Perlecan expression influences the keratin 15-positive cell population fate in the epidermis of aging skin.
Identifieur interne : 001F91 ( PubMed/Corpus ); précédent : 001F90; suivant : 001F92Perlecan expression influences the keratin 15-positive cell population fate in the epidermis of aging skin.
Auteurs : Morgan Dos Santos ; Anna Michopoulou ; Valérie André-Frei ; Sophie Boulesteix ; Christine Guicher ; Guila Dayan ; John Whitelock ; Odile Damour ; Patricia RousselleSource :
- Aging [ 1945-4589 ] ; 2016.
English descriptors
- KwdEn :
- Adult, Aged, Cell Differentiation (physiology), Epidermis (cytology), Epidermis (metabolism), Extracellular Matrix (metabolism), Female, Heparan Sulfate Proteoglycans (metabolism), Humans, Keratin-15 (metabolism), Keratinocytes (cytology), Keratinocytes (metabolism), Male, Middle Aged, Skin Aging (physiology), Young Adult.
- MESH :
- chemical , metabolism : Heparan Sulfate Proteoglycans, Keratin-15.
- cytology : Epidermis, Keratinocytes.
- metabolism : Epidermis, Extracellular Matrix, Keratinocytes.
- physiology : Cell Differentiation, Skin Aging.
- Adult, Aged, Female, Humans, Male, Middle Aged, Young Adult.
Abstract
The epidermis is continuously renewed by stem cell proliferation and differentiation. Basal keratinocytes append the dermal-epidermal junction, a cell surface-associated, extracellular matrix that provides structural support and influences their behaviour. It consists of laminins, type IV collagen, nidogens, and perlecan, which are necessary for tissue organization and structural integrity. Perlecan is a heparan sulfate proteoglycan known to be involved in keratinocyte survival and differentiation. Aging affects the dermal epidermal junction resulting in decreased contact with keratinocytes, thus impacting epidermal renewal and homeostasis. We found that perlecan expression decreased during chronological skin aging. Our in vitro studies revealed reduced perlecan transcript levels in aged keratinocytes. The production of in vitro skin models revealed that aged keratinocytes formed a thin and poorly organized epidermis. Supplementing these models with purified perlecan reversed the phenomenon allowing restoration of a well-differentiated multi-layered epithelium. Perlecan down-regulation in cultured keratinocytes caused depletion of the cell population that expressed keratin 15. This phenomenon depended on the perlecan heparan sulphate moieties, which suggested the involvement of a growth factor. Finally, we found defects in keratin 15 expression in the epidermis of aging skin. This study highlighted a new role for perlecan in maintaining the self-renewal capacity of basal keratinocytes.
DOI: 10.18632/aging.100928
PubMed: 26996820
Links to Exploration step
pubmed:26996820Le document en format XML
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André-Frei</name><affiliation><nlm:affiliation>BASF BCS France SAS, 69366, Lyon, France.</nlm:affiliation></affiliation></author><author><name sortKey="Boulesteix, Sophie" sort="Boulesteix, Sophie" uniqKey="Boulesteix S" first="Sophie" last="Boulesteix">Sophie Boulesteix</name><affiliation><nlm:affiliation>Laboratoire de Biologie Tissulaire et Ingénierie Thérapeutique, UMR 5305, CNRS, University Lyon 1, Lyon, France.</nlm:affiliation></affiliation></author><author><name sortKey="Guicher, Christine" sort="Guicher, Christine" uniqKey="Guicher C" first="Christine" last="Guicher">Christine Guicher</name><affiliation><nlm:affiliation>Laboratoire de Biologie Tissulaire et Ingénierie Thérapeutique, UMR 5305, CNRS, University Lyon 1, Lyon, France.</nlm:affiliation></affiliation></author><author><name sortKey="Dayan, Guila" sort="Dayan, Guila" uniqKey="Dayan G" first="Guila" last="Dayan">Guila Dayan</name><affiliation><nlm:affiliation>Laboratoire de Biologie Tissulaire et Ingénierie Thérapeutique, UMR 5305, CNRS, University Lyon 1, Lyon, France.</nlm:affiliation></affiliation></author><author><name sortKey="Whitelock, John" sort="Whitelock, John" uniqKey="Whitelock J" first="John" last="Whitelock">John Whitelock</name><affiliation><nlm:affiliation>Graduate School of Biomedical Engineering, University of New South Wales, Sydney, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Damour, Odile" sort="Damour, Odile" uniqKey="Damour O" first="Odile" last="Damour">Odile Damour</name><affiliation><nlm:affiliation>Laboratoire de Biologie Tissulaire et Ingénierie Thérapeutique, UMR 5305, CNRS, University Lyon 1, Lyon, France.</nlm:affiliation></affiliation></author><author><name sortKey="Rousselle, Patricia" sort="Rousselle, Patricia" uniqKey="Rousselle P" first="Patricia" last="Rousselle">Patricia Rousselle</name><affiliation><nlm:affiliation>Laboratoire de Biologie Tissulaire et Ingénierie Thérapeutique, UMR 5305, CNRS, University Lyon 1, Lyon, 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Anna" uniqKey="Michopoulou A" first="Anna" last="Michopoulou">Anna Michopoulou</name><affiliation><nlm:affiliation>Laboratoire de Biologie Tissulaire et Ingénierie Thérapeutique, UMR 5305, CNRS, University Lyon 1, Lyon, France.</nlm:affiliation></affiliation></author><author><name sortKey="Andre Frei, Valerie" sort="Andre Frei, Valerie" uniqKey="Andre Frei V" first="Valérie" last="André-Frei">Valérie André-Frei</name><affiliation><nlm:affiliation>BASF BCS France SAS, 69366, Lyon, France.</nlm:affiliation></affiliation></author><author><name sortKey="Boulesteix, Sophie" sort="Boulesteix, Sophie" uniqKey="Boulesteix S" first="Sophie" last="Boulesteix">Sophie Boulesteix</name><affiliation><nlm:affiliation>Laboratoire de Biologie Tissulaire et Ingénierie Thérapeutique, UMR 5305, CNRS, University Lyon 1, Lyon, France.</nlm:affiliation></affiliation></author><author><name sortKey="Guicher, Christine" sort="Guicher, Christine" uniqKey="Guicher C" first="Christine" last="Guicher">Christine Guicher</name><affiliation><nlm:affiliation>Laboratoire de Biologie Tissulaire et Ingénierie Thérapeutique, UMR 5305, CNRS, University Lyon 1, Lyon, France.</nlm:affiliation></affiliation></author><author><name sortKey="Dayan, Guila" sort="Dayan, Guila" uniqKey="Dayan G" first="Guila" last="Dayan">Guila Dayan</name><affiliation><nlm:affiliation>Laboratoire de Biologie Tissulaire et Ingénierie Thérapeutique, UMR 5305, CNRS, University Lyon 1, Lyon, France.</nlm:affiliation></affiliation></author><author><name sortKey="Whitelock, John" sort="Whitelock, John" uniqKey="Whitelock J" first="John" last="Whitelock">John Whitelock</name><affiliation><nlm:affiliation>Graduate School of Biomedical Engineering, University of New South Wales, Sydney, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Damour, Odile" sort="Damour, Odile" uniqKey="Damour O" first="Odile" last="Damour">Odile Damour</name><affiliation><nlm:affiliation>Laboratoire de Biologie Tissulaire et Ingénierie Thérapeutique, UMR 5305, CNRS, University Lyon 1, Lyon, France.</nlm:affiliation></affiliation></author><author><name sortKey="Rousselle, Patricia" sort="Rousselle, Patricia" uniqKey="Rousselle P" first="Patricia" last="Rousselle">Patricia Rousselle</name><affiliation><nlm:affiliation>Laboratoire de Biologie Tissulaire et Ingénierie Thérapeutique, UMR 5305, CNRS, University Lyon 1, Lyon, France.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Aging</title><idno type="eISSN">1945-4589</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term><term>Aged</term><term>Cell Differentiation (physiology)</term><term>Epidermis (cytology)</term><term>Epidermis (metabolism)</term><term>Extracellular Matrix (metabolism)</term><term>Female</term><term>Heparan Sulfate Proteoglycans 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Adult</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The epidermis is continuously renewed by stem cell proliferation and differentiation. Basal keratinocytes append the dermal-epidermal junction, a cell surface-associated, extracellular matrix that provides structural support and influences their behaviour. It consists of laminins, type IV collagen, nidogens, and perlecan, which are necessary for tissue organization and structural integrity. Perlecan is a heparan sulfate proteoglycan known to be involved in keratinocyte survival and differentiation. Aging affects the dermal epidermal junction resulting in decreased contact with keratinocytes, thus impacting epidermal renewal and homeostasis. We found that perlecan expression decreased during chronological skin aging. Our in vitro studies revealed reduced perlecan transcript levels in aged keratinocytes. The production of in vitro skin models revealed that aged keratinocytes formed a thin and poorly organized epidermis. Supplementing these models with purified perlecan reversed the phenomenon allowing restoration of a well-differentiated multi-layered epithelium. Perlecan down-regulation in cultured keratinocytes caused depletion of the cell population that expressed keratin 15. This phenomenon depended on the perlecan heparan sulphate moieties, which suggested the involvement of a growth factor. Finally, we found defects in keratin 15 expression in the epidermis of aging skin. This study highlighted a new role for perlecan in maintaining the self-renewal capacity of basal keratinocytes.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26996820</PMID><DateCreated><Year>2016</Year><Month>05</Month><Day>17</Day></DateCreated><DateCompleted><Year>2017</Year><Month>11</Month><Day>03</Day></DateCompleted><DateRevised><Year>2017</Year><Month>11</Month><Day>03</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1945-4589</ISSN><JournalIssue CitedMedium="Internet"><Volume>8</Volume><Issue>4</Issue><PubDate><Year>2016</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Aging</Title><ISOAbbreviation>Aging (Albany NY)</ISOAbbreviation></Journal><ArticleTitle>Perlecan expression influences the keratin 15-positive cell population fate in the epidermis of aging skin.</ArticleTitle><Pagination><MedlinePgn>751-68</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.18632/aging.100928</ELocationID><Abstract><AbstractText>The epidermis is continuously renewed by stem cell proliferation and differentiation. Basal keratinocytes append the dermal-epidermal junction, a cell surface-associated, extracellular matrix that provides structural support and influences their behaviour. It consists of laminins, type IV collagen, nidogens, and perlecan, which are necessary for tissue organization and structural integrity. Perlecan is a heparan sulfate proteoglycan known to be involved in keratinocyte survival and differentiation. Aging affects the dermal epidermal junction resulting in decreased contact with keratinocytes, thus impacting epidermal renewal and homeostasis. We found that perlecan expression decreased during chronological skin aging. Our in vitro studies revealed reduced perlecan transcript levels in aged keratinocytes. The production of in vitro skin models revealed that aged keratinocytes formed a thin and poorly organized epidermis. Supplementing these models with purified perlecan reversed the phenomenon allowing restoration of a well-differentiated multi-layered epithelium. Perlecan down-regulation in cultured keratinocytes caused depletion of the cell population that expressed keratin 15. This phenomenon depended on the perlecan heparan sulphate moieties, which suggested the involvement of a growth factor. Finally, we found defects in keratin 15 expression in the epidermis of aging skin. This study highlighted a new role for perlecan in maintaining the self-renewal capacity of basal keratinocytes.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Dos Santos</LastName><ForeName>Morgan</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Laboratoire de Biologie Tissulaire et Ingénierie Thérapeutique, UMR 5305, CNRS, University Lyon 1, Lyon, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>BASF BCS France SAS, 69366, Lyon, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Michopoulou</LastName><ForeName>Anna</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Laboratoire de Biologie Tissulaire et Ingénierie Thérapeutique, UMR 5305, CNRS, University Lyon 1, Lyon, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>André-Frei</LastName><ForeName>Valérie</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>BASF BCS France SAS, 69366, Lyon, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Boulesteix</LastName><ForeName>Sophie</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Laboratoire de Biologie Tissulaire et Ingénierie Thérapeutique, UMR 5305, CNRS, University Lyon 1, Lyon, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guicher</LastName><ForeName>Christine</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Laboratoire de Biologie Tissulaire et Ingénierie Thérapeutique, UMR 5305, CNRS, University Lyon 1, Lyon, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dayan</LastName><ForeName>Guila</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Laboratoire de Biologie Tissulaire et Ingénierie Thérapeutique, UMR 5305, CNRS, University Lyon 1, Lyon, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Whitelock</LastName><ForeName>John</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Graduate School of Biomedical Engineering, University of New South Wales, Sydney, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Damour</LastName><ForeName>Odile</ForeName><Initials>O</Initials><AffiliationInfo><Affiliation>Laboratoire de Biologie Tissulaire et Ingénierie Thérapeutique, UMR 5305, CNRS, University Lyon 1, Lyon, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Cell and Tissue Bank, Hôpital Edouard Herriot, Lyon, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rousselle</LastName><ForeName>Patricia</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Laboratoire de Biologie Tissulaire et Ingénierie Thérapeutique, UMR 5305, CNRS, University Lyon 1, Lyon, France.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Aging (Albany NY)</MedlineTA><NlmUniqueID>101508617</NlmUniqueID><ISSNLinking>1945-4589</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019812">Heparan Sulfate Proteoglycans</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D053569">Keratin-15</NameOfSubstance></Chemical><Chemical><RegistryNumber>143972-95-6</RegistryNumber><NameOfSubstance UI="C071927">perlecan</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="Cites"><RefSource>Biomed Res Int. 2013;2013:179784</RefSource><PMID 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Version="1">10702276</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Invest Dermatol. 1996 Mar;106(3):531-7</RefSource><PMID Version="1">8648189</PMID></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000368" MajorTopicYN="N">Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002454" MajorTopicYN="N">Cell Differentiation</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004817" MajorTopicYN="N">Epidermis</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005109" MajorTopicYN="N">Extracellular Matrix</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019812" MajorTopicYN="N">Heparan Sulfate Proteoglycans</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053569" MajorTopicYN="N">Keratin-15</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015603" MajorTopicYN="N">Keratinocytes</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008875" MajorTopicYN="N">Middle Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015595" MajorTopicYN="N">Skin Aging</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D055815" MajorTopicYN="N">Young Adult</DescriptorName></MeshHeading></MeshHeadingList><OtherID Source="NLM">PMC4925826</OtherID><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">basement membrane</Keyword><Keyword MajorTopicYN="N">keratin 15</Keyword><Keyword MajorTopicYN="N">keratinocyte</Keyword><Keyword MajorTopicYN="N">perlecan</Keyword><Keyword MajorTopicYN="N">skin aging</Keyword><Keyword MajorTopicYN="N">stem cell</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>01</Month><Day>13</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>02</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>3</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>3</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>11</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26996820</ArticleId><ArticleId IdType="pii">100928</ArticleId><ArticleId IdType="doi">10.18632/aging.100928</ArticleId><ArticleId IdType="pmc">PMC4925826</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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