Low-dose tamoxifen treatment in juvenile males has long-term adverse effects on the reproductive system: implications for inducible transgenics.
Identifieur interne : 000697 ( PubMed/Corpus ); précédent : 000696; suivant : 000698Low-dose tamoxifen treatment in juvenile males has long-term adverse effects on the reproductive system: implications for inducible transgenics.
Auteurs : Saloni H. Patel ; Laura O'Hara ; Nina Atanassova ; Sarah E. Smith ; Michael K. Curley ; Diane Rebourcet ; Annalucia L. Darbey ; Anne-Louise Gannon ; Richard M. Sharpe ; Lee B. SmithSource :
- Scientific reports [ 2045-2322 ] ; 2017.
Abstract
The tamoxifen-inducible Cre system is a popular transgenic method for controlling the induction of recombination by Cre at a specific time and in a specific cell type. However, tamoxifen is not an inert inducer of recombination, but an established endocrine disruptor with mixed agonist/antagonist activity acting via endogenous estrogen receptors. Such potentially confounding effects should be controlled for, but >40% of publications that have used tamoxifen to generate conditional knockouts have not reported even the minimum appropriate controls. To highlight the importance of this issue, the present study investigated the long-term impacts of different doses of a single systemic tamoxifen injection on the testis and the wider endocrine system. We found that a single dose of tamoxifen less than 10% of the mean dose used for recombination induction, caused adverse effects to the testis and to the reproductive endocrine system that persisted long-term. These data raise significant concerns about the widespread use of tamoxifen induction of recombination, and highlight the importance of including appropriate controls in all pathophysiological studies using this means of induction.
DOI: 10.1038/s41598-017-09016-4
PubMed: 28827578
Links to Exploration step
pubmed:28827578Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Low-dose tamoxifen treatment in juvenile males has long-term adverse effects on the reproductive system: implications for inducible transgenics.</title><author><name sortKey="Patel, Saloni H" sort="Patel, Saloni H" uniqKey="Patel S" first="Saloni H" last="Patel">Saloni H. Patel</name><affiliation><nlm:affiliation>MRC Centre for Reproductive Health, The Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK.</nlm:affiliation></affiliation></author><author><name sortKey="O Hara, Laura" sort="O Hara, Laura" uniqKey="O Hara L" first="Laura" last="O'Hara">Laura O'Hara</name><affiliation><nlm:affiliation>MRC Centre for Reproductive Health, The Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK.</nlm:affiliation></affiliation></author><author><name sortKey="Atanassova, Nina" sort="Atanassova, Nina" uniqKey="Atanassova N" first="Nina" last="Atanassova">Nina Atanassova</name><affiliation><nlm:affiliation>Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Sofia, Bulgaria.</nlm:affiliation></affiliation></author><author><name sortKey="Smith, Sarah E" sort="Smith, Sarah E" uniqKey="Smith S" first="Sarah E" last="Smith">Sarah E. Smith</name><affiliation><nlm:affiliation>MRC Centre for Reproductive Health, The Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK.</nlm:affiliation></affiliation></author><author><name sortKey="Curley, Michael K" sort="Curley, Michael K" uniqKey="Curley M" first="Michael K" last="Curley">Michael K. Curley</name><affiliation><nlm:affiliation>MRC Centre for Reproductive Health, The Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK.</nlm:affiliation></affiliation></author><author><name sortKey="Rebourcet, Diane" sort="Rebourcet, Diane" uniqKey="Rebourcet D" first="Diane" last="Rebourcet">Diane Rebourcet</name><affiliation><nlm:affiliation>MRC Centre for Reproductive Health, The Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK.</nlm:affiliation></affiliation></author><author><name sortKey="Darbey, Annalucia L" sort="Darbey, Annalucia L" uniqKey="Darbey A" first="Annalucia L" last="Darbey">Annalucia L. Darbey</name><affiliation><nlm:affiliation>MRC Centre for Reproductive Health, The Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK.</nlm:affiliation></affiliation></author><author><name sortKey="Gannon, Anne Louise" sort="Gannon, Anne Louise" uniqKey="Gannon A" first="Anne-Louise" last="Gannon">Anne-Louise Gannon</name><affiliation><nlm:affiliation>MRC Centre for Reproductive Health, The Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK.</nlm:affiliation></affiliation></author><author><name sortKey="Sharpe, Richard M" sort="Sharpe, Richard M" uniqKey="Sharpe R" first="Richard M" last="Sharpe">Richard M. Sharpe</name><affiliation><nlm:affiliation>MRC Centre for Reproductive Health, The Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK.</nlm:affiliation></affiliation></author><author><name sortKey="Smith, Lee B" sort="Smith, Lee B" uniqKey="Smith L" first="Lee B" last="Smith">Lee B. Smith</name><affiliation><nlm:affiliation>MRC Centre for Reproductive Health, The Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK. Lee.Smith@ed.ac.uk.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:28827578</idno><idno type="pmid">28827578</idno><idno type="doi">10.1038/s41598-017-09016-4</idno><idno type="wicri:Area/PubMed/Corpus">000697</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000697</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Low-dose tamoxifen treatment in juvenile males has long-term adverse effects on the reproductive system: implications for inducible transgenics.</title><author><name sortKey="Patel, Saloni H" sort="Patel, Saloni H" uniqKey="Patel S" first="Saloni H" last="Patel">Saloni H. Patel</name><affiliation><nlm:affiliation>MRC Centre for Reproductive Health, The Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK.</nlm:affiliation></affiliation></author><author><name sortKey="O Hara, Laura" sort="O Hara, Laura" uniqKey="O Hara L" first="Laura" last="O'Hara">Laura O'Hara</name><affiliation><nlm:affiliation>MRC Centre for Reproductive Health, The Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK.</nlm:affiliation></affiliation></author><author><name sortKey="Atanassova, Nina" sort="Atanassova, Nina" uniqKey="Atanassova N" first="Nina" last="Atanassova">Nina Atanassova</name><affiliation><nlm:affiliation>Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Sofia, Bulgaria.</nlm:affiliation></affiliation></author><author><name sortKey="Smith, Sarah E" sort="Smith, Sarah E" uniqKey="Smith S" first="Sarah E" last="Smith">Sarah E. Smith</name><affiliation><nlm:affiliation>MRC Centre for Reproductive Health, The Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK.</nlm:affiliation></affiliation></author><author><name sortKey="Curley, Michael K" sort="Curley, Michael K" uniqKey="Curley M" first="Michael K" last="Curley">Michael K. Curley</name><affiliation><nlm:affiliation>MRC Centre for Reproductive Health, The Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK.</nlm:affiliation></affiliation></author><author><name sortKey="Rebourcet, Diane" sort="Rebourcet, Diane" uniqKey="Rebourcet D" first="Diane" last="Rebourcet">Diane Rebourcet</name><affiliation><nlm:affiliation>MRC Centre for Reproductive Health, The Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK.</nlm:affiliation></affiliation></author><author><name sortKey="Darbey, Annalucia L" sort="Darbey, Annalucia L" uniqKey="Darbey A" first="Annalucia L" last="Darbey">Annalucia L. Darbey</name><affiliation><nlm:affiliation>MRC Centre for Reproductive Health, The Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK.</nlm:affiliation></affiliation></author><author><name sortKey="Gannon, Anne Louise" sort="Gannon, Anne Louise" uniqKey="Gannon A" first="Anne-Louise" last="Gannon">Anne-Louise Gannon</name><affiliation><nlm:affiliation>MRC Centre for Reproductive Health, The Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK.</nlm:affiliation></affiliation></author><author><name sortKey="Sharpe, Richard M" sort="Sharpe, Richard M" uniqKey="Sharpe R" first="Richard M" last="Sharpe">Richard M. Sharpe</name><affiliation><nlm:affiliation>MRC Centre for Reproductive Health, The Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK.</nlm:affiliation></affiliation></author><author><name sortKey="Smith, Lee B" sort="Smith, Lee B" uniqKey="Smith L" first="Lee B" last="Smith">Lee B. Smith</name><affiliation><nlm:affiliation>MRC Centre for Reproductive Health, The Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK. Lee.Smith@ed.ac.uk.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Scientific reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The tamoxifen-inducible Cre system is a popular transgenic method for controlling the induction of recombination by Cre at a specific time and in a specific cell type. However, tamoxifen is not an inert inducer of recombination, but an established endocrine disruptor with mixed agonist/antagonist activity acting via endogenous estrogen receptors. Such potentially confounding effects should be controlled for, but >40% of publications that have used tamoxifen to generate conditional knockouts have not reported even the minimum appropriate controls. To highlight the importance of this issue, the present study investigated the long-term impacts of different doses of a single systemic tamoxifen injection on the testis and the wider endocrine system. We found that a single dose of tamoxifen less than 10% of the mean dose used for recombination induction, caused adverse effects to the testis and to the reproductive endocrine system that persisted long-term. These data raise significant concerns about the widespread use of tamoxifen induction of recombination, and highlight the importance of including appropriate controls in all pathophysiological studies using this means of induction.</div></front></TEI><pubmed><MedlineCitation Status="In-Data-Review" Owner="NLM"><PMID Version="1">28827578</PMID><DateCreated><Year>2017</Year><Month>08</Month><Day>22</Day></DateCreated><DateRevised><Year>2017</Year><Month>08</Month><Day>25</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2045-2322</ISSN><JournalIssue CitedMedium="Internet"><Volume>7</Volume><Issue>1</Issue><PubDate><Year>2017</Year><Month>Aug</Month><Day>21</Day></PubDate></JournalIssue><Title>Scientific reports</Title><ISOAbbreviation>Sci Rep</ISOAbbreviation></Journal><ArticleTitle>Low-dose tamoxifen treatment in juvenile males has long-term adverse effects on the reproductive system: implications for inducible transgenics.</ArticleTitle><Pagination><MedlinePgn>8991</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1038/s41598-017-09016-4</ELocationID><Abstract><AbstractText>The tamoxifen-inducible Cre system is a popular transgenic method for controlling the induction of recombination by Cre at a specific time and in a specific cell type. However, tamoxifen is not an inert inducer of recombination, but an established endocrine disruptor with mixed agonist/antagonist activity acting via endogenous estrogen receptors. Such potentially confounding effects should be controlled for, but >40% of publications that have used tamoxifen to generate conditional knockouts have not reported even the minimum appropriate controls. To highlight the importance of this issue, the present study investigated the long-term impacts of different doses of a single systemic tamoxifen injection on the testis and the wider endocrine system. We found that a single dose of tamoxifen less than 10% of the mean dose used for recombination induction, caused adverse effects to the testis and to the reproductive endocrine system that persisted long-term. These data raise significant concerns about the widespread use of tamoxifen induction of recombination, and highlight the importance of including appropriate controls in all pathophysiological studies using this means of induction.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Patel</LastName><ForeName>Saloni H</ForeName><Initials>SH</Initials><AffiliationInfo><Affiliation>MRC Centre for Reproductive Health, The Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>O'Hara</LastName><ForeName>Laura</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>MRC Centre for Reproductive Health, The Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Atanassova</LastName><ForeName>Nina</ForeName><Initials>N</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-9032-3588</Identifier><AffiliationInfo><Affiliation>Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Sofia, Bulgaria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Smith</LastName><ForeName>Sarah E</ForeName><Initials>SE</Initials><AffiliationInfo><Affiliation>MRC Centre for Reproductive Health, The Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Curley</LastName><ForeName>Michael K</ForeName><Initials>MK</Initials><AffiliationInfo><Affiliation>MRC Centre for Reproductive Health, The Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rebourcet</LastName><ForeName>Diane</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>MRC Centre for Reproductive Health, The Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Darbey</LastName><ForeName>Annalucia L</ForeName><Initials>AL</Initials><AffiliationInfo><Affiliation>MRC Centre for Reproductive Health, The Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gannon</LastName><ForeName>Anne-Louise</ForeName><Initials>AL</Initials><AffiliationInfo><Affiliation>MRC Centre for Reproductive Health, The Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sharpe</LastName><ForeName>Richard M</ForeName><Initials>RM</Initials><AffiliationInfo><Affiliation>MRC Centre for Reproductive Health, The Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Smith</LastName><ForeName>Lee B</ForeName><Initials>LB</Initials><AffiliationInfo><Affiliation>MRC Centre for Reproductive Health, The Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK. Lee.Smith@ed.ac.uk.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia. Lee.Smith@ed.ac.uk.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>08</Month><Day>21</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Sci Rep</MedlineTA><NlmUniqueID>101563288</NlmUniqueID><ISSNLinking>2045-2322</ISSNLinking></MedlineJournalInfo><CommentsCorrectionsList><CommentsCorrections RefType="Cites"><RefSource>J Reprod Fertil. 1993 Nov;99(2):395-402</RefSource><PMID Version="1">8107021</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Endocrinology. 1999 Nov;140(11):5364-73</RefSource><PMID Version="1">10537168</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Steroids. 2014 Nov;90:30-8</RefSource><PMID Version="1">25084324</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Reproduction. 2006 Oct;132(4):527-38</RefSource><PMID Version="1">17008464</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Biol Reprod. 2004 Sep;71(3):1016-25</RefSource><PMID Version="1">15163613</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Methods Mol Biol. 2009;530:343-63</RefSource><PMID Version="1">19266339</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Proc Natl Acad Sci U S A. 1996 Oct 1;93(20):10887-90</RefSource><PMID Version="1">8855277</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Reprod Biol Endocrinol. 2012 Jul 21;10:51</RefSource><PMID Version="1">22817830</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Hum Reprod Sci. 2013 Apr;6(2):106-10</RefSource><PMID Version="1">24082651</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nat Genet. 1999 Sep;23(1):99-103</RefSource><PMID Version="1">10471508</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Cell Biol. 2004 Dec 6;167(5):935-44</RefSource><PMID Version="1">15569711</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>PLoS One. 2014 Jan 03;9(1):e84541</RefSource><PMID Version="1">24404170</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nucleic Acids Res. 1999 Nov 15;27(22):4324-7</RefSource><PMID Version="1">10536138</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Methods. 2001 Dec;25(4):402-8</RefSource><PMID Version="1">11846609</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>N Engl J Med. 2003 Feb 13;348(7):618-29</RefSource><PMID Version="1">12584371</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Mol Metab. 2016 May 10;5(7):437-48</RefSource><PMID Version="1">27408770</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nat Rev Drug Discov. 2003 Mar;2(3):205-13</RefSource><PMID Version="1">12612646</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Cell. 2012 Jan 20;148(1-2):33-45</RefSource><PMID Version="1">22265400</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Proc Natl Acad Sci U S A. 2007 Jan 16;104(3):1027-32</RefSource><PMID Version="1">17209010</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Acta Neuropathol Commun. 2015 Nov 26;3:74</RefSource><PMID Version="1">26610346</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Reproduction. 2001 Oct;122(4):553-9</RefSource><PMID Version="1">11570962</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Mol Pharmacol. 1998 Jul;54(1):105-12</RefSource><PMID Version="1">9658195</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Endocrinology. 1999 Dec;140(12):5566-78</RefSource><PMID Version="1">10579320</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Mol Metab. 2015 Aug 29;4(11):771-8</RefSource><PMID Version="1">26629402</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Endocrinology. 2002 Dec;143(12):4922-33</RefSource><PMID Version="1">12446620</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>BMC Dev Biol. 2001;1:4</RefSource><PMID Version="1">11299042</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Reprod Toxicol. 1999 Nov-Dec;13(6):463-72</RefSource><PMID Version="1">10613394</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Genesis. 2002 Nov;34(3):208-14</RefSource><PMID Version="1">12395386</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Int J Androl. 1991 Feb;14(1):66-74</RefSource><PMID Version="1">1672527</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Hum Reprod Update. 2001 May-Jun;7(3):236-47</RefSource><PMID Version="1">11392370</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Natl Cancer Inst. 1998 Jul 1;90(13):967-71</RefSource><PMID Version="1">9665143</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>FASEB J. 2015 Mar;29(3):894-910</RefSource><PMID Version="1">25404712</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Asian J Androl. 2003 Sep;5(3):217-20</RefSource><PMID Version="1">12937805</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Endocrinology. 2002 Dec;143(12):4797-808</RefSource><PMID Version="1">12446607</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Cell. 2010 Oct 1;143(1):134-44</RefSource><PMID Version="1">20887898</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Biochem Biophys Res Commun. 2017 Apr 22;486(1):36-42</RefSource><PMID Version="1">28228354</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Dev Biol. 2002 Apr 15;244(2):305-18</RefSource><PMID Version="1">11944939</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Neuro Endocrinol Lett. 2005 Dec;26(6):729-32</RefSource><PMID Version="1">16380686</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Drug Metab Dispos. 1991 Jan-Feb;19(1):36-43</RefSource><PMID Version="1">1673419</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Exp Toxicol Pathol. 2006 Aug;58(1):1-12</RefSource><PMID Version="1">16709447</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Spermatogenesis. 2011 Oct;1(4):341-353</RefSource><PMID Version="1">22332118</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Mol Endocrinol. 1993 Feb;7(2):232-40</RefSource><PMID Version="1">8469236</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>PLoS One. 2012;7(3):e33529</RefSource><PMID Version="1">22470452</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Neurosci. 2007 Nov 14;27(46):12623-9</RefSource><PMID Version="1">18003841</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Endocrinology. 2011 Feb;152(2):718-29</RefSource><PMID Version="1">21177831</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Androl. 1999 Sep-Oct;20(5):626-34</RefSource><PMID Version="1">10520575</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Dev Dyn. 2006 Dec;235(12):3413-22</RefSource><PMID Version="1">17072878</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Int J Androl. 2003 Feb;26(1):2-15</RefSource><PMID Version="1">12534932</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Gastroenterology. 2012 Jan;142(1):21-24.e7</RefSource><PMID Version="1">22001866</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Endocrinology. 2005 Sep;146(9):4117-26</RefSource><PMID Version="1">15919750</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Mol Cell Endocrinol. 2001 Jun 10;178(1-2):199-206</RefSource><PMID Version="1">11403910</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Biochem Biophys Res Commun. 1997 Aug 28;237(3):752-7</RefSource><PMID Version="1">9299439</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Mol Reprod Dev. 2005 Aug;71(4):516-22</RefSource><PMID Version="1">15892050</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Environ Health Perspect. 1999 May;107(5):397-405</RefSource><PMID Version="1">10210696</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Endocr Rev. 1999 Jun;20(3):253-78</RefSource><PMID Version="1">10368771</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Proc Natl Acad Sci U S A. 2014 May 6;111(18):E1924-32</RefSource><PMID Version="1">24753613</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>FASEB J. 2009 Dec;23(12):4218-30</RefSource><PMID Version="1">19692648</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Reproduction. 2012 Jan 1;143(1):21-33</RefSource><PMID Version="1">22016380</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Steroid Biochem. 1987 Sep;28(3):301-5</RefSource><PMID Version="1">3116344</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Asian J Androl. 2001 Jun;3(2):115-9</RefSource><PMID Version="1">11404795</PMID></CommentsCorrections></CommentsCorrectionsList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>03</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>07</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>8</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>8</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>8</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">28827578</ArticleId><ArticleId IdType="doi">10.1038/s41598-017-09016-4</ArticleId><ArticleId IdType="pii">10.1038/s41598-017-09016-4</ArticleId><ArticleId IdType="pmc">PMC5566418</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/PubMed/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000697 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 000697 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Asie
|area= AustralieFrV1
|flux= PubMed
|étape= Corpus
|type= RBID
|clé= pubmed:28827578
|texte= Low-dose tamoxifen treatment in juvenile males has long-term adverse effects on the reproductive system: implications for inducible transgenics.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:28827578" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a AustralieFrV1
| This area was generated with Dilib version V0.6.33. |  |