International Laboratory Comparison of Influenza Microneutralization Assays for A(H1N1)pdm09, A(H3N2), and A(H5N1) Influenza Viruses by CONSISE.
Identifieur interne : 002A42 ( PubMed/Checkpoint ); précédent : 002A41; suivant : 002A43International Laboratory Comparison of Influenza Microneutralization Assays for A(H1N1)pdm09, A(H3N2), and A(H5N1) Influenza Viruses by CONSISE.
Auteurs : Karen L. Laurie ; Othmar G. Engelhardt [Royaume-Uni] ; John Wood [Royaume-Uni] ; Alan Heath [Royaume-Uni] ; Jacqueline M. Katz [États-Unis] ; Malik Peiris [Hong Kong] ; Katja Hoschler [Royaume-Uni] ; Olav Hungnes [Norvège] ; Wenqing Zhang [Suisse] ; Maria D. Van Kerkhove [France]Source :
- Clinical and vaccine immunology : CVI [ 1556-679X ] ; 2015.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (sang), Coopération internationale, Furets, Grippe humaine (diagnostic), Grippe humaine (virologie), Humains, Infections à Orthomyxoviridae (diagnostic), Infections à Orthomyxoviridae (médecine vétérinaire), Infections à Orthomyxoviridae (virologie), Reproductibilité des résultats, Sensibilité et spécificité, Sous-type H1N1 du virus de la grippe A (immunologie), Sous-type H5N1 du virus de la grippe A (immunologie), Test ELISA (), Test ELISA (normes), Tests de neutralisation (), Tests de neutralisation (normes).
- MESH :
- diagnostic : Grippe humaine, Infections à Orthomyxoviridae.
- immunologie : Sous-type H1N1 du virus de la grippe A, Sous-type H5N1 du virus de la grippe A.
- médecine vétérinaire : Infections à Orthomyxoviridae.
- normes : Test ELISA, Tests de neutralisation.
- sang : Anticorps antiviraux.
- virologie : Grippe humaine, Infections à Orthomyxoviridae.
- Animaux, Coopération internationale, Furets, Humains, Reproductibilité des résultats, Sensibilité et spécificité, Test ELISA, Tests de neutralisation.
English descriptors
- KwdEn :
- Animals, Antibodies, Viral (blood), Enzyme-Linked Immunosorbent Assay (methods), Enzyme-Linked Immunosorbent Assay (standards), Ferrets, Humans, Influenza A Virus, H1N1 Subtype (immunology), Influenza A Virus, H5N1 Subtype (immunology), Influenza, Human (diagnosis), Influenza, Human (virology), International Cooperation, Neutralization Tests (methods), Neutralization Tests (standards), Orthomyxoviridae Infections (diagnosis), Orthomyxoviridae Infections (veterinary), Orthomyxoviridae Infections (virology), Reproducibility of Results, Sensitivity and Specificity.
- MESH :
- chemical , blood : Antibodies, Viral.
- diagnosis : Influenza, Human, Orthomyxoviridae Infections.
- immunology : Influenza A Virus, H1N1 Subtype, Influenza A Virus, H5N1 Subtype.
- methods : Enzyme-Linked Immunosorbent Assay, Neutralization Tests.
- standards : Enzyme-Linked Immunosorbent Assay, Neutralization Tests.
- veterinary : Orthomyxoviridae Infections.
- virology : Influenza, Human, Orthomyxoviridae Infections.
- Animals, Ferrets, Humans, International Cooperation, Reproducibility of Results, Sensitivity and Specificity.
Abstract
The microneutralization assay is commonly used to detect antibodies to influenza virus, and multiple protocols are used worldwide. These protocols differ in the incubation time of the assay as well as in the order of specific steps, and even within protocols there are often further adjustments in individual laboratories. The impact these protocol variations have on influenza serology data is unclear. Thus, a laboratory comparison of the 2-day enzyme-linked immunosorbent assay (ELISA) and 3-day hemagglutination (HA) microneutralization (MN) protocols, using A(H1N1)pdm09, A(H3N2), and A(H5N1) viruses, was performed by the CONSISE Laboratory Working Group. Individual laboratories performed both assay protocols, on multiple occasions, using different serum panels. Thirteen laboratories from around the world participated. Within each laboratory, serum sample titers for the different assay protocols were compared between assays to determine the sensitivity of each assay and were compared between replicates to assess the reproducibility of each protocol for each laboratory. There was good correlation of the results obtained using the two assay protocols in most laboratories, indicating that these assays may be interchangeable for detecting antibodies to the influenza A viruses included in this study. Importantly, participating laboratories have aligned their methodologies to the CONSISE consensus 2-day ELISA and 3-day HA MN assay protocols to enable better correlation of these assays in the future.
DOI: 10.1128/CVI.00278-15
PubMed: 26108286
Affiliations:
- France, Hong Kong, Norvège, Royaume-Uni, Suisse, États-Unis
- Angleterre, Grand Londres, Géorgie (États-Unis), Île-de-France, Østlandet
- Londres, Oslo, Paris
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pubmed:26108286Le document en format XML
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Laurie</name><affiliation><nlm:affiliation>WHO Collaborating Centre for Reference and Research on Influenza, Victorian Infectious Diseases Reference Laboratory, Peter Doherty Institute for Infection and Immunity, Melbourne, Australia Karen.Laurie@influenzacentre.org.</nlm:affiliation><wicri:noCountry code="subField">Australia Karen.Laurie@influenzacentre.org.</wicri:noCountry></affiliation></author><author><name sortKey="Engelhardt, Othmar G" sort="Engelhardt, Othmar G" uniqKey="Engelhardt O" first="Othmar G" last="Engelhardt">Othmar G. 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Van Kerkhove</name><affiliation wicri:level="3"><nlm:affiliation>Center for Global Health, Institut Pasteur, Paris, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Center for Global Health, Institut Pasteur, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author></analytic><series><title level="j">Clinical and vaccine immunology : CVI</title><idno type="eISSN">1556-679X</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Viral (blood)</term><term>Enzyme-Linked Immunosorbent Assay (methods)</term><term>Enzyme-Linked Immunosorbent Assay (standards)</term><term>Ferrets</term><term>Humans</term><term>Influenza A Virus, H1N1 Subtype (immunology)</term><term>Influenza A Virus, H5N1 Subtype (immunology)</term><term>Influenza, Human (diagnosis)</term><term>Influenza, Human (virology)</term><term>International Cooperation</term><term>Neutralization Tests (methods)</term><term>Neutralization Tests (standards)</term><term>Orthomyxoviridae Infections (diagnosis)</term><term>Orthomyxoviridae Infections (veterinary)</term><term>Orthomyxoviridae Infections (virology)</term><term>Reproducibility of Results</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antiviraux (sang)</term><term>Coopération internationale</term><term>Furets</term><term>Grippe humaine (diagnostic)</term><term>Grippe humaine (virologie)</term><term>Humains</term><term>Infections à Orthomyxoviridae (diagnostic)</term><term>Infections à Orthomyxoviridae (médecine vétérinaire)</term><term>Infections à Orthomyxoviridae (virologie)</term><term>Reproductibilité des résultats</term><term>Sensibilité et spécificité</term><term>Sous-type H1N1 du virus de la grippe A (immunologie)</term><term>Sous-type H5N1 du virus de la grippe A (immunologie)</term><term>Test ELISA ()</term><term>Test ELISA (normes)</term><term>Tests de neutralisation ()</term><term>Tests de neutralisation (normes)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Influenza, Human</term><term>Orthomyxoviridae Infections</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Grippe humaine</term><term>Infections à Orthomyxoviridae</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Sous-type H1N1 du virus de la grippe A</term><term>Sous-type H5N1 du virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term><term>Influenza A Virus, H5N1 Subtype</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term><term>Neutralization Tests</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Infections à Orthomyxoviridae</term></keywords><keywords scheme="MESH" qualifier="normes" xml:lang="fr"><term>Test ELISA</term><term>Tests de neutralisation</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term></keywords><keywords scheme="MESH" qualifier="standards" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term><term>Neutralization Tests</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Orthomyxoviridae Infections</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Grippe humaine</term><term>Infections à Orthomyxoviridae</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Influenza, Human</term><term>Orthomyxoviridae Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Ferrets</term><term>Humans</term><term>International Cooperation</term><term>Reproducibility of Results</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Coopération internationale</term><term>Furets</term><term>Humains</term><term>Reproductibilité des résultats</term><term>Sensibilité et spécificité</term><term>Test ELISA</term><term>Tests de neutralisation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The microneutralization assay is commonly used to detect antibodies to influenza virus, and multiple protocols are used worldwide. These protocols differ in the incubation time of the assay as well as in the order of specific steps, and even within protocols there are often further adjustments in individual laboratories. The impact these protocol variations have on influenza serology data is unclear. Thus, a laboratory comparison of the 2-day enzyme-linked immunosorbent assay (ELISA) and 3-day hemagglutination (HA) microneutralization (MN) protocols, using A(H1N1)pdm09, A(H3N2), and A(H5N1) viruses, was performed by the CONSISE Laboratory Working Group. Individual laboratories performed both assay protocols, on multiple occasions, using different serum panels. Thirteen laboratories from around the world participated. Within each laboratory, serum sample titers for the different assay protocols were compared between assays to determine the sensitivity of each assay and were compared between replicates to assess the reproducibility of each protocol for each laboratory. There was good correlation of the results obtained using the two assay protocols in most laboratories, indicating that these assays may be interchangeable for detecting antibodies to the influenza A viruses included in this study. Importantly, participating laboratories have aligned their methodologies to the CONSISE consensus 2-day ELISA and 3-day HA MN assay protocols to enable better correlation of these assays in the future.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26108286</PMID><DateCreated><Year>2015</Year><Month>07</Month><Day>30</Day></DateCreated><DateCompleted><Year>2016</Year><Month>04</Month><Day>21</Day></DateCompleted><DateRevised><Year>2017</Year><Month>09</Month><Day>22</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1556-679X</ISSN><JournalIssue CitedMedium="Internet"><Volume>22</Volume><Issue>8</Issue><PubDate><Year>2015</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Clinical and vaccine immunology : CVI</Title><ISOAbbreviation>Clin. Vaccine Immunol.</ISOAbbreviation></Journal><ArticleTitle>International Laboratory Comparison of Influenza Microneutralization Assays for A(H1N1)pdm09, A(H3N2), and A(H5N1) Influenza Viruses by CONSISE.</ArticleTitle><Pagination><MedlinePgn>957-64</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1128/CVI.00278-15</ELocationID><Abstract><AbstractText>The microneutralization assay is commonly used to detect antibodies to influenza virus, and multiple protocols are used worldwide. These protocols differ in the incubation time of the assay as well as in the order of specific steps, and even within protocols there are often further adjustments in individual laboratories. The impact these protocol variations have on influenza serology data is unclear. Thus, a laboratory comparison of the 2-day enzyme-linked immunosorbent assay (ELISA) and 3-day hemagglutination (HA) microneutralization (MN) protocols, using A(H1N1)pdm09, A(H3N2), and A(H5N1) viruses, was performed by the CONSISE Laboratory Working Group. Individual laboratories performed both assay protocols, on multiple occasions, using different serum panels. Thirteen laboratories from around the world participated. Within each laboratory, serum sample titers for the different assay protocols were compared between assays to determine the sensitivity of each assay and were compared between replicates to assess the reproducibility of each protocol for each laboratory. There was good correlation of the results obtained using the two assay protocols in most laboratories, indicating that these assays may be interchangeable for detecting antibodies to the influenza A viruses included in this study. Importantly, participating laboratories have aligned their methodologies to the CONSISE consensus 2-day ELISA and 3-day HA MN assay protocols to enable better correlation of these assays in the future.</AbstractText><CopyrightInformation>Copyright © 2015, Laurie et al.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Laurie</LastName><ForeName>Karen L</ForeName><Initials>KL</Initials><AffiliationInfo><Affiliation>WHO Collaborating Centre for Reference and Research on Influenza, Victorian Infectious Diseases Reference Laboratory, Peter Doherty Institute for Infection and Immunity, Melbourne, Australia Karen.Laurie@influenzacentre.org.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Engelhardt</LastName><ForeName>Othmar G</ForeName><Initials>OG</Initials><AffiliationInfo><Affiliation>National Institute for Biological Standards and Control, Medicines and Healthcare Products Regulatory Agency, Potters Bar, Hertfordshire, United Kingdom.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wood</LastName><ForeName>John</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>National Institute for Biological Standards and Control, Hertfordshire, United Kingdom.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Heath</LastName><ForeName>Alan</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>National Institute for Biological Standards and Control, Hertfordshire, United Kingdom.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Katz</LastName><ForeName>Jacqueline M</ForeName><Initials>JM</Initials><AffiliationInfo><Affiliation>Centers for Disease Control and Prevention, Atlanta, Georgia, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Peiris</LastName><ForeName>Malik</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>School of Public Health, The University of Hong Kong, Pok Fu Lam, Hong Kong.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hoschler</LastName><ForeName>Katja</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Public Health England, London, United Kingdom.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hungnes</LastName><ForeName>Olav</ForeName><Initials>O</Initials><AffiliationInfo><Affiliation>Norwegian Institute of Public Health, Oslo, Norway.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Wenqing</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>World Health Organization, Geneva, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Van Kerkhove</LastName><ForeName>Maria D</ForeName><Initials>MD</Initials><AffiliationInfo><Affiliation>Center for Global Health, Institut Pasteur, Paris, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><CollectiveName>CONSISE Laboratory Working Group participants</CollectiveName></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>MR/K010174/1</GrantID><Agency>Medical Research Council</Agency><Country>United Kingdom</Country></Grant><Grant><Agency>Medical Research Council</Agency><Country>United Kingdom</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D016448">Multicenter Study</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>06</Month><Day>24</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Clin Vaccine 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