Automated Lab-on-a-Chip Technology for Fish Embryo Toxicity Tests Performed under Continuous Microperfusion (μFET).
Identifieur interne : 002614 ( PubMed/Corpus ); précédent : 002613; suivant : 002615Automated Lab-on-a-Chip Technology for Fish Embryo Toxicity Tests Performed under Continuous Microperfusion (μFET).
Auteurs : Feng Zhu ; Adriana Wigh ; Timo Friedrich ; Alain Devaux ; Sylvie Bony ; Dayanthi Nugegoda ; Jan Kaslin ; Donald WlodkowicSource :
- Environmental science & technology [ 1520-5851 ] ; 2015.
English descriptors
- KwdEn :
- Animals, Caffeine (toxicity), Copper Sulfate (toxicity), Dimethyl Sulfoxide (toxicity), Embryo, Nonmammalian (drug effects), Ethanol (toxicity), Lab-On-A-Chip Devices, Microfluidics (instrumentation), Microfluidics (methods), Nicotine (toxicity), Printing, Three-Dimensional, Toxicity Tests (instrumentation), Toxicity Tests (methods), Zebrafish (embryology).
- MESH :
- chemical , toxicity : Caffeine, Copper Sulfate, Dimethyl Sulfoxide, Ethanol, Nicotine.
- drug effects : Embryo, Nonmammalian.
- embryology : Zebrafish.
- instrumentation : Microfluidics, Toxicity Tests.
- methods : Microfluidics, Toxicity Tests.
- Animals, Lab-On-A-Chip Devices, Printing, Three-Dimensional.
Abstract
The fish embryo toxicity (FET) biotest has gained popularity as one of the alternative approaches to acute fish toxicity tests in chemical hazard and risk assessment. Despite the importance and common acceptance of FET, it is still performed in multiwell plates and requires laborious and time-consuming manual manipulation of specimens and solutions. This work describes the design and validation of a microfluidic Lab-on-a-Chip technology for automation of the zebrafish embryo toxicity test common in aquatic ecotoxicology. The innovative device supports rapid loading and immobilization of large numbers of zebrafish embryos suspended in a continuous microfluidic perfusion as a means of toxicant delivery. Furthermore, we also present development of a customized mechatronic automation interface that includes a high-resolution USB microscope, LED cold light illumination, and miniaturized 3D printed pumping manifolds that were integrated to enable time-resolved in situ analysis of developing fish embryos. To investigate the applicability of the microfluidic FET (μFET) in toxicity testing, copper sulfate, phenol, ethanol, caffeine, nicotine, and dimethyl sulfoxide were tested as model chemical stressors. Results obtained on a chip-based system were compared with static protocols performed in microtiter plates. This work provides evidence that FET analysis performed under microperfusion opens a brand new alternative for inexpensive automation in aquatic ecotoxicology.
DOI: 10.1021/acs.est.5b03838
PubMed: 26506399
Links to Exploration step
pubmed:26506399Le document en format XML
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Devaux</name><affiliation><nlm:affiliation>Université de Lyon, UMR LEHNA 5023, USC INRA, ENTPE, rue Maurice Audin, Vaulx-en-Velin F-69518, France.</nlm:affiliation></affiliation></author><author><name sortKey="Bony, Sylvie" sort="Bony, Sylvie" uniqKey="Bony S" first="Sylvie" last="Bony">Sylvie Bony</name><affiliation><nlm:affiliation>Université de Lyon, UMR LEHNA 5023, USC INRA, ENTPE, rue Maurice Audin, Vaulx-en-Velin F-69518, France.</nlm:affiliation></affiliation></author><author><name sortKey="Nugegoda, Dayanthi" sort="Nugegoda, Dayanthi" uniqKey="Nugegoda D" first="Dayanthi" last="Nugegoda">Dayanthi Nugegoda</name><affiliation><nlm:affiliation>Ecotoxicology Research Group, School of Applied Sciences, RMIT University , Bowen Street, Melbourne, VIC 3001, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Kaslin, Jan" sort="Kaslin, Jan" uniqKey="Kaslin J" first="Jan" last="Kaslin">Jan Kaslin</name><affiliation><nlm:affiliation>ARMI, Monash University , 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sortKey="Zhu, Feng" sort="Zhu, Feng" uniqKey="Zhu F" first="Feng" last="Zhu">Feng Zhu</name></author><author><name sortKey="Wigh, Adriana" sort="Wigh, Adriana" uniqKey="Wigh A" first="Adriana" last="Wigh">Adriana Wigh</name><affiliation><nlm:affiliation>Université de Lyon, UMR LEHNA 5023, USC INRA, ENTPE, rue Maurice Audin, Vaulx-en-Velin F-69518, France.</nlm:affiliation></affiliation></author><author><name sortKey="Friedrich, Timo" sort="Friedrich, Timo" uniqKey="Friedrich T" first="Timo" last="Friedrich">Timo Friedrich</name><affiliation><nlm:affiliation>ARMI, Monash University , Wellington Road, Clayton, VIC 3800, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Devaux, Alain" sort="Devaux, Alain" uniqKey="Devaux A" first="Alain" last="Devaux">Alain Devaux</name><affiliation><nlm:affiliation>Université de Lyon, UMR LEHNA 5023, USC INRA, ENTPE, rue Maurice Audin, Vaulx-en-Velin F-69518, France.</nlm:affiliation></affiliation></author><author><name sortKey="Bony, Sylvie" sort="Bony, Sylvie" uniqKey="Bony S" first="Sylvie" last="Bony">Sylvie Bony</name><affiliation><nlm:affiliation>Université de Lyon, UMR LEHNA 5023, USC INRA, ENTPE, rue Maurice Audin, Vaulx-en-Velin F-69518, France.</nlm:affiliation></affiliation></author><author><name sortKey="Nugegoda, Dayanthi" sort="Nugegoda, Dayanthi" uniqKey="Nugegoda D" first="Dayanthi" last="Nugegoda">Dayanthi Nugegoda</name><affiliation><nlm:affiliation>Ecotoxicology Research Group, School of Applied Sciences, RMIT University , Bowen Street, Melbourne, VIC 3001, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Kaslin, Jan" sort="Kaslin, Jan" uniqKey="Kaslin J" first="Jan" last="Kaslin">Jan Kaslin</name><affiliation><nlm:affiliation>ARMI, Monash University , Wellington Road, Clayton, VIC 3800, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Wlodkowic, Donald" sort="Wlodkowic, Donald" uniqKey="Wlodkowic D" first="Donald" last="Wlodkowic">Donald Wlodkowic</name><affiliation><nlm:affiliation>Centre for Additive Manufacturing, RMIT University , 58 Cardigan Street, Melbourne, VIC 3053, Australia.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Environmental science & technology</title><idno type="eISSN">1520-5851</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Caffeine (toxicity)</term><term>Copper Sulfate (toxicity)</term><term>Dimethyl Sulfoxide (toxicity)</term><term>Embryo, Nonmammalian (drug effects)</term><term>Ethanol (toxicity)</term><term>Lab-On-A-Chip Devices</term><term>Microfluidics (instrumentation)</term><term>Microfluidics (methods)</term><term>Nicotine (toxicity)</term><term>Printing, Three-Dimensional</term><term>Toxicity Tests (instrumentation)</term><term>Toxicity Tests (methods)</term><term>Zebrafish (embryology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Caffeine</term><term>Copper Sulfate</term><term>Dimethyl Sulfoxide</term><term>Ethanol</term><term>Nicotine</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Embryo, Nonmammalian</term></keywords><keywords scheme="MESH" qualifier="embryology" xml:lang="en"><term>Zebrafish</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Microfluidics</term><term>Toxicity Tests</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Microfluidics</term><term>Toxicity Tests</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Lab-On-A-Chip Devices</term><term>Printing, Three-Dimensional</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The fish embryo toxicity (FET) biotest has gained popularity as one of the alternative approaches to acute fish toxicity tests in chemical hazard and risk assessment. Despite the importance and common acceptance of FET, it is still performed in multiwell plates and requires laborious and time-consuming manual manipulation of specimens and solutions. This work describes the design and validation of a microfluidic Lab-on-a-Chip technology for automation of the zebrafish embryo toxicity test common in aquatic ecotoxicology. The innovative device supports rapid loading and immobilization of large numbers of zebrafish embryos suspended in a continuous microfluidic perfusion as a means of toxicant delivery. Furthermore, we also present development of a customized mechatronic automation interface that includes a high-resolution USB microscope, LED cold light illumination, and miniaturized 3D printed pumping manifolds that were integrated to enable time-resolved in situ analysis of developing fish embryos. To investigate the applicability of the microfluidic FET (μFET) in toxicity testing, copper sulfate, phenol, ethanol, caffeine, nicotine, and dimethyl sulfoxide were tested as model chemical stressors. Results obtained on a chip-based system were compared with static protocols performed in microtiter plates. This work provides evidence that FET analysis performed under microperfusion opens a brand new alternative for inexpensive automation in aquatic ecotoxicology.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26506399</PMID><DateCreated><Year>2015</Year><Month>12</Month><Day>15</Day></DateCreated><DateCompleted><Year>2016</Year><Month>07</Month><Day>19</Day></DateCompleted><DateRevised><Year>2015</Year><Month>12</Month><Day>15</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1520-5851</ISSN><JournalIssue CitedMedium="Internet"><Volume>49</Volume><Issue>24</Issue><PubDate><Year>2015</Year><Month>Dec</Month><Day>15</Day></PubDate></JournalIssue><Title>Environmental science & technology</Title><ISOAbbreviation>Environ. Sci. Technol.</ISOAbbreviation></Journal><ArticleTitle>Automated Lab-on-a-Chip Technology for Fish Embryo Toxicity Tests Performed under Continuous Microperfusion (μFET).</ArticleTitle><Pagination><MedlinePgn>14570-8</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1021/acs.est.5b03838</ELocationID><Abstract><AbstractText>The fish embryo toxicity (FET) biotest has gained popularity as one of the alternative approaches to acute fish toxicity tests in chemical hazard and risk assessment. Despite the importance and common acceptance of FET, it is still performed in multiwell plates and requires laborious and time-consuming manual manipulation of specimens and solutions. This work describes the design and validation of a microfluidic Lab-on-a-Chip technology for automation of the zebrafish embryo toxicity test common in aquatic ecotoxicology. The innovative device supports rapid loading and immobilization of large numbers of zebrafish embryos suspended in a continuous microfluidic perfusion as a means of toxicant delivery. Furthermore, we also present development of a customized mechatronic automation interface that includes a high-resolution USB microscope, LED cold light illumination, and miniaturized 3D printed pumping manifolds that were integrated to enable time-resolved in situ analysis of developing fish embryos. To investigate the applicability of the microfluidic FET (μFET) in toxicity testing, copper sulfate, phenol, ethanol, caffeine, nicotine, and dimethyl sulfoxide were tested as model chemical stressors. Results obtained on a chip-based system were compared with static protocols performed in microtiter plates. This work provides evidence that FET analysis performed under microperfusion opens a brand new alternative for inexpensive automation in aquatic ecotoxicology.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhu</LastName><ForeName>Feng</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Wigh</LastName><ForeName>Adriana</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Université de Lyon, UMR LEHNA 5023, USC INRA, ENTPE, rue Maurice Audin, Vaulx-en-Velin F-69518, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Friedrich</LastName><ForeName>Timo</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>ARMI, Monash University , Wellington Road, Clayton, VIC 3800, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Devaux</LastName><ForeName>Alain</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Université de Lyon, UMR LEHNA 5023, USC INRA, ENTPE, rue Maurice Audin, Vaulx-en-Velin F-69518, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bony</LastName><ForeName>Sylvie</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Université de Lyon, UMR LEHNA 5023, USC INRA, ENTPE, rue Maurice Audin, Vaulx-en-Velin F-69518, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nugegoda</LastName><ForeName>Dayanthi</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Ecotoxicology Research Group, School of Applied Sciences, RMIT University , Bowen Street, Melbourne, VIC 3001, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kaslin</LastName><ForeName>Jan</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>ARMI, Monash University , Wellington Road, Clayton, VIC 3800, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wlodkowic</LastName><ForeName>Donald</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Centre for Additive Manufacturing, RMIT University , 58 Cardigan Street, Melbourne, VIC 3053, Australia.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>11</Month><Day>10</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Environ Sci Technol</MedlineTA><NlmUniqueID>0213155</NlmUniqueID><ISSNLinking>0013-936X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>3G6A5W338E</RegistryNumber><NameOfSubstance UI="D002110">Caffeine</NameOfSubstance></Chemical><Chemical><RegistryNumber>3K9958V90M</RegistryNumber><NameOfSubstance UI="D000431">Ethanol</NameOfSubstance></Chemical><Chemical><RegistryNumber>6M3C89ZY6R</RegistryNumber><NameOfSubstance UI="D009538">Nicotine</NameOfSubstance></Chemical><Chemical><RegistryNumber>LRX7AJ16DT</RegistryNumber><NameOfSubstance UI="D019327">Copper Sulfate</NameOfSubstance></Chemical><Chemical><RegistryNumber>YOW8V9698H</RegistryNumber><NameOfSubstance UI="D004121">Dimethyl Sulfoxide</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002110" MajorTopicYN="N">Caffeine</DescriptorName><QualifierName UI="Q000633" MajorTopicYN="N">toxicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019327" MajorTopicYN="N">Copper Sulfate</DescriptorName><QualifierName UI="Q000633" MajorTopicYN="N">toxicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004121" MajorTopicYN="N">Dimethyl Sulfoxide</DescriptorName><QualifierName UI="Q000633" MajorTopicYN="N">toxicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004625" MajorTopicYN="N">Embryo, Nonmammalian</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000431" MajorTopicYN="N">Ethanol</DescriptorName><QualifierName UI="Q000633" MajorTopicYN="N">toxicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D056656" MajorTopicYN="Y">Lab-On-A-Chip Devices</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D044085" MajorTopicYN="N">Microfluidics</DescriptorName><QualifierName UI="Q000295" MajorTopicYN="N">instrumentation</QualifierName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009538" MajorTopicYN="N">Nicotine</DescriptorName><QualifierName UI="Q000633" 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