The gut bacterium and pathobiont Bacteroides vulgatus activates NF-κB in a human gut epithelial cell line in a strain and growth phase dependent manner.
Identifieur interne : 000316 ( PubMed/Corpus ); précédent : 000315; suivant : 000317The gut bacterium and pathobiont Bacteroides vulgatus activates NF-κB in a human gut epithelial cell line in a strain and growth phase dependent manner.
Auteurs : Páraic Cuív ; Tomas De Wouters ; Rabina Giri ; Stanislas Mondot ; Wendy J. Smith ; Hervé M. Blottière ; Jakob Begun ; Mark MorrisonSource :
- Anaerobe [ 1095-8274 ] ; 2017.
Abstract
The gut microbiota is increasingly implicated in the pathogenesis of Crohn's disease (CD) and ulcerative colitis (UC) although the identity of the bacteria that underpin these diseases has remained elusive. The pathobiont Bacteroides vulgatus has been associated with both diseases although relatively little is known about how its growth and functional activity might drive the host inflammatory response. We identified an ATP Binding Cassette (ABC) export system and lipoprotein in B. vulgatus ATCC 8482 and B. vulgatus PC510 that displayed significant sequence similarity to an NF-κB immunomodulatory regulon previously identified on a CD-derived metagenomic fosmid clone. Interestingly, the ABC export system was specifically enriched in CD subjects suggesting that it may be important for colonization and persistence in the CD gut environment. Both B. vulgatus ATCC 8482 and PC510 activated NF-κB in a strain and growth phase specific manner in a HT-29/kb-seap-25 enterocyte like cell line. B. vulgatus ATCC 8482 also activated NF-κB in a Caco-2-NF-κBluc enterocyte like and an LS174T-NF-κBluc goblet cell like cell lines, and induced NF-κB-p65 subunit nuclear translocation and IL-6, IL-8, CXCL-10 and MCP-1 gene expression. Despite this, NF-κB activation was not coincident with maximal expression of the ABC exporter or lipoprotein in B. vulgatus PC510 suggesting that the regulon may be necessary but not sufficient for the immunomodulatory effects.
DOI: 10.1016/j.anaerobe.2017.06.002
PubMed: 28583864
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Electronic address: p.ocuiv@uq.edu.au.</nlm:affiliation></affiliation></author><author><name sortKey="De Wouters, Tomas" sort="De Wouters, Tomas" uniqKey="De Wouters T" first="Tomas" last="De Wouters">Tomas De Wouters</name><affiliation><nlm:affiliation>Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France.</nlm:affiliation></affiliation></author><author><name sortKey="Giri, Rabina" sort="Giri, Rabina" uniqKey="Giri R" first="Rabina" last="Giri">Rabina Giri</name><affiliation><nlm:affiliation>Mater Research Institute - The University of Queensland, Translational Research Institute, Brisbane, QLD, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Mondot, Stanislas" sort="Mondot, Stanislas" uniqKey="Mondot S" first="Stanislas" last="Mondot">Stanislas Mondot</name><affiliation><nlm:affiliation>Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France.</nlm:affiliation></affiliation></author><author><name sortKey="Smith, Wendy J" sort="Smith, Wendy J" uniqKey="Smith W" first="Wendy J" last="Smith">Wendy J. Smith</name><affiliation><nlm:affiliation>CSIRO Preventative Health Flagship Research Program, Queensland, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Blottiere, Herve M" sort="Blottiere, Herve M" uniqKey="Blottiere H" first="Hervé M" last="Blottière">Hervé M. Blottière</name><affiliation><nlm:affiliation>Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France; MGP MetaGenoPolis, INRA, Université Paris-Saclay, Jouy-en-Josas, France.</nlm:affiliation></affiliation></author><author><name sortKey="Begun, Jakob" sort="Begun, Jakob" uniqKey="Begun J" first="Jakob" last="Begun">Jakob Begun</name><affiliation><nlm:affiliation>Mater Research Institute - The University of Queensland, Translational Research Institute, Brisbane, QLD, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Morrison, Mark" sort="Morrison, Mark" uniqKey="Morrison M" first="Mark" last="Morrison">Mark Morrison</name><affiliation><nlm:affiliation>The University of Queensland Diamantina Institute, The University of Queensland, Translational Research Institute, Brisbane, QLD, Australia; CSIRO Preventative Health Flagship Research Program, Queensland, Australia.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:28583864</idno><idno type="pmid">28583864</idno><idno type="doi">10.1016/j.anaerobe.2017.06.002</idno><idno type="wicri:Area/PubMed/Corpus">000316</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000316</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The gut bacterium and pathobiont Bacteroides vulgatus activates NF-κB in a human gut epithelial cell line in a strain and growth phase dependent manner.</title><author><name sortKey=" Cuiv, Paraic" sort=" Cuiv, Paraic" uniqKey=" Cuiv P" first="Páraic" last=" Cuív">Páraic Cuív</name><affiliation><nlm:affiliation>The University of Queensland Diamantina Institute, The University of Queensland, Translational Research Institute, Brisbane, QLD, Australia; CSIRO Preventative Health Flagship Research Program, Queensland, Australia. Electronic address: p.ocuiv@uq.edu.au.</nlm:affiliation></affiliation></author><author><name sortKey="De Wouters, Tomas" sort="De Wouters, Tomas" uniqKey="De Wouters T" first="Tomas" last="De Wouters">Tomas De Wouters</name><affiliation><nlm:affiliation>Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France.</nlm:affiliation></affiliation></author><author><name sortKey="Giri, Rabina" sort="Giri, Rabina" uniqKey="Giri R" first="Rabina" last="Giri">Rabina Giri</name><affiliation><nlm:affiliation>Mater Research Institute - The University of Queensland, Translational Research Institute, Brisbane, QLD, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Mondot, Stanislas" sort="Mondot, Stanislas" uniqKey="Mondot S" first="Stanislas" last="Mondot">Stanislas Mondot</name><affiliation><nlm:affiliation>Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France.</nlm:affiliation></affiliation></author><author><name sortKey="Smith, Wendy J" sort="Smith, Wendy J" uniqKey="Smith W" first="Wendy J" last="Smith">Wendy J. Smith</name><affiliation><nlm:affiliation>CSIRO Preventative Health Flagship Research Program, Queensland, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Blottiere, Herve M" sort="Blottiere, Herve M" uniqKey="Blottiere H" first="Hervé M" last="Blottière">Hervé M. Blottière</name><affiliation><nlm:affiliation>Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France; MGP MetaGenoPolis, INRA, Université Paris-Saclay, Jouy-en-Josas, France.</nlm:affiliation></affiliation></author><author><name sortKey="Begun, Jakob" sort="Begun, Jakob" uniqKey="Begun J" first="Jakob" last="Begun">Jakob Begun</name><affiliation><nlm:affiliation>Mater Research Institute - The University of Queensland, Translational Research Institute, Brisbane, QLD, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Morrison, Mark" sort="Morrison, Mark" uniqKey="Morrison M" first="Mark" last="Morrison">Mark Morrison</name><affiliation><nlm:affiliation>The University of Queensland Diamantina Institute, The University of Queensland, Translational Research Institute, Brisbane, QLD, Australia; CSIRO Preventative Health Flagship Research Program, Queensland, Australia.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Anaerobe</title><idno type="eISSN">1095-8274</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The gut microbiota is increasingly implicated in the pathogenesis of Crohn's disease (CD) and ulcerative colitis (UC) although the identity of the bacteria that underpin these diseases has remained elusive. The pathobiont Bacteroides vulgatus has been associated with both diseases although relatively little is known about how its growth and functional activity might drive the host inflammatory response. We identified an ATP Binding Cassette (ABC) export system and lipoprotein in B. vulgatus ATCC 8482 and B. vulgatus PC510 that displayed significant sequence similarity to an NF-κB immunomodulatory regulon previously identified on a CD-derived metagenomic fosmid clone. Interestingly, the ABC export system was specifically enriched in CD subjects suggesting that it may be important for colonization and persistence in the CD gut environment. Both B. vulgatus ATCC 8482 and PC510 activated NF-κB in a strain and growth phase specific manner in a HT-29/kb-seap-25 enterocyte like cell line. B. vulgatus ATCC 8482 also activated NF-κB in a Caco-2-NF-κBluc enterocyte like and an LS174T-NF-κBluc goblet cell like cell lines, and induced NF-κB-p65 subunit nuclear translocation and IL-6, IL-8, CXCL-10 and MCP-1 gene expression. Despite this, NF-κB activation was not coincident with maximal expression of the ABC exporter or lipoprotein in B. vulgatus PC510 suggesting that the regulon may be necessary but not sufficient for the immunomodulatory effects.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">28583864</PMID><DateCreated><Year>2017</Year><Month>06</Month><Day>06</Day></DateCreated><DateRevised><Year>2017</Year><Month>09</Month><Day>29</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1095-8274</ISSN><JournalIssue CitedMedium="Internet"><Volume>47</Volume><PubDate><Year>2017</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Anaerobe</Title><ISOAbbreviation>Anaerobe</ISOAbbreviation></Journal><ArticleTitle>The gut bacterium and pathobiont Bacteroides vulgatus activates NF-κB in a human gut epithelial cell line in a strain and growth phase dependent manner.</ArticleTitle><Pagination><MedlinePgn>209-217</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S1075-9964(17)30120-8</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.anaerobe.2017.06.002</ELocationID><Abstract><AbstractText>The gut microbiota is increasingly implicated in the pathogenesis of Crohn's disease (CD) and ulcerative colitis (UC) although the identity of the bacteria that underpin these diseases has remained elusive. The pathobiont Bacteroides vulgatus has been associated with both diseases although relatively little is known about how its growth and functional activity might drive the host inflammatory response. We identified an ATP Binding Cassette (ABC) export system and lipoprotein in B. vulgatus ATCC 8482 and B. vulgatus PC510 that displayed significant sequence similarity to an NF-κB immunomodulatory regulon previously identified on a CD-derived metagenomic fosmid clone. Interestingly, the ABC export system was specifically enriched in CD subjects suggesting that it may be important for colonization and persistence in the CD gut environment. Both B. vulgatus ATCC 8482 and PC510 activated NF-κB in a strain and growth phase specific manner in a HT-29/kb-seap-25 enterocyte like cell line. B. vulgatus ATCC 8482 also activated NF-κB in a Caco-2-NF-κBluc enterocyte like and an LS174T-NF-κBluc goblet cell like cell lines, and induced NF-κB-p65 subunit nuclear translocation and IL-6, IL-8, CXCL-10 and MCP-1 gene expression. Despite this, NF-κB activation was not coincident with maximal expression of the ABC exporter or lipoprotein in B. vulgatus PC510 suggesting that the regulon may be necessary but not sufficient for the immunomodulatory effects.</AbstractText><CopyrightInformation>Crown Copyright © 2017. Published by Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ó Cuív</LastName><ForeName>Páraic</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>The University of Queensland Diamantina Institute, The University of Queensland, Translational Research Institute, Brisbane, QLD, Australia; CSIRO Preventative Health Flagship Research Program, Queensland, Australia. Electronic address: p.ocuiv@uq.edu.au.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>de Wouters</LastName><ForeName>Tomas</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Giri</LastName><ForeName>Rabina</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Mater Research Institute - The University of Queensland, Translational Research Institute, Brisbane, QLD, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mondot</LastName><ForeName>Stanislas</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Smith</LastName><ForeName>Wendy J</ForeName><Initials>WJ</Initials><AffiliationInfo><Affiliation>CSIRO Preventative Health Flagship Research Program, Queensland, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Blottière</LastName><ForeName>Hervé M</ForeName><Initials>HM</Initials><AffiliationInfo><Affiliation>Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France; MGP MetaGenoPolis, INRA, Université Paris-Saclay, Jouy-en-Josas, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Begun</LastName><ForeName>Jakob</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Mater Research Institute - The University of Queensland, Translational Research Institute, Brisbane, QLD, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Morrison</LastName><ForeName>Mark</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>The University of Queensland Diamantina Institute, The University of Queensland, Translational Research Institute, Brisbane, QLD, Australia; CSIRO Preventative Health Flagship Research Program, Queensland, Australia.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>06</Month><Day>03</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Anaerobe</MedlineTA><NlmUniqueID>9505216</NlmUniqueID><ISSNLinking>1075-9964</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Bacteroides vulgatus</Keyword><Keyword MajorTopicYN="N">Crohn's disease</Keyword><Keyword MajorTopicYN="N">Gut microbiota</Keyword><Keyword MajorTopicYN="N">Intraspecies</Keyword><Keyword MajorTopicYN="N">Metagenomic</Keyword><Keyword MajorTopicYN="N">NF-κB</Keyword><Keyword MajorTopicYN="N">Ulcerative colitis</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>09</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2017</Year><Month>05</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>06</Month><Day>01</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>6</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>6</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>6</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">28583864</ArticleId><ArticleId IdType="pii">S1075-9964(17)30120-8</ArticleId><ArticleId IdType="doi">10.1016/j.anaerobe.2017.06.002</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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