Direct Comparison of Disaccharide Interaction with Lipid Membranes at Reduced Hydrations.
Identifieur interne : 002D10 ( PubMed/Checkpoint ); précédent : 002D09; suivant : 002D11Direct Comparison of Disaccharide Interaction with Lipid Membranes at Reduced Hydrations.
Auteurs : Ben Kent [Allemagne] ; Thomas Hau [Allemagne] ; Bruno Demé [France] ; Viviana Cristiglio [France] ; Tamim Darwish [Australie] ; Taavi Hunt [Australie] ; Gary Bryant [Australie] ; Christopher J. Garvey [Australie]Source :
- Langmuir : the ACS journal of surfaces and colloids [ 1520-5827 ] ; 2015.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Lipid Bilayers, Phosphatidylcholines, Sucrose.
Abstract
Understanding sugar-lipid interactions during desiccation and freezing is an important step in the elucidation of cryo- and anhydro-protection mechanisms. We determine sucrose, trehalose, and water concentration distributions in intra-bilayer volumes between opposing dioleoylphosphatidylcholine bilayers over a range of reduced hydrations and sugar concentrations. Stacked lipid bilayers at reduced hydration provide a suitable system to mimic environmental dehydration effects, as well as a suitable system for direct probing of sugar locations by neutron membrane diffraction. Sugar distributions show that sucrose and trehalose both behave as typical uncharged solutes, largely excluded from the lipid bilayers regardless of sugar identity, and with no correlation between sugar distribution and the lipid headgroup position as the hydration is changed. These results are discussed in terms of current opinions about cryo- and anhydro-protection mechanisms.
DOI: 10.1021/acs.langmuir.5b02127
PubMed: 26225718
Affiliations:
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first="Thomas" last="Hau">Thomas Hau</name><affiliation wicri:level="3"><nlm:affiliation>Institute for Soft Matter and Functional Materials, Helmholtz-Zentrum Berlin , Hahn-Meitner-Platz 1, Berlin D-14109, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute for Soft Matter and Functional Materials, Helmholtz-Zentrum Berlin , Hahn-Meitner-Platz 1, Berlin D-14109</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Deme, Bruno" sort="Deme, Bruno" uniqKey="Deme B" first="Bruno" last="Demé">Bruno Demé</name><affiliation wicri:level="1"><nlm:affiliation>Institute Laue Langevin , Grenoble 38000, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institute Laue Langevin , Grenoble 38000</wicri:regionArea><wicri:noRegion>38000</wicri:noRegion><placeName><settlement 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Institute, Australian Nuclear Science and Technology Organisation , Lucas Heights, NSW 2234, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Bragg Institute, Australian Nuclear Science and Technology Organisation , Lucas Heights, NSW 2234</wicri:regionArea><wicri:noRegion>NSW 2234</wicri:noRegion></affiliation></author><author><name sortKey="Hunt, Taavi" sort="Hunt, Taavi" uniqKey="Hunt T" first="Taavi" last="Hunt">Taavi Hunt</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Molecular and Nanoscale Physics, School of Applied Sciences, RMIT University , Melbourne, VIC 3000, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Centre for Molecular and Nanoscale Physics, School of Applied Sciences, RMIT University , Melbourne, VIC 3000</wicri:regionArea><wicri:noRegion>VIC 3000</wicri:noRegion></affiliation></author><author><name sortKey="Bryant, Gary" sort="Bryant, Gary" uniqKey="Bryant G" first="Gary" last="Bryant">Gary Bryant</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Molecular and Nanoscale Physics, School of Applied Sciences, RMIT University , Melbourne, VIC 3000, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Centre for Molecular and Nanoscale Physics, School of Applied Sciences, RMIT University , Melbourne, VIC 3000</wicri:regionArea><wicri:noRegion>VIC 3000</wicri:noRegion></affiliation></author><author><name sortKey="Garvey, Christopher J" sort="Garvey, Christopher J" uniqKey="Garvey C" first="Christopher J" last="Garvey">Christopher J. Garvey</name><affiliation wicri:level="1"><nlm:affiliation>Bragg Institute, Australian Nuclear Science and Technology Organisation , Lucas Heights, NSW 2234, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Bragg Institute, Australian Nuclear Science and Technology Organisation , Lucas Heights, NSW 2234</wicri:regionArea><wicri:noRegion>NSW 2234</wicri:noRegion></affiliation></author></analytic><series><title level="j">Langmuir : the ACS journal of surfaces and colloids</title><idno type="eISSN">1520-5827</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Lipid Bilayers (chemistry)</term><term>Phosphatidylcholines (chemistry)</term><term>Sucrose (chemistry)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Double couche lipidique ()</term><term>Phosphatidylcholines ()</term><term>Saccharose ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Lipid Bilayers</term><term>Phosphatidylcholines</term><term>Sucrose</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Double couche lipidique</term><term>Phosphatidylcholines</term><term>Saccharose</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Understanding sugar-lipid interactions during desiccation and freezing is an important step in the elucidation of cryo- and anhydro-protection mechanisms. We determine sucrose, trehalose, and water concentration distributions in intra-bilayer volumes between opposing dioleoylphosphatidylcholine bilayers over a range of reduced hydrations and sugar concentrations. Stacked lipid bilayers at reduced hydration provide a suitable system to mimic environmental dehydration effects, as well as a suitable system for direct probing of sugar locations by neutron membrane diffraction. Sugar distributions show that sucrose and trehalose both behave as typical uncharged solutes, largely excluded from the lipid bilayers regardless of sugar identity, and with no correlation between sugar distribution and the lipid headgroup position as the hydration is changed. These results are discussed in terms of current opinions about cryo- and anhydro-protection mechanisms.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26225718</PMID><DateCreated><Year>2015</Year><Month>08</Month><Day>25</Day></DateCreated><DateCompleted><Year>2016</Year><Month>05</Month><Day>23</Day></DateCompleted><DateRevised><Year>2017</Year><Month>11</Month><Day>16</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1520-5827</ISSN><JournalIssue CitedMedium="Internet"><Volume>31</Volume><Issue>33</Issue><PubDate><Year>2015</Year><Month>Aug</Month><Day>25</Day></PubDate></JournalIssue><Title>Langmuir : the ACS journal of surfaces and colloids</Title><ISOAbbreviation>Langmuir</ISOAbbreviation></Journal><ArticleTitle>Direct Comparison of Disaccharide Interaction with Lipid Membranes at Reduced Hydrations.</ArticleTitle><Pagination><MedlinePgn>9134-41</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1021/acs.langmuir.5b02127</ELocationID><Abstract><AbstractText>Understanding sugar-lipid interactions during desiccation and freezing is an important step in the elucidation of cryo- and anhydro-protection mechanisms. We determine sucrose, trehalose, and water concentration distributions in intra-bilayer volumes between opposing dioleoylphosphatidylcholine bilayers over a range of reduced hydrations and sugar concentrations. Stacked lipid bilayers at reduced hydration provide a suitable system to mimic environmental dehydration effects, as well as a suitable system for direct probing of sugar locations by neutron membrane diffraction. Sugar distributions show that sucrose and trehalose both behave as typical uncharged solutes, largely excluded from the lipid bilayers regardless of sugar identity, and with no correlation between sugar distribution and the lipid headgroup position as the hydration is changed. These results are discussed in terms of current opinions about cryo- and anhydro-protection mechanisms.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kent</LastName><ForeName>Ben</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Institute for Soft Matter and Functional Materials, Helmholtz-Zentrum Berlin , Hahn-Meitner-Platz 1, Berlin D-14109, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hauß</LastName><ForeName>Thomas</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Institute for Soft Matter and Functional Materials, Helmholtz-Zentrum Berlin , Hahn-Meitner-Platz 1, Berlin D-14109, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Demé</LastName><ForeName>Bruno</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Institute Laue Langevin , Grenoble 38000, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cristiglio</LastName><ForeName>Viviana</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>Institute Laue Langevin , Grenoble 38000, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Darwish</LastName><ForeName>Tamim</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Bragg Institute, Australian Nuclear Science and Technology Organisation , Lucas Heights, NSW 2234, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hunt</LastName><ForeName>Taavi</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Centre for Molecular and Nanoscale Physics, School of Applied Sciences, RMIT University , Melbourne, VIC 3000, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bryant</LastName><ForeName>Gary</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Centre for Molecular and Nanoscale Physics, School of Applied Sciences, RMIT University , Melbourne, VIC 3000, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Garvey</LastName><ForeName>Christopher J</ForeName><Initials>CJ</Initials><AffiliationInfo><Affiliation>Bragg Institute, Australian Nuclear Science and Technology Organisation , Lucas Heights, NSW 2234, Australia.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>08</Month><Day>11</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Langmuir</MedlineTA><NlmUniqueID>9882736</NlmUniqueID><ISSNLinking>0743-7463</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008051">Lipid Bilayers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010713">Phosphatidylcholines</NameOfSubstance></Chemical><Chemical><RegistryNumber>57-50-1</RegistryNumber><NameOfSubstance UI="D013395">Sucrose</NameOfSubstance></Chemical><Chemical><RegistryNumber>H026DM5V6U</RegistryNumber><NameOfSubstance UI="C017251">1,2-oleoylphosphatidylcholine</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D008051" MajorTopicYN="N">Lipid Bilayers</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010713" MajorTopicYN="N">Phosphatidylcholines</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013395" MajorTopicYN="N">Sucrose</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>7</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>8</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>5</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26225718</ArticleId><ArticleId IdType="doi">10.1021/acs.langmuir.5b02127</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li><li>Australie</li><li>France</li></country><region><li>Auvergne-Rhône-Alpes</li><li>Berlin</li><li>Rhône-Alpes</li></region><settlement><li>Berlin</li><li>Grenoble</li></settlement></list><tree><country name="Allemagne"><region name="Berlin"><name sortKey="Kent, Ben" sort="Kent, Ben" uniqKey="Kent B" first="Ben" last="Kent">Ben Kent</name></region><name sortKey="Hau, Thomas" sort="Hau, Thomas" uniqKey="Hau T" first="Thomas" last="Hau">Thomas Hau</name></country><country name="France"><region name="Auvergne-Rhône-Alpes"><name sortKey="Deme, Bruno" sort="Deme, Bruno" uniqKey="Deme B" first="Bruno" last="Demé">Bruno Demé</name></region><name sortKey="Cristiglio, Viviana" sort="Cristiglio, Viviana" uniqKey="Cristiglio V" first="Viviana" last="Cristiglio">Viviana Cristiglio</name></country><country name="Australie"><noRegion><name sortKey="Darwish, Tamim" sort="Darwish, Tamim" uniqKey="Darwish T" first="Tamim" last="Darwish">Tamim Darwish</name></noRegion><name sortKey="Bryant, Gary" sort="Bryant, Gary" uniqKey="Bryant G" first="Gary" last="Bryant">Gary Bryant</name><name sortKey="Garvey, Christopher J" sort="Garvey, Christopher J" uniqKey="Garvey C" first="Christopher J" last="Garvey">Christopher J. 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