Development of microsatellite markers for the rapid and reliable genotyping of Brettanomyces bruxellensis at strain level.
Identifieur interne : 003472 ( PubMed/Corpus ); précédent : 003471; suivant : 003473Development of microsatellite markers for the rapid and reliable genotyping of Brettanomyces bruxellensis at strain level.
Auteurs : Warren Albertin ; Aurélie Panfili ; Cécile Miot-Sertier ; Aurélie Goulielmakis ; Adline Delcamp ; Franck Salin ; Aline Lonvaud-Funel ; Chris Curtin ; Isabelle Masneuf-PomaredeSource :
- Food microbiology [ 1095-9998 ] ; 2014.
English descriptors
- KwdEn :
- MESH :
- analysis : Food Contamination.
- classification : Brettanomyces.
- genetics : Brettanomyces.
- isolation & purification : Brettanomyces.
- methods : Mycological Typing Techniques.
- Alcoholic Beverages, Genotype, Microsatellite Repeats.
Abstract
Although many yeasts are useful for food production and beverage, some species may cause spoilage with important economic loss. This is the case of Dekkera/Brettanomyces bruxellensis, a contaminant species that is mainly associated with fermented beverages (wine, beer, cider and traditional drinks). To better control Brettanomyces spoilage, rapid and reliable genotyping methods are necessary to determine the origins of the spoilage, to assess the effectiveness of preventive treatments and to develop new control strategies. Despite several previously published typing methods, ranging from classical molecular methods (RAPD, AFLP, REA-PFGE, mtDNA restriction analysis) to more engineered technologies (infrared spectroscopy), there is still a lack of a rapid, reliable and universal genotyping approach. In this work, we developed eight polymorphic microsatellites markers for the Brettanomyces/Dekkera bruxellensis species. Microsatellite typing was applied to the genetic analysis of wine and beer isolates from Europe, Australia and South Africa. Our results suggest that B. bruxellensis is a highly disseminated species, with some strains isolated from different continents being closely related at the genetic level. We also focused on strains isolated from two Bordeaux wineries on different substrates (grapes, red wines) and for different vintages (over half a century). We showed that all B. bruxellensis strains within a cellar are strongly related at the genetic level, suggesting that one clonal population may cause spoilage over decades. The microsatellite tool now paves the way for future population genetics research of the B. bruxellensis species.
DOI: 10.1016/j.fm.2014.03.012
PubMed: 24929736
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pubmed:24929736Le document en format XML
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Electronic address: warren.albertin@u-bordeaux.fr.</nlm:affiliation></affiliation></author><author><name sortKey="Panfili, Aurelie" sort="Panfili, Aurelie" uniqKey="Panfili A" first="Aurélie" last="Panfili">Aurélie Panfili</name><affiliation><nlm:affiliation>Univ. de Bordeaux, ISVV, EA 4577, Unité de recherche Œnologie, F-33140 Villenave d'Ornon, France.</nlm:affiliation></affiliation></author><author><name sortKey="Miot Sertier, Cecile" sort="Miot Sertier, Cecile" uniqKey="Miot Sertier C" first="Cécile" last="Miot-Sertier">Cécile Miot-Sertier</name><affiliation><nlm:affiliation>INRA, ISVV, USC 1219 OENOLOGIE, F-33140 Villenave d'Ornon, France.</nlm:affiliation></affiliation></author><author><name sortKey="Goulielmakis, Aurelie" sort="Goulielmakis, Aurelie" uniqKey="Goulielmakis A" first="Aurélie" last="Goulielmakis">Aurélie Goulielmakis</name><affiliation><nlm:affiliation>CRB Œnologie, Centre de Ressources Biologiques Oenologique, ISVV, F-33140 Villenave d'Ornon, France.</nlm:affiliation></affiliation></author><author><name sortKey="Delcamp, Adline" sort="Delcamp, Adline" uniqKey="Delcamp A" first="Adline" last="Delcamp">Adline Delcamp</name><affiliation><nlm:affiliation>INRA, UMR Biodiversité Gènes et Ecosystèmes, PlateForme Génomique, F-33600 Cestas, France.</nlm:affiliation></affiliation></author><author><name sortKey="Salin, Franck" sort="Salin, Franck" uniqKey="Salin F" first="Franck" last="Salin">Franck Salin</name><affiliation><nlm:affiliation>INRA, UMR Biodiversité Gènes et Ecosystèmes, PlateForme Génomique, F-33600 Cestas, France.</nlm:affiliation></affiliation></author><author><name sortKey="Lonvaud Funel, Aline" sort="Lonvaud Funel, Aline" uniqKey="Lonvaud Funel A" first="Aline" last="Lonvaud-Funel">Aline Lonvaud-Funel</name><affiliation><nlm:affiliation>Univ. de Bordeaux, ISVV, EA 4577, Unité de recherche Œnologie, F-33140 Villenave d'Ornon, France.</nlm:affiliation></affiliation></author><author><name sortKey="Curtin, Chris" sort="Curtin, Chris" uniqKey="Curtin C" first="Chris" last="Curtin">Chris Curtin</name><affiliation><nlm:affiliation>The Australian Wine Research Institute, Glen Osmond, Adelaide, SA, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Masneuf Pomarede, Isabelle" sort="Masneuf Pomarede, Isabelle" uniqKey="Masneuf Pomarede I" first="Isabelle" last="Masneuf-Pomarede">Isabelle Masneuf-Pomarede</name><affiliation><nlm:affiliation>Univ. de Bordeaux, ISVV, EA 4577, Unité de recherche Œnologie, F-33140 Villenave d'Ornon, France; Bordeaux Sciences Agro, F-33175 Gradignan, France. Electronic address: isabelle.masneuf@agro-bordeaux.fr.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Food microbiology</title><idno type="eISSN">1095-9998</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alcoholic Beverages</term><term>Brettanomyces (classification)</term><term>Brettanomyces (genetics)</term><term>Brettanomyces (isolation & purification)</term><term>Food Contamination (analysis)</term><term>Genotype</term><term>Microsatellite Repeats</term><term>Mycological Typing Techniques (methods)</term></keywords><keywords scheme="MESH" qualifier="analysis" xml:lang="en"><term>Food Contamination</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Brettanomyces</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Brettanomyces</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Brettanomyces</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Mycological Typing Techniques</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Alcoholic Beverages</term><term>Genotype</term><term>Microsatellite Repeats</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Although many yeasts are useful for food production and beverage, some species may cause spoilage with important economic loss. This is the case of Dekkera/Brettanomyces bruxellensis, a contaminant species that is mainly associated with fermented beverages (wine, beer, cider and traditional drinks). To better control Brettanomyces spoilage, rapid and reliable genotyping methods are necessary to determine the origins of the spoilage, to assess the effectiveness of preventive treatments and to develop new control strategies. Despite several previously published typing methods, ranging from classical molecular methods (RAPD, AFLP, REA-PFGE, mtDNA restriction analysis) to more engineered technologies (infrared spectroscopy), there is still a lack of a rapid, reliable and universal genotyping approach. In this work, we developed eight polymorphic microsatellites markers for the Brettanomyces/Dekkera bruxellensis species. Microsatellite typing was applied to the genetic analysis of wine and beer isolates from Europe, Australia and South Africa. Our results suggest that B. bruxellensis is a highly disseminated species, with some strains isolated from different continents being closely related at the genetic level. We also focused on strains isolated from two Bordeaux wineries on different substrates (grapes, red wines) and for different vintages (over half a century). We showed that all B. bruxellensis strains within a cellar are strongly related at the genetic level, suggesting that one clonal population may cause spoilage over decades. The microsatellite tool now paves the way for future population genetics research of the B. bruxellensis species.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24929736</PMID><DateCreated><Year>2014</Year><Month>06</Month><Day>16</Day></DateCreated><DateCompleted><Year>2015</Year><Month>01</Month><Day>26</Day></DateCompleted><DateRevised><Year>2014</Year><Month>06</Month><Day>16</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1095-9998</ISSN><JournalIssue CitedMedium="Internet"><Volume>42</Volume><PubDate><Year>2014</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Food microbiology</Title><ISOAbbreviation>Food Microbiol.</ISOAbbreviation></Journal><ArticleTitle>Development of microsatellite markers for the rapid and reliable genotyping of Brettanomyces bruxellensis at strain level.</ArticleTitle><Pagination><MedlinePgn>188-95</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.fm.2014.03.012</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0740-0020(14)00065-3</ELocationID><Abstract><AbstractText>Although many yeasts are useful for food production and beverage, some species may cause spoilage with important economic loss. This is the case of Dekkera/Brettanomyces bruxellensis, a contaminant species that is mainly associated with fermented beverages (wine, beer, cider and traditional drinks). To better control Brettanomyces spoilage, rapid and reliable genotyping methods are necessary to determine the origins of the spoilage, to assess the effectiveness of preventive treatments and to develop new control strategies. Despite several previously published typing methods, ranging from classical molecular methods (RAPD, AFLP, REA-PFGE, mtDNA restriction analysis) to more engineered technologies (infrared spectroscopy), there is still a lack of a rapid, reliable and universal genotyping approach. In this work, we developed eight polymorphic microsatellites markers for the Brettanomyces/Dekkera bruxellensis species. Microsatellite typing was applied to the genetic analysis of wine and beer isolates from Europe, Australia and South Africa. Our results suggest that B. bruxellensis is a highly disseminated species, with some strains isolated from different continents being closely related at the genetic level. We also focused on strains isolated from two Bordeaux wineries on different substrates (grapes, red wines) and for different vintages (over half a century). We showed that all B. bruxellensis strains within a cellar are strongly related at the genetic level, suggesting that one clonal population may cause spoilage over decades. The microsatellite tool now paves the way for future population genetics research of the B. bruxellensis species.</AbstractText><CopyrightInformation>Copyright © 2014 Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Albertin</LastName><ForeName>Warren</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Univ. de Bordeaux, ISVV, EA 4577, Unité de recherche Œnologie, F-33140 Villenave d'Ornon, France; Bordeaux Sciences Agro, F-33175 Gradignan, France. Electronic address: warren.albertin@u-bordeaux.fr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Panfili</LastName><ForeName>Aurélie</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Univ. de Bordeaux, ISVV, EA 4577, Unité de recherche Œnologie, F-33140 Villenave d'Ornon, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Miot-Sertier</LastName><ForeName>Cécile</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>INRA, ISVV, USC 1219 OENOLOGIE, F-33140 Villenave d'Ornon, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Goulielmakis</LastName><ForeName>Aurélie</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>CRB Œnologie, Centre de Ressources Biologiques Oenologique, ISVV, F-33140 Villenave d'Ornon, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Delcamp</LastName><ForeName>Adline</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>INRA, UMR Biodiversité Gènes et Ecosystèmes, PlateForme Génomique, F-33600 Cestas, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Salin</LastName><ForeName>Franck</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>INRA, UMR Biodiversité Gènes et Ecosystèmes, PlateForme Génomique, F-33600 Cestas, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lonvaud-Funel</LastName><ForeName>Aline</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Univ. de Bordeaux, ISVV, EA 4577, Unité de recherche Œnologie, F-33140 Villenave d'Ornon, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Curtin</LastName><ForeName>Chris</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>The Australian Wine Research Institute, Glen Osmond, Adelaide, SA, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Masneuf-Pomarede</LastName><ForeName>Isabelle</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>Univ. de Bordeaux, ISVV, EA 4577, Unité de recherche Œnologie, F-33140 Villenave d'Ornon, France; Bordeaux Sciences Agro, F-33175 Gradignan, France. Electronic address: isabelle.masneuf@agro-bordeaux.fr.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D023362">Evaluation Studies</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>03</Month><Day>24</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Food Microbiol</MedlineTA><NlmUniqueID>8601127</NlmUniqueID><ISSNLinking>0740-0020</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000434" MajorTopicYN="N">Alcoholic Beverages</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055168" MajorTopicYN="N">Brettanomyces</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005506" MajorTopicYN="N">Food Contamination</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005838" MajorTopicYN="N">Genotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018895" MajorTopicYN="Y">Microsatellite Repeats</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016533" MajorTopicYN="N">Mycological Typing Techniques</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">SSR</Keyword><Keyword MajorTopicYN="N">STR</Keyword><Keyword MajorTopicYN="N">Spoilage yeast</Keyword><Keyword MajorTopicYN="N">VNTR</Keyword><Keyword MajorTopicYN="N">Wine</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2013</Year><Month>10</Month><Day>01</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2014</Year><Month>02</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2014</Year><Month>03</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>6</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>6</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>1</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24929736</ArticleId><ArticleId IdType="pii">S0740-0020(14)00065-3</ArticleId><ArticleId IdType="doi">10.1016/j.fm.2014.03.012</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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