Interaction of CD46 with measles virus: accessory role of CD46 short consensus repeat IV.
Identifieur interne : 003A26 ( PubMed/Checkpoint ); précédent : 003A25; suivant : 003A27Interaction of CD46 with measles virus: accessory role of CD46 short consensus repeat IV.
Auteurs : D. Christiansen [Australie] ; B. Loveland ; P. Kyriakou ; M. Lanteri ; C. Escoffier ; D. GerlierSource :
- The Journal of general virology [ 0022-1317 ] ; 2000.
Descripteurs français
- KwdFr :
- Animaux, Antigènes CD (physiologie), Antigènes CD46, Cellules CHO, Cricetinae, Glycoprotéines membranaires (physiologie), Rougeole (virologie), Récepteurs viraux (physiologie), Régulation négative, Réplication virale, Séquences répétées d'acides nucléiques, Transfection, Virus de la rougeole (physiologie).
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , physiology : Antigens, CD, Membrane Glycoproteins, Receptors, Virus.
- physiology : Measles virus.
- virology : Measles.
- Animals, Antigens, CD46, CHO Cells, Cricetinae, Down-Regulation, Repetitive Sequences, Nucleic Acid, Transfection, Virus Replication.
Abstract
To define further the accessory role(s) of the CD46 (membrane cofactor protein) short consensus repeat (SCR) III and IV domains in the interaction of CD46 with measles virus (MV), chimeric proteins were generated by substituting domains from the structurally related protein decay accelerating factor (DAF, CD55): x3DAF (exchange of CD46 SCR III) and x4DAF (exchange of SCR IV). Transfected CHO cell lines that stably expressed these chimeric proteins were compared for MV binding and infection. Compared with wild-type CD46 (I-II-III-IV), a significant decrease in MV binding was observed with x4DAF. Despite this limited binding, these cells were still capable of supporting virus entry. In a quantitative fusion assay, no significant differences in fusion were observed as a result of the exchange of either CD46 SCR III or IV. However, the down-regulation of cell surface CD46 typically observed following MV infection was abolished with x4DAF, as was the redistribution of CD46 on the cell surface. Thus, CD46 SCR IV appears to be required for optimal virus binding and receptor down-regulation, although importantly, in spite of these functional limitations, x4DAF can still be used for MV entry.
DOI: 10.1099/0022-1317-81-4-911
PubMed: 10725416
Affiliations:
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pubmed:10725416Le document en format XML
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<term>Cricetinae</term>
<term>Down-Regulation</term>
<term>Measles (virology)</term>
<term>Measles virus (physiology)</term>
<term>Membrane Glycoproteins (physiology)</term>
<term>Receptors, Virus (physiology)</term>
<term>Repetitive Sequences, Nucleic Acid</term>
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<term>Cricetinae</term>
<term>Glycoprotéines membranaires (physiologie)</term>
<term>Rougeole (virologie)</term>
<term>Récepteurs viraux (physiologie)</term>
<term>Régulation négative</term>
<term>Réplication virale</term>
<term>Séquences répétées d'acides nucléiques</term>
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<term>Virus de la rougeole</term>
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<term>CHO Cells</term>
<term>Cricetinae</term>
<term>Down-Regulation</term>
<term>Repetitive Sequences, Nucleic Acid</term>
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<term>Cricetinae</term>
<term>Régulation négative</term>
<term>Réplication virale</term>
<term>Séquences répétées d'acides nucléiques</term>
<term>Transfection</term>
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<front><div type="abstract" xml:lang="en">To define further the accessory role(s) of the CD46 (membrane cofactor protein) short consensus repeat (SCR) III and IV domains in the interaction of CD46 with measles virus (MV), chimeric proteins were generated by substituting domains from the structurally related protein decay accelerating factor (DAF, CD55): x3DAF (exchange of CD46 SCR III) and x4DAF (exchange of SCR IV). Transfected CHO cell lines that stably expressed these chimeric proteins were compared for MV binding and infection. Compared with wild-type CD46 (I-II-III-IV), a significant decrease in MV binding was observed with x4DAF. Despite this limited binding, these cells were still capable of supporting virus entry. In a quantitative fusion assay, no significant differences in fusion were observed as a result of the exchange of either CD46 SCR III or IV. However, the down-regulation of cell surface CD46 typically observed following MV infection was abolished with x4DAF, as was the redistribution of CD46 on the cell surface. Thus, CD46 SCR IV appears to be required for optimal virus binding and receptor down-regulation, although importantly, in spite of these functional limitations, x4DAF can still be used for MV entry.</div>
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<Abstract><AbstractText>To define further the accessory role(s) of the CD46 (membrane cofactor protein) short consensus repeat (SCR) III and IV domains in the interaction of CD46 with measles virus (MV), chimeric proteins were generated by substituting domains from the structurally related protein decay accelerating factor (DAF, CD55): x3DAF (exchange of CD46 SCR III) and x4DAF (exchange of SCR IV). Transfected CHO cell lines that stably expressed these chimeric proteins were compared for MV binding and infection. Compared with wild-type CD46 (I-II-III-IV), a significant decrease in MV binding was observed with x4DAF. Despite this limited binding, these cells were still capable of supporting virus entry. In a quantitative fusion assay, no significant differences in fusion were observed as a result of the exchange of either CD46 SCR III or IV. However, the down-regulation of cell surface CD46 typically observed following MV infection was abolished with x4DAF, as was the redistribution of CD46 on the cell surface. Thus, CD46 SCR IV appears to be required for optimal virus binding and receptor down-regulation, although importantly, in spite of these functional limitations, x4DAF can still be used for MV entry.</AbstractText>
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