Effect of the sterilization method on the properties of Bombyx mori silk fibroin films.
Identifieur interne : 003B43 ( PubMed/Corpus ); précédent : 003B42; suivant : 003B44Effect of the sterilization method on the properties of Bombyx mori silk fibroin films.
Auteurs : Karina A. George ; Audra M A. Shadforth ; Traian V. Chirila ; Matthieu J. Laurent ; Sally-Anne Stephenson ; Grant A. Edwards ; Peter W. Madden ; Dietmar W. Hutmacher ; Damien G. HarkinSource :
- Materials science & engineering. C, Materials for biological applications [ 1873-0191 ] ; 2013.
English descriptors
- KwdEn :
- Animals, Biocompatible Materials (chemistry), Biocompatible Materials (pharmacology), Bombyx, Cell Adhesion (drug effects), Cell Culture Techniques (methods), Cell Line, Fibroins (chemistry), Fibroins (pharmacology), Fibroins (physiology), Humans, Sterilization, Surface Properties, Tensile Strength (physiology), Tissue Engineering.
- MESH :
- chemical , chemistry : Biocompatible Materials, Fibroins.
- chemical , pharmacology : Biocompatible Materials, Fibroins.
- drug effects : Cell Adhesion.
- methods : Cell Culture Techniques.
- chemical , physiology : Fibroins, Tensile Strength.
- Animals, Bombyx, Cell Line, Humans, Sterilization, Surface Properties, Tissue Engineering.
Abstract
We have compared the effects of different sterilization techniques on the properties of Bombyx mori silk fibroin thin films with the view to subsequent use for corneal tissue engineering. The transparency, tensile properties, corneal epithelial cell attachment and degradation of the films were used to evaluate the suitability of certain sterilization techniques including gamma-irradiation (in air or nitrogen), steam treatment and immersion in aqueous ethanol. The investigations showed that gamma-irradiation, performed either in air or in a nitrogen atmosphere, did not significantly alter the properties of films. The films sterilized by gamma-irradiation or by immersion in ethanol had a transparency greater than 98% and tensile properties comparable to human cornea and amniotic membrane, the materials of choice in the reconstruction of ocular surface. Although steam-sterilization produced stronger, stiffer films, they were less transparent, and cell attachment was affected by the variable topography of these films. It was concluded that gamma-irradiation should be considered to be the most suitable method for the sterilization of silk fibroin films, however, the treatment with ethanol is also an acceptable method.
DOI: 10.1016/j.msec.2012.10.016
PubMed: 25427472
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Shadforth</name><affiliation><nlm:affiliation>Queensland Eye Institute, 41 Annerley Road, South Brisbane, Queensland 4101, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Chirila, Traian V" sort="Chirila, Traian V" uniqKey="Chirila T" first="Traian V" last="Chirila">Traian V. Chirila</name><affiliation><nlm:affiliation>Queensland Eye Institute, 41 Annerley Road, South Brisbane, Queensland 4101, Australia; Australian Institute of Bioengineering and Nanotechnology, University of Queensland, St Lucia, 4072, Australia; Faculty of Health Sciences, University of Queensland, Herston, Queensland 4006, Australia; Faculty of Science and Engineering, Queensland University of Technology, Brisbane, Queensland 4001, Australia. Electronic address: traian.chirila@qei.org.au.</nlm:affiliation></affiliation></author><author><name sortKey="Laurent, Matthieu J" sort="Laurent, Matthieu J" uniqKey="Laurent M" first="Matthieu J" last="Laurent">Matthieu J. Laurent</name><affiliation><nlm:affiliation>Queensland Eye Institute, 41 Annerley Road, South Brisbane, Queensland 4101, Australia; Ecole Supérieure d'Ingénieurs de Luminy (ESIL), Université de la Méditerranée Aix-Marseille II, Luminy case 925 13288, Marseille, Cedex 09, France.</nlm:affiliation></affiliation></author><author><name sortKey="Stephenson, Sally Anne" sort="Stephenson, Sally Anne" uniqKey="Stephenson S" first="Sally-Anne" last="Stephenson">Sally-Anne Stephenson</name><affiliation><nlm:affiliation>Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, Queensland 4059, Australia; Faculty of Health, Queensland University of Technology, Brisbane, Queensland 4001, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Edwards, Grant A" sort="Edwards, Grant A" uniqKey="Edwards G" first="Grant A" last="Edwards">Grant A. Edwards</name><affiliation><nlm:affiliation>Australian Institute of Bioengineering and Nanotechnology, University of Queensland, St Lucia, 4072, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Madden, Peter W" sort="Madden, Peter W" uniqKey="Madden P" first="Peter W" last="Madden">Peter W. Madden</name><affiliation><nlm:affiliation>Queensland Eye Institute, 41 Annerley Road, South Brisbane, Queensland 4101, Australia; Faculty of Health Sciences, University of Queensland, Herston, Queensland 4006, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Hutmacher, Dietmar W" sort="Hutmacher, Dietmar W" uniqKey="Hutmacher D" first="Dietmar W" last="Hutmacher">Dietmar W. 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George</name><affiliation><nlm:affiliation>Queensland Eye Institute, 41 Annerley Road, South Brisbane, Queensland 4101, Australia; Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, Queensland 4059, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Shadforth, Audra M A" sort="Shadforth, Audra M A" uniqKey="Shadforth A" first="Audra M A" last="Shadforth">Audra M A. Shadforth</name><affiliation><nlm:affiliation>Queensland Eye Institute, 41 Annerley Road, South Brisbane, Queensland 4101, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Chirila, Traian V" sort="Chirila, Traian V" uniqKey="Chirila T" first="Traian V" last="Chirila">Traian V. Chirila</name><affiliation><nlm:affiliation>Queensland Eye Institute, 41 Annerley Road, South Brisbane, Queensland 4101, Australia; Australian Institute of Bioengineering and Nanotechnology, University of Queensland, St Lucia, 4072, Australia; Faculty of Health Sciences, University of Queensland, Herston, Queensland 4006, Australia; Faculty of Science and Engineering, Queensland University of Technology, Brisbane, Queensland 4001, Australia. Electronic address: traian.chirila@qei.org.au.</nlm:affiliation></affiliation></author><author><name sortKey="Laurent, Matthieu J" sort="Laurent, Matthieu J" uniqKey="Laurent M" first="Matthieu J" last="Laurent">Matthieu J. Laurent</name><affiliation><nlm:affiliation>Queensland Eye Institute, 41 Annerley Road, South Brisbane, Queensland 4101, Australia; Ecole Supérieure d'Ingénieurs de Luminy (ESIL), Université de la Méditerranée Aix-Marseille II, Luminy case 925 13288, Marseille, Cedex 09, France.</nlm:affiliation></affiliation></author><author><name sortKey="Stephenson, Sally Anne" sort="Stephenson, Sally Anne" uniqKey="Stephenson S" first="Sally-Anne" last="Stephenson">Sally-Anne Stephenson</name><affiliation><nlm:affiliation>Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, Queensland 4059, Australia; Faculty of Health, Queensland University of Technology, Brisbane, Queensland 4001, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Edwards, Grant A" sort="Edwards, Grant A" uniqKey="Edwards G" first="Grant A" last="Edwards">Grant A. Edwards</name><affiliation><nlm:affiliation>Australian Institute of Bioengineering and Nanotechnology, University of Queensland, St Lucia, 4072, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Madden, Peter W" sort="Madden, Peter W" uniqKey="Madden P" first="Peter W" last="Madden">Peter W. Madden</name><affiliation><nlm:affiliation>Queensland Eye Institute, 41 Annerley Road, South Brisbane, Queensland 4101, Australia; Faculty of Health Sciences, University of Queensland, Herston, Queensland 4006, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Hutmacher, Dietmar W" sort="Hutmacher, Dietmar W" uniqKey="Hutmacher D" first="Dietmar W" last="Hutmacher">Dietmar W. Hutmacher</name><affiliation><nlm:affiliation>Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, Queensland 4059, Australia; Faculty of Built Environment and Engineering, Queensland University of Technology, Brisbane, Queensland 4001, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Harkin, Damien G" sort="Harkin, Damien G" uniqKey="Harkin D" first="Damien G" last="Harkin">Damien G. Harkin</name><affiliation><nlm:affiliation>Queensland Eye Institute, 41 Annerley Road, South Brisbane, Queensland 4101, Australia; Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, Queensland 4059, Australia.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Materials science & engineering. C, Materials for biological applications</title><idno type="eISSN">1873-0191</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Biocompatible Materials (chemistry)</term><term>Biocompatible Materials (pharmacology)</term><term>Bombyx</term><term>Cell Adhesion (drug effects)</term><term>Cell Culture Techniques (methods)</term><term>Cell Line</term><term>Fibroins (chemistry)</term><term>Fibroins (pharmacology)</term><term>Fibroins (physiology)</term><term>Humans</term><term>Sterilization</term><term>Surface Properties</term><term>Tensile Strength (physiology)</term><term>Tissue Engineering</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Biocompatible Materials</term><term>Fibroins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Biocompatible Materials</term><term>Fibroins</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cell Adhesion</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Cell Culture Techniques</term></keywords><keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Fibroins</term><term>Tensile Strength</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Bombyx</term><term>Cell Line</term><term>Humans</term><term>Sterilization</term><term>Surface Properties</term><term>Tissue Engineering</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have compared the effects of different sterilization techniques on the properties of Bombyx mori silk fibroin thin films with the view to subsequent use for corneal tissue engineering. The transparency, tensile properties, corneal epithelial cell attachment and degradation of the films were used to evaluate the suitability of certain sterilization techniques including gamma-irradiation (in air or nitrogen), steam treatment and immersion in aqueous ethanol. The investigations showed that gamma-irradiation, performed either in air or in a nitrogen atmosphere, did not significantly alter the properties of films. The films sterilized by gamma-irradiation or by immersion in ethanol had a transparency greater than 98% and tensile properties comparable to human cornea and amniotic membrane, the materials of choice in the reconstruction of ocular surface. Although steam-sterilization produced stronger, stiffer films, they were less transparent, and cell attachment was affected by the variable topography of these films. It was concluded that gamma-irradiation should be considered to be the most suitable method for the sterilization of silk fibroin films, however, the treatment with ethanol is also an acceptable method.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25427472</PMID><DateCreated><Year>2014</Year><Month>11</Month><Day>27</Day></DateCreated><DateCompleted><Year>2015</Year><Month>07</Month><Day>15</Day></DateCompleted><DateRevised><Year>2014</Year><Month>11</Month><Day>27</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-0191</ISSN><JournalIssue CitedMedium="Internet"><Volume>33</Volume><Issue>2</Issue><PubDate><Year>2013</Year><Month>Mar</Month><Day>01</Day></PubDate></JournalIssue><Title>Materials science & engineering. C, Materials for biological applications</Title><ISOAbbreviation>Mater Sci Eng C Mater Biol Appl</ISOAbbreviation></Journal><ArticleTitle>Effect of the sterilization method on the properties of Bombyx mori silk fibroin films.</ArticleTitle><Pagination><MedlinePgn>668-74</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.msec.2012.10.016</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0928-4931(12)00479-1</ELocationID><Abstract><AbstractText>We have compared the effects of different sterilization techniques on the properties of Bombyx mori silk fibroin thin films with the view to subsequent use for corneal tissue engineering. The transparency, tensile properties, corneal epithelial cell attachment and degradation of the films were used to evaluate the suitability of certain sterilization techniques including gamma-irradiation (in air or nitrogen), steam treatment and immersion in aqueous ethanol. The investigations showed that gamma-irradiation, performed either in air or in a nitrogen atmosphere, did not significantly alter the properties of films. The films sterilized by gamma-irradiation or by immersion in ethanol had a transparency greater than 98% and tensile properties comparable to human cornea and amniotic membrane, the materials of choice in the reconstruction of ocular surface. Although steam-sterilization produced stronger, stiffer films, they were less transparent, and cell attachment was affected by the variable topography of these films. It was concluded that gamma-irradiation should be considered to be the most suitable method for the sterilization of silk fibroin films, however, the treatment with ethanol is also an acceptable method.</AbstractText><CopyrightInformation>Copyright © 2012 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>George</LastName><ForeName>Karina A</ForeName><Initials>KA</Initials><AffiliationInfo><Affiliation>Queensland Eye Institute, 41 Annerley Road, South Brisbane, Queensland 4101, Australia; Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, Queensland 4059, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shadforth</LastName><ForeName>Audra M A</ForeName><Initials>AM</Initials><AffiliationInfo><Affiliation>Queensland Eye Institute, 41 Annerley Road, South Brisbane, Queensland 4101, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chirila</LastName><ForeName>Traian V</ForeName><Initials>TV</Initials><AffiliationInfo><Affiliation>Queensland Eye Institute, 41 Annerley Road, South Brisbane, Queensland 4101, Australia; Australian Institute of Bioengineering and Nanotechnology, University of Queensland, St Lucia, 4072, Australia; Faculty of Health Sciences, University of Queensland, Herston, Queensland 4006, Australia; Faculty of Science and Engineering, Queensland University of Technology, Brisbane, Queensland 4001, Australia. Electronic address: traian.chirila@qei.org.au.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Laurent</LastName><ForeName>Matthieu J</ForeName><Initials>MJ</Initials><AffiliationInfo><Affiliation>Queensland Eye Institute, 41 Annerley Road, South Brisbane, Queensland 4101, Australia; Ecole Supérieure d'Ingénieurs de Luminy (ESIL), Université de la Méditerranée Aix-Marseille II, Luminy case 925 13288, Marseille, Cedex 09, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Stephenson</LastName><ForeName>Sally-Anne</ForeName><Initials>SA</Initials><AffiliationInfo><Affiliation>Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, Queensland 4059, Australia; Faculty of Health, Queensland University of Technology, Brisbane, Queensland 4001, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Edwards</LastName><ForeName>Grant A</ForeName><Initials>GA</Initials><AffiliationInfo><Affiliation>Australian Institute of Bioengineering and Nanotechnology, University of Queensland, St Lucia, 4072, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Madden</LastName><ForeName>Peter W</ForeName><Initials>PW</Initials><AffiliationInfo><Affiliation>Queensland Eye Institute, 41 Annerley Road, South Brisbane, Queensland 4101, Australia; Faculty of Health Sciences, University of Queensland, Herston, Queensland 4006, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hutmacher</LastName><ForeName>Dietmar W</ForeName><Initials>DW</Initials><AffiliationInfo><Affiliation>Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, Queensland 4059, Australia; Faculty of Built Environment and Engineering, Queensland University of Technology, Brisbane, Queensland 4001, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Harkin</LastName><ForeName>Damien G</ForeName><Initials>DG</Initials><AffiliationInfo><Affiliation>Queensland Eye Institute, 41 Annerley Road, South Brisbane, Queensland 4101, Australia; Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, Queensland 4059, Australia.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>11</Month><Day>02</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Mater Sci Eng C Mater Biol Appl</MedlineTA><NlmUniqueID>101484109</NlmUniqueID><ISSNLinking>0928-4931</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001672">Biocompatible Materials</NameOfSubstance></Chemical><Chemical><RegistryNumber>9007-76-5</RegistryNumber><NameOfSubstance UI="D005349">Fibroins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001672" MajorTopicYN="N">Biocompatible Materials</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012831" MajorTopicYN="Y">Bombyx</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002448" MajorTopicYN="N">Cell Adhesion</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018929" MajorTopicYN="N">Cell Culture Techniques</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005349" MajorTopicYN="N">Fibroins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013242" MajorTopicYN="N">Sterilization</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013499" MajorTopicYN="N">Surface Properties</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013718" MajorTopicYN="N">Tensile Strength</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D023822" MajorTopicYN="N">Tissue Engineering</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2012</Year><Month>05</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2012</Year><Month>10</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2012</Year><Month>10</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>11</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>3</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>7</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25427472</ArticleId><ArticleId IdType="pii">S0928-4931(12)00479-1</ArticleId><ArticleId IdType="doi">10.1016/j.msec.2012.10.016</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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