments were competed to show that the Udorn PB1 gene segment is preferentially co-packaged into progeny virions with the Udorn NA gene segment. Here we created chimeric PB1 genes combining both Udorn and PR8 PB1 sequences to further define the location within the Udorn PB1 gene that drives co-segregation of these genes and show that nucleotides 1776-2070 of the PB1 gene are crucial for preferential selection. In vitro assays examining specific interactions between Udorn NA vRNA and purified vRNAs transcribed from chimeric PB1 genes also supported the importance of this region in the PB1-NA interaction. Hence, this work identifies an association between viral genes that are co-selected during packaging. It also reveals a region potentially important in the RNP-RNP interactions within the supramolecular complex that is predicted to form prior to budding to allow one of each segment to be packaged in the viral progeny. Our study lays the foundation to understand the co-selection of specific genes, which may be critical to the emergence of new viruses with pandemic potential.
DOI: 10.3390/v8080238
PubMed: 27556479
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :001A88
Links to Exploration step
pubmed:27556479Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Influenza NA and PB1 Gene Segments Interact during the Formation of Viral Progeny: Localization of the Binding Region within the PB1 Gene.</title><author><name sortKey="Gilbertson, Brad" sort="Gilbertson, Brad" uniqKey="Gilbertson B" first="Brad" last="Gilbertson">Brad Gilbertson</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Parkville 3010, Victoria, Australia. bg@unimelb.edu.au.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Parkville 3010, Victoria</wicri:regionArea></affiliation></author><author><name sortKey="Zheng, Tian" sort="Zheng, Tian" uniqKey="Zheng T" first="Tian" last="Zheng">Tian Zheng</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Parkville 3010, Victoria, Australia. tianchi.zheng@gmail.com.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Parkville 3010, Victoria</wicri:regionArea></affiliation></author><author><name sortKey="Gerber, Marie" sort="Gerber, Marie" uniqKey="Gerber M" first="Marie" last="Gerber">Marie Gerber</name><affiliation wicri:level="1"><nlm:affiliation>Architecture et Réactivité de l'ARN, Université de Strasbourg, CNRS, IBMC, 15 rue René Descartes, Strasbourg 67084, France. m.gerber@ibmc-cnrs.unistra.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Architecture et Réactivité de l'ARN, Université de Strasbourg, CNRS, IBMC, 15 rue René Descartes, Strasbourg 67084</wicri:regionArea></affiliation></author><author><name sortKey="Printz Schweigert, Anne" sort="Printz Schweigert, Anne" uniqKey="Printz Schweigert A" first="Anne" last="Printz-Schweigert">Anne Printz-Schweigert</name><affiliation wicri:level="1"><nlm:affiliation>Architecture et Réactivité de l'ARN, Université de Strasbourg, CNRS, IBMC, 15 rue René Descartes, Strasbourg 67084, France. A.schweigert@ibmc-cnrs.unistra.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Architecture et Réactivité de l'ARN, Université de Strasbourg, CNRS, IBMC, 15 rue René Descartes, Strasbourg 67084</wicri:regionArea></affiliation></author><author><name sortKey="Ong, Chi" sort="Ong, Chi" uniqKey="Ong C" first="Chi" last="Ong">Chi Ong</name><affiliation wicri:level="1"><nlm:affiliation>Seqirus, 63 Poplar Rd, Parkville 3052, Victoria, Australia. Chi.Ong@seqirus.com.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Seqirus, 63 Poplar Rd, Parkville 3052, Victoria</wicri:regionArea></affiliation></author><author><name sortKey="Marquet, Roland" sort="Marquet, Roland" uniqKey="Marquet R" first="Roland" last="Marquet">Roland Marquet</name><affiliation wicri:level="1"><nlm:affiliation>Architecture et Réactivité de l'ARN, Université de Strasbourg, CNRS, IBMC, 15 rue René Descartes, Strasbourg 67084, France. r.marquet@ibmc-cnrs.unistra.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Architecture et Réactivité de l'ARN, Université de Strasbourg, CNRS, IBMC, 15 rue René Descartes, Strasbourg 67084</wicri:regionArea></affiliation></author><author><name sortKey="Isel, Catherine" sort="Isel, Catherine" uniqKey="Isel C" first="Catherine" last="Isel">Catherine Isel</name><affiliation wicri:level="1"><nlm:affiliation>Architecture et Réactivité de l'ARN, Université de Strasbourg, CNRS, IBMC, 15 rue René Descartes, Strasbourg 67084, France. c.isel@ibmc-cnrs.unistra.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Architecture et Réactivité de l'ARN, Université de Strasbourg, CNRS, IBMC, 15 rue René Descartes, Strasbourg 67084</wicri:regionArea></affiliation></author><author><name sortKey="Rockman, Steven" sort="Rockman, Steven" uniqKey="Rockman S" first="Steven" last="Rockman">Steven Rockman</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Parkville 3010, Victoria, Australia. Steve.Rockman@seqirus.com.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Parkville 3010, Victoria</wicri:regionArea></affiliation></author><author><name sortKey="Brown, Lorena" sort="Brown, Lorena" uniqKey="Brown L" first="Lorena" last="Brown">Lorena Brown</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Parkville 3010, Victoria, Australia. lorena@unimelb.edu.au.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Parkville 3010, Victoria</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:27556479</idno><idno type="pmid">27556479</idno><idno type="doi">10.3390/v8080238</idno><idno type="wicri:Area/PubMed/Corpus">001A88</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001A88</idno><idno type="wicri:Area/PubMed/Curation">001A64</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001A64</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Influenza NA and PB1 Gene Segments Interact during the Formation of Viral Progeny: Localization of the Binding Region within the PB1 Gene.</title><author><name sortKey="Gilbertson, Brad" sort="Gilbertson, Brad" uniqKey="Gilbertson B" first="Brad" last="Gilbertson">Brad Gilbertson</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Parkville 3010, Victoria, Australia. bg@unimelb.edu.au.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Parkville 3010, Victoria</wicri:regionArea></affiliation></author><author><name sortKey="Zheng, Tian" sort="Zheng, Tian" uniqKey="Zheng T" first="Tian" last="Zheng">Tian Zheng</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Parkville 3010, Victoria, Australia. tianchi.zheng@gmail.com.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Parkville 3010, Victoria</wicri:regionArea></affiliation></author><author><name sortKey="Gerber, Marie" sort="Gerber, Marie" uniqKey="Gerber M" first="Marie" last="Gerber">Marie Gerber</name><affiliation wicri:level="1"><nlm:affiliation>Architecture et Réactivité de l'ARN, Université de Strasbourg, CNRS, IBMC, 15 rue René Descartes, Strasbourg 67084, France. m.gerber@ibmc-cnrs.unistra.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Architecture et Réactivité de l'ARN, Université de Strasbourg, CNRS, IBMC, 15 rue René Descartes, Strasbourg 67084</wicri:regionArea></affiliation></author><author><name sortKey="Printz Schweigert, Anne" sort="Printz Schweigert, Anne" uniqKey="Printz Schweigert A" first="Anne" last="Printz-Schweigert">Anne Printz-Schweigert</name><affiliation wicri:level="1"><nlm:affiliation>Architecture et Réactivité de l'ARN, Université de Strasbourg, CNRS, IBMC, 15 rue René Descartes, Strasbourg 67084, France. A.schweigert@ibmc-cnrs.unistra.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Architecture et Réactivité de l'ARN, Université de Strasbourg, CNRS, IBMC, 15 rue René Descartes, Strasbourg 67084</wicri:regionArea></affiliation></author><author><name sortKey="Ong, Chi" sort="Ong, Chi" uniqKey="Ong C" first="Chi" last="Ong">Chi Ong</name><affiliation wicri:level="1"><nlm:affiliation>Seqirus, 63 Poplar Rd, Parkville 3052, Victoria, Australia. Chi.Ong@seqirus.com.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Seqirus, 63 Poplar Rd, Parkville 3052, Victoria</wicri:regionArea></affiliation></author><author><name sortKey="Marquet, Roland" sort="Marquet, Roland" uniqKey="Marquet R" first="Roland" last="Marquet">Roland Marquet</name><affiliation wicri:level="1"><nlm:affiliation>Architecture et Réactivité de l'ARN, Université de Strasbourg, CNRS, IBMC, 15 rue René Descartes, Strasbourg 67084, France. r.marquet@ibmc-cnrs.unistra.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Architecture et Réactivité de l'ARN, Université de Strasbourg, CNRS, IBMC, 15 rue René Descartes, Strasbourg 67084</wicri:regionArea></affiliation></author><author><name sortKey="Isel, Catherine" sort="Isel, Catherine" uniqKey="Isel C" first="Catherine" last="Isel">Catherine Isel</name><affiliation wicri:level="1"><nlm:affiliation>Architecture et Réactivité de l'ARN, Université de Strasbourg, CNRS, IBMC, 15 rue René Descartes, Strasbourg 67084, France. c.isel@ibmc-cnrs.unistra.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Architecture et Réactivité de l'ARN, Université de Strasbourg, CNRS, IBMC, 15 rue René Descartes, Strasbourg 67084</wicri:regionArea></affiliation></author><author><name sortKey="Rockman, Steven" sort="Rockman, Steven" uniqKey="Rockman S" first="Steven" last="Rockman">Steven Rockman</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Parkville 3010, Victoria, Australia. Steve.Rockman@seqirus.com.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Parkville 3010, Victoria</wicri:regionArea></affiliation></author><author><name sortKey="Brown, Lorena" sort="Brown, Lorena" uniqKey="Brown L" first="Lorena" last="Brown">Lorena Brown</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Parkville 3010, Victoria, Australia. lorena@unimelb.edu.au.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Parkville 3010, Victoria</wicri:regionArea></affiliation></author></analytic><series><title level="j">Viruses</title><idno type="eISSN">1999-4915</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>DNA Mutational Analysis</term><term>Humans</term><term>Influenza A virus (physiology)</term><term>Neuraminidase (genetics)</term><term>Plasmids</term><term>RNA, Viral (genetics)</term><term>RNA, Viral (metabolism)</term><term>Reverse Genetics</term><term>Viral Proteins (genetics)</term><term>Virus Assembly</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (génétique)</term><term>ARN viral (métabolisme)</term><term>Analyse de mutations d'ADN</term><term>Animaux</term><term>Assemblage viral</term><term>Génétique inverse</term><term>Humains</term><term>Lignée cellulaire</term><term>Plasmides</term><term>Protéines virales (génétique)</term><term>Sialidase (génétique)</term><term>Virus de la grippe A (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Neuraminidase</term><term>RNA, Viral</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term><term>Protéines virales</term><term>Sialidase</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN viral</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>DNA Mutational Analysis</term><term>Humans</term><term>Plasmids</term><term>Reverse Genetics</term><term>Virus Assembly</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de mutations d'ADN</term><term>Animaux</term><term>Assemblage viral</term><term>Génétique inverse</term><term>Humains</term><term>Lignée cellulaire</term><term>Plasmides</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The influenza A virus genome comprises eight negative-sense viral RNAs (vRNAs) that form individual ribonucleoprotein (RNP) complexes. In order to incorporate a complete set of each of these vRNAs, the virus uses a selective packaging mechanism that facilitates co-packaging of specific gene segments but whose molecular basis is still not fully understood. Recently, we used a competitive transfection model where plasmids encoding the A/Puerto Rico/8/34 (PR8) and A/Udorn/307/72 (Udorn) PB1 gene segments were competed to show that the Udorn PB1 gene segment is preferentially co-packaged into progeny virions with the Udorn NA gene segment. Here we created chimeric PB1 genes combining both Udorn and PR8 PB1 sequences to further define the location within the Udorn PB1 gene that drives co-segregation of these genes and show that nucleotides 1776-2070 of the PB1 gene are crucial for preferential selection. In vitro assays examining specific interactions between Udorn NA vRNA and purified vRNAs transcribed from chimeric PB1 genes also supported the importance of this region in the PB1-NA interaction. Hence, this work identifies an association between viral genes that are co-selected during packaging. It also reveals a region potentially important in the RNP-RNP interactions within the supramolecular complex that is predicted to form prior to budding to allow one of each segment to be packaged in the viral progeny. Our study lays the foundation to understand the co-selection of specific genes, which may be critical to the emergence of new viruses with pandemic potential.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27556479</PMID><DateCreated><Year>2016</Year><Month>08</Month><Day>25</Day></DateCreated><DateCompleted><Year>2017</Year><Month>09</Month><Day>26</Day></DateCompleted><DateRevised><Year>2017</Year><Month>09</Month><Day>26</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1999-4915</ISSN><JournalIssue CitedMedium="Internet"><Volume>8</Volume><Issue>8</Issue><PubDate><Year>2016</Year><Month>Aug</Month><Day>20</Day></PubDate></JournalIssue><Title>Viruses</Title><ISOAbbreviation>Viruses</ISOAbbreviation></Journal><ArticleTitle>Influenza NA and PB1 Gene Segments Interact during the Formation of Viral Progeny: Localization of the Binding Region within the PB1 Gene.</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.3390/v8080238</ELocationID><ELocationID EIdType="pii" ValidYN="Y">E238</ELocationID><Abstract><AbstractText>The influenza A virus genome comprises eight negative-sense viral RNAs (vRNAs) that form individual ribonucleoprotein (RNP) complexes. In order to incorporate a complete set of each of these vRNAs, the virus uses a selective packaging mechanism that facilitates co-packaging of specific gene segments but whose molecular basis is still not fully understood. Recently, we used a competitive transfection model where plasmids encoding the A/Puerto Rico/8/34 (PR8) and A/Udorn/307/72 (Udorn) PB1 gene segments were competed to show that the Udorn PB1 gene segment is preferentially co-packaged into progeny virions with the Udorn NA gene segment. Here we created chimeric PB1 genes combining both Udorn and PR8 PB1 sequences to further define the location within the Udorn PB1 gene that drives co-segregation of these genes and show that nucleotides 1776-2070 of the PB1 gene are crucial for preferential selection. In vitro assays examining specific interactions between Udorn NA vRNA and purified vRNAs transcribed from chimeric PB1 genes also supported the importance of this region in the PB1-NA interaction. Hence, this work identifies an association between viral genes that are co-selected during packaging. It also reveals a region potentially important in the RNP-RNP interactions within the supramolecular complex that is predicted to form prior to budding to allow one of each segment to be packaged in the viral progeny. Our study lays the foundation to understand the co-selection of specific genes, which may be critical to the emergence of new viruses with pandemic potential.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Gilbertson</LastName><ForeName>Brad</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Parkville 3010, Victoria, Australia. bg@unimelb.edu.au.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zheng</LastName><ForeName>Tian</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Parkville 3010, Victoria, Australia. tianchi.zheng@gmail.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gerber</LastName><ForeName>Marie</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Architecture et Réactivité de l'ARN, Université de Strasbourg, CNRS, IBMC, 15 rue René Descartes, Strasbourg 67084, France. m.gerber@ibmc-cnrs.unistra.fr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Printz-Schweigert</LastName><ForeName>Anne</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Architecture et Réactivité de l'ARN, Université de Strasbourg, CNRS, IBMC, 15 rue René Descartes, Strasbourg 67084, France. A.schweigert@ibmc-cnrs.unistra.fr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ong</LastName><ForeName>Chi</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Seqirus, 63 Poplar Rd, Parkville 3052, Victoria, Australia. Chi.Ong@seqirus.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Marquet</LastName><ForeName>Roland</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Architecture et Réactivité de l'ARN, Université de Strasbourg, CNRS, IBMC, 15 rue René Descartes, Strasbourg 67084, France. r.marquet@ibmc-cnrs.unistra.fr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Isel</LastName><ForeName>Catherine</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Architecture et Réactivité de l'ARN, Université de Strasbourg, CNRS, IBMC, 15 rue René Descartes, Strasbourg 67084, France. c.isel@ibmc-cnrs.unistra.fr.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Unité de Génétique Moléculaire des Virus à ARN, Département de virologie, Institut Pasteur, Paris 75005, France. c.isel@ibmc-cnrs.unistra.fr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rockman</LastName><ForeName>Steven</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Parkville 3010, Victoria, Australia. Steve.Rockman@seqirus.com.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Seqirus, 63 Poplar Rd, Parkville 3052, Victoria, Australia. Steve.Rockman@seqirus.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Brown</LastName><ForeName>Lorena</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Parkville 3010, Victoria, Australia. lorena@unimelb.edu.au.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>08</Month><Day>20</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Viruses</MedlineTA><NlmUniqueID>101509722</NlmUniqueID><ISSNLinking>1999-4915</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014764">Viral Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C077346">influenza virus polymerase basic protein 1</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.18</RegistryNumber><NameOfSubstance UI="C487630">NA protein, influenza A virus</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.18</RegistryNumber><NameOfSubstance UI="D009439">Neuraminidase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="Cites"><RefSource>J Virol. 2008 Mar;82(5):2295-304</RefSource><PMID Version="1">18094182</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>PLoS One. 2009 Jul 28;4(7):e6402</RefSource><PMID Version="1">19636415</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nature. 2006 Jan 26;439(7075):490-2</RefSource><PMID Version="1">16437116</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nucleic Acids Res. 2012 Mar;40(5):2197-209</RefSource><PMID Version="1">22075989</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Proc Natl Acad Sci U S A. 1987 Nov;84(22):8140-4</RefSource><PMID Version="1">2446318</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>PLoS Pathog. 2013;9(5):e1003392</RefSource><PMID Version="1">23737748</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Proc Natl Acad Sci U S A. 2010 Mar 9;107(10):4687-92</RefSource><PMID Version="1">20176961</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Virology. 2002 Apr 10;295(2):250-5</RefSource><PMID Version="1">12033783</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nucleic Acids Res. 2013 Jan;41(2):1241-54</RefSource><PMID Version="1">23221636</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>EMBO J. 1999 Jul 1;18(13):3767-75</RefSource><PMID Version="1">10393191</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Infect Immun. 1984 Feb;43(2):457-62</RefSource><PMID Version="1">6693167</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Virol. 2009 Aug;83(16):8131-40</RefSource><PMID Version="1">19493997</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Virol. 2011 Aug;85(16):8449-52</RefSource><PMID Version="1">21680507</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Virol. 1989 Nov;63(11):4603-8</RefSource><PMID Version="1">2795713</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Virology. 1969 Oct;39(2):250-9</RefSource><PMID Version="1">4186524</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Virol. 2012 Sep;86(18):10200-2</RefSource><PMID Version="1">22740412</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Virol. 2013 May;87(10):5577-85</RefSource><PMID Version="1">23468502</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Virol. 2008 Dec;82(23):11869-79</RefSource><PMID Version="1">18815307</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Virology. 1978 Jun 1;87(1):13-20</RefSource><PMID Version="1">664248</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>PLoS Curr. 2010 Sep 05;2:RRN1165</RefSource><PMID Version="1">20802842</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nat Commun. 2012 Jan 24;3:639</RefSource><PMID Version="1">22273677</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Proc Natl Acad Sci U S A. 2013 Oct 1;110(40):E3840-8</RefSource><PMID Version="1">24043788</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>PLoS One. 2011;6(6):e20823</RefSource><PMID Version="1">21695145</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Virol. 2008 Dec;82(23):11880-8</RefSource><PMID Version="1">18815312</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>EMBO J. 1989 Dec 1;8(12):3867-74</RefSource><PMID Version="1">2555175</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Virol. 1994 Mar;68(3):1819-26</RefSource><PMID Version="1">8107244</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Gen Virol. 2010 Feb;91(Pt 2):313-28</RefSource><PMID Version="1">19955561</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Arch Virol. 2001 Dec;146(12):2275-89</RefSource><PMID Version="1">11811679</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Virol. 2010 Oct;84(20):10918-22</RefSource><PMID Version="1">20686037</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nat Biotechnol. 2002 Nov;20(11):1151-4</RefSource><PMID Version="1">12355096</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Proc Natl Acad Sci U S A. 2006 Dec 12;103(50):19123-7</RefSource><PMID Version="1">17146053</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>PLoS Pathog. 2013;9(6):e1003421</RefSource><PMID Version="1">23785286</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Trends Microbiol. 2014 Aug;22(8):446-55</RefSource><PMID Version="1">24798745</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Virol. 1972 Oct;10(4):795-800</RefSource><PMID Version="1">4117350</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Virol. 2005 Mar;79(6):3766-74</RefSource><PMID Version="1">15731270</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Proc Natl Acad Sci U S A. 2000 May 23;97(11):6108-13</RefSource><PMID Version="1">10801978</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>PLoS Pathog. 2008 May 23;4(5):e1000072</RefSource><PMID Version="1">18497857</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nat Rev Microbiol. 2015 Jan;13(1):28-41</RefSource><PMID Version="1">25417656</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Vaccine. 2012 Dec 7;30(51):7359-67</RefSource><PMID Version="1">23063835</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nature. 2014 Dec 18;516(7531):355-60</RefSource><PMID Version="1">25409142</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nat Med. 2001 Dec;7(12):1306-12</RefSource><PMID Version="1">11726970</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Annu Rev Microbiol. 2008;62:403-24</RefSource><PMID Version="1">18785841</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):9101-6</RefSource><PMID Version="1">22547828</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Virol. 2007 Sep;81(18):9727-36</RefSource><PMID Version="1">17634232</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Gen Virol. 2013 Nov;94(Pt 11):2406-16</RefSource><PMID Version="1">23939981</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Virol. 2014 Aug;88(16):8971-80</RefSource><PMID Version="1">24872588</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Mol Cell Biol. 1990 Aug;10(8):4139-45</RefSource><PMID Version="1">2196448</PMID></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004252" MajorTopicYN="N">DNA Mutational Analysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009980" MajorTopicYN="N">Influenza A virus</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009439" MajorTopicYN="N">Neuraminidase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010957" MajorTopicYN="N">Plasmids</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D059386" MajorTopicYN="N">Reverse Genetics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014764" MajorTopicYN="N">Viral Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019065" MajorTopicYN="Y">Virus Assembly</DescriptorName></MeshHeading></MeshHeadingList><OtherID Source="NLM">PMC4997600</OtherID><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">RNA-RNA interaction</Keyword><Keyword MajorTopicYN="N">competitive transfection</Keyword><Keyword MajorTopicYN="N">gene segments</Keyword><Keyword MajorTopicYN="N">influenza virus</Keyword><Keyword MajorTopicYN="N">packaging</Keyword><Keyword MajorTopicYN="N">reassortment</Keyword><Keyword MajorTopicYN="N">viral polymerase</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>06</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2016</Year><Month>08</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>08</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>8</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>8</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>9</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27556479</ArticleId><ArticleId IdType="pii">v8080238</ArticleId><ArticleId IdType="doi">10.3390/v8080238</ArticleId><ArticleId IdType="pmc">PMC4997600</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/PubMed/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001A64 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd -nk 001A64 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Asie
|area= AustralieFrV1
|flux= PubMed
|étape= Curation
|type= RBID
|clé= pubmed:27556479
|texte= Influenza NA and PB1 Gene Segments Interact during the Formation of Viral Progeny: Localization of the Binding Region within the PB1 Gene.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Curation/RBID.i -Sk "pubmed:27556479" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd \
| NlmPubMed2Wicri -a AustralieFrV1
| This area was generated with Dilib version V0.6.33. |  |