AustralieFrV1, PubMed, Checkpoint, bibRecord, 003203

SecDF as part of the Sec-translocase facilitates efficient secretion of Bacillus cereus toxins and cell wall-associated proteins.

Identifieur interne : 003203 ( PubMed/Checkpoint ); précédent : 003202; suivant : 003204

SecDF as part of the Sec-translocase facilitates efficient secretion of Bacillus cereus toxins and cell wall-associated proteins.

Auteurs : Aniko Vörös [Norvège] ; Roger Simm [Norvège] ; Leyla Slamti [France] ; Matthew J. Mckay [Australie] ; Ida K. Hegna [Norvège] ; Christina Nielsen-Leroux [France] ; Karl A. Hassan [Australie] ; Ian T. Paulsen [Australie] ; Didier Lereclus [France] ; Ole Andreas Kstad [Norvège] ; Mark P. Molloy [Australie] ; Anne-Brit Kolst [Norvège]

Source :

PloS one [ 1932-6203 ] ; 2014.

RBID : pubmed:25083861

Descripteurs français

KwdFr :
- Analyse de profil d'expression de gènes, Animaux, Bacillus cereus (), Bacillus cereus (physiologie), Bacillus cereus (ultrastructure), Mutation, Papillons de nuit (microbiologie), Paroi cellulaire (métabolisme), Protéines bactériennes (génétique), Protéines bactériennes (métabolisme), Régulation de l'expression des gènes bactériens, Résistance bactérienne aux médicaments (génétique), Stress physiologique, Techniques de knock-out de gènes, Toxines bactériennes (biosynthèse), Transport biologique, Virulence (génétique), Xénobiotique (pharmacologie).
MESH :
- biosynthèse : Toxines bactériennes.
- génétique : Protéines bactériennes, Résistance bactérienne aux médicaments, Virulence.
- microbiologie : Papillons de nuit.
- métabolisme : Paroi cellulaire, Protéines bactériennes.
- pharmacologie : Xénobiotique.
- physiologie : Bacillus cereus.
- Analyse de profil d'expression de gènes, Animaux, Bacillus cereus, Mutation, Régulation de l'expression des gènes bactériens, Stress physiologique, Techniques de knock-out de gènes, Transport biologique.

English descriptors

KwdEn :
- Animals, Bacillus cereus (drug effects), Bacillus cereus (physiology), Bacillus cereus (ultrastructure), Bacterial Proteins (genetics), Bacterial Proteins (metabolism), Bacterial Toxins (biosynthesis), Biological Transport, Cell Wall (metabolism), Drug Resistance, Bacterial (genetics), Gene Expression Profiling, Gene Expression Regulation, Bacterial, Gene Knockout Techniques, Moths (microbiology), Mutation, Stress, Physiological, Virulence (genetics), Xenobiotics (pharmacology).
MESH :
- chemical , biosynthesis : Bacterial Toxins.
- chemical , genetics : Bacterial Proteins.
- drug effects : Bacillus cereus.
- genetics : Drug Resistance, Bacterial, Virulence.
- chemical , metabolism : Bacterial Proteins, Cell Wall.
- microbiology : Moths.
- chemical , pharmacology : Xenobiotics.
- physiology : Bacillus cereus.
- ultrastructure : Bacillus cereus.
- Animals, Biological Transport, Gene Expression Profiling, Gene Expression Regulation, Bacterial, Gene Knockout Techniques, Mutation, Stress, Physiological.

Abstract

The aim of this study was to explore the role of SecDF in protein secretion in Bacillus cereus ATCC 14579 by in-depth characterization of a markerless secDF knock out mutant. Deletion of secDF resulted in pleiotropic effects characterized by a moderately slower growth rate, aberrant cell morphology, enhanced susceptibility to xenobiotics, reduced virulence and motility. Most toxins, including food poisoning-associated enterotoxins Nhe, Hbl, and cytotoxin K, as well as phospholipase C were less abundant in the secretome of the ΔsecDF mutant as determined by label-free mass spectrometry. Global transcriptome studies revealed profound transcriptional changes upon deletion of secDF indicating cell envelope stress. Interestingly, the addition of glucose enhanced the described phenotypes. This study shows that SecDF is an important part of the Sec-translocase mediating efficient secretion of virulence factors in the Gram-positive opportunistic pathogen B. cereus, and further supports the notion that B. cereus enterotoxins are secreted by the Sec-system.

DOI: 10.1371/journal.pone.0103326
PubMed: 25083861

Affiliations:

Links toward previous steps (curation, corpus...)

to stream PubMed, to step Corpus: 003570
to stream PubMed, to step Curation: 003457

Links to Exploration step

pubmed:25083861

Le document en format XML

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<term>Bacillus cereus (drug effects)</term>
<term>Bacillus cereus (physiology)</term>
<term>Bacillus cereus (ultrastructure)</term>
<term>Bacterial Proteins (genetics)</term>
<term>Bacterial Proteins (metabolism)</term>
<term>Bacterial Toxins (biosynthesis)</term>
<term>Biological Transport</term>
<term>Cell Wall (metabolism)</term>
<term>Drug Resistance, Bacterial (genetics)</term>
<term>Gene Expression Profiling</term>
<term>Gene Expression Regulation, Bacterial</term>
<term>Gene Knockout Techniques</term>
<term>Moths (microbiology)</term>
<term>Mutation</term>
<term>Stress, Physiological</term>
<term>Virulence (genetics)</term>
<term>Xenobiotics (pharmacology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term>
<term>Animaux</term>
<term>Bacillus cereus ()</term>
<term>Bacillus cereus (physiologie)</term>
<term>Bacillus cereus (ultrastructure)</term>
<term>Mutation</term>
<term>Papillons de nuit (microbiologie)</term>
<term>Paroi cellulaire (métabolisme)</term>
<term>Protéines bactériennes (génétique)</term>
<term>Protéines bactériennes (métabolisme)</term>
<term>Régulation de l'expression des gènes bactériens</term>
<term>Résistance bactérienne aux médicaments (génétique)</term>
<term>Stress physiologique</term>
<term>Techniques de knock-out de gènes</term>
<term>Toxines bactériennes (biosynthèse)</term>
<term>Transport biologique</term>
<term>Virulence (génétique)</term>
<term>Xénobiotique (pharmacologie)</term>
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</keywords>
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</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Bacillus cereus</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Drug Resistance, Bacterial</term>
<term>Virulence</term>
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<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines bactériennes</term>
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<term>Virulence</term>
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<term>Protéines bactériennes</term>
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<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Bacillus cereus</term>
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<term>Biological Transport</term>
<term>Gene Expression Profiling</term>
<term>Gene Expression Regulation, Bacterial</term>
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<term>Stress, Physiological</term>
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<term>Bacillus cereus</term>
<term>Mutation</term>
<term>Régulation de l'expression des gènes bactériens</term>
<term>Stress physiologique</term>
<term>Techniques de knock-out de gènes</term>
<term>Transport biologique</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The aim of this study was to explore the role of SecDF in protein secretion in Bacillus cereus ATCC 14579 by in-depth characterization of a markerless secDF knock out mutant. Deletion of secDF resulted in pleiotropic effects characterized by a moderately slower growth rate, aberrant cell morphology, enhanced susceptibility to xenobiotics, reduced virulence and motility. Most toxins, including food poisoning-associated enterotoxins Nhe, Hbl, and cytotoxin K, as well as phospholipase C were less abundant in the secretome of the ΔsecDF mutant as determined by label-free mass spectrometry. Global transcriptome studies revealed profound transcriptional changes upon deletion of secDF indicating cell envelope stress. Interestingly, the addition of glucose enhanced the described phenotypes. This study shows that SecDF is an important part of the Sec-translocase mediating efficient secretion of virulence factors in the Gram-positive opportunistic pathogen B. cereus, and further supports the notion that B. cereus enterotoxins are secreted by the Sec-system.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25083861</PMID>
<DateCreated><Year>2014</Year>
<Month>08</Month>
<Day>02</Day>
</DateCreated>
<DateCompleted><Year>2015</Year>
<Month>04</Month>
<Day>23</Day>
</DateCompleted>
<DateRevised><Year>2017</Year>
<Month>02</Month>
<Day>20</Day>
</DateRevised>
<Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Electronic">1932-6203</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>9</Volume>
<Issue>8</Issue>
<PubDate><Year>2014</Year>
</PubDate>
</JournalIssue>
<Title>PloS one</Title>
<ISOAbbreviation>PLoS ONE</ISOAbbreviation>
</Journal>
<ArticleTitle>SecDF as part of the Sec-translocase facilitates efficient secretion of Bacillus cereus toxins and cell wall-associated proteins.</ArticleTitle>
<Pagination><MedlinePgn>e103326</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1371/journal.pone.0103326</ELocationID>
<Abstract><AbstractText>The aim of this study was to explore the role of SecDF in protein secretion in Bacillus cereus ATCC 14579 by in-depth characterization of a markerless secDF knock out mutant. Deletion of secDF resulted in pleiotropic effects characterized by a moderately slower growth rate, aberrant cell morphology, enhanced susceptibility to xenobiotics, reduced virulence and motility. Most toxins, including food poisoning-associated enterotoxins Nhe, Hbl, and cytotoxin K, as well as phospholipase C were less abundant in the secretome of the ΔsecDF mutant as determined by label-free mass spectrometry. Global transcriptome studies revealed profound transcriptional changes upon deletion of secDF indicating cell envelope stress. Interestingly, the addition of glucose enhanced the described phenotypes. This study shows that SecDF is an important part of the Sec-translocase mediating efficient secretion of virulence factors in the Gram-positive opportunistic pathogen B. cereus, and further supports the notion that B. cereus enterotoxins are secreted by the Sec-system.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Vörös</LastName>
<ForeName>Aniko</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Laboratory for Microbial Dynamics (LaMDa), Department of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo, Oslo, Norway.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Simm</LastName>
<ForeName>Roger</ForeName>
<Initials>R</Initials>
<AffiliationInfo><Affiliation>Laboratory for Microbial Dynamics (LaMDa), Department of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo, Oslo, Norway.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Slamti</LastName>
<ForeName>Leyla</ForeName>
<Initials>L</Initials>
<AffiliationInfo><Affiliation>INRA, UMR1319 Micalis, Domaine de La Minière, Guyancourt, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>McKay</LastName>
<ForeName>Matthew J</ForeName>
<Initials>MJ</Initials>
<AffiliationInfo><Affiliation>Australian Proteome Analysis Facility (APAF), Macquarie University, Sydney, Australia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hegna</LastName>
<ForeName>Ida K</ForeName>
<Initials>IK</Initials>
<AffiliationInfo><Affiliation>Laboratory for Microbial Dynamics (LaMDa), Department of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo, Oslo, Norway.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Nielsen-LeRoux</LastName>
<ForeName>Christina</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>INRA, UMR1319 Micalis, Domaine de La Minière, Guyancourt, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hassan</LastName>
<ForeName>Karl A</ForeName>
<Initials>KA</Initials>
<AffiliationInfo><Affiliation>Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, Australia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Paulsen</LastName>
<ForeName>Ian T</ForeName>
<Initials>IT</Initials>
<AffiliationInfo><Affiliation>Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, Australia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Lereclus</LastName>
<ForeName>Didier</ForeName>
<Initials>D</Initials>
<AffiliationInfo><Affiliation>INRA, UMR1319 Micalis, Domaine de La Minière, Guyancourt, France; AgroParistech, UMR Micalis, Jouy-en-Josas, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Økstad</LastName>
<ForeName>Ole Andreas</ForeName>
<Initials>OA</Initials>
<AffiliationInfo><Affiliation>Laboratory for Microbial Dynamics (LaMDa), Department of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo, Oslo, Norway.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Molloy</LastName>
<ForeName>Mark P</ForeName>
<Initials>MP</Initials>
<AffiliationInfo><Affiliation>Australian Proteome Analysis Facility (APAF), Macquarie University, Sydney, Australia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kolstø</LastName>
<ForeName>Anne-Brit</ForeName>
<Initials>AB</Initials>
<AffiliationInfo><Affiliation>Laboratory for Microbial Dynamics (LaMDa), Department of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo, Oslo, Norway.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2014</Year>
<Month>08</Month>
<Day>01</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>PLoS One</MedlineTA>
<NlmUniqueID>101285081</NlmUniqueID>
<ISSNLinking>1932-6203</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D001426">Bacterial Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D001427">Bacterial Toxins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D015262">Xenobiotics</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
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</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>PLoS One. 2011;6(4):e18902</RefSource>
<PMID Version="1">21526150</PMID>
</CommentsCorrections>
</CommentsCorrectionsList>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001409" MajorTopicYN="N">Bacillus cereus</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
<QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName>
<QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001426" MajorTopicYN="N">Bacterial Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001427" MajorTopicYN="N">Bacterial Toxins</DescriptorName>
<QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001692" MajorTopicYN="N">Biological Transport</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D024881" MajorTopicYN="N">Drug Resistance, Bacterial</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020869" MajorTopicYN="N">Gene Expression Profiling</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015964" MajorTopicYN="N">Gene Expression Regulation, Bacterial</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D055786" MajorTopicYN="N">Gene Knockout Techniques</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009036" MajorTopicYN="N">Moths</DescriptorName>
<QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009154" MajorTopicYN="N">Mutation</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013312" MajorTopicYN="N">Stress, Physiological</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014774" MajorTopicYN="N">Virulence</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015262" MajorTopicYN="N">Xenobiotics</DescriptorName>
<QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName>
</MeshHeading>
</MeshHeadingList>
<OtherID Source="NLM">PMC4118872</OtherID>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year>
<Month>03</Month>
<Day>29</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2014</Year>
<Month>06</Month>
<Day>26</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2014</Year>
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<PubMedPubDate PubStatus="pubmed"><Year>2014</Year>
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<PubMedPubDate PubStatus="medline"><Year>2015</Year>
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<ArticleIdList><ArticleId IdType="pubmed">25083861</ArticleId>
<ArticleId IdType="doi">10.1371/journal.pone.0103326</ArticleId>
<ArticleId IdType="pii">PONE-D-14-14114</ArticleId>
<ArticleId IdType="pmc">PMC4118872</ArticleId>
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<affiliations><list><country><li>Australie</li>
<li>France</li>
<li>Norvège</li>
</country>
<region><li>Nouvelle-Galles du Sud</li>
<li>Østlandet</li>
</region>
<settlement><li>Oslo</li>
<li>Sydney</li>
</settlement>
</list>
<tree><country name="Norvège"><region name="Østlandet"><name sortKey="Voros, Aniko" sort="Voros, Aniko" uniqKey="Voros A" first="Aniko" last="Vörös">Aniko Vörös</name>
</region>
<name sortKey=" Kstad, Ole Andreas" sort=" Kstad, Ole Andreas" uniqKey=" Kstad O" first="Ole Andreas" last=" Kstad">Ole Andreas  Kstad</name>
<name sortKey="Hegna, Ida K" sort="Hegna, Ida K" uniqKey="Hegna I" first="Ida K" last="Hegna">Ida K. Hegna</name>
<name sortKey="Kolst, Anne Brit" sort="Kolst, Anne Brit" uniqKey="Kolst A" first="Anne-Brit" last="Kolst">Anne-Brit Kolst</name>
<name sortKey="Simm, Roger" sort="Simm, Roger" uniqKey="Simm R" first="Roger" last="Simm">Roger Simm</name>
</country>
<country name="France"><noRegion><name sortKey="Slamti, Leyla" sort="Slamti, Leyla" uniqKey="Slamti L" first="Leyla" last="Slamti">Leyla Slamti</name>
</noRegion>
<name sortKey="Lereclus, Didier" sort="Lereclus, Didier" uniqKey="Lereclus D" first="Didier" last="Lereclus">Didier Lereclus</name>
<name sortKey="Nielsen Leroux, Christina" sort="Nielsen Leroux, Christina" uniqKey="Nielsen Leroux C" first="Christina" last="Nielsen-Leroux">Christina Nielsen-Leroux</name>
</country>
<country name="Australie"><region name="Nouvelle-Galles du Sud"><name sortKey="Mckay, Matthew J" sort="Mckay, Matthew J" uniqKey="Mckay M" first="Matthew J" last="Mckay">Matthew J. Mckay</name>
</region>
<name sortKey="Hassan, Karl A" sort="Hassan, Karl A" uniqKey="Hassan K" first="Karl A" last="Hassan">Karl A. Hassan</name>
<name sortKey="Molloy, Mark P" sort="Molloy, Mark P" uniqKey="Molloy M" first="Mark P" last="Molloy">Mark P. Molloy</name>
<name sortKey="Paulsen, Ian T" sort="Paulsen, Ian T" uniqKey="Paulsen I" first="Ian T" last="Paulsen">Ian T. Paulsen</name>
</country>
</tree>
</affiliations>
</record>

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	Serveur d'exploration sur les relations entre la France et l'Australie
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Serveur d'exploration sur les relations entre la France et l'Australie

SecDF as part of the Sec-translocase facilitates efficient secretion of Bacillus cereus toxins and cell wall-associated proteins.

SecDF as part of the Sec-translocase facilitates efficient secretion of Bacillus cereus toxins and cell wall-associated proteins.

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