Motility of liquid stored ram spermatozoa is altered by dilution rate independent of seminal plasma concentration.
Identifieur interne : 002722 ( PubMed/Corpus ); précédent : 002721; suivant : 002723Motility of liquid stored ram spermatozoa is altered by dilution rate independent of seminal plasma concentration.
Auteurs : M. Mata-Campuzano ; C. Soleilhavoup ; G. Tsikis ; F. Martinez-Pastor ; S P De Graaf ; X. DruartSource :
- Animal reproduction science [ 1873-2232 ] ; 2015.
English descriptors
- KwdEn :
- MESH :
- methods : Semen Preservation.
- physiology : Semen, Spermatozoa.
- veterinary : Semen Preservation.
- Animals, Male, Sheep, Sperm Motility.
Abstract
The fertility after use of liquid stored ram semen following cervical AI rapidly decreases if semen is stored beyond 12h. The dilution of seminal plasma is often cited as a key contributor to the diminished motility and fertility of ram spermatozoa subjected to liquid preservation. Two experiments were conducted to assess the effect of spermatozoa concentration (i.e. dilution rate) and percentage of seminal plasma on the motility and viability of liquid stored ram spermatozoa. In Experiment 1, semen was diluted to one of seven concentrations ranging from 0.2 to 1.4×10(9)spermatozoa/ml with milk and assessed for motility after 3 or 24h of storage at 15°C. In Experiment 2, semen was collected and washed to remove seminal plasma before re-dilution to 0.2-1.4×10(9)spermatozoa/ml with milk containing 0%, 20% or 40% (final v/v ratio) seminal plasma and assessed for viability and motility after 3 or 24h of storage at 15°C. Whereas motility was not affected by spermatozoa concentration after 3h of storage, the proportion of progressive spermatozoa decreased after 24h of storage when spermatozoa concentration was greater than 1.0×10(9)spermatozoa/ml. The duration of preservation and the spermatozoa concentration affected spermatozoa motility but had no impact on spermatozoa viability. This negative effect of greater spermatozoa concentrations on motility was independent of the presence and the concentration of seminal plasma. The seminal plasma at both concentrations (20% and 40%) had a protective effect on spermatozoa motility after 24h of storage. These findings have the potential to improve the efficiency of cervical AI with liquid stored ram semen.
DOI: 10.1016/j.anireprosci.2015.09.004
PubMed: 26421370
Links to Exploration step
pubmed:26421370Le document en format XML
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Tsikis</name><affiliation><nlm:affiliation>INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; CNRS, UMR 7247, F-37380 Nouzilly, France; Université François Rabelais de Tours, F-37000 Tours, France; IFCE, F-37380 Nouzilly, France.</nlm:affiliation></affiliation></author><author><name sortKey="Martinez Pastor, F" sort="Martinez Pastor, F" uniqKey="Martinez Pastor F" first="F" last="Martinez-Pastor">F. Martinez-Pastor</name><affiliation><nlm:affiliation>INDEGSAL, University of León, 24071 León, Spain.</nlm:affiliation></affiliation></author><author><name sortKey="De Graaf, S P" sort="De Graaf, S P" uniqKey="De Graaf S" first="S P" last="De Graaf">S P De Graaf</name><affiliation><nlm:affiliation>Faculty of Veterinary Science, The University of Sydney, NSW 2006, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Druart, X" sort="Druart, X" uniqKey="Druart X" first="X" last="Druart">X. Druart</name><affiliation><nlm:affiliation>INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; CNRS, UMR 7247, F-37380 Nouzilly, France; Université François Rabelais de Tours, F-37000 Tours, France; IFCE, F-37380 Nouzilly, France. 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Mata-Campuzano</name><affiliation><nlm:affiliation>INDEGSAL, University of León, 24071 León, Spain.</nlm:affiliation></affiliation></author><author><name sortKey="Soleilhavoup, C" sort="Soleilhavoup, C" uniqKey="Soleilhavoup C" first="C" last="Soleilhavoup">C. Soleilhavoup</name><affiliation><nlm:affiliation>INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; CNRS, UMR 7247, F-37380 Nouzilly, France; Université François Rabelais de Tours, F-37000 Tours, France; IFCE, F-37380 Nouzilly, France.</nlm:affiliation></affiliation></author><author><name sortKey="Tsikis, G" sort="Tsikis, G" uniqKey="Tsikis G" first="G" last="Tsikis">G. Tsikis</name><affiliation><nlm:affiliation>INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; CNRS, UMR 7247, F-37380 Nouzilly, France; Université François Rabelais de Tours, F-37000 Tours, France; IFCE, F-37380 Nouzilly, France.</nlm:affiliation></affiliation></author><author><name sortKey="Martinez Pastor, F" sort="Martinez Pastor, F" uniqKey="Martinez Pastor F" first="F" last="Martinez-Pastor">F. Martinez-Pastor</name><affiliation><nlm:affiliation>INDEGSAL, University of León, 24071 León, Spain.</nlm:affiliation></affiliation></author><author><name sortKey="De Graaf, S P" sort="De Graaf, S P" uniqKey="De Graaf S" first="S P" last="De Graaf">S P De Graaf</name><affiliation><nlm:affiliation>Faculty of Veterinary Science, The University of Sydney, NSW 2006, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Druart, X" sort="Druart, X" uniqKey="Druart X" first="X" last="Druart">X. Druart</name><affiliation><nlm:affiliation>INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; CNRS, UMR 7247, F-37380 Nouzilly, France; Université François Rabelais de Tours, F-37000 Tours, France; IFCE, F-37380 Nouzilly, France. Electronic address: xavier.druart@tours.inra.fr.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Animal reproduction science</title><idno type="eISSN">1873-2232</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Male</term><term>Semen (physiology)</term><term>Semen Preservation (methods)</term><term>Semen Preservation (veterinary)</term><term>Sheep</term><term>Sperm Motility</term><term>Spermatozoa (physiology)</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Semen Preservation</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Semen</term><term>Spermatozoa</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Semen Preservation</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Male</term><term>Sheep</term><term>Sperm Motility</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The fertility after use of liquid stored ram semen following cervical AI rapidly decreases if semen is stored beyond 12h. The dilution of seminal plasma is often cited as a key contributor to the diminished motility and fertility of ram spermatozoa subjected to liquid preservation. Two experiments were conducted to assess the effect of spermatozoa concentration (i.e. dilution rate) and percentage of seminal plasma on the motility and viability of liquid stored ram spermatozoa. In Experiment 1, semen was diluted to one of seven concentrations ranging from 0.2 to 1.4×10(9)spermatozoa/ml with milk and assessed for motility after 3 or 24h of storage at 15°C. In Experiment 2, semen was collected and washed to remove seminal plasma before re-dilution to 0.2-1.4×10(9)spermatozoa/ml with milk containing 0%, 20% or 40% (final v/v ratio) seminal plasma and assessed for viability and motility after 3 or 24h of storage at 15°C. Whereas motility was not affected by spermatozoa concentration after 3h of storage, the proportion of progressive spermatozoa decreased after 24h of storage when spermatozoa concentration was greater than 1.0×10(9)spermatozoa/ml. The duration of preservation and the spermatozoa concentration affected spermatozoa motility but had no impact on spermatozoa viability. This negative effect of greater spermatozoa concentrations on motility was independent of the presence and the concentration of seminal plasma. The seminal plasma at both concentrations (20% and 40%) had a protective effect on spermatozoa motility after 24h of storage. These findings have the potential to improve the efficiency of cervical AI with liquid stored ram semen.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26421370</PMID><DateCreated><Year>2015</Year><Month>10</Month><Day>21</Day></DateCreated><DateCompleted><Year>2016</Year><Month>08</Month><Day>23</Day></DateCompleted><DateRevised><Year>2015</Year><Month>10</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-2232</ISSN><JournalIssue CitedMedium="Internet"><Volume>162</Volume><PubDate><Year>2015</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Animal reproduction science</Title><ISOAbbreviation>Anim. Reprod. Sci.</ISOAbbreviation></Journal><ArticleTitle>Motility of liquid stored ram spermatozoa is altered by dilution rate independent of seminal plasma concentration.</ArticleTitle><Pagination><MedlinePgn>31-6</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.anireprosci.2015.09.004</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0378-4320(15)30015-4</ELocationID><Abstract><AbstractText>The fertility after use of liquid stored ram semen following cervical AI rapidly decreases if semen is stored beyond 12h. The dilution of seminal plasma is often cited as a key contributor to the diminished motility and fertility of ram spermatozoa subjected to liquid preservation. Two experiments were conducted to assess the effect of spermatozoa concentration (i.e. dilution rate) and percentage of seminal plasma on the motility and viability of liquid stored ram spermatozoa. In Experiment 1, semen was diluted to one of seven concentrations ranging from 0.2 to 1.4×10(9)spermatozoa/ml with milk and assessed for motility after 3 or 24h of storage at 15°C. In Experiment 2, semen was collected and washed to remove seminal plasma before re-dilution to 0.2-1.4×10(9)spermatozoa/ml with milk containing 0%, 20% or 40% (final v/v ratio) seminal plasma and assessed for viability and motility after 3 or 24h of storage at 15°C. Whereas motility was not affected by spermatozoa concentration after 3h of storage, the proportion of progressive spermatozoa decreased after 24h of storage when spermatozoa concentration was greater than 1.0×10(9)spermatozoa/ml. The duration of preservation and the spermatozoa concentration affected spermatozoa motility but had no impact on spermatozoa viability. This negative effect of greater spermatozoa concentrations on motility was independent of the presence and the concentration of seminal plasma. The seminal plasma at both concentrations (20% and 40%) had a protective effect on spermatozoa motility after 24h of storage. These findings have the potential to improve the efficiency of cervical AI with liquid stored ram semen.</AbstractText><CopyrightInformation>Copyright © 2015 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Mata-Campuzano</LastName><ForeName>M</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>INDEGSAL, University of León, 24071 León, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Soleilhavoup</LastName><ForeName>C</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; CNRS, UMR 7247, F-37380 Nouzilly, France; Université François Rabelais de Tours, F-37000 Tours, France; IFCE, F-37380 Nouzilly, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tsikis</LastName><ForeName>G</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; CNRS, UMR 7247, F-37380 Nouzilly, France; Université François Rabelais de Tours, F-37000 Tours, France; IFCE, F-37380 Nouzilly, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Martinez-Pastor</LastName><ForeName>F</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>INDEGSAL, University of León, 24071 León, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>de Graaf</LastName><ForeName>S P</ForeName><Initials>SP</Initials><AffiliationInfo><Affiliation>Faculty of Veterinary Science, The University of Sydney, NSW 2006, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Druart</LastName><ForeName>X</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; CNRS, UMR 7247, F-37380 Nouzilly, France; Université François Rabelais de Tours, F-37000 Tours, France; IFCE, F-37380 Nouzilly, France. Electronic address: xavier.druart@tours.inra.fr.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>09</Month><Day>11</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Anim Reprod Sci</MedlineTA><NlmUniqueID>7807205</NlmUniqueID><ISSNLinking>0378-4320</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012661" MajorTopicYN="Y">Semen</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012662" MajorTopicYN="N">Semen Preservation</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName><QualifierName UI="Q000662" MajorTopicYN="Y">veterinary</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012756" MajorTopicYN="N">Sheep</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013081" MajorTopicYN="Y">Sperm Motility</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013094" MajorTopicYN="N">Spermatozoa</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">AI</Keyword><Keyword MajorTopicYN="N">Preservation</Keyword><Keyword MajorTopicYN="N">Ram semen</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>06</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2015</Year><Month>09</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>09</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>10</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>10</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>8</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26421370</ArticleId><ArticleId IdType="pii">S0378-4320(15)30015-4</ArticleId><ArticleId IdType="doi">10.1016/j.anireprosci.2015.09.004</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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