Variability within a pea core collection of LEAM and HSP22, two mitochondrial seed proteins involved in stress tolerance.
Identifieur interne : 002B81 ( PubMed/Corpus ); précédent : 002B80; suivant : 002B82Variability within a pea core collection of LEAM and HSP22, two mitochondrial seed proteins involved in stress tolerance.
Auteurs : Marie-Hélène Avelange-Macherel ; Nicole Payet ; David Lalanne ; Martine Neveu ; Dimitri Tolleter ; Judith Burstin ; David MacherelSource :
- Plant, cell & environment [ 1365-3040 ] ; 2015.
English descriptors
- KwdEn :
- Amino Acid Sequence, Genotype, Heat-Shock Proteins (genetics), Heat-Shock Proteins (metabolism), Mitochondria (metabolism), Mitochondrial Proteins (genetics), Mitochondrial Proteins (metabolism), Peas (genetics), Peas (growth & development), Peas (physiology), Plant Proteins (genetics), Plant Proteins (metabolism), Protein Isoforms, Seeds (metabolism), Sequence Alignment, Stress, Physiological, Temperature.
- MESH :
- chemical , genetics : Heat-Shock Proteins, Mitochondrial Proteins, Plant Proteins.
- chemical , metabolism : Heat-Shock Proteins, Mitochondrial Proteins, Plant Proteins.
- genetics : Peas.
- growth & development : Peas.
- metabolism : Mitochondria, Seeds.
- physiology : Peas.
- Amino Acid Sequence, Genotype, Protein Isoforms, Sequence Alignment, Stress, Physiological, Temperature.
Abstract
LEAM, a late embryogenesis abundant protein, and HSP22, a small heat shock protein, were shown to accumulate in the mitochondria during pea (Pisum sativum L.) seed development, where they are expected to contribute to desiccation tolerance. Here, their expression was examined in seeds of 89 pea genotypes by Western blot analysis. All genotypes expressed LEAM and HSP22 in similar amounts. In contrast with HSP22, LEAM displayed different isoforms according to apparent molecular mass. Each of the 89 genotypes harboured a single LEAM isoform. Genomic and RT-PCR analysis revealed four LEAM genes differing by a small variable indel in the coding region. These variations were consistent with the apparent molecular mass of each isoform. Indels, which occurred in repeated domains, did not alter the main properties of LEAM. Structural modelling indicated that the class A α-helix structure, which allows interactions with the mitochondrial inner membrane in the dry state, was preserved in all isoforms, suggesting functionality is maintained. The overall results point out the essential character of LEAM and HSP22 in pea seeds. LEAM variability is discussed in terms of pea breeding history as well as LEA gene evolution mechanisms.
DOI: 10.1111/pce.12480
PubMed: 25367071
Links to Exploration step
pubmed:25367071Le document en format XML
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Macherel, Marie Helene" uniqKey="Avelange Macherel M" first="Marie-Hélène" last="Avelange-Macherel">Marie-Hélène Avelange-Macherel</name><affiliation><nlm:affiliation>Agrocampus Ouest, UMR 1345 Institut de Recherche en Horticulture et Semences, Angers, F-49045, France.</nlm:affiliation></affiliation></author><author><name sortKey="Payet, Nicole" sort="Payet, Nicole" uniqKey="Payet N" first="Nicole" last="Payet">Nicole Payet</name><affiliation><nlm:affiliation>INRA, UMR 1345 Institut de Recherche en Horticulture et Semences, Angers, F-49045, France.</nlm:affiliation></affiliation></author><author><name sortKey="Lalanne, David" sort="Lalanne, David" uniqKey="Lalanne D" first="David" last="Lalanne">David Lalanne</name><affiliation><nlm:affiliation>INRA, UMR 1345 Institut de Recherche en Horticulture et Semences, Angers, F-49045, France.</nlm:affiliation></affiliation></author><author><name sortKey="Neveu, Martine" sort="Neveu, Martine" uniqKey="Neveu M" first="Martine" last="Neveu">Martine Neveu</name><affiliation><nlm:affiliation>INRA, UMR 1345 Institut de Recherche en Horticulture et Semences, Angers, F-49045, France.</nlm:affiliation></affiliation></author><author><name sortKey="Tolleter, Dimitri" sort="Tolleter, Dimitri" uniqKey="Tolleter D" first="Dimitri" last="Tolleter">Dimitri Tolleter</name><affiliation><nlm:affiliation>ANU College of Medicine, Biology and Environment, Acton, 2601, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Burstin, Judith" sort="Burstin, Judith" uniqKey="Burstin J" first="Judith" last="Burstin">Judith Burstin</name><affiliation><nlm:affiliation>GEAPSI, INRA, UMR 1347 Agroécologie, centre de Dijon, F-21065, France.</nlm:affiliation></affiliation></author><author><name sortKey="Macherel, David" sort="Macherel, David" uniqKey="Macherel D" first="David" last="Macherel">David Macherel</name><affiliation><nlm:affiliation>Université d'Angers, UMR 1345 Institut de Recherche en Horticulture et Semences, Angers, F-49045, France.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Plant, cell & environment</title><idno type="eISSN">1365-3040</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Genotype</term><term>Heat-Shock Proteins (genetics)</term><term>Heat-Shock Proteins (metabolism)</term><term>Mitochondria (metabolism)</term><term>Mitochondrial Proteins (genetics)</term><term>Mitochondrial Proteins (metabolism)</term><term>Peas (genetics)</term><term>Peas (growth & development)</term><term>Peas (physiology)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Protein Isoforms</term><term>Seeds (metabolism)</term><term>Sequence Alignment</term><term>Stress, Physiological</term><term>Temperature</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Heat-Shock Proteins</term><term>Mitochondrial Proteins</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Heat-Shock Proteins</term><term>Mitochondrial Proteins</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Peas</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Peas</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Mitochondria</term><term>Seeds</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Peas</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Genotype</term><term>Protein Isoforms</term><term>Sequence Alignment</term><term>Stress, Physiological</term><term>Temperature</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">LEAM, a late embryogenesis abundant protein, and HSP22, a small heat shock protein, were shown to accumulate in the mitochondria during pea (Pisum sativum L.) seed development, where they are expected to contribute to desiccation tolerance. Here, their expression was examined in seeds of 89 pea genotypes by Western blot analysis. All genotypes expressed LEAM and HSP22 in similar amounts. In contrast with HSP22, LEAM displayed different isoforms according to apparent molecular mass. Each of the 89 genotypes harboured a single LEAM isoform. Genomic and RT-PCR analysis revealed four LEAM genes differing by a small variable indel in the coding region. These variations were consistent with the apparent molecular mass of each isoform. Indels, which occurred in repeated domains, did not alter the main properties of LEAM. Structural modelling indicated that the class A α-helix structure, which allows interactions with the mitochondrial inner membrane in the dry state, was preserved in all isoforms, suggesting functionality is maintained. The overall results point out the essential character of LEAM and HSP22 in pea seeds. LEAM variability is discussed in terms of pea breeding history as well as LEA gene evolution mechanisms.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25367071</PMID><DateCreated><Year>2015</Year><Month>06</Month><Day>04</Day></DateCreated><DateCompleted><Year>2016</Year><Month>05</Month><Day>31</Day></DateCompleted><DateRevised><Year>2015</Year><Month>06</Month><Day>04</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1365-3040</ISSN><JournalIssue CitedMedium="Internet"><Volume>38</Volume><Issue>7</Issue><PubDate><Year>2015</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Plant, cell & environment</Title><ISOAbbreviation>Plant Cell Environ.</ISOAbbreviation></Journal><ArticleTitle>Variability within a pea core collection of LEAM and HSP22, two mitochondrial seed proteins involved in stress tolerance.</ArticleTitle><Pagination><MedlinePgn>1299-311</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/pce.12480</ELocationID><Abstract><AbstractText>LEAM, a late embryogenesis abundant protein, and HSP22, a small heat shock protein, were shown to accumulate in the mitochondria during pea (Pisum sativum L.) seed development, where they are expected to contribute to desiccation tolerance. Here, their expression was examined in seeds of 89 pea genotypes by Western blot analysis. All genotypes expressed LEAM and HSP22 in similar amounts. In contrast with HSP22, LEAM displayed different isoforms according to apparent molecular mass. Each of the 89 genotypes harboured a single LEAM isoform. Genomic and RT-PCR analysis revealed four LEAM genes differing by a small variable indel in the coding region. These variations were consistent with the apparent molecular mass of each isoform. Indels, which occurred in repeated domains, did not alter the main properties of LEAM. Structural modelling indicated that the class A α-helix structure, which allows interactions with the mitochondrial inner membrane in the dry state, was preserved in all isoforms, suggesting functionality is maintained. The overall results point out the essential character of LEAM and HSP22 in pea seeds. LEAM variability is discussed in terms of pea breeding history as well as LEA gene evolution mechanisms.</AbstractText><CopyrightInformation>© 2014 John Wiley & Sons Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Avelange-Macherel</LastName><ForeName>Marie-Hélène</ForeName><Initials>MH</Initials><Identifier Source="ORCID">http://orcid.org/0000-0001-6002-3130</Identifier><AffiliationInfo><Affiliation>Agrocampus Ouest, UMR 1345 Institut de Recherche en Horticulture et Semences, Angers, F-49045, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Payet</LastName><ForeName>Nicole</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>INRA, UMR 1345 Institut de Recherche en Horticulture et Semences, Angers, F-49045, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lalanne</LastName><ForeName>David</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>INRA, UMR 1345 Institut de Recherche en Horticulture et Semences, Angers, F-49045, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Neveu</LastName><ForeName>Martine</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>INRA, UMR 1345 Institut de Recherche en Horticulture et Semences, Angers, F-49045, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tolleter</LastName><ForeName>Dimitri</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>ANU College of Medicine, Biology and Environment, Acton, 2601, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Burstin</LastName><ForeName>Judith</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>GEAPSI, INRA, UMR 1347 Agroécologie, centre de Dijon, F-21065, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Macherel</LastName><ForeName>David</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Université d'Angers, UMR 1345 Institut de Recherche en Horticulture et Semences, Angers, F-49045, France.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>12</Month><Day>26</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plant Cell Environ</MedlineTA><NlmUniqueID>9309004</NlmUniqueID><ISSNLinking>0140-7791</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C096801">HSP22 protein, plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D006360">Heat-Shock Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D024101">Mitochondrial Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020033">Protein Isoforms</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005838" MajorTopicYN="N">Genotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006360" MajorTopicYN="N">Heat-Shock Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008928" MajorTopicYN="N">Mitochondria</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D024101" MajorTopicYN="N">Mitochondrial Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018532" MajorTopicYN="N">Peas</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020033" MajorTopicYN="N">Protein Isoforms</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012639" MajorTopicYN="N">Seeds</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016415" MajorTopicYN="N">Sequence Alignment</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013312" MajorTopicYN="N">Stress, Physiological</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013696" MajorTopicYN="N">Temperature</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">LEA protein</Keyword><Keyword MajorTopicYN="N">gene evolution</Keyword><Keyword MajorTopicYN="N">indel</Keyword><Keyword MajorTopicYN="N">mitochondria</Keyword><Keyword MajorTopicYN="N">pea accessions</Keyword><Keyword MajorTopicYN="N">small HSP</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year><Month>09</Month><Day>01</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2014</Year><Month>10</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2014</Year><Month>10</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>11</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>11</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>6</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25367071</ArticleId><ArticleId IdType="doi">10.1111/pce.12480</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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