Apicoplast-Localized Lysophosphatidic Acid Precursor Assembly Is Required for Bulk Phospholipid Synthesis in Toxoplasma gondii and Relies on an Algal/Plant-Like Glycerol 3-Phosphate Acyltransferase.
Identifieur interne : 001B06 ( PubMed/Corpus ); précédent : 001B05; suivant : 001B07Apicoplast-Localized Lysophosphatidic Acid Precursor Assembly Is Required for Bulk Phospholipid Synthesis in Toxoplasma gondii and Relies on an Algal/Plant-Like Glycerol 3-Phosphate Acyltransferase.
Auteurs : Souad Amiar ; James I. Macrae ; Damien L. Callahan ; David Dubois ; Giel G. Van Dooren ; Melanie J. Shears ; Marie-France Cesbron-Delauw ; Eric Maréchal ; Malcolm J. Mcconville ; Geoffrey I. Mcfadden ; Yoshiki Yamaryo-Botté ; Cyrille Y. BottéSource :
- PLoS pathogens [ 1553-7374 ] ; 2016.
English descriptors
- KwdEn :
- Amino Acid Sequence, Apicoplasts (enzymology), Chromatography, Liquid, Fluorescent Antibody Technique, Gene Knockdown Techniques, Glycerol-3-Phosphate O-Acyltransferase (metabolism), Lysophospholipids (biosynthesis), Mass Spectrometry, Microscopy, Electron, Transmission, Models, Molecular, Phospholipids (biosynthesis), Phylogeny, Polymerase Chain Reaction, Protozoan Proteins (biosynthesis), Protozoan Proteins (chemistry), Toxoplasma (metabolism).
- MESH :
- chemical , biosynthesis : Lysophospholipids, Phospholipids, Protozoan Proteins.
- chemical , chemistry : Protozoan Proteins.
- chemical , metabolism : Glycerol-3-Phosphate O-Acyltransferase.
- enzymology : Apicoplasts.
- metabolism : Toxoplasma.
- Amino Acid Sequence, Chromatography, Liquid, Fluorescent Antibody Technique, Gene Knockdown Techniques, Mass Spectrometry, Microscopy, Electron, Transmission, Models, Molecular, Phylogeny, Polymerase Chain Reaction.
Abstract
Most apicomplexan parasites possess a non-photosynthetic plastid (the apicoplast), which harbors enzymes for a number of metabolic pathways, including a prokaryotic type II fatty acid synthesis (FASII) pathway. In Toxoplasma gondii, the causative agent of toxoplasmosis, the FASII pathway is essential for parasite growth and infectivity. However, little is known about the fate of fatty acids synthesized by FASII. In this study, we have investigated the function of a plant-like glycerol 3-phosphate acyltransferase (TgATS1) that localizes to the T. gondii apicoplast. Knock-down of TgATS1 resulted in significantly reduced incorporation of FASII-synthesized fatty acids into phosphatidic acid and downstream phospholipids and a severe defect in intracellular parasite replication and survival. Lipidomic analysis demonstrated that lipid precursors are made in, and exported from, the apicoplast for de novo biosynthesis of bulk phospholipids. This study reveals that the apicoplast-located FASII and ATS1, which are primarily used to generate plastid galactolipids in plants and algae, instead generate bulk phospholipids for membrane biogenesis in T. gondii.
DOI: 10.1371/journal.ppat.1005765
PubMed: 27490259
Links to Exploration step
pubmed:27490259Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Apicoplast-Localized Lysophosphatidic Acid Precursor Assembly Is Required for Bulk Phospholipid Synthesis in Toxoplasma gondii and Relies on an Algal/Plant-Like Glycerol 3-Phosphate Acyltransferase.</title><author><name sortKey="Amiar, Souad" sort="Amiar, Souad" uniqKey="Amiar S" first="Souad" last="Amiar">Souad Amiar</name><affiliation><nlm:affiliation>ApicoLipid group, Institute for Advanced Biosciences UMR5309, CNRS, Université Grenoble Alpes, INSERM, Grenoble, France.</nlm:affiliation></affiliation></author><author><name sortKey="Macrae, James I" sort="Macrae, James I" uniqKey="Macrae J" first="James I" last="Macrae">James I. Macrae</name><affiliation><nlm:affiliation>The Francis Crick Institute, The Ridgeway, Mill Hill, London, United Kingdom.</nlm:affiliation></affiliation></author><author><name sortKey="Callahan, Damien L" sort="Callahan, Damien L" uniqKey="Callahan D" first="Damien L" last="Callahan">Damien L. Callahan</name><affiliation><nlm:affiliation>Centre for Chemistry and Biotechnology, School of Life and Environmental Sciences, Deakin University, Burwood, Victoria, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Dubois, David" sort="Dubois, David" uniqKey="Dubois D" first="David" last="Dubois">David Dubois</name><affiliation><nlm:affiliation>ApicoLipid group, Institute for Advanced Biosciences UMR5309, CNRS, Université Grenoble Alpes, INSERM, Grenoble, France.</nlm:affiliation></affiliation></author><author><name sortKey="Van Dooren, Giel G" sort="Van Dooren, Giel G" uniqKey="Van Dooren G" first="Giel G" last="Van Dooren">Giel G. Van Dooren</name><affiliation><nlm:affiliation>Research School of Biology, Australian National University, Canberra, Australian Capital Territory, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Shears, Melanie J" sort="Shears, Melanie J" uniqKey="Shears M" first="Melanie J" last="Shears">Melanie J. 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Mcconville</name><affiliation><nlm:affiliation>Department of Biochemistry and Molecular Biology, Bio21 Molecular Science & Biotechnology Institute, University of Melbourne, Melbourne, Victoria, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Mcfadden, Geoffrey I" sort="Mcfadden, Geoffrey I" uniqKey="Mcfadden G" first="Geoffrey I" last="Mcfadden">Geoffrey I. Mcfadden</name><affiliation><nlm:affiliation>School of BioSciences, University of Melbourne, Melbourne, Victoria, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Yamaryo Botte, Yoshiki" sort="Yamaryo Botte, Yoshiki" uniqKey="Yamaryo Botte Y" first="Yoshiki" last="Yamaryo-Botté">Yoshiki Yamaryo-Botté</name><affiliation><nlm:affiliation>ApicoLipid group, Institute for Advanced Biosciences UMR5309, CNRS, Université Grenoble Alpes, INSERM, Grenoble, France.</nlm:affiliation></affiliation></author><author><name sortKey="Botte, Cyrille Y" sort="Botte, Cyrille Y" uniqKey="Botte C" first="Cyrille Y" last="Botté">Cyrille Y. Botté</name><affiliation><nlm:affiliation>ApicoLipid group, Institute for Advanced Biosciences UMR5309, CNRS, Université Grenoble Alpes, INSERM, Grenoble, France.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:27490259</idno><idno type="pmid">27490259</idno><idno type="doi">10.1371/journal.ppat.1005765</idno><idno type="wicri:Area/PubMed/Corpus">001B06</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001B06</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Apicoplast-Localized Lysophosphatidic Acid Precursor Assembly Is Required for Bulk Phospholipid Synthesis in Toxoplasma gondii and Relies on an Algal/Plant-Like Glycerol 3-Phosphate Acyltransferase.</title><author><name sortKey="Amiar, Souad" sort="Amiar, Souad" uniqKey="Amiar S" first="Souad" last="Amiar">Souad Amiar</name><affiliation><nlm:affiliation>ApicoLipid group, Institute for Advanced Biosciences UMR5309, CNRS, Université Grenoble Alpes, INSERM, Grenoble, France.</nlm:affiliation></affiliation></author><author><name sortKey="Macrae, James I" sort="Macrae, James I" uniqKey="Macrae J" first="James I" last="Macrae">James I. Macrae</name><affiliation><nlm:affiliation>The Francis Crick Institute, The Ridgeway, Mill Hill, London, United Kingdom.</nlm:affiliation></affiliation></author><author><name sortKey="Callahan, Damien L" sort="Callahan, Damien L" uniqKey="Callahan D" first="Damien L" last="Callahan">Damien L. Callahan</name><affiliation><nlm:affiliation>Centre for Chemistry and Biotechnology, School of Life and Environmental Sciences, Deakin University, Burwood, Victoria, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Dubois, David" sort="Dubois, David" uniqKey="Dubois D" first="David" last="Dubois">David Dubois</name><affiliation><nlm:affiliation>ApicoLipid group, Institute for Advanced Biosciences UMR5309, CNRS, Université Grenoble Alpes, INSERM, Grenoble, France.</nlm:affiliation></affiliation></author><author><name sortKey="Van Dooren, Giel G" sort="Van Dooren, Giel G" uniqKey="Van Dooren G" first="Giel G" last="Van Dooren">Giel G. Van Dooren</name><affiliation><nlm:affiliation>Research School of Biology, Australian National University, Canberra, Australian Capital Territory, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Shears, Melanie J" sort="Shears, Melanie J" uniqKey="Shears M" first="Melanie J" last="Shears">Melanie J. Shears</name><affiliation><nlm:affiliation>ApicoLipid group, Institute for Advanced Biosciences UMR5309, CNRS, Université Grenoble Alpes, INSERM, Grenoble, France.</nlm:affiliation></affiliation></author><author><name sortKey="Cesbron Delauw, Marie France" sort="Cesbron Delauw, Marie France" uniqKey="Cesbron Delauw M" first="Marie-France" last="Cesbron-Delauw">Marie-France Cesbron-Delauw</name><affiliation><nlm:affiliation>BNI group, TIMC-IMAG UMR5525 CNRS, Université Grenoble Alpes, Grenoble, France.</nlm:affiliation></affiliation></author><author><name sortKey="Marechal, Eric" sort="Marechal, Eric" uniqKey="Marechal E" first="Eric" last="Maréchal">Eric Maréchal</name><affiliation><nlm:affiliation>Unité de recherche (UMR) 5168, CNRS, CEA, INRA, Université Grenoble Alpes, Grenoble, France.</nlm:affiliation></affiliation></author><author><name sortKey="Mcconville, Malcolm J" sort="Mcconville, Malcolm J" uniqKey="Mcconville M" first="Malcolm J" last="Mcconville">Malcolm J. Mcconville</name><affiliation><nlm:affiliation>Department of Biochemistry and Molecular Biology, Bio21 Molecular Science & Biotechnology Institute, University of Melbourne, Melbourne, Victoria, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Mcfadden, Geoffrey I" sort="Mcfadden, Geoffrey I" uniqKey="Mcfadden G" first="Geoffrey I" last="Mcfadden">Geoffrey I. Mcfadden</name><affiliation><nlm:affiliation>School of BioSciences, University of Melbourne, Melbourne, Victoria, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Yamaryo Botte, Yoshiki" sort="Yamaryo Botte, Yoshiki" uniqKey="Yamaryo Botte Y" first="Yoshiki" last="Yamaryo-Botté">Yoshiki Yamaryo-Botté</name><affiliation><nlm:affiliation>ApicoLipid group, Institute for Advanced Biosciences UMR5309, CNRS, Université Grenoble Alpes, INSERM, Grenoble, France.</nlm:affiliation></affiliation></author><author><name sortKey="Botte, Cyrille Y" sort="Botte, Cyrille Y" uniqKey="Botte C" first="Cyrille Y" last="Botté">Cyrille Y. Botté</name><affiliation><nlm:affiliation>ApicoLipid group, Institute for Advanced Biosciences UMR5309, CNRS, Université Grenoble Alpes, INSERM, Grenoble, France.</nlm:affiliation></affiliation></author></analytic><series><title level="j">PLoS pathogens</title><idno type="eISSN">1553-7374</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Apicoplasts (enzymology)</term><term>Chromatography, Liquid</term><term>Fluorescent Antibody Technique</term><term>Gene Knockdown Techniques</term><term>Glycerol-3-Phosphate O-Acyltransferase (metabolism)</term><term>Lysophospholipids (biosynthesis)</term><term>Mass Spectrometry</term><term>Microscopy, Electron, Transmission</term><term>Models, Molecular</term><term>Phospholipids (biosynthesis)</term><term>Phylogeny</term><term>Polymerase Chain Reaction</term><term>Protozoan Proteins (biosynthesis)</term><term>Protozoan Proteins (chemistry)</term><term>Toxoplasma (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Lysophospholipids</term><term>Phospholipids</term><term>Protozoan Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Protozoan Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Glycerol-3-Phosphate O-Acyltransferase</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Apicoplasts</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Toxoplasma</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Chromatography, Liquid</term><term>Fluorescent Antibody Technique</term><term>Gene Knockdown Techniques</term><term>Mass Spectrometry</term><term>Microscopy, Electron, Transmission</term><term>Models, Molecular</term><term>Phylogeny</term><term>Polymerase Chain Reaction</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Most apicomplexan parasites possess a non-photosynthetic plastid (the apicoplast), which harbors enzymes for a number of metabolic pathways, including a prokaryotic type II fatty acid synthesis (FASII) pathway. In Toxoplasma gondii, the causative agent of toxoplasmosis, the FASII pathway is essential for parasite growth and infectivity. However, little is known about the fate of fatty acids synthesized by FASII. In this study, we have investigated the function of a plant-like glycerol 3-phosphate acyltransferase (TgATS1) that localizes to the T. gondii apicoplast. Knock-down of TgATS1 resulted in significantly reduced incorporation of FASII-synthesized fatty acids into phosphatidic acid and downstream phospholipids and a severe defect in intracellular parasite replication and survival. Lipidomic analysis demonstrated that lipid precursors are made in, and exported from, the apicoplast for de novo biosynthesis of bulk phospholipids. This study reveals that the apicoplast-located FASII and ATS1, which are primarily used to generate plastid galactolipids in plants and algae, instead generate bulk phospholipids for membrane biogenesis in T. gondii.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27490259</PMID><DateCreated><Year>2016</Year><Month>08</Month><Day>05</Day></DateCreated><DateCompleted><Year>2017</Year><Month>06</Month><Day>23</Day></DateCompleted><DateRevised><Year>2017</Year><Month>06</Month><Day>23</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Electronic">1553-7374</ISSN><JournalIssue CitedMedium="Internet"><Volume>12</Volume><Issue>8</Issue><PubDate><Year>2016</Year><Month>Aug</Month></PubDate></JournalIssue><Title>PLoS pathogens</Title><ISOAbbreviation>PLoS Pathog.</ISOAbbreviation></Journal><ArticleTitle>Apicoplast-Localized Lysophosphatidic Acid Precursor Assembly Is Required for Bulk Phospholipid Synthesis in Toxoplasma gondii and Relies on an Algal/Plant-Like Glycerol 3-Phosphate Acyltransferase.</ArticleTitle><Pagination><MedlinePgn>e1005765</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1371/journal.ppat.1005765</ELocationID><Abstract><AbstractText>Most apicomplexan parasites possess a non-photosynthetic plastid (the apicoplast), which harbors enzymes for a number of metabolic pathways, including a prokaryotic type II fatty acid synthesis (FASII) pathway. In Toxoplasma gondii, the causative agent of toxoplasmosis, the FASII pathway is essential for parasite growth and infectivity. However, little is known about the fate of fatty acids synthesized by FASII. In this study, we have investigated the function of a plant-like glycerol 3-phosphate acyltransferase (TgATS1) that localizes to the T. gondii apicoplast. Knock-down of TgATS1 resulted in significantly reduced incorporation of FASII-synthesized fatty acids into phosphatidic acid and downstream phospholipids and a severe defect in intracellular parasite replication and survival. Lipidomic analysis demonstrated that lipid precursors are made in, and exported from, the apicoplast for de novo biosynthesis of bulk phospholipids. This study reveals that the apicoplast-located FASII and ATS1, which are primarily used to generate plastid galactolipids in plants and algae, instead generate bulk phospholipids for membrane biogenesis in T. gondii.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Amiar</LastName><ForeName>Souad</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>ApicoLipid group, Institute for Advanced Biosciences UMR5309, CNRS, Université Grenoble Alpes, INSERM, Grenoble, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>MacRae</LastName><ForeName>James I</ForeName><Initials>JI</Initials><AffiliationInfo><Affiliation>The Francis Crick Institute, The Ridgeway, Mill Hill, London, United Kingdom.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Biochemistry and Molecular Biology, Bio21 Molecular Science & Biotechnology Institute, University of Melbourne, Melbourne, Victoria, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Callahan</LastName><ForeName>Damien L</ForeName><Initials>DL</Initials><AffiliationInfo><Affiliation>Centre for Chemistry and Biotechnology, School of Life and Environmental Sciences, Deakin University, Burwood, Victoria, Australia.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>School of BioSciences, University of Melbourne, Melbourne, Victoria, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dubois</LastName><ForeName>David</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>ApicoLipid group, Institute for Advanced Biosciences UMR5309, CNRS, Université Grenoble Alpes, INSERM, Grenoble, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>van Dooren</LastName><ForeName>Giel G</ForeName><Initials>GG</Initials><AffiliationInfo><Affiliation>Research School of Biology, Australian National University, Canberra, Australian Capital Territory, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shears</LastName><ForeName>Melanie J</ForeName><Initials>MJ</Initials><AffiliationInfo><Affiliation>ApicoLipid group, Institute for Advanced Biosciences UMR5309, CNRS, Université Grenoble Alpes, INSERM, Grenoble, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>School of BioSciences, University of Melbourne, Melbourne, Victoria, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cesbron-Delauw</LastName><ForeName>Marie-France</ForeName><Initials>MF</Initials><AffiliationInfo><Affiliation>BNI group, TIMC-IMAG UMR5525 CNRS, Université Grenoble Alpes, Grenoble, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Maréchal</LastName><ForeName>Eric</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Unité de recherche (UMR) 5168, CNRS, CEA, INRA, Université Grenoble Alpes, Grenoble, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>McConville</LastName><ForeName>Malcolm J</ForeName><Initials>MJ</Initials><AffiliationInfo><Affiliation>Department of Biochemistry and Molecular Biology, Bio21 Molecular Science & Biotechnology Institute, University of Melbourne, Melbourne, Victoria, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>McFadden</LastName><ForeName>Geoffrey I</ForeName><Initials>GI</Initials><AffiliationInfo><Affiliation>School of BioSciences, University of Melbourne, Melbourne, Victoria, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yamaryo-Botté</LastName><ForeName>Yoshiki</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>ApicoLipid group, Institute for Advanced Biosciences UMR5309, CNRS, Université Grenoble Alpes, INSERM, Grenoble, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Botté</LastName><ForeName>Cyrille Y</ForeName><Initials>CY</Initials><AffiliationInfo><Affiliation>ApicoLipid group, Institute for Advanced Biosciences UMR5309, CNRS, Université Grenoble Alpes, INSERM, Grenoble, France.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>08</Month><Day>04</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>PLoS Pathog</MedlineTA><NlmUniqueID>101238921</NlmUniqueID><ISSNLinking>1553-7366</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008246">Lysophospholipids</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010743">Phospholipids</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015800">Protozoan Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.3.1.15</RegistryNumber><NameOfSubstance UI="D005992">Glycerol-3-Phosphate O-Acyltransferase</NameOfSubstance></Chemical><Chemical><RegistryNumber>PG6M3969SG</RegistryNumber><NameOfSubstance UI="C032881">lysophosphatidic acid</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="Cites"><RefSource>Proc Natl Acad Sci U S A. 2006 Aug 29;103(35):13192-7</RefSource><PMID Version="1">16920791</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Proc Natl Acad Sci U S A. 2004 Oct 26;101(43):15458-63</RefSource><PMID Version="1">15492221</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Biol Chem. 2009 Nov 27;284(48):33683-91</RefSource><PMID Version="1">19808683</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Cell Host Microbe. 2010 Jan 21;7(1):62-73</RefSource><PMID 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Version="1">22607142</PMID></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D063546" MajorTopicYN="N">Apicoplasts</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002853" MajorTopicYN="N">Chromatography, Liquid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005455" MajorTopicYN="N">Fluorescent Antibody Technique</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055785" MajorTopicYN="N">Gene Knockdown Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005992" MajorTopicYN="N">Glycerol-3-Phosphate O-Acyltransferase</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008246" MajorTopicYN="N">Lysophospholipids</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013058" MajorTopicYN="N">Mass Spectrometry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D046529" MajorTopicYN="N">Microscopy, Electron, Transmission</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008958" MajorTopicYN="N">Models, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010743" MajorTopicYN="N">Phospholipids</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015800" MajorTopicYN="N">Protozoan Proteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014122" MajorTopicYN="N">Toxoplasma</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList><OtherID Source="NLM">PMC4973916</OtherID></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>07</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>06</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>8</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>8</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>6</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27490259</ArticleId><ArticleId IdType="doi">10.1371/journal.ppat.1005765</ArticleId><ArticleId IdType="pii">PPATHOGENS-D-15-01667</ArticleId><ArticleId IdType="pmc">PMC4973916</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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