Capillary electrophoretic focusing of covalently derivatized protein induced by surfactant.
Identifieur interne : 002228 ( PubMed/Checkpoint ); précédent : 002227; suivant : 002229Capillary electrophoretic focusing of covalently derivatized protein induced by surfactant.
Auteurs : Farid Oukacine [France] ; Joselito P. Quirino [Australie] ; Kiarach Mesbah [France] ; Myriam Taverna [France]Source :
- Electrophoresis [ 1522-2683 ] ; 2016.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Protéines.
- isolement et purification : Protéines.
- Dodécyl-sulfate de sodium, Protéines, Tensioactifs, Électrophorèse capillaire.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Proteins.
- chemical , chemistry : Proteins, Sodium Dodecyl Sulfate, Surface-Active Agents.
- chemical , isolation & purification : Proteins.
- methods : Electrophoresis, Capillary.
Abstract
In this communication, we present a very simple strategy to focus covalently derivatized proteins for high sensitivity CE analysis by LIF detection. We demonstrated that the covalently tagged protein can be focused just by adding SDS at a concentration above the CMC in the derivatized sample. Under specific injection conditions, SDS concentration below the CMC is also sufficient to induce the focusing of the tagged protein. This method allows the quantification and detection of the covalently tagged protein in a narrow zone with an efficiency approaching 220 000 plates/m. Very good linearity was obtained for the ubiquitin in a concentration range of 2-25 μM.
DOI: 10.1002/elps.201600030
PubMed: 26940436
Affiliations:
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Quirino</name><affiliation wicri:level="1"><nlm:affiliation>Australian Centre for Research on Separation Science, School of Physical Sciences-Chemistry, University of Tasmania, Hobart, Tasmania, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Australian Centre for Research on Separation Science, School of Physical Sciences-Chemistry, University of Tasmania, Hobart, Tasmania</wicri:regionArea><wicri:noRegion>Tasmania</wicri:noRegion></affiliation></author><author><name sortKey="Mesbah, Kiarach" sort="Mesbah, Kiarach" uniqKey="Mesbah K" first="Kiarach" last="Mesbah">Kiarach Mesbah</name><affiliation wicri:level="1"><nlm:affiliation>Inst Galien Paris Sud, Fac Pharm, 5 Rue JB Clement, F-92290, Chatenay Malabry, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Inst Galien Paris Sud, Fac Pharm, 5 Rue JB Clement, F-92290, Chatenay Malabry</wicri:regionArea><wicri:noRegion>92290, Chatenay Malabry</wicri:noRegion><wicri:noRegion>92290, Chatenay Malabry</wicri:noRegion></affiliation></author><author><name sortKey="Taverna, Myriam" sort="Taverna, Myriam" uniqKey="Taverna M" first="Myriam" last="Taverna">Myriam Taverna</name><affiliation wicri:level="1"><nlm:affiliation>Inst Galien Paris Sud, Fac Pharm, 5 Rue JB Clement, F-92290, Chatenay Malabry, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Inst Galien Paris Sud, Fac Pharm, 5 Rue JB Clement, F-92290, Chatenay Malabry</wicri:regionArea><wicri:noRegion>92290, Chatenay Malabry</wicri:noRegion><wicri:noRegion>92290, Chatenay Malabry</wicri:noRegion></affiliation></author></analytic><series><title level="j">Electrophoresis</title><idno type="eISSN">1522-2683</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Electrophoresis, Capillary (methods)</term><term>Proteins (analysis)</term><term>Proteins (chemistry)</term><term>Proteins (isolation & purification)</term><term>Sodium Dodecyl Sulfate (chemistry)</term><term>Surface-Active Agents (chemistry)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Dodécyl-sulfate de sodium ()</term><term>Protéines ()</term><term>Protéines (analyse)</term><term>Protéines (isolement et purification)</term><term>Tensioactifs ()</term><term>Électrophorèse capillaire ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Proteins</term><term>Sodium Dodecyl Sulfate</term><term>Surface-Active Agents</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Proteins</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Protéines</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Protéines</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Electrophoresis, Capillary</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Dodécyl-sulfate de sodium</term><term>Protéines</term><term>Tensioactifs</term><term>Électrophorèse capillaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In this communication, we present a very simple strategy to focus covalently derivatized proteins for high sensitivity CE analysis by LIF detection. We demonstrated that the covalently tagged protein can be focused just by adding SDS at a concentration above the CMC in the derivatized sample. Under specific injection conditions, SDS concentration below the CMC is also sufficient to induce the focusing of the tagged protein. This method allows the quantification and detection of the covalently tagged protein in a narrow zone with an efficiency approaching 220 000 plates/m. Very good linearity was obtained for the ubiquitin in a concentration range of 2-25 μM.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26940436</PMID><DateCreated><Year>2016</Year><Month>05</Month><Day>04</Day></DateCreated><DateCompleted><Year>2017</Year><Month>08</Month><Day>24</Day></DateCompleted><DateRevised><Year>2017</Year><Month>11</Month><Day>05</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1522-2683</ISSN><JournalIssue CitedMedium="Internet"><Volume>37</Volume><Issue>9</Issue><PubDate><Year>2016</Year><Month>05</Month></PubDate></JournalIssue><Title>Electrophoresis</Title><ISOAbbreviation>Electrophoresis</ISOAbbreviation></Journal><ArticleTitle>Capillary electrophoretic focusing of covalently derivatized protein induced by surfactant.</ArticleTitle><Pagination><MedlinePgn>1151-4</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/elps.201600030</ELocationID><Abstract><AbstractText>In this communication, we present a very simple strategy to focus covalently derivatized proteins for high sensitivity CE analysis by LIF detection. We demonstrated that the covalently tagged protein can be focused just by adding SDS at a concentration above the CMC in the derivatized sample. Under specific injection conditions, SDS concentration below the CMC is also sufficient to induce the focusing of the tagged protein. This method allows the quantification and detection of the covalently tagged protein in a narrow zone with an efficiency approaching 220 000 plates/m. Very good linearity was obtained for the ubiquitin in a concentration range of 2-25 μM.</AbstractText><CopyrightInformation>© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Oukacine</LastName><ForeName>Farid</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Université Grenoble Alpes, DPM UMR 5063, CNRS, Grenoble, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Quirino</LastName><ForeName>Joselito P</ForeName><Initials>JP</Initials><AffiliationInfo><Affiliation>Australian Centre for Research on Separation Science, School of Physical Sciences-Chemistry, University of Tasmania, Hobart, Tasmania, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mesbah</LastName><ForeName>Kiarach</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Inst Galien Paris Sud, Fac Pharm, 5 Rue JB Clement, F-92290, Chatenay Malabry, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Taverna</LastName><ForeName>Myriam</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Inst Galien Paris Sud, Fac Pharm, 5 Rue JB Clement, F-92290, Chatenay Malabry, France.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>03</Month><Day>30</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Electrophoresis</MedlineTA><NlmUniqueID>8204476</NlmUniqueID><ISSNLinking>0173-0835</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011506">Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D013501">Surface-Active Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>368GB5141J</RegistryNumber><NameOfSubstance 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MajorTopicYN="Y">chemistry</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Capillary electrophoresis</Keyword><Keyword MajorTopicYN="Y">Derivatization</Keyword><Keyword MajorTopicYN="Y">Online sample concentration</Keyword><Keyword MajorTopicYN="Y">Proteins</Keyword><Keyword MajorTopicYN="Y">Sodium dodecyl sulfate</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>01</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2016</Year><Month>02</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>02</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>3</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>3</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>8</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26940436</ArticleId><ArticleId IdType="doi">10.1002/elps.201600030</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Australie</li><li>France</li></country><region><li>Auvergne-Rhône-Alpes</li><li>Rhône-Alpes</li></region><settlement><li>Grenoble</li></settlement><orgName><li>Université Grenoble-Alpes</li></orgName></list><tree><country name="France"><region name="Auvergne-Rhône-Alpes"><name sortKey="Oukacine, Farid" sort="Oukacine, Farid" uniqKey="Oukacine F" first="Farid" last="Oukacine">Farid Oukacine</name></region><name sortKey="Mesbah, Kiarach" sort="Mesbah, Kiarach" uniqKey="Mesbah K" first="Kiarach" last="Mesbah">Kiarach Mesbah</name><name sortKey="Taverna, Myriam" sort="Taverna, Myriam" uniqKey="Taverna M" first="Myriam" last="Taverna">Myriam Taverna</name></country><country name="Australie"><noRegion><name sortKey="Quirino, Joselito P" sort="Quirino, Joselito P" uniqKey="Quirino J" first="Joselito P" last="Quirino">Joselito P. 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