The aliphatic amidase AmiE is involved in regulation of Pseudomonas aeruginosa virulence.
Identifieur interne : 001206 ( PubMed/Curation ); précédent : 001205; suivant : 001207The aliphatic amidase AmiE is involved in regulation of Pseudomonas aeruginosa virulence.
Auteurs : Thomas Clamens [France] ; Thibaut Rosay [France] ; Alexandre Crépin [France] ; Teddy Grandjean [France] ; Takfarinas Kentache [France] ; Julie Hardouin [France] ; Perrine Bortolotti [France] ; Anke Neidig [Allemagne] ; Marlies Mooij [Irlande (pays)] ; Mélanie Hillion [France] ; Julien Vieillard [France] ; Pascal Cosette [France] ; Joerg Overhage [Allemagne] ; Fergal O'Gara [Irlande (pays)] ; Emeline Bouffartigues [France] ; Alain Dufour [France] ; Sylvie Chevalier [France] ; Benoit Guery [France] ; Pierre Cornelis [France] ; Marc G J. Feuilloley [France] ; Olivier Lesouhaitier [France]Source :
- Scientific reports [ 2045-2322 ] ; 2017.
Abstract
We have previously shown that the eukaryotic C-type natriuretic peptide hormone (CNP) regulates Pseudomonas aeruginosa virulence and biofilm formation after binding on the AmiC sensor, triggering the amiE transcription. Herein, the involvement of the aliphatic amidase AmiE in P. aeruginosa virulence regulation has been investigated. The proteome analysis of an AmiE over-producing strain (AmiE(+)) revealed an expression change for 138 proteins, including some that are involved in motility, synthesis of quorum sensing compounds and virulence regulation. We observed that the AmiE(+) strain produced less biofilm compared to the wild type, and over-produced rhamnolipids. In the same line, AmiE is involved in P. aeruginosa motilities (swarming and twitching) and production of the quorum sensing molecules N-acyl homoserine lactones and Pseudomonas Quinolone Signal (PQS). We observed that AmiE overproduction reduced levels of HCN and pyocyanin causing a decreased virulence in different hosts (i.e. Dictyostelium discoideum and Caenorhabditis elegans). This phenotype was further confirmed in a mouse model of acute lung infection, in which AmiE overproduction resulted in an almost fully virulence decrease. Taken together, our data suggest that, in addition to its role in bacterial secondary metabolism, AmiE is involved in P. aeruginosa virulence regulation by modulating pilus synthesis and cell-to-cell communication.
DOI: 10.1038/srep41178
PubMed: 28117457
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Bretagne-Sud, EA 3884, LBCM, IUEM, Lorient, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Univ. Bretagne-Sud, EA 3884, LBCM, IUEM, Lorient</wicri:regionArea></affiliation></author><author><name sortKey="Chevalier, Sylvie" sort="Chevalier, Sylvie" uniqKey="Chevalier S" first="Sylvie" last="Chevalier">Sylvie Chevalier</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN, Evreux, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN, Evreux</wicri:regionArea></affiliation></author><author><name sortKey="Guery, Benoit" sort="Guery, Benoit" uniqKey="Guery B" first="Benoit" last="Guery">Benoit Guery</name><affiliation wicri:level="1"><nlm:affiliation>Univ. Lille, CHU Lille, EA 7366 - Recherche Translationnelle: relations hôte pathogènes, Lille, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Univ. Lille, CHU Lille, EA 7366 - Recherche Translationnelle: relations hôte pathogènes, Lille</wicri:regionArea></affiliation></author><author><name sortKey="Cornelis, Pierre" sort="Cornelis, Pierre" uniqKey="Cornelis P" first="Pierre" last="Cornelis">Pierre Cornelis</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN, Evreux, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN, Evreux</wicri:regionArea></affiliation></author><author><name sortKey="Feuilloley, Marc G J" sort="Feuilloley, Marc G J" uniqKey="Feuilloley M" first="Marc G J" last="Feuilloley">Marc G J. Feuilloley</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN, Evreux, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN, Evreux</wicri:regionArea></affiliation></author><author><name sortKey="Lesouhaitier, Olivier" sort="Lesouhaitier, Olivier" uniqKey="Lesouhaitier O" first="Olivier" last="Lesouhaitier">Olivier Lesouhaitier</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN, Evreux, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN, Evreux</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:28117457</idno><idno type="pmid">28117457</idno><idno type="doi">10.1038/srep41178</idno><idno type="wicri:Area/PubMed/Corpus">001217</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001217</idno><idno type="wicri:Area/PubMed/Curation">001206</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001206</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The aliphatic amidase AmiE is involved in regulation of Pseudomonas aeruginosa virulence.</title><author><name sortKey="Clamens, Thomas" sort="Clamens, Thomas" uniqKey="Clamens T" first="Thomas" last="Clamens">Thomas Clamens</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN, Evreux, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN, Evreux</wicri:regionArea></affiliation></author><author><name sortKey="Rosay, Thibaut" sort="Rosay, Thibaut" uniqKey="Rosay T" first="Thibaut" last="Rosay">Thibaut Rosay</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN, Evreux, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN, Evreux</wicri:regionArea></affiliation></author><author><name sortKey="Crepin, Alexandre" sort="Crepin, Alexandre" uniqKey="Crepin A" first="Alexandre" last="Crépin">Alexandre Crépin</name><affiliation wicri:level="1"><nlm:affiliation>Univ. Bretagne-Sud, EA 3884, LBCM, IUEM, Lorient, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Univ. Bretagne-Sud, EA 3884, LBCM, IUEM, Lorient</wicri:regionArea></affiliation></author><author><name sortKey="Grandjean, Teddy" sort="Grandjean, Teddy" uniqKey="Grandjean T" first="Teddy" last="Grandjean">Teddy Grandjean</name><affiliation wicri:level="1"><nlm:affiliation>Univ. Lille, CHU Lille, EA 7366 - Recherche Translationnelle: relations hôte pathogènes, Lille, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Univ. Lille, CHU Lille, EA 7366 - Recherche Translationnelle: relations hôte pathogènes, Lille</wicri:regionArea></affiliation></author><author><name sortKey="Kentache, Takfarinas" sort="Kentache, Takfarinas" uniqKey="Kentache T" first="Takfarinas" last="Kentache">Takfarinas Kentache</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory « Polymères, Biopolymères, Surfaces » (UMR 6270 CNRS), Proteomic Platform PISSARO, Normandie Univ, UNIROUEN, Mont-Saint-Aignan, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratory « Polymères, Biopolymères, Surfaces » (UMR 6270 CNRS), Proteomic Platform PISSARO, Normandie Univ, UNIROUEN, Mont-Saint-Aignan</wicri:regionArea></affiliation></author><author><name sortKey="Hardouin, Julie" sort="Hardouin, Julie" uniqKey="Hardouin J" first="Julie" last="Hardouin">Julie Hardouin</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory « Polymères, Biopolymères, Surfaces » (UMR 6270 CNRS), Proteomic Platform PISSARO, Normandie Univ, UNIROUEN, Mont-Saint-Aignan, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratory « Polymères, Biopolymères, Surfaces » (UMR 6270 CNRS), Proteomic Platform PISSARO, Normandie Univ, UNIROUEN, Mont-Saint-Aignan</wicri:regionArea></affiliation></author><author><name sortKey="Bortolotti, Perrine" sort="Bortolotti, Perrine" uniqKey="Bortolotti P" first="Perrine" last="Bortolotti">Perrine Bortolotti</name><affiliation wicri:level="1"><nlm:affiliation>Univ. Lille, CHU Lille, EA 7366 - Recherche Translationnelle: relations hôte pathogènes, Lille, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Univ. Lille, CHU Lille, EA 7366 - Recherche Translationnelle: relations hôte pathogènes, Lille</wicri:regionArea></affiliation></author><author><name sortKey="Neidig, Anke" sort="Neidig, Anke" uniqKey="Neidig A" first="Anke" last="Neidig">Anke Neidig</name><affiliation wicri:level="1"><nlm:affiliation>Karlsruhe Institute of Technology (KIT), Institute of Functional Interfaces, PO Box 3640, Karlsruhe, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Karlsruhe Institute of Technology (KIT), Institute of Functional Interfaces, PO Box 3640, Karlsruhe</wicri:regionArea></affiliation></author><author><name sortKey="Mooij, Marlies" sort="Mooij, Marlies" uniqKey="Mooij M" first="Marlies" last="Mooij">Marlies Mooij</name><affiliation wicri:level="1"><nlm:affiliation>BIOMERIT Research Centre, University College Cork, Cork, Ireland.</nlm:affiliation><country xml:lang="fr">Irlande (pays)</country><wicri:regionArea>BIOMERIT Research Centre, University College Cork, Cork</wicri:regionArea></affiliation></author><author><name sortKey="Hillion, Melanie" sort="Hillion, Melanie" uniqKey="Hillion M" first="Mélanie" last="Hillion">Mélanie Hillion</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN, Evreux, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN, Evreux</wicri:regionArea></affiliation></author><author><name sortKey="Vieillard, Julien" sort="Vieillard, Julien" uniqKey="Vieillard J" first="Julien" last="Vieillard">Julien Vieillard</name><affiliation wicri:level="1"><nlm:affiliation>Normandie Univ, UNIROUEN, INSA Rouen, CNRS, COBRA (UMR 6014), Evreux, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Normandie Univ, UNIROUEN, INSA Rouen, CNRS, COBRA (UMR 6014), Evreux</wicri:regionArea></affiliation></author><author><name sortKey="Cosette, Pascal" sort="Cosette, Pascal" uniqKey="Cosette P" first="Pascal" last="Cosette">Pascal Cosette</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory « Polymères, Biopolymères, Surfaces » (UMR 6270 CNRS), Proteomic Platform PISSARO, Normandie Univ, UNIROUEN, Mont-Saint-Aignan, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratory « Polymères, Biopolymères, Surfaces » (UMR 6270 CNRS), Proteomic Platform PISSARO, Normandie Univ, UNIROUEN, Mont-Saint-Aignan</wicri:regionArea></affiliation></author><author><name sortKey="Overhage, Joerg" sort="Overhage, Joerg" uniqKey="Overhage J" first="Joerg" last="Overhage">Joerg Overhage</name><affiliation wicri:level="1"><nlm:affiliation>Karlsruhe Institute of Technology (KIT), Institute of Functional Interfaces, PO Box 3640, Karlsruhe, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Karlsruhe Institute of Technology (KIT), Institute of Functional Interfaces, PO Box 3640, Karlsruhe</wicri:regionArea></affiliation></author><author><name sortKey="O Gara, Fergal" sort="O Gara, Fergal" uniqKey="O Gara F" first="Fergal" last="O'Gara">Fergal O'Gara</name><affiliation wicri:level="1"><nlm:affiliation>BIOMERIT Research Centre, University College Cork, Cork, Ireland.</nlm:affiliation><country xml:lang="fr">Irlande (pays)</country><wicri:regionArea>BIOMERIT Research Centre, University College Cork, Cork</wicri:regionArea></affiliation></author><author><name sortKey="Bouffartigues, Emeline" sort="Bouffartigues, Emeline" uniqKey="Bouffartigues E" first="Emeline" last="Bouffartigues">Emeline Bouffartigues</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN, Evreux, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN, Evreux</wicri:regionArea></affiliation></author><author><name sortKey="Dufour, Alain" sort="Dufour, Alain" uniqKey="Dufour A" first="Alain" last="Dufour">Alain Dufour</name><affiliation wicri:level="1"><nlm:affiliation>Univ. Bretagne-Sud, EA 3884, LBCM, IUEM, Lorient, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Univ. Bretagne-Sud, EA 3884, LBCM, IUEM, Lorient</wicri:regionArea></affiliation></author><author><name sortKey="Chevalier, Sylvie" sort="Chevalier, Sylvie" uniqKey="Chevalier S" first="Sylvie" last="Chevalier">Sylvie Chevalier</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN, Evreux, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN, Evreux</wicri:regionArea></affiliation></author><author><name sortKey="Guery, Benoit" sort="Guery, Benoit" uniqKey="Guery B" first="Benoit" last="Guery">Benoit Guery</name><affiliation wicri:level="1"><nlm:affiliation>Univ. Lille, CHU Lille, EA 7366 - Recherche Translationnelle: relations hôte pathogènes, Lille, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Univ. Lille, CHU Lille, EA 7366 - Recherche Translationnelle: relations hôte pathogènes, Lille</wicri:regionArea></affiliation></author><author><name sortKey="Cornelis, Pierre" sort="Cornelis, Pierre" uniqKey="Cornelis P" first="Pierre" last="Cornelis">Pierre Cornelis</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN, Evreux, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN, Evreux</wicri:regionArea></affiliation></author><author><name sortKey="Feuilloley, Marc G J" sort="Feuilloley, Marc G J" uniqKey="Feuilloley M" first="Marc G J" last="Feuilloley">Marc G J. Feuilloley</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN, Evreux, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN, Evreux</wicri:regionArea></affiliation></author><author><name sortKey="Lesouhaitier, Olivier" sort="Lesouhaitier, Olivier" uniqKey="Lesouhaitier O" first="Olivier" last="Lesouhaitier">Olivier Lesouhaitier</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN, Evreux, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN, Evreux</wicri:regionArea></affiliation></author></analytic><series><title level="j">Scientific reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have previously shown that the eukaryotic C-type natriuretic peptide hormone (CNP) regulates Pseudomonas aeruginosa virulence and biofilm formation after binding on the AmiC sensor, triggering the amiE transcription. Herein, the involvement of the aliphatic amidase AmiE in P. aeruginosa virulence regulation has been investigated. The proteome analysis of an AmiE over-producing strain (AmiE(+)) revealed an expression change for 138 proteins, including some that are involved in motility, synthesis of quorum sensing compounds and virulence regulation. We observed that the AmiE(+) strain produced less biofilm compared to the wild type, and over-produced rhamnolipids. In the same line, AmiE is involved in P. aeruginosa motilities (swarming and twitching) and production of the quorum sensing molecules N-acyl homoserine lactones and Pseudomonas Quinolone Signal (PQS). We observed that AmiE overproduction reduced levels of HCN and pyocyanin causing a decreased virulence in different hosts (i.e. Dictyostelium discoideum and Caenorhabditis elegans). This phenotype was further confirmed in a mouse model of acute lung infection, in which AmiE overproduction resulted in an almost fully virulence decrease. Taken together, our data suggest that, in addition to its role in bacterial secondary metabolism, AmiE is involved in P. aeruginosa virulence regulation by modulating pilus synthesis and cell-to-cell communication.</div></front></TEI><pubmed><MedlineCitation Status="In-Data-Review" Owner="NLM"><PMID Version="1">28117457</PMID><DateCreated><Year>2017</Year><Month>01</Month><Day>24</Day></DateCreated><DateRevised><Year>2017</Year><Month>02</Month><Day>20</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2045-2322</ISSN><JournalIssue CitedMedium="Internet"><Volume>7</Volume><PubDate><Year>2017</Year><Month>Jan</Month><Day>24</Day></PubDate></JournalIssue><Title>Scientific reports</Title><ISOAbbreviation>Sci Rep</ISOAbbreviation></Journal><ArticleTitle>The aliphatic amidase AmiE is involved in regulation of Pseudomonas aeruginosa virulence.</ArticleTitle><Pagination><MedlinePgn>41178</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1038/srep41178</ELocationID><Abstract><AbstractText>We have previously shown that the eukaryotic C-type natriuretic peptide hormone (CNP) regulates Pseudomonas aeruginosa virulence and biofilm formation after binding on the AmiC sensor, triggering the amiE transcription. Herein, the involvement of the aliphatic amidase AmiE in P. aeruginosa virulence regulation has been investigated. The proteome analysis of an AmiE over-producing strain (AmiE(+)) revealed an expression change for 138 proteins, including some that are involved in motility, synthesis of quorum sensing compounds and virulence regulation. We observed that the AmiE(+) strain produced less biofilm compared to the wild type, and over-produced rhamnolipids. In the same line, AmiE is involved in P. aeruginosa motilities (swarming and twitching) and production of the quorum sensing molecules N-acyl homoserine lactones and Pseudomonas Quinolone Signal (PQS). We observed that AmiE overproduction reduced levels of HCN and pyocyanin causing a decreased virulence in different hosts (i.e. Dictyostelium discoideum and Caenorhabditis elegans). This phenotype was further confirmed in a mouse model of acute lung infection, in which AmiE overproduction resulted in an almost fully virulence decrease. Taken together, our data suggest that, in addition to its role in bacterial secondary metabolism, AmiE is involved in P. aeruginosa virulence regulation by modulating pilus synthesis and cell-to-cell communication.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Clamens</LastName><ForeName>Thomas</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN, Evreux, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rosay</LastName><ForeName>Thibaut</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN, Evreux, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Crépin</LastName><ForeName>Alexandre</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Univ. Bretagne-Sud, EA 3884, LBCM, IUEM, Lorient, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Grandjean</LastName><ForeName>Teddy</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Univ. Lille, CHU Lille, EA 7366 - Recherche Translationnelle: relations hôte pathogènes, Lille, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kentache</LastName><ForeName>Takfarinas</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Laboratory « Polymères, Biopolymères, Surfaces » (UMR 6270 CNRS), Proteomic Platform PISSARO, Normandie Univ, UNIROUEN, Mont-Saint-Aignan, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hardouin</LastName><ForeName>Julie</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Laboratory « Polymères, Biopolymères, Surfaces » (UMR 6270 CNRS), Proteomic Platform PISSARO, Normandie Univ, UNIROUEN, Mont-Saint-Aignan, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bortolotti</LastName><ForeName>Perrine</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Univ. Lille, CHU Lille, EA 7366 - Recherche Translationnelle: relations hôte pathogènes, Lille, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Neidig</LastName><ForeName>Anke</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Karlsruhe Institute of Technology (KIT), Institute of Functional Interfaces, PO Box 3640, Karlsruhe, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mooij</LastName><ForeName>Marlies</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>BIOMERIT Research Centre, University College Cork, Cork, Ireland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hillion</LastName><ForeName>Mélanie</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN, Evreux, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vieillard</LastName><ForeName>Julien</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Normandie Univ, UNIROUEN, INSA Rouen, CNRS, COBRA (UMR 6014), Evreux, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cosette</LastName><ForeName>Pascal</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Laboratory « Polymères, Biopolymères, Surfaces » (UMR 6270 CNRS), Proteomic Platform PISSARO, Normandie Univ, UNIROUEN, Mont-Saint-Aignan, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Overhage</LastName><ForeName>Joerg</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Karlsruhe Institute of Technology (KIT), Institute of Functional Interfaces, PO Box 3640, Karlsruhe, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>O'Gara</LastName><ForeName>Fergal</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>BIOMERIT Research Centre, University College Cork, Cork, Ireland.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>School of Biomedical Sciences, Curtin Health Innovation Research Institute, Curtin University, Perth, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bouffartigues</LastName><ForeName>Emeline</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN, Evreux, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dufour</LastName><ForeName>Alain</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Univ. Bretagne-Sud, EA 3884, LBCM, IUEM, Lorient, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chevalier</LastName><ForeName>Sylvie</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN, Evreux, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guery</LastName><ForeName>Benoit</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Univ. Lille, CHU Lille, EA 7366 - Recherche Translationnelle: relations hôte pathogènes, Lille, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cornelis</LastName><ForeName>Pierre</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN, Evreux, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Feuilloley</LastName><ForeName>Marc G J</ForeName><Initials>MG</Initials><AffiliationInfo><Affiliation>Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN, Evreux, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lesouhaitier</LastName><ForeName>Olivier</ForeName><Initials>O</Initials><AffiliationInfo><Affiliation>Laboratory of Microbiology Signals and Microenvironment LMSM EA 4312, Normandie Univ, UNIROUEN, Evreux, 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