Morphine decreases the pro-angiogenic interaction between breast cancer cells and macrophages in vitro.
Identifieur interne : 001A41 ( PubMed/Checkpoint ); précédent : 001A40; suivant : 001A42Morphine decreases the pro-angiogenic interaction between breast cancer cells and macrophages in vitro.
Auteurs : Samira Khabbazi [Australie] ; Zeyad D. Nassar [Australie] ; Yannick Goumon [France] ; Marie-Odile Parat [Australie]Source :
- Scientific reports [ 2045-2322 ] ; 2016.
Abstract
Interactions between the various cell types that constitute a solid tumour are essential to the biology of the tumour. We evaluated the effect of morphine on the proangiogenic interaction taking place between macrophages and breast cancer cells in vitro. The conditioned medium (CM) from breast cancer cells co-cultured with macrophages elicited endothelial cell proliferation and tube formation. This effect was inhibited if the co-culture occurred in the presence of morphine. The CM from breast cancer cells or macrophages grown individually, whether or not prepared in the presence of morphine, was ineffective in stimulating EC proliferation or tube formation. Using a mouse antibody array, we identified several angiogenesis-regulating factors differentially expressed in the CM of co-cultured cells prepared in the presence or absence of morphine, amongst which interleukin (IL)-6, tumour necrosis factor (TNF)-α and vascular endothelial growth factor (VEGF)-A. VEGF was induced in both cell types by the co-culture and this was prevented by morphine in a non-naloxone reversible fashion. The effect of CM from co-cultured cells on endothelial tube formation, but not proliferation, was prevented by anti-VEGF neutralizing antibody. Our results indicate that morphine prevents, in part via modulating VEGF-A expression, the pro-angiogenic interaction between macrophages and breast cancer cells.
DOI: 10.1038/srep31572
PubMed: 27514308
Affiliations:
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Woollloongabba QLD 4102</wicri:noRegion></affiliation></author><author><name sortKey="Goumon, Yannick" sort="Goumon, Yannick" uniqKey="Goumon Y" first="Yannick" last="Goumon">Yannick Goumon</name><affiliation wicri:level="1"><nlm:affiliation>CNRS UPR3212, Institut des Neurosciences Cellulaires et Intégratives, Centre National de la Recherche Scientifique and University of Strasbourg, 5 rue Blaise Pascal, 67084 Strasbourg, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>CNRS UPR3212, Institut des Neurosciences Cellulaires et Intégratives, Centre National de la Recherche Scientifique and University of Strasbourg, 5 rue Blaise Pascal, 67084 Strasbourg</wicri:regionArea><wicri:noRegion>67084 Strasbourg</wicri:noRegion><placeName><settlement type="city">Strasbourg</settlement><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Alsace (région administrative)</region></placeName></affiliation></author><author><name sortKey="Parat, Marie Odile" sort="Parat, Marie Odile" uniqKey="Parat M" first="Marie-Odile" last="Parat">Marie-Odile Parat</name><affiliation wicri:level="1"><nlm:affiliation>University of Queensland School of Pharmacy, PACE, 20 Cornwall Street. Woollloongabba QLD 4102, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>University of Queensland School of Pharmacy, PACE, 20 Cornwall Street. Woollloongabba QLD 4102</wicri:regionArea><wicri:noRegion>20 Cornwall Street. Woollloongabba QLD 4102</wicri:noRegion></affiliation></author></analytic><series><title level="j">Scientific reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Interactions between the various cell types that constitute a solid tumour are essential to the biology of the tumour. We evaluated the effect of morphine on the proangiogenic interaction taking place between macrophages and breast cancer cells in vitro. The conditioned medium (CM) from breast cancer cells co-cultured with macrophages elicited endothelial cell proliferation and tube formation. This effect was inhibited if the co-culture occurred in the presence of morphine. The CM from breast cancer cells or macrophages grown individually, whether or not prepared in the presence of morphine, was ineffective in stimulating EC proliferation or tube formation. Using a mouse antibody array, we identified several angiogenesis-regulating factors differentially expressed in the CM of co-cultured cells prepared in the presence or absence of morphine, amongst which interleukin (IL)-6, tumour necrosis factor (TNF)-α and vascular endothelial growth factor (VEGF)-A. VEGF was induced in both cell types by the co-culture and this was prevented by morphine in a non-naloxone reversible fashion. The effect of CM from co-cultured cells on endothelial tube formation, but not proliferation, was prevented by anti-VEGF neutralizing antibody. Our results indicate that morphine prevents, in part via modulating VEGF-A expression, the pro-angiogenic interaction between macrophages and breast cancer cells.</div></front></TEI><pubmed><MedlineCitation Status="In-Data-Review" Owner="NLM"><PMID Version="1">27514308</PMID><DateCreated><Year>2016</Year><Month>08</Month><Day>12</Day></DateCreated><DateRevised><Year>2017</Year><Month>02</Month><Day>20</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2045-2322</ISSN><JournalIssue CitedMedium="Internet"><Volume>6</Volume><PubDate><Year>2016</Year><Month>Aug</Month><Day>12</Day></PubDate></JournalIssue><Title>Scientific reports</Title><ISOAbbreviation>Sci Rep</ISOAbbreviation></Journal><ArticleTitle>Morphine decreases the pro-angiogenic interaction between breast cancer cells and macrophages in vitro.</ArticleTitle><Pagination><MedlinePgn>31572</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1038/srep31572</ELocationID><Abstract><AbstractText>Interactions between the various cell types that constitute a solid tumour are essential to the biology of the tumour. We evaluated the effect of morphine on the proangiogenic interaction taking place between macrophages and breast cancer cells in vitro. The conditioned medium (CM) from breast cancer cells co-cultured with macrophages elicited endothelial cell proliferation and tube formation. This effect was inhibited if the co-culture occurred in the presence of morphine. The CM from breast cancer cells or macrophages grown individually, whether or not prepared in the presence of morphine, was ineffective in stimulating EC proliferation or tube formation. Using a mouse antibody array, we identified several angiogenesis-regulating factors differentially expressed in the CM of co-cultured cells prepared in the presence or absence of morphine, amongst which interleukin (IL)-6, tumour necrosis factor (TNF)-α and vascular endothelial growth factor (VEGF)-A. VEGF was induced in both cell types by the co-culture and this was prevented by morphine in a non-naloxone reversible fashion. The effect of CM from co-cultured cells on endothelial tube formation, but not proliferation, was prevented by anti-VEGF neutralizing antibody. Our results indicate that morphine prevents, in part via modulating VEGF-A expression, the pro-angiogenic interaction between macrophages and breast cancer cells.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Khabbazi</LastName><ForeName>Samira</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>University of Queensland School of Pharmacy, PACE, 20 Cornwall Street. Woollloongabba QLD 4102, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nassar</LastName><ForeName>Zeyad D</ForeName><Initials>ZD</Initials><AffiliationInfo><Affiliation>University of Queensland School of Pharmacy, PACE, 20 Cornwall Street. Woollloongabba QLD 4102, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Goumon</LastName><ForeName>Yannick</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>CNRS UPR3212, Institut des Neurosciences Cellulaires et Intégratives, Centre National de la Recherche Scientifique and University of Strasbourg, 5 rue Blaise Pascal, 67084 Strasbourg, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Parat</LastName><ForeName>Marie-Odile</ForeName><Initials>MO</Initials><AffiliationInfo><Affiliation>University of Queensland School of Pharmacy, PACE, 20 Cornwall Street. Woollloongabba QLD 4102, Australia.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>08</Month><Day>12</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Sci Rep</MedlineTA><NlmUniqueID>101563288</NlmUniqueID><ISSNLinking>2045-2322</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="Cites"><RefSource>Pain. 2015 Aug;156(8):1424-32</RefSource><PMID Version="1">25734987</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>BMC Palliat Care. 2015 Oct 27;14 :53</RefSource><PMID Version="1">26507979</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Cancer Res. 2002 Aug 1;62(15):4491-8</RefSource><PMID 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name="Australie"><noRegion><name sortKey="Khabbazi, Samira" sort="Khabbazi, Samira" uniqKey="Khabbazi S" first="Samira" last="Khabbazi">Samira Khabbazi</name></noRegion><name sortKey="Nassar, Zeyad D" sort="Nassar, Zeyad D" uniqKey="Nassar Z" first="Zeyad D" last="Nassar">Zeyad D. Nassar</name><name sortKey="Parat, Marie Odile" sort="Parat, Marie Odile" uniqKey="Parat M" first="Marie-Odile" last="Parat">Marie-Odile Parat</name></country><country name="France"><region name="Grand Est"><name sortKey="Goumon, Yannick" sort="Goumon, Yannick" uniqKey="Goumon Y" first="Yannick" last="Goumon">Yannick Goumon</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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