Visualization of molecular fluorescence point spread functions via remote excitation switching fluorescence microscopy.
Identifieur interne : 002501 ( PubMed/Checkpoint ); précédent : 002500; suivant : 002502Visualization of molecular fluorescence point spread functions via remote excitation switching fluorescence microscopy.
Auteurs : Liang Su [Belgique] ; Gang Lu [Belgique] ; Bart Kenens [Belgique] ; Susana Rocha [Belgique] ; Eduard Fron [Belgique] ; Haifeng Yuan [Belgique] ; Chang Chen [Belgique] ; Pol Van Dorpe [Belgique] ; Maarten B J. Roeffaers [Belgique] ; Hideaki Mizuno [Belgique] ; Johan Hofkens [Danemark] ; James A. Hutchison [Australie] ; Hiroshi Uji-I [Japon]Source :
- Nature communications [ 2041-1723 ] ; 2015.
Abstract
The enhancement of molecular absorption, emission and scattering processes by coupling to surface plasmon polaritons on metallic nanoparticles is a key issue in plasmonics for applications in (bio)chemical sensing, light harvesting and photocatalysis. Nevertheless, the point spread functions for single-molecule emission near metallic nanoparticles remain difficult to characterize due to fluorophore photodegradation, background emission and scattering from the plasmonic structure. Here we overcome this problem by exciting fluorophores remotely using plasmons propagating along metallic nanowires. The experiments reveal a complex array of single-molecule fluorescence point spread functions that depend not only on nanowire dimensions but also on the position and orientation of the molecular transition dipole. This work has consequences for both single-molecule regime-sensing and super-resolution imaging involving metallic nanoparticles and opens the possibilities for fast size sorting of metallic nanoparticles, and for predicting molecular orientation and binding position on metallic nanoparticles via far-field optical imaging.
DOI: 10.1038/ncomms7287
PubMed: 25687887
Affiliations:
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<front><div type="abstract" xml:lang="en">The enhancement of molecular absorption, emission and scattering processes by coupling to surface plasmon polaritons on metallic nanoparticles is a key issue in plasmonics for applications in (bio)chemical sensing, light harvesting and photocatalysis. Nevertheless, the point spread functions for single-molecule emission near metallic nanoparticles remain difficult to characterize due to fluorophore photodegradation, background emission and scattering from the plasmonic structure. Here we overcome this problem by exciting fluorophores remotely using plasmons propagating along metallic nanowires. The experiments reveal a complex array of single-molecule fluorescence point spread functions that depend not only on nanowire dimensions but also on the position and orientation of the molecular transition dipole. This work has consequences for both single-molecule regime-sensing and super-resolution imaging involving metallic nanoparticles and opens the possibilities for fast size sorting of metallic nanoparticles, and for predicting molecular orientation and binding position on metallic nanoparticles via far-field optical imaging.</div>
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<Abstract><AbstractText>The enhancement of molecular absorption, emission and scattering processes by coupling to surface plasmon polaritons on metallic nanoparticles is a key issue in plasmonics for applications in (bio)chemical sensing, light harvesting and photocatalysis. Nevertheless, the point spread functions for single-molecule emission near metallic nanoparticles remain difficult to characterize due to fluorophore photodegradation, background emission and scattering from the plasmonic structure. Here we overcome this problem by exciting fluorophores remotely using plasmons propagating along metallic nanowires. The experiments reveal a complex array of single-molecule fluorescence point spread functions that depend not only on nanowire dimensions but also on the position and orientation of the molecular transition dipole. This work has consequences for both single-molecule regime-sensing and super-resolution imaging involving metallic nanoparticles and opens the possibilities for fast size sorting of metallic nanoparticles, and for predicting molecular orientation and binding position on metallic nanoparticles via far-field optical imaging.</AbstractText>
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<ForeName>Chang</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>1] IMEC, Kapeldreef 75, B-3001 Heverlee, Belgium [2] KU Leuven, Department of Physics and Astronomy, Celestijnenlaan 200D, B-3001 Heverlee, Belgium.</Affiliation>
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<Author ValidYN="Y"><LastName>Van Dorpe</LastName>
<ForeName>Pol</ForeName>
<Initials>P</Initials>
<AffiliationInfo><Affiliation>1] IMEC, Kapeldreef 75, B-3001 Heverlee, Belgium [2] KU Leuven, Department of Physics and Astronomy, Celestijnenlaan 200D, B-3001 Heverlee, Belgium.</Affiliation>
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<ForeName>Maarten B J</ForeName>
<Initials>MB</Initials>
<AffiliationInfo><Affiliation>KU Leuven, Department of Microbial and Molecular Systems, Center for Surface Chemistry and Catalysis, Kasteelpark Arenberg 23, B-3001 Heverlee, Belgium.</Affiliation>
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<Author ValidYN="Y"><LastName>Mizuno</LastName>
<ForeName>Hideaki</ForeName>
<Initials>H</Initials>
<AffiliationInfo><Affiliation>KU Leuven, Department of Chemistry, Celestijnenlaan 200G-F, B-3001 Heverlee, Belgium.</Affiliation>
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<ForeName>Johan</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>1] KU Leuven, Department of Chemistry, Celestijnenlaan 200G-F, B-3001 Heverlee, Belgium [2] University of Copenhagen, Nano-Science Center/Department of Chemistry, Universitetsparken 5, 2100 Copenhagen, Denmark.</Affiliation>
</AffiliationInfo>
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<ForeName>James A</ForeName>
<Initials>JA</Initials>
<AffiliationInfo><Affiliation>1] ISIS &icFRC, Université de Strasbourg &CNRS UMR 7006, Strasbourg 67000, France [2] School of Chemistry and Bio21 Institute, University of Melbourne, Victoria 3010, Australia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Uji-I</LastName>
<ForeName>Hiroshi</ForeName>
<Initials>H</Initials>
<AffiliationInfo><Affiliation>1] KU Leuven, Department of Chemistry, Celestijnenlaan 200G-F, B-3001 Heverlee, Belgium [2] PRESTO, Japan Science and Technology Agency (JST), 4-1-8 Honcho Kawaguchi, Saitama 332-0012, Japan.</Affiliation>
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<Month>02</Month>
<Day>17</Day>
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<CommentsCorrectionsList><CommentsCorrections RefType="Cites"><RefSource>Nano Lett. 2009 Mar;9(3):1189-95</RefSource>
<PMID Version="1">19220005</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Phys Rev Lett. 2005 Dec 16;95(25):257403</RefSource>
<PMID Version="1">16384506</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>J Am Chem Soc. 2008 Dec 24;130(51):17240-1</RefSource>
<PMID Version="1">19049275</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Anal Chem. 2014 Mar 4;86(5):2303-7</RefSource>
<PMID Version="1">24533508</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Biophys J. 2000 Apr;78(4):2151-8</RefSource>
<PMID Version="1">10733992</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Phys Rev Lett. 2005 Jan 21;94(2):023005</RefSource>
<PMID Version="1">15698172</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Nature. 2003 Aug 14;424(6950):824-30</RefSource>
<PMID Version="1">12917696</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Chem Biol. 2006 Oct;13(10):1029-39</RefSource>
<PMID Version="1">17052607</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Nano Lett. 2006 Aug;6(8):1822-6</RefSource>
<PMID Version="1">16895380</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Nat Nanotechnol. 2012 Apr;7(4):237-41</RefSource>
<PMID Version="1">22343380</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Nat Protoc. 2011 Jul;6(7):991-1009</RefSource>
<PMID Version="1">21720313</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Chemphyschem. 2014 Mar 17;15(4):784-93</RefSource>
<PMID Version="1">24254973</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Nat Mater. 2010 Mar;9(3):205-13</RefSource>
<PMID Version="1">20168344</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>PLoS One. 2013;8(2):e55707</RefSource>
<PMID Version="1">23409024</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Adv Protein Chem. 1996;49:57-133</RefSource>
<PMID Version="1">8908297</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Biophys J. 2006 Dec 1;91(11):4258-72</RefSource>
<PMID Version="1">16980368</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Adv Mater. 2014 Aug 13;26(30):5124-8</RefSource>
<PMID Version="1">24866811</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>J Am Chem Soc. 2013 Feb 6;135(5):1845-52</RefSource>
<PMID Version="1">23320465</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Nat Methods. 2006 Oct;3(10):793-5</RefSource>
<PMID Version="1">16896339</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Angew Chem Int Ed Engl. 2008;47(33):6172-6</RefSource>
<PMID Version="1">18646237</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Phys Rev Lett. 2006 Jul 7;97(1):017402</RefSource>
<PMID Version="1">16907406</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Phys Chem Chem Phys. 2013 Mar 28;15(12):4136-45</RefSource>
<PMID Version="1">23138571</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Chemphyschem. 2012 Mar;13(4):973-81</RefSource>
<PMID Version="1">22183928</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>J Biotechnol. 2004 Aug 26;112(1-2):97-107</RefSource>
<PMID Version="1">15288945</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Phys Rev Lett. 2004 Nov 12;93(20):200801</RefSource>
<PMID Version="1">15600907</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Phys Rev Lett. 2006 Mar 24;96(11):113002</RefSource>
<PMID Version="1">16605818</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Nano Lett. 2009 Mar;9(3):995-1001</RefSource>
<PMID Version="1">19199757</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Nat Mater. 2008 Jun;7(6):442-53</RefSource>
<PMID Version="1">18497851</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Adv Mater. 2012 May 2;24(17):2310-4</RefSource>
<PMID Version="1">22467121</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Science. 2006 Sep 15;313(5793):1642-5</RefSource>
<PMID Version="1">16902090</PMID>
</CommentsCorrections>
</CommentsCorrectionsList>
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