Distinct immune responses of juvenile and adult oysters (Crassostrea gigas) to viral and bacterial infections.
Identifieur interne : 001C27 ( PubMed/Corpus ); précédent : 001C26; suivant : 001C28Distinct immune responses of juvenile and adult oysters (Crassostrea gigas) to viral and bacterial infections.
Auteurs : Timothy J. Green ; Agnes Vergnes ; Caroline Montagnani ; Julien De LorgerilSource :
- Veterinary research [ 1297-9716 ] ; 2016.
English descriptors
- KwdEn :
- Age Factors, Animals, Crassostrea (genetics), Crassostrea (immunology), Crassostrea (microbiology), Crassostrea (virology), Herpesviridae (immunology), Herpesviridae Infections (immunology), Herpesviridae Infections (veterinary), Herpesviridae Infections (virology), Polymerase Chain Reaction (methods), Vibrio (immunology), Vibrio Infections (immunology), Vibrio Infections (microbiology), Vibrio Infections (veterinary).
- MESH :
- genetics : Crassostrea.
- immunology : Crassostrea, Herpesviridae, Herpesviridae Infections, Vibrio, Vibrio Infections.
- methods : Polymerase Chain Reaction.
- microbiology : Crassostrea, Vibrio Infections.
- veterinary : Herpesviridae Infections, Vibrio Infections.
- virology : Crassostrea, Herpesviridae Infections.
- Age Factors, Animals.
Abstract
Since 2008, massive mortality events of Pacific oysters (Crassostrea gigas) have been reported worldwide and these disease events are often associated with Ostreid herpesvirus type 1 (OsHV-1). Epidemiological field studies have also reported oyster age and other pathogens of the Vibrio genus are contributing factors to this syndrome. We undertook a controlled laboratory experiment to simultaneously investigate survival and immunological response of juvenile and adult C. gigas at different time-points post-infection with OsHV-1, Vibrio tasmaniensis LGP32 and V. aestuarianus. Our data corroborates epidemiological studies that juveniles are more susceptible to OsHV-1, whereas adults are more susceptible to Vibrio. We measured the expression of 102 immune-genes by high-throughput RT-qPCR, which revealed oysters have different transcriptional responses to OsHV-1 and Vibrio. The transcriptional response in the early stages of OsHV-1 infection involved genes related to apoptosis and the interferon-pathway. Transcriptional response to Vibrio infection involved antimicrobial peptides, heat shock proteins and galectins. Interestingly, oysters in the later stages of OsHV-1 infection had a transcriptional response that resembled an antibacterial response, which is suggestive of the oyster's microbiome causing secondary infections (dysbiosis-driven pathology). This study provides molecular evidence that oysters can mount distinct immune response to viral and bacterial pathogens and these responses differ depending on the age of the host.
DOI: 10.1186/s13567-016-0356-7
PubMed: 27439510
Links to Exploration step
pubmed:27439510Le document en format XML
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Montpellier, 34095, Montpellier, France.</nlm:affiliation></affiliation></author><author><name sortKey="Montagnani, Caroline" sort="Montagnani, Caroline" uniqKey="Montagnani C" first="Caroline" last="Montagnani">Caroline Montagnani</name><affiliation><nlm:affiliation>IFREMER, IHPE, UMR 5244, Univ. Perpignan Via Domitia, CNRS, Univ. Montpellier, 34095, Montpellier, France. cmontagn@ifremer.fr.</nlm:affiliation></affiliation></author><author><name sortKey="De Lorgeril, Julien" sort="De Lorgeril, Julien" uniqKey="De Lorgeril J" first="Julien" last="De Lorgeril">Julien De Lorgeril</name><affiliation><nlm:affiliation>IFREMER, IHPE, UMR 5244, Univ. Perpignan Via Domitia, CNRS, Univ. 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Montpellier, 34095, Montpellier, France.</nlm:affiliation></affiliation></author><author><name sortKey="Vergnes, Agnes" sort="Vergnes, Agnes" uniqKey="Vergnes A" first="Agnes" last="Vergnes">Agnes Vergnes</name><affiliation><nlm:affiliation>IFREMER, IHPE, UMR 5244, Univ. Perpignan Via Domitia, CNRS, Univ. Montpellier, 34095, Montpellier, France.</nlm:affiliation></affiliation></author><author><name sortKey="Montagnani, Caroline" sort="Montagnani, Caroline" uniqKey="Montagnani C" first="Caroline" last="Montagnani">Caroline Montagnani</name><affiliation><nlm:affiliation>IFREMER, IHPE, UMR 5244, Univ. Perpignan Via Domitia, CNRS, Univ. Montpellier, 34095, Montpellier, France. cmontagn@ifremer.fr.</nlm:affiliation></affiliation></author><author><name sortKey="De Lorgeril, Julien" sort="De Lorgeril, Julien" uniqKey="De Lorgeril J" first="Julien" last="De Lorgeril">Julien De Lorgeril</name><affiliation><nlm:affiliation>IFREMER, IHPE, UMR 5244, Univ. Perpignan Via Domitia, CNRS, Univ. Montpellier, 34095, Montpellier, France.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Veterinary research</title><idno type="eISSN">1297-9716</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Age Factors</term><term>Animals</term><term>Crassostrea (genetics)</term><term>Crassostrea (immunology)</term><term>Crassostrea (microbiology)</term><term>Crassostrea (virology)</term><term>Herpesviridae (immunology)</term><term>Herpesviridae Infections (immunology)</term><term>Herpesviridae Infections (veterinary)</term><term>Herpesviridae Infections (virology)</term><term>Polymerase Chain Reaction (methods)</term><term>Vibrio (immunology)</term><term>Vibrio Infections (immunology)</term><term>Vibrio Infections (microbiology)</term><term>Vibrio Infections (veterinary)</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Crassostrea</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Crassostrea</term><term>Herpesviridae</term><term>Herpesviridae Infections</term><term>Vibrio</term><term>Vibrio Infections</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Crassostrea</term><term>Vibrio Infections</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Herpesviridae Infections</term><term>Vibrio Infections</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Crassostrea</term><term>Herpesviridae Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Age Factors</term><term>Animals</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Since 2008, massive mortality events of Pacific oysters (Crassostrea gigas) have been reported worldwide and these disease events are often associated with Ostreid herpesvirus type 1 (OsHV-1). Epidemiological field studies have also reported oyster age and other pathogens of the Vibrio genus are contributing factors to this syndrome. We undertook a controlled laboratory experiment to simultaneously investigate survival and immunological response of juvenile and adult C. gigas at different time-points post-infection with OsHV-1, Vibrio tasmaniensis LGP32 and V. aestuarianus. Our data corroborates epidemiological studies that juveniles are more susceptible to OsHV-1, whereas adults are more susceptible to Vibrio. We measured the expression of 102 immune-genes by high-throughput RT-qPCR, which revealed oysters have different transcriptional responses to OsHV-1 and Vibrio. The transcriptional response in the early stages of OsHV-1 infection involved genes related to apoptosis and the interferon-pathway. Transcriptional response to Vibrio infection involved antimicrobial peptides, heat shock proteins and galectins. Interestingly, oysters in the later stages of OsHV-1 infection had a transcriptional response that resembled an antibacterial response, which is suggestive of the oyster's microbiome causing secondary infections (dysbiosis-driven pathology). This study provides molecular evidence that oysters can mount distinct immune response to viral and bacterial pathogens and these responses differ depending on the age of the host.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27439510</PMID><DateCreated><Year>2016</Year><Month>07</Month><Day>21</Day></DateCreated><DateCompleted><Year>2017</Year><Month>09</Month><Day>26</Day></DateCompleted><DateRevised><Year>2017</Year><Month>09</Month><Day>26</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1297-9716</ISSN><JournalIssue CitedMedium="Internet"><Volume>47</Volume><Issue>1</Issue><PubDate><Year>2016</Year><Month>Jul</Month><Day>21</Day></PubDate></JournalIssue><Title>Veterinary research</Title><ISOAbbreviation>Vet. Res.</ISOAbbreviation></Journal><ArticleTitle>Distinct immune responses of juvenile and adult oysters (Crassostrea gigas) to viral and bacterial infections.</ArticleTitle><Pagination><MedlinePgn>72</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/s13567-016-0356-7</ELocationID><Abstract><AbstractText>Since 2008, massive mortality events of Pacific oysters (Crassostrea gigas) have been reported worldwide and these disease events are often associated with Ostreid herpesvirus type 1 (OsHV-1). Epidemiological field studies have also reported oyster age and other pathogens of the Vibrio genus are contributing factors to this syndrome. We undertook a controlled laboratory experiment to simultaneously investigate survival and immunological response of juvenile and adult C. gigas at different time-points post-infection with OsHV-1, Vibrio tasmaniensis LGP32 and V. aestuarianus. Our data corroborates epidemiological studies that juveniles are more susceptible to OsHV-1, whereas adults are more susceptible to Vibrio. We measured the expression of 102 immune-genes by high-throughput RT-qPCR, which revealed oysters have different transcriptional responses to OsHV-1 and Vibrio. The transcriptional response in the early stages of OsHV-1 infection involved genes related to apoptosis and the interferon-pathway. Transcriptional response to Vibrio infection involved antimicrobial peptides, heat shock proteins and galectins. Interestingly, oysters in the later stages of OsHV-1 infection had a transcriptional response that resembled an antibacterial response, which is suggestive of the oyster's microbiome causing secondary infections (dysbiosis-driven pathology). This study provides molecular evidence that oysters can mount distinct immune response to viral and bacterial pathogens and these responses differ depending on the age of the host.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Green</LastName><ForeName>Timothy J</ForeName><Initials>TJ</Initials><AffiliationInfo><Affiliation>IFREMER, IHPE, UMR 5244, Univ. Perpignan Via Domitia, CNRS, Univ. Montpellier, 34095, Montpellier, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Biological Sciences, Macquarie University, Sydney, NSW, 2109, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vergnes</LastName><ForeName>Agnes</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>IFREMER, IHPE, UMR 5244, Univ. Perpignan Via Domitia, CNRS, Univ. Montpellier, 34095, Montpellier, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Montagnani</LastName><ForeName>Caroline</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>IFREMER, IHPE, UMR 5244, Univ. Perpignan Via Domitia, CNRS, Univ. Montpellier, 34095, Montpellier, France. cmontagn@ifremer.fr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>de Lorgeril</LastName><ForeName>Julien</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>IFREMER, IHPE, UMR 5244, Univ. Perpignan Via Domitia, CNRS, Univ. Montpellier, 34095, Montpellier, France.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>07</Month><Day>21</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Vet Res</MedlineTA><NlmUniqueID>9309551</NlmUniqueID><ISSNLinking>0928-4249</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="Cites"><RefSource>Environ Microbiol. 2015 Nov;17(11):4200-12</RefSource><PMID Version="1">25384719</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Environ Microbiol. 2010 Apr;12(4):951-63</RefSource><PMID Version="1">20074236</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Appl Microbiol. 2006 Apr;100(4):664-72</RefSource><PMID 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