Effect of cell cycle arrest on intermediate metabolism in the marine diatom Phaeodactylum tricornutum.
Identifieur interne : 000512 ( PubMed/Corpus ); précédent : 000511; suivant : 000513Effect of cell cycle arrest on intermediate metabolism in the marine diatom Phaeodactylum tricornutum.
Auteurs : Joomi Kim ; Christopher M. Brown ; Min Kyung Kim ; Elizabeth H. Burrows ; Stéphane Bach ; Desmond S. Lun ; Paul G. FalkowskiSource :
- Proceedings of the National Academy of Sciences of the United States of America [ 1091-6490 ] ; 2017.
Abstract
The inhibitor NU 2058 [6-(cyclohexylmethoxy)-9H-purin-2-amine] leads to G1-phase cell cycle arrest in the marine diatom, Phaeodactylum tricornutum, by binding to two cyclin-dependent kinases, CDKA1 and CDKA2. NU 2058 has no effect on photosynthetic attributes, such as Fv/Fm, chlorophyll a/cell, levels of D2 PSII subunits, or RbcL; however, cell cycle arrest leads to unbalanced growth whereby photosynthetic products that can no longer be used for cell division are redirected toward carbohydrates and triacylglycerols (TAGs). Arrested cells up-regulate most genes involved in fatty acid synthesis, including acetyl-CoA carboxylase, and three out of five putative type II diglyceride acyltransferases (DGATs), the enzymes that catalyze TAG production. Correlation of transcriptomes in arrested cells with a flux balance model for P. tricornutum predicts that reactions in the mitochondrion that supply glycerate may support TAG synthesis. Our results reveal that sources of intermediate metabolites and macromolecular sinks are tightly coupled to the cell cycle in a marine diatom, and that arresting cells in the G1 phase leads to remodeling of intermediate metabolism and unbalanced growth.
DOI: 10.1073/pnas.1711642114
PubMed: 28874574
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Brown</name><affiliation><nlm:affiliation>Environmental Biophysics and Molecular Ecology Program, Department of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901.</nlm:affiliation></affiliation></author><author><name sortKey="Kim, Min Kyung" sort="Kim, Min Kyung" uniqKey="Kim M" first="Min Kyung" last="Kim">Min Kyung Kim</name><affiliation><nlm:affiliation>Center for Computational and Integrative Biology, Rutgers, The State University of New Jersey, Camden, NJ 08102.</nlm:affiliation></affiliation></author><author><name sortKey="Burrows, Elizabeth H" sort="Burrows, Elizabeth H" uniqKey="Burrows E" first="Elizabeth H" last="Burrows">Elizabeth H. Burrows</name><affiliation><nlm:affiliation>Department of Chemistry and Chemical Biology, Rutgers, The State University of New Jersey, Piscataway, NJ 08854.</nlm:affiliation></affiliation></author><author><name sortKey="Bach, Stephane" sort="Bach, Stephane" uniqKey="Bach S" first="Stéphane" last="Bach">Stéphane Bach</name><affiliation><nlm:affiliation>Protein Phosphorylation and Human Disease Laboratory, Biological Station, UPMC Univ Paris 06, CNRS USR3151, Sorbonne University, 29688 Roscoff, France.</nlm:affiliation></affiliation></author><author><name sortKey="Lun, Desmond S" sort="Lun, Desmond S" uniqKey="Lun D" first="Desmond S" last="Lun">Desmond S. Lun</name><affiliation><nlm:affiliation>Center for Computational and Integrative Biology, Rutgers, The State University of New Jersey, Camden, NJ 08102.</nlm:affiliation></affiliation></author><author><name sortKey="Falkowski, Paul G" sort="Falkowski, Paul G" uniqKey="Falkowski P" first="Paul G" last="Falkowski">Paul G. 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Brown</name><affiliation><nlm:affiliation>Environmental Biophysics and Molecular Ecology Program, Department of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901.</nlm:affiliation></affiliation></author><author><name sortKey="Kim, Min Kyung" sort="Kim, Min Kyung" uniqKey="Kim M" first="Min Kyung" last="Kim">Min Kyung Kim</name><affiliation><nlm:affiliation>Center for Computational and Integrative Biology, Rutgers, The State University of New Jersey, Camden, NJ 08102.</nlm:affiliation></affiliation></author><author><name sortKey="Burrows, Elizabeth H" sort="Burrows, Elizabeth H" uniqKey="Burrows E" first="Elizabeth H" last="Burrows">Elizabeth H. Burrows</name><affiliation><nlm:affiliation>Department of Chemistry and Chemical Biology, Rutgers, The State University of New Jersey, Piscataway, NJ 08854.</nlm:affiliation></affiliation></author><author><name sortKey="Bach, Stephane" sort="Bach, Stephane" uniqKey="Bach S" first="Stéphane" last="Bach">Stéphane Bach</name><affiliation><nlm:affiliation>Protein Phosphorylation and Human Disease Laboratory, Biological Station, UPMC Univ Paris 06, CNRS USR3151, Sorbonne University, 29688 Roscoff, France.</nlm:affiliation></affiliation></author><author><name sortKey="Lun, Desmond S" sort="Lun, Desmond S" uniqKey="Lun D" first="Desmond S" last="Lun">Desmond S. Lun</name><affiliation><nlm:affiliation>Center for Computational and Integrative Biology, Rutgers, The State University of New Jersey, Camden, NJ 08102.</nlm:affiliation></affiliation></author><author><name sortKey="Falkowski, Paul G" sort="Falkowski, Paul G" uniqKey="Falkowski P" first="Paul G" last="Falkowski">Paul G. Falkowski</name><affiliation><nlm:affiliation>Environmental Biophysics and Molecular Ecology Program, Department of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901; falko@marine.rutgers.edu.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="eISSN">1091-6490</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The inhibitor NU 2058 [6-(cyclohexylmethoxy)-9H-purin-2-amine] leads to G1-phase cell cycle arrest in the marine diatom, Phaeodactylum tricornutum, by binding to two cyclin-dependent kinases, CDKA1 and CDKA2. NU 2058 has no effect on photosynthetic attributes, such as Fv/Fm, chlorophyll a/cell, levels of D2 PSII subunits, or RbcL; however, cell cycle arrest leads to unbalanced growth whereby photosynthetic products that can no longer be used for cell division are redirected toward carbohydrates and triacylglycerols (TAGs). Arrested cells up-regulate most genes involved in fatty acid synthesis, including acetyl-CoA carboxylase, and three out of five putative type II diglyceride acyltransferases (DGATs), the enzymes that catalyze TAG production. Correlation of transcriptomes in arrested cells with a flux balance model for P. tricornutum predicts that reactions in the mitochondrion that supply glycerate may support TAG synthesis. Our results reveal that sources of intermediate metabolites and macromolecular sinks are tightly coupled to the cell cycle in a marine diatom, and that arresting cells in the G1 phase leads to remodeling of intermediate metabolism and unbalanced growth.</div></front></TEI><pubmed><MedlineCitation Status="In-Data-Review" Owner="NLM"><PMID Version="1">28874574</PMID><DateCreated><Year>2017</Year><Month>09</Month><Day>06</Day></DateCreated><DateRevised><Year>2017</Year><Month>10</Month><Day>06</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1091-6490</ISSN><JournalIssue CitedMedium="Internet"><Volume>114</Volume><Issue>38</Issue><PubDate><Year>2017</Year><Month>Sep</Month><Day>19</Day></PubDate></JournalIssue><Title>Proceedings of the National Academy of Sciences of the United States of America</Title><ISOAbbreviation>Proc. Natl. Acad. Sci. U.S.A.</ISOAbbreviation></Journal><ArticleTitle>Effect of cell cycle arrest on intermediate metabolism in the marine diatom Phaeodactylum tricornutum.</ArticleTitle><Pagination><MedlinePgn>E8007-E8016</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1073/pnas.1711642114</ELocationID><Abstract><AbstractText>The inhibitor NU 2058 [6-(cyclohexylmethoxy)-9H-purin-2-amine] leads to G1-phase cell cycle arrest in the marine diatom, Phaeodactylum tricornutum, by binding to two cyclin-dependent kinases, CDKA1 and CDKA2. NU 2058 has no effect on photosynthetic attributes, such as Fv/Fm, chlorophyll a/cell, levels of D2 PSII subunits, or RbcL; however, cell cycle arrest leads to unbalanced growth whereby photosynthetic products that can no longer be used for cell division are redirected toward carbohydrates and triacylglycerols (TAGs). Arrested cells up-regulate most genes involved in fatty acid synthesis, including acetyl-CoA carboxylase, and three out of five putative type II diglyceride acyltransferases (DGATs), the enzymes that catalyze TAG production. Correlation of transcriptomes in arrested cells with a flux balance model for P. tricornutum predicts that reactions in the mitochondrion that supply glycerate may support TAG synthesis. Our results reveal that sources of intermediate metabolites and macromolecular sinks are tightly coupled to the cell cycle in a marine diatom, and that arresting cells in the G1 phase leads to remodeling of intermediate metabolism and unbalanced growth.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Joomi</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Environmental Biophysics and Molecular Ecology Program, Department of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Brown</LastName><ForeName>Christopher M</ForeName><Initials>CM</Initials><AffiliationInfo><Affiliation>Environmental Biophysics and Molecular Ecology Program, Department of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Min Kyung</ForeName><Initials>MK</Initials><AffiliationInfo><Affiliation>Center for Computational and Integrative Biology, Rutgers, The State University of New Jersey, Camden, NJ 08102.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Computer Science, Rutgers, The State University of New Jersey, Camden, NJ 08102.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Burrows</LastName><ForeName>Elizabeth H</ForeName><Initials>EH</Initials><AffiliationInfo><Affiliation>Department of Chemistry and Chemical Biology, Rutgers, The State University of New Jersey, Piscataway, NJ 08854.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bach</LastName><ForeName>Stéphane</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Protein Phosphorylation and Human Disease Laboratory, Biological Station, UPMC Univ Paris 06, CNRS USR3151, Sorbonne University, 29688 Roscoff, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lun</LastName><ForeName>Desmond S</ForeName><Initials>DS</Initials><AffiliationInfo><Affiliation>Center for Computational and Integrative Biology, Rutgers, The State University of New Jersey, Camden, NJ 08102.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Computer Science, Rutgers, The State University of New Jersey, Camden, NJ 08102.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Plant Biology and Pathology, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>School of Information Technology and Mathematical Sciences, University of South Australia, Mawson Lakes, SA 5095, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Falkowski</LastName><ForeName>Paul G</ForeName><Initials>PG</Initials><AffiliationInfo><Affiliation>Environmental Biophysics and Molecular Ecology Program, Department of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901; falko@marine.rutgers.edu.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Protein Phosphorylation and Human Disease Laboratory, Biological Station, UPMC Univ Paris 06, CNRS USR3151, Sorbonne University, 29688 Roscoff, France.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>09</Month><Day>05</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Proc Natl Acad Sci U S A</MedlineTA><NlmUniqueID>7505876</NlmUniqueID><ISSNLinking>0027-8424</ISSNLinking></MedlineJournalInfo><CommentsCorrectionsList><CommentsCorrections RefType="Cites"><RefSource>Annu Rev Cell Dev Biol. 1997;13:261-91</RefSource><PMID Version="1">9442875</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Genome Biol. 2010;11(10):R106</RefSource><PMID Version="1">20979621</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Phycol. 2013 Apr;49(2):381-8</RefSource><PMID Version="1">27008524</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Lipid Res. 2008 May;49(5):1137-46</RefSource><PMID Version="1">18281723</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Biol Chem. 2005 Sep 2;280(35):31208-19</RefSource><PMID Version="1">15975926</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>BMC Plant Biol. 2015 Mar 14;15:86</RefSource><PMID Version="1">25887918</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Anal Chem. 2011 May 15;83(10):3808-16</RefSource><PMID Version="1">21466217</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Plant J. 2015 Dec;84(5):963-73</RefSource><PMID Version="1">26473332</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Med Chem. 2000 Jul 27;43(15):2797-804</RefSource><PMID Version="1">10956187</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Can J Microbiol. 1962 Apr;8:229-39</RefSource><PMID Version="1">13902807</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Science. 1996 Dec 6;274(5293):1652-9</RefSource><PMID Version="1">8939846</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>PLoS One. 2009 Nov 03;4(11):e7743</RefSource><PMID Version="1">19888450</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Proc Natl Acad Sci U S A. 2015 Jan 13;112(2):412-7</RefSource><PMID Version="1">25548193</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Arch Mikrobiol. 1965 May 28;51:94-102</RefSource><PMID Version="1">14347927</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>PLoS One. 2016 Jun 21;11(6):e0157101</RefSource><PMID Version="1">27327084</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Plant J. 2016 Jan;85(1):161-76</RefSource><PMID Version="1">26590126</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Genome Biol. 2010;11(2):R17</RefSource><PMID Version="1">20146805</PMID></CommentsCorrections></CommentsCorrectionsList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">cell cycle</Keyword><Keyword MajorTopicYN="N">diatom</Keyword><Keyword MajorTopicYN="N">flux balance analysis</Keyword><Keyword MajorTopicYN="N">intermediate metabolism</Keyword><Keyword MajorTopicYN="N">unbalanced growth</Keyword></KeywordList><CoiStatement>The authors declare no conflict of interest.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pmc-release"><Year>2018</Year><Month>03</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>9</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>9</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>9</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">28874574</ArticleId><ArticleId IdType="pii">1711642114</ArticleId><ArticleId IdType="doi">10.1073/pnas.1711642114</ArticleId><ArticleId IdType="pmc">PMC5617309</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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